BACE1 (NM_012104) Human Untagged Clone

CAT#: SC115547

BACE1 (untagged)-Human beta-site APP-cleaving enzyme 1 (BACE1), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BACE1
• Synonyms: ASP2; BACE; HSPC104
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115547 sequence for NM_012104 edited (data generated by NextGen Sequencing)
  ATGGCCCAAGCCCTGCCCTGGCTCCTGCTGTGGATGGGCGCGGGAGTGCTGCCTGCCCAC
  GGCACCCAGCACGGCATCCGGCTGCCCCTGCGCAGCGGCCTGGGGGGCGCCCCCCTGGGG
  CTGCGGCTGCCCCGGGAGACCGACGAAGAGCCCGAGGAGCCCGGCCGGAGGGGCAGCTTT
  GTGGAGATGGTGGACAACCTGAGGGGCAAGTCGGGGCAGGGCTACTACGTGGAGATGACC
  GTGGGCAGCCCCCCGCAGACGCTCAACATCCTGGTGGATACAGGCAGCAGTAACTTTGCA
  GTGGGTGCTGCCCCCCACCCCTTCCTGCATCGCTACTACCAGAGGCAGCTGTCCAGCACA
  TACCGGGACCTCCGGAAGGGTGTGTATGTGCCCTACACCCAGGGCAAGTGGGAAGGGGAG
  CTGGGCACCGACCTGGTAAGCATCCCCCATGGCCCCAACGTCACTGTGCGTGCCAACATT
  GCTGCCATCACTGAATCAGACARGTTCTTCATCAACGGCTCCAACTGGGAAGGCATCCTG
  GGGCTGGCCTATGCTGAGATTGCCAGGCCTGACGACTCCCTGGAGCCTTTCTTTGACTCT
  CTGGTAAAGCAGACCCACGTTCCCAACCTCTTCTCCCTGCAGCTTTGTGGTGCTGGCTTC
  CCCCTCAACCAGTCTGAAGTGCTGGCCTCTGTCGGAGGGAGCATGATCATTGGAGGTATC
  GACCACTCGCTGTACACAGGCAGTCTCTGGTATACACCCATCCGGCGGGAGTGGTATTAT
  GAGGTGATCATTGTGCGGGTGGAGATCAATGGACAGGATCTGAAAATGGACTGCAAGGAG
  TACAACTATGACAAGAGCATTGTGGACAGTGGCACCACCAACCTTCGTTTGCCCAAGAAA
  GTGTTTGAAGCTGCAGTCAAATCCATCAAGGCAGCCTCCTCCACGGAGAAGTTCCCTGAT
  GGTTTCTGGCTAGGAGAGCAGCTGGTGTGCTGGCAAGCAGGCACCACCCCTTGGAACATT
  TTCCCAGTCATCTCACTCTACCTAATGGGTGAGGTTACCAACCAGTCCTTCCGCATCACC
  ATCCTTCCGCAGCAATACCTGCGGCCAGTGGAAGATGTGGCCACGTCCCAAGACGACTGT
  TACAAGTTTGCCATCTCACAGTCATCCACGGGCACTGTTATGGGAGCTGTTATCATGGAG
  GGCTTCTACGTTGTCTTTGATCGGGCCCGAAAACGAATTGGCTTTGCTGTCAGCGCTTGC
  CATGTGCACGATGAGTTCAGGACGGCAGCGGTGGAAGGCCCTTTTGTCACCTTGGACATG
  GAAGACTGTGGCTACAACATTCCACAGACAGATGAGTCAACCCTCATGACCATAGCCTAT
  GTCATGGCTGCCATCTGCGCCCTCTTCATGCTGCCACTCTGCCTCATGGTGTGTCAGTGG
  CGCTGCCTCCGCTGCCTGCGCCAGCAGCATGATGACTTTGCTGATGACATCTCCCTGCTG
  AAGTGA
  
