APPL (APPL1) (NM_012096) Human Untagged Clone

CAT#: SC115543

APPL1 (untagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APPL
• Synonyms: APPL; DIP13alpha; MODY14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115543 sequence for NM_012096 edited (data generated by NextGen Sequencing)
  ATGCCGGGGATCGACAAGCTGCCCATCGAGGAGACGCTGGAGGACAGCCCGCAGACAAGG
  TCTTTACTAGGTGTATTTGAAGAAGATGCCACAGCTATTTCCAACTATATGAACCAGTTG
  TATCAAGCTATGCATCGGATTTATGATGCACAGAATGAATTAAGTGCAGCAACACACCTG
  ACCTCAAAACTTTTAAAAGAATATGAAAAACAGCGTTTTCCATTGGGAGGTGATGATGAA
  GTTATGAGCTCTACATTGCAACAGTTTTCAAAAGTTATAGATGAGCTTAGCTCTTGTCAT
  GCAGTGCTTTCAACTCAACTTGCTGATGCCATGATGTTCCCCATTACCCAGTTTAAAGAA
  AGAGATCTGAAAGAAATACTAACATTAAAGGAAGTATTTCAGATTGCAAGTAATGATCAT
  GATGCTGCGATTAATAGATATAGCCGTTTATCAAAAAAAAGAGAAAATGACAAGGTGAAG
  TATGAAGTAACAGAAGATGTGTACACATCCAGAAAGAAACAACACCAGACCATGATGCAT
  TATTTTTGTGCATTAAATACTCTTCAGTACAAGAAGAAAATAGCATTGTTAGAACCTCTA
  CTTGGGTACATGCAAGCTCAGATAAGTTTCTTTAAGATGGGTTCTGAAAATCTTAATGAA
  CAACTGGAAGAATTTTTAGCTAATATTGGAACAAGCGTTCAGAATGTTCGCAGGGAAATG
  GACAGTGATATAGAGACCATGCAACAGACAATAGAGGATTTGGAAGTAGCCAGTGATCCC
  TTATATGTGCCTGACCCAGACCCCACCAAATTTCCTGTTAATCGAAATTTAACCCGAAAG
  GCTGGATACCTTAATGCTAGGAATAAAACAGGCTTGGTGTCATCTACCTGGGACAGACAG
  TTTTACTTCACGCAGGGTGGAAATTTAATGAGTCAGGCCCGTGGGGATGTAGCAGGAGGC
  CTGGCCATGGACATAGACAACTGTTCAGTGATGGCTGTGGACTGTGAAGACAGACGATAT
  TGTTTTCAGATCACCTCTTTCGATGGAAAAAAATCTTCAATTTTGCAAGCAGAGAGTAAA
  AAAGATCATGAAGAGTGGATCTGTACAATAAACAACATATCTAAACAAATATACTTAAGT
  GAAAATCCAGAGGAAACTGCTGCACGAGTAAATCAATCAGCTCTGGAAGCTGTCACTCCT
  TCCCCATCTTTCCAGCAGAGGCACGAGAGCCTGCGGCCAGCAGCAGGACAATCTCGGCCA
  CCGACAGCTCGAACCAGCAGTTCAGGATCCTTAGGATCTGAGTCTACAAATTTGGCTGCC
  CTCTCTCTAGATTCTCTTGTTGCCCCAGACACCCCAATACAGTTTGACATAATTTCTCCT
  GTGTGTGAAGATCAGCCTGGCCAGGCAAAAGCCTTTGGCCAGGGAGGCAGGCGTACAAAT
  CCATTTGGAGAATCTGGAGGAAGTACAAAATCTGAAACTGAAGATTCTATTCTTCATCAG
  TTATTTATTGTCCGATTCCTTGGTTCAATGGAGGTGAAATCAGATGACCATCCAGATGTT
  GTTTATGAAACTATGCGCCAAATCTTAGCTGCCCGGGCCATCCATAACATCTTTCGTATG
  ACAGAATCGCATTTATTAGTCACTTGTGACTGTTTAAAGTTAATTGATCCACAGACACAA
  GTTACAAGGCTCACGTTTCCATTACCTTGTGTAGTTTTGTATGCTACACACCAGGAAAAT
  AAGCGCCTTTTTGGATTTGTTCTTCGGACATCAAGCGGGAGAAGTGAAAGTAATCTGTCA
  TCAGTCTGCTATATATTTGAGTCAAACAATGAGGGGGAAAAGATATGTGATTCTGTTGGA
  CTGGCAAAACAGATAGCTTTGCATGCTGAACTGGATCGTAGGGCATCAGAAAAACAAAAA
  GAAATAGAGAGAGTAAAAGAGAAGCAACAGAAAGAACTCAATAAACAAAAACAGATTGAA
  AAGGACTTGGAAGAACAAAGTCGGTTGATAGCTGCTTCCAGTAGACCAAACCAAGCCAGT
  AGTGAGGGGCAGTTTGTTGTCCTTAGCAGTAGCCAGTCAGAAGAGAGTGATTTGGGAGAA
  GGAGGAAAGAAGAGAGAATCAGAAGCATAA
  
