NONO (NM_007363) Human Untagged Clone

CAT#: SC115563

NONO (untagged)-Human non-POU domain containing, octamer-binding (NONO), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NONO
• Synonyms: MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115563 sequence for NM_007363 edited (data generated by NextGen Sequencing)
  ATGCAGAGTAATAAAACTTTTAACTTGGAGAAGCAAAACCATACTCCAAGAAAGCATCAT
  CAACATCACCACCAGCAGCAGCACCACCAGCAGCAACAGCAGCAGCCGCCACCACCGCCA
  ATACCTGCAAATGGGCAACAGGCCAGCAGCCAAAATGAAGGCTTGACTATTGACCTGAAG
  AATTTTAGAAAACCAGGAGAGAAGACCTTCACCCAACGAAGCCGTCTTTTTGTGGGAAAT
  CTTCCTCCCGACATCACTGAGGAAGAAATGAGGAAACTATTTGAGAAATATGGAAAGGCA
  GGCGAAGTCTTCATTCATAAGGATAAAGGATTTGGCTTTATCCGCTTGGAAACCCGAACC
  CTAGCGGAGATTGCCAAAGTGGAGCTGGACAATATGCCACTCCGTGGAAAGCAGCTGCGT
  GTGCGCTTTGCCTGCCATAGTGCATCCCTTACAGTTCGAAACCTTCCTCAGTATGTGTCC
  AACGAACTGCTGGAAGAAGCCTTTTCTGTGTTTGGCCAGGTAGAGAGGGCTGTAGTCATT
  GTGGATGATCGAGGAAGGCCCTCAGGAAAAGGCATTGTTGAGTTCTCAGGGAAGCCAGCT
  GCTCGGAAAGCTCTGGACAGATGCAGTGAAGGCTCCTTCCTGCTAACCACATTTCCTCGT
  CCTGTGACTGTGGAGCCCATGGACCAGTTAGATGATGAAGAGGGACTTCCAGAGAAGCTG
  GTTATAAAAAACCAGCAATTTCACAAGGAACGAGAGCAGCCACCCAGATTTGCACAGCCT
  GGCTCCTTTGAGTATGAATATGCCATGCGCTGGAAGGCACTCATTGAGATGGAGAAGCAG
  CAGCAGGACCAAGTGGACCGCAACATCAAGGAGGCTCGTGAGAAGCTGGAGATGGAGATG
  GAAGCTGCACGCCATGAGCACCAGGTCATGCTAATGAGACAGGATTTGATGAGGCGCCAA
  GAAGAACTTCGGAGGATGGAAGAGCTGCACAACCAAGAGGTGCAAAAACGAAAGCAACTG
  GAGCTCAGGCAGGAGGAAGAGCGCAGGCGCCGTGAAGAAGAGATGCGGCGGCAGCAAGAA
  GAAATGATGCGGCGACAGCAGGAAGGATTCAAGGGAACCTTCCCTGATGCGAGAGAGCAG
  GAGATTCGGATGGGTCAGATGGCTATGGGAGGTGCTATGGGCATAAACAACAGAGGTGCC
  ATGCCCCCTGCTCCTGTGCCAGCTGGTACCCCAGCTCCTCCAGGACCTGCCACTATGATG
  CCGGATGGAACTTTGGGATTGACCCCACCAACAACTGAACGCTTTGGTCAGGCTGCTACA
  ATGGAAGGAATTGGGGCAATTGGTGGAACTCCTCCTGCATTCAACCGTGCAGCTCCTGGA
  GCTGAATTTGCCCCAAACAAACGTCGCCGATACTAA
  
