XRN2 (NM_012255) Human Untagged Clone

CAT#: SC115463

XRN2 (untagged)-Human 5'-3' exoribonuclease 2 (XRN2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XRN2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012255, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGTCCCGGCGTTCTTCCGCTGGCTCAGCCGCAAGTACCCGTCCATCATAGTCAACTGCGTGGAAG
  AGAAGCCAAAAGAATGCAATGGTGTAAAGATTCCAGTTGATGCCAGTAAACCTAATCCAAATGATGTGGA
  GTTTGATAATCTGTATTTGGATATGAATGGAATCATCCATCCCTGTACTCATCCTGAAGACAAACCAGCA
  CCAAAAAATGAAGATGAAATGATGGTTGCAATTTTTGAGTACATTGACAGACTTTTCAGTATTGTAAGAC
  CAAGAAGACTTCTCTACATGGCAATAGATGGAGTGGCACCACGTGCTAAAATGAACCAGCAGCGTTCAAG
  GAGGTTCAGGGCATCAAAAGAAGGAATGGAAGCAGCAGTCGAGAAGCAGCGAGTCAGGGAAGAAATATTG
  GCAAAAGGTGGCTTTCTTCCTCCAGAAGAAATAAAAGAAAGATTTGACAGCAACTGTATTACACCAGGAA
  CTGAATTCATGGACAATCTTGCTAAATGCCTTCGCTATTACATAGCTGATCGTTTAAATAATGACCCTGG
  GTGGAAAAATTTGACAGTTATTTTATCTGATGCTAGTGCTCCTGGTGAAGGAGAACATAAAATCATGGAT
  TACATTAGAAGGCAAAGAGCCCAGCCTAACCATGACCCAAATACTCATCATTGTTTATGTGGAGCAGATG
  CTGATCTCATTATGCTTGGCCTTGCCACACATGAACCGAACTTTACCATTATTAGAGAAGAATTCAAACC
  AAACAAGCCCAAACCATGTGGTCTTTGTAATCAGTTTGGACATGAGGTCAAAGATTGTGAAGGTTTGCCA
  AGAGAAAAGAAGGGAAAGCATGATGAACTTGCCGATAGTCTTCCTTGTGCAGAAGGAGAGTTTATCTTCC
  TTCGGCTTAATGTTCTTCGTGAGTATTTGGAAAGAGAACTCACAATGGCCAGCCTACCATTCACATTTGA
  TGTTGAGAGGAGCATTGATGACTGGGTTTTCATGTGCTTCTTTGTGGGAAATGACTTCCTCCCTCATTTG
  CCATCGTTAGAGATTAGGGAAAATGCAATTGACCGTTTGGTTAACATATACAAAAATGTGGTACACAAAA
  CTGGGGGTTACCTTACAGAAAGTGGTTATGTCAATCTGCAAAGAGTACAGATGATCATGTTAGCAGTTGG
  TGAAGTTGAGGATAGCATTTTTAAAAAGAGAAAGGATGATGAGGACAGTTTTAGAAGACGACAGAAAGAA
  AAAAGAAAGAGAATGAAGAGAGATCAACCAGCTTTCACTCCTAGTGGAATATTAACTCCTCATGCCTTGG
  GTTCAAGAAATTCACCAGGTTCTCAAGTAGCCAGTAATCCGAGACAAGCAGCCTATGAAATGAGGATGCA
  GAATAACTCTAGTCCTTCGATATCTCCTAATACGAGTTTCACATCTGATGGCTCCCCGTCTCCATTAGGA
  GGAATTAAGCGAAAAGCAGAAGACAGTGACAGTGAACCTGAGCCAGAGGATAATGTCAGGTTATGGGAAG
  CTGGCTGGAAGCAGCGGTACTACAAGAACAAATTTGATGTGGATGCAGCTGATGAGAAATTCCGTCGGAA
  AGTTGTGCAGTCGTACGTTGAAGGACTTTGCTGGGTTCTTAGATATTATTACCAGGGCTGTGCTTCCTGG
  AAGTGGTATTATCCATTTCATTATGCACCATTTGCTTCAGACTTTGAAGGCATTGCAGACATGCCATCTG
  ATTTTGAGAAGGGTACGAAACCGTTTAAACCACTAGAACAACTTATGGGGGTATTTCCAGCTGCAAGTGG
  TAATTTTCTACCTCCATCATGGCGGAAGCTCATGAGTGATCCTGATTCTAGTATAATTGACTTCTATCCT
  GAAGATTTTGCTATTGATTTGAATGGGAAGAAATATGCATGGCAAGGTGTTGCTCTCTTGCCATTCGTGG
  ATGAGCGAAGGCTACGAGCTGCCCTAGAAGAGGTATACCCAGACCTCACTCCAGAAGAGACCAGAAGAAA
  CAGCCTTGGAGGTGATGTCTTATTTGTGGGGAAACATCACCCACTCCATGACTTCATTTTAGAGCTGTAC
  CAGACAGGTTCCACAGAGCCAGTGGAGGTACCCCCTGAACTATGTCATGGGATTCAAGGAAAGTTTTCTT