  Clone variation with respect to NM_012096.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012096 unedited
  TCGGAATTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCTGGNAGA
  AGCTGTGCGGGCGGGGACGGCTGCAGCCCTTGCCGGAGAGGGCGGGCCGGGGTCAGCTGC
  GGCGGGCGGGCCGGCGCGGGGAGCTGTGGGCGGCAGCTGCGTCTCCTGCCACCGCCCTCC
  CTCCGCCACGATGCCGGGGATCGACAAGCTGCCCATCGAGGAGACGCTGGAGGACAGCCC
  GCAGACAAGGTCTTTACTAGGTGTATTTGAAGAAGATGCCACAGCTATTTCCAACTATAT
  GAACCAGTTGTATCAAGCTATGCATCGGATTTATGATGCACAGAATGAATTAAGTGCAGC
  AACACACCTGACCTCAAAACTTTTAAAAGAATATGAAAAACAGCGTTTTCCATTGGGAGG
  TGATGATGAAGTTATGAGCTCTACATTGCAACAGTTTTCAAAAGTTATAGATGAGCTTAG
  CTCTTGTCATGCAGTGCTTTCAACTCAACTTGCTGATGCCATGATGTTCCCCATTACCCA
  GTTTAAAGAAAGAGATCTGAAAGAAATACTAACATTAAAGGAAGTATTTCAGATTGCAAG
  TAATGATCATGATGCTGCGATTAATAGATATAGCCGTTTATCAAAAAAAAGAGAAAATGA
  CAAGGGTGAAGTATGAAGTAACAGAAAGATGTGTACACATCCAGAAAGAAACAACACCAG
  ACCATGATGCATTATTTTTGTGCATTANATACTCTTCAGTACAAGAAGAANATAGCATTG
  TTAGAACCTCTACTTGNGTACATGNCAGCTCAGATAAGTTTCTTAAGAATGGGGTTCTGA
  AAATCTTAAATGACACTGGNAGAATTTTTAGCTAATATGGAACAGCGTTCAAATGGTCGC
  AGGAATGGACAGTGANTAGAGACATGCACAGACATAAGTTTTTGGAGTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012096 unedited
  GCTATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTTTATT
  CAGGGGGCAGGTTTTACAACATACTAGCTCTTAGGATCTCTCAGCATTAATAGGATTCAA
  ATTTTAAATAATATGGTTCCTAGATATAATGCAAAATTAACTCAAATGCCTTTACCATAT
  ACGATTTTATCTTCCCTAGGATGCACATCCTACTGACCTTTTCCCAAGTATAGTGGAAAC
  ACATTTACCACCCCTAGATTGTAGAGCCTGAGGATTCTTTTGAAATTAAACAATAATCAT
  TGCAGATGTGGCAAAAACTAGACTAGCTTTAGGGACTCTGTTCAAGATAATAGCTTTAGT
  CCCTGGTTGGCAAGTTCTCAAAATTTTTAAAGGTAGAAGGAATAACTGGTTTCCTATCTA
  CTGACTTCATTGCCATAGTTACAAACATACTGTTCAAATAGGTAACAGAATGCATTGTAG
  AAGTAGGCAGGCTTAATATCTTTTCTTTTTAAAAAGGAGTTTTGTTGATTTCTAACACCA
  TTAAGTGTAAGTCTGAATGTGAGAAAAGATATCCCAAAATCGAATCCAGTTACCCTCAAA
  GCATCTCAGAACTAGAAGGGATTTCAAAGGAGGGTAACAATCAGCTTTACCACTAGGCAG
  AAACCCATGGGCAGCCTTTTAATAACNAAAAAGAGCTTATTAAAACAGATACTTAAATCT
  CTGCAGTAATGGAATAGTTCATATAGTTCAACAGTAATGGAAACAAAAGCTGATCATCTA
  ANATTGNTAAACCCACTCATAACTTCTATTTAGCATTGNAATGCACTTCGATCTGACCTA
  TGGAGATGAGGGTTTATTTATGTAGAAATNCTGTGAACATGACTTAAACGAACTACAGGT
  CTGTCAGCATCCCTAGTCTGCCAGNGCCTAACTTTANCTACCTTGACCCAGGTGCTGATA
  AGGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012096
• Insert Size: 5270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012096.1, NP_036228.1
• RefSeq Size: 5970 bp
• RefSeq ORF: 2130 bp
• Locus ID: 26060
• UniProt ID: Q9UKG1
• Cytogenetics: 3p14.3
• Domains: PH, PID
• Protein Pathways: Colorectal cancer, Pathways in cancer
• Gene Summary: The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]