  Clone variation with respect to NM_012104.4
  503 a=>r
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012104 unedited
  GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGACCACCCAGACTTGGGGG
  CAGGCGCCAGGGACGGACGTGGGCCAGTGCGAGCCCAGAGGGCCCGAAGGCCGGGGCCCA
  CCATGGCCCAAGCCCTGCCCTGGCTCCTGCTGTGGATGGGCGCGGGAGTGCTGCCTGCCC
  ACGGCACCCAGCACGGCATCCGGCTGCCCCTGCGCAGCGGCCTGGGGGGCGCCCCCCTGG
  GGCTGCGGCTGCCCCGGGAGACCGACGAAGAGCCCGAGGAGCCCGGCCGGAGGGGCAGCT
  TTGTGGAGATGGTGGACAACCTGAGGGGCAAGTCGGGGCAGGGCTACTACGTGGAGATGA
  CCGTGGGCAGCCCCCCGCAGACGCTCAACATCCTGGTGGATACAGGCAGCAGTAACTTTG
  CAGTGGGTGCTGCCCCCCACCCCTTCCTGCATCGCTACTACCAGAGGCAGCTGTCCAGCA
  CATACCGGGACCTCCGGAAGGGTGTGTATGTGCCCTACACCCAGGGCAAGTGGGAAGGGG
  AGCTGGGCACCGACCTGGTAAGCATCCCCCATGGCCCCAACGTCACTGTGCGTGCCAACA
  TTGCTGCCATCACTGAATCAGACAAGTTCTTCATCAACGGCTCCAACTGGGAAGGCATCC
  TGGGGCTGGCCTATGCTGAGATTGCCAGGCCTGACGACTCCCTGGAGCCTTTCTTTGACT
  CTCTGGTAAAGCAGACCCACGTTCCCAACCTCTTCTCCCTGCAGCTTTGTGGGTGCCTGG
  CCTTCCCCTCCACCAGTCTGAAGTGCTGTCCTCTGTCGGAGGGAGCATGATCATTGGAGG
  TATCGACCACTCCCTGTACACAAGCAGTCTCTGGTATACACCCATCCGCGGGAGTGTATT
  ATGAAGGGATCATTTGGCGGTGGAACATCATGGACACGACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012104 unedited
  CTTACTTGNNACCGCGGCCGCTATCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAAACT
  GACTCAGTATTCTTGTTTTATTGGTTTATGGCTTGTGGGCAGCTGGCTGAGGCCAGGAAC
  ACCAGAGGCCCTCCTTGTATTTCCCTCTCCCTTATCAGGTAATTCCACACTTAGCAGGTC
  CCATACCTGGAAGCAGCGGGTTGACCAGGTAGGAGTAGGATGCAGTGGGTATACTAGGAA
  AACCCAGCTCTTCCCACTAACACTGGTACCGTAGTTTACTATGCTAGAACTGATAGGGAG
  GTAATGAACTTGGTCCCCACTACCTAGCCCAAGAACCAGCCTGGGTACCCACGTACCATT
  AGGAGCGGGGAGGGGAAGAACAGGATAGGGGAAAAAGATGACCTTCATCAAGGAGCTCTT
  GTGGTGGAGGACATAAGGAAGCCCTGAGGCTGCCATCCTTTCTCCAGGCAGCCTTGATAC
  TGAAGGCAGTAATGTTAGAGCCATAAAGCACTGTATTTCTCATACTCTTGGTATAATGCT
  AGTGCCACTGTGTGATGCTTTAGTCAAATAAATGGAGGGGCCATTANAGAGGGTAGGAAG
  GCAATGAAACCACTCTGAATTATGTAAAGATCTTGCTACTTCTTATAGCCTAATAGGAAG
  AAATCCAGGCAGGGGTTATAGGGCATCAGCTGTTGAATGAATGTTCAACATTATGCTNTT
  AAGGATTAGATAGAAGAAGTCTNCTACCTATTCCTTTTAGCACCTGGNCTGCAAAGCTCC
  AGAGGAAGAAATTGGGGAAAGAGTGAGCCTATGACCCAGAAGACAGAGCCCAGCCCTGCT
  ATAGTCCAGTTGCTCTCCCACAGGCACCTGGAGCTTAGGGGGNNTAGAATTTCAGTGCTG
  CCTTCATATGTAGATAAAGGGTGGTCAGAAGAGACATGAAATTATATGCNGAAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012104
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012104.3, NP_036236.1
• RefSeq Size: 5850 bp
• RefSeq ORF: 1506 bp
• Locus ID: 23621
• UniProt ID: P56817
• Cytogenetics: 11q23.3
• Domains: asp
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease
• Gene Summary: This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (a) is the longest transcript and it encodes the longest isoform (A).