  TGGATGAAGAAGCCATTCTTCCAGATCAAATAGTATGTTCTCCTGTTCCTATGTTAAGGGATCTGACACA
  GAACACTGTAGTCAGTATTAATTTTAAAGACCCACAGTTTGCTGAAGATTACATTTTTAAAGCTGTAATG
  CTTCCAGGAGCAAGAAAGCCAGCAGCAGTACTGAAACCTAGTGACTGGGAAAAATCCAGCAATGGACGGC
  AGTGGAAGCCTCAGCTTGGCTTTAACCGTGACCGGAGGCCTGTGCACCTGGATCAGGCAGCCTTCAGGAC
  TTTGGGCCATGTGATGCCAAGAGGCTCAGGAACTGGCATTTACAGCAATGCTGCACCACCACCTGTGACT
  TACCAGGGAAACTTATACAGGCCGCTTTTGAGAGGACAAGCCCAGATTCCAAAACTTATGTCAAATATGA
  GGCCCCAGGATTCCTGGCGAGGTCCTCCTCCCCTTTTCCAGCAGCAAAGGTTTGACAGAGGCGTTGGGGC
  TGAACCTCTGCTCCCATGGAACCGGATGCTGCAAACCCAGAATGCAGCCTTCCAGCCAAACCAGTACCAG
  ATGCTAGCTGGGCCTGGTGGGTATCCACCCAGACGAGATGATCGTGGAGGGAGACAGGGATATCCCAGAG
  AAGGAAGGAAATACCCTTTGCCACCACCCTCAGGAAGATACAATTGGAATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012255 unedited
  CACAGCAATTCGCACGAGGGTCAGCCGGTCGGCCGCCGCCTCCAGCCGTGTGCCGCTATG
  GGAGTCCCGGCGTTCTTCCGCTGGCTCAGCCGCAAGTACCCGTCCATCATAGTCAACTGC
  GTGGAAGAGAAGCCAAAAGAATGCAATGGTGTAAAGATTCCAGTTGATGCCAGTAAACCT
  ATTCCAAATGATGTGGAGTTTGATAATCTGTATTTGGATATGAATGGAATCATCCATCCC
  TGTACTCATCCTGAAGACAAACCAGCACCAAAAAATGAAGATGAAATGATGGTTGCAATT
  TTTGAGTACATTGACAGACTTTTCAGTATTGTAAGACCAAGAAGACTTCTCTACATGGCA
  ATAGATGGAGTGGCACCACGTGCTAAAATGAACCAGCAGCGTTCAAGGAGGTTCAGGGCA
  TCAAAAGAAGGAATGGAAGCAGCAGTCGAGAAGCAGCGAGTCAGGGAAGAAATATTGGCA
  AAAGGTGGCTTTCTTCCTCCAGAAGAAATAAAAGAAAGATTTGACAGCAACTGTATTACA
  CCAGGAACTGAATTCATGGACAATCTTGCTAAATGCCTTCGCTATTACATAGCTGATCGT
  TTAAATAATGACCCTGGGTGGAAAAATTTGACAGTTATTTTATCTGATGCTAGTGCTCCT
  GGTGAAGGAGAACATAAAATCATGGATTACATTAGAAGGCANAGAGCCCAGCCTAACCAT
  GACCCANATACTCATCATTGTTTATGTGGAGCAGATGCTGATCTCATTATGCTTGGGCCT
  GCCACACATGAACCGAACTTTACCATTATTAGAGAAGAATTCANACCANNACAGCCCAAA
  CCATGTGGGTCTTTGTAATCAGTTTGGACATGANGTCAAAGATGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012255 unedited
  GACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAGTTTTAAAAGTTTA
  ACTTTATTTTTTTTTACTAAAGTTCAGAAATTTCTGTAAGACAAGTACATTAATGAAATG
  TTTCCAAAGAAATACTGAACAATATATACTCTAGTTTGCTGAGGTTCCAGCTCAAGAGTT
  CAAACCTAATTCTTGTGCAATAAAAATCAGCATGGATCTTAGATGATCTAGAATACACTG
  TGTTTTGAAATCCACAGCTGGTTTCATTTTTAACCATTATGAAAAACCAGTACTCCTATT
  CCATCAAATGTGTTTTATAAGCAATAATAAATTCAGATCCACTGTATTATGCAACATACA
  TCTTTGGAAAGCAACATAAACAGTGAGATCAGATCAGTAGAAATATACACAGTTAAAAGA
  AATACACAAAGTACTGTAGTTTTATTAAAAACTACTACTTGAGAAAGAAATCTTTCCACA
  AATAGCATAAAACTGTAGAATGATGAAAGGATTTGGGAAAGCTTTACAAAAGCTTAATTC
  CAATTGTATCTTCCTGAGGGTGGTGGCAAAGGGTATTTCCTTCCTTCTCTGGGATATCCC
  TGTCTCCCTCCACGATCATCTCGTCTGGGTGGATACCCACCAGGCCCAGCTAGCATCTGG
  TACTGGTTTGGCTGGAAGGