CPSF6 (NM_007007) Human Untagged Clone

SKU SC115740

CPSF6 (untagged)-Human cleavage and polyadenylation specific factor 6, 68kDa (CPSF6)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CPSF6
• Synonyms: CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115740 sequence for NM_007007 edited (data generated by NextGen Sequencing); ATGGCGGACGGCGTGGACCACATAGACATTTACGCGGATGTCGGCGAAGAGTTCAACCAG; GAAGCTGAATATGGTGGGCATGATCAGATAGATTTGTATGACGATGTCATATCTCCATCT; GCAAATAATGGAGATGCCCCAGAAGACCGAGATTACATGGATACTCTCCCACCAACTGTT; GGTGATGATGTGGGTAAAGGAGCAGCACCAAATGTTGTCTATACATATACTGGAAAGAGA; ATTGCATTATATATTGGAAATCTAACATGGTGGACAACAGATGAAGACTTAACTGAAGCA; GTTCATTCTTTGGGAGTAAATGATATTTTGGAGATAAAATTTTTTGAAAATCGAGCAAAT; GGCCAGTCAAAGGGGTTTGCCCTTGTTGGTGTTGGATCTGAAGCATCTTCAAAAAAGTTA; ATGGATCTGTTACCTAAAAGAGAACTTCATGGTCAGAATCCTGTTGTAACTCCATGCAAT; AAACAGTTCCTGAGTCAATTTGAAATGCAGTCCAGGAAAACTACACAATCAGGACAAATG; TCTGGGGAAGGTAAAGCTGGTCCTCCAGGAGGCAGTTCCCGTGCAGCATTTCCACAAGGT; GGTAGAGGACGGGGCCGTTTTCCAGGGGCTGTTCCTGGTGGGGACAGATTTCCTGGGCCA; GCAGGACCAGGAGGGCCACCCCCACCTTTTCCAGCTGGACAGACTCCACCACGTCCACCC; TTAGGTCCTCCAGGCCCACCTGGTCCACCAGGTCCTCCACCTCCTGGTCAGGTTCTGCCT; CCTCCTCTAGCTGGGCCTCCTAATCGAGGAGATCGCCCTCCACCACCAGTTCTTTTTCCT; GGACAACCTTTTGGGCAGCCTCCATTGGGTCCACTTCCTCCTGGCCCTCCACCTCCAGTT; CCAGGCTACGGCCCCCCTCCTGGCCCACCACCTCCACAACAGGGACCACCTCCACCTCCA; GGCCCCTTTCCACCTCGTCCACCCGGTCCACTTGGGCCACCCCTTACACTAGCTCCTCCT; CCGCATCTTCCTGGACCACCTCCAGGTGCCCCACCGCCAGCTCCGCATGTGAACCCAGCT; TTCTTTCCTCCACCAACTAACAGTGGCATGCCTACATCAGATAGCCGAGGTCCACCACCA; ACAGATCCATATGGGCGACCTCCACCATATGATAGGGGTGACTATGGCCCCCCTGGAAGG; GAAATGGATACTGCAAGAACGCCATTGAGTGAAGCTGAATTTGAAGAAATCATGAATAGA; AATAGGGCAATCTCAAGCAGTGCTATTTCGAGAGCTGTGTCTGATGCCAGTGCTGGTGAT; TATGGGAGTGCTATTGAGACACTGGTAACTGCAATTTCTTTAATTAAACAATCCAAAGTA; TCTGCTGATGATCGTTGCAAAGTTCTTATTAGTTCTTTGCAAGATTGCCTTCATGGAATT; GAGTCCAAGTCTTATGGTTCTGGATCAAGACGTGAACGATCAAGAGAGAGGGACCATAGT; AGATCACGAGAAAAGAGTCGACGTCATAAATCCCGTAGTAGAGACCGTCATGACGATTAT; TACAGAGAGAGAAGCAGAGAACGAGAGAGGCACCGGGATCGTGACCGAGACCGTGACCGA; GAGCGTGACCGAGAGCGCGAATATCGTCATCGTTAG; ; Clone variation with respect to NM_007007.2; 354 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007007 unedited; NGGTCAATTTTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCGGCGGC; CGAGGCTGAAGGAAGATGGCGGACGGCGTGGACCACATAGACATTTACGCGGATGTCGGC; GAAGAGTTCAACCAGGAAGCTGAATATGGTGGGCATGATCAGATAGATTTGTATGACGAT; GTCATATCTCCATCTGCAAATAATGGAGATGCCCCAGAAGACCGAGATTACATGGATACT; CTCCCACCAACTGTTGGTGATGATGTGGGTAAAGGAGCAGCACCAAATGTTGTCTATACA; TATACTGGAAAGAGAATTGCATTATATATTGGAAATCTAACATGGTGGACAACAGATGAA; GACTTAACTGAAGCAGTTCATTCTTTGGGAGTAAATGATATTTTGGAGATAAAATTTTTT; GAAAATCGAGCAAATGGCCAGTCAAAGGGGTTTGCCCTTGTTGGTGTTGGATCTGAAGCA; TCTTCAAAAAAAGTTAATGGATCTGTTACCTAAAAGAGAACTTCATGGTCAGAATCCTGT; TGTAACTCCATGCAATAAACAGTTCCTGAGTCAATTTGAAATGCAGTCCAGGAAAACTAC; ACAATCAGGACAAATGTCTGGGGAAGGTAAAGCTGGTCCTCCAGGAGGCAGTTCCCGTGC; AGCATTTCCACAAGGTGGTAGAGGACGGNNGCCGTTTTCCAGGGGCTGTTCCTGGTGGNG; ACAGATTTCCTGGGCCAGCAGGACCCAGNAGGCCACCCCCACCTTTTCCAGCTGGACAGA; CTCCACCACGTNCACCCTTTAGTCCTNCAGGCCACCTGGNTCCACCAGTCCTNCACTNCT; GGNNCAGGTCTGCCTCCTNCTCTAGCTGGGCCTNCTATCGNAGAGGACGCCCTCCACCAC; AGTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007007 unedited; CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATATGAAA; AGGTAAATAAAGGATTTAATTAGTATAAAATTTCCAGTTTCATTTCAAGTGCAGTTAGAT; TTTTGTGATTCCATTTTTAAACTGTTCAAAGAAAATTTTATTTTCATGGACATTTTACTT; ACCTTTTAACCCTTTACTATTAACTAATTTCTCTTGTAATATTTATATGTCCATACTTCT; TCGCATTAGGGCATTGTAGTCAAAATATTAAAAAGCCAAGAAATGACACAACAATATACA; GAAAACAGGAAAATTTTGATACAACTCTAAAATAATGTTAAAAAAGCAACCGCATTTTCA; ACAAAATGCTTTAAAAAATCATTTCTGAAAGTGAACAGTTCTGATAAACATCAACGATCC; CATGCACAGATACTTCAAACACAAAATATTTTAAAATAAAACTGGCAGTGATATGGGTAA; TTCAAAACAGCTAATTTTGTCAGAAGTGGTTTGATAGCAGACAAGATGAAAGTGAAGAAC; AGCAGTGGAAATTTCTGGAGGGTTTCTACAGTTGCACATAACACAGTATCTAGGGTATTT; TAACACTTTAAGCATTGTCCTAGAATTTGGGTACTCTTTAACAACCAATGTGTATTAACA; GAAGATCTCTCANAGATAATCAGATCTTAAAAACATATTAACGTGCTCAAGAATTTTAAG; TCAAATTTCTGAAGTAGTGATTTCAAATTACATTCCCATAAAAACACCTAATCAAGTTGA; CACCCTGCCTCTGCNCATNNTTGCATCAAGACACATGCTAGTCANGTGCTTTTAAAGTGG; GCCTATATGTAAGGCAGGGTTGAAAAAATCTTTNGATTAGTACTATGCATGATCAATCCG; GGCATGAATATCATANATCCTTAACTGNGCGACCCGTTTATCTGTATTNCCATACAAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_007007
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007007.1, NP_008938.1
• RefSeq Size: 3426 bp
• RefSeq ORF: 1656 bp
• Locus ID: 11052
• UniProt ID: Q16630
• Cytogenetics: 12q15
• Domains: RRM
• Summary: The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) lacks an alternate in-frame exon in the central coding region, compared to variant 2. The encoded isoform (1) is shorter than isoform 2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.