  Clone variation with respect to NM_007363.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007363 unedited
  CCACACCCTTCGCACGAGGGGCCGTGTAGTTTCGCCGTTACTCCGAGGAGAAACCAGTCG
  GTAGAGGAGAAGTCGAGGTTAGAGGGAACTGGGAGGCACTTTGCTGTCTGCAATCGAAGT
  TGAGGGTGCAAAAATGCAGAGTAATAAAACTTTTAACTTGGAGAAGCAAAACCATACTCC
  AAGTAAGCATCATCAACATCACCACCTGCAGCAGCACCACCAGCAGCAACAGCAGCAGCC
  GCCACCACCGCCAATACCTGCAAATGGGCAACAGGCCAGCAGCCAAAATGAAGGCTTGAC
  TATTGACCTGAAGAATTTTAGAAAACCAGGAGAGAAGACCTTCACCCAACGAAGCCGTCT
  TTTTGTGGGAAATCTTCCTCCCGACATCACTGAGGAAGAAATGAGGAAACTATTTGAGAA
  ATATGGAAAGGCAGGCGAAGTCTTCATTCATAAGGATAAAGGATTTGGCTTTATCCGCTT
  GGAAACCCGAACCCTAGCGGAGATTGCCAAAGTGGAGCTGGACAATATGCCACTCCGTGG
  AAAGCAGCTGCGTGTGCGCTTTGCCTGCCATAGTGCATCCCTTACAGTTCGAAACCTTCC
  TCAGTATGTGTCCAACGAACTGCTGGAAGAAGCCTTTTCTGTGTTTGGCCAGGTAGAGAG
  GGCTGTAGTCATTGTGGATGATCGAGGAAGGCCCCTCAGGAAAGGCATTGTTGAGTTCTC
  AGGGAAGCCAGCTGCTCGGAAAGCTCTGGACAGATGCAGTGAAGGGCTCTTNCTGCTAAC
  CACATTTCCTCGTCCTGTGACTGTGGAGCCCATGGCCCATTAGATGATGAAGAGGNACTT
  NCAGAGAAGCTGGTTTATAAAAAACAGCAATTTCACAAGGAACGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007363 unedited
  TATGGACCGCGGGCCGCAATCTANAATCGAGTTTTTTTTTTTTTTTTTTAAAAGGCTTAA
  AATGTATTTTAATAGGTTGATGCTGCATTACTGCTCCATTTTTCAGAAAAACTCCAAATA
  TATGAGACAAATCAAACACAGTACACCAATGAAAAAAAAAATGCACAGCATGACTACCTA
  AGATAGGCGAGTCTAAGAGCTACTGTCAGACCTTCAGTATACAGTAACCCTGTTCCCAAG
  ATTGTACTAGGCCTATCAAGAAAAGCTGTGAACAGCAACATCATAGACACAAAAGGGTCA
  TTCCTGGGTGTCCTCACCCAAGAAAAAGATGGAGGCAAGTTAACACAAGATTTTTTTTTA
  AAGATACACTAAAATGAAAATCTCTAAGAGAAAATGTCTTCTTTAGGACATGAAGGGGTA
  ATATATGGGGTATAACAGAACCGTATGTACGCTGCCTGTGACCTTTGTGGACGTTTGCCT
  GAATTCTCACCTGGGAGTGAATGGAGCAATGGGGCTAAGGTCATTGGGGGATGTTTGGTT
  TTTGTGGTGATGTNGGCAGTTTCTCTGGGGCTGGGGGAGTGTAAGGATAAGAAGCAAGTG
  AGGTTGGGCACGGTGGCTCACCCCTGTAATCCCAGCATTTTGGGAGGCCGAGGTGGGTAG
  ATCACGAGGTCAGGAGTTCAAGATCAACCTGGACAAGCTTGAACCCGGGAGGCAGAGATT
  GCAGTGAGCCGAGATCATGCCACTACACTCCAGCCTGGGCGACAGAGTAAGACTCCGTCT
  CGGGGGGGGCGGGGCANGCAAATGAAACAAGATGGACANAAAAAAAAAACCGGNATGATN
  CAGGAATAATGGCTCTAANCTGGNANCACTTNGGCCATGGGTTCCCCCAGCCCTCACAGT
  CCATTTAGAACTTTNAGGATGCACANACAGAACTGATAANNCATTACACACGNATTGGGC
  TTGTTAATACACCNTNCTTGNCTCTGCTCACATCTCCTTAAGTTCAGCCGGGGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007363
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007363.3, NP_031389.3
• RefSeq Size: 2705 bp
• RefSeq ORF: 1416 bp
• Locus ID: 4841
• UniProt ID: Q15233
• Cytogenetics: Xq13.1
• Domains: RRM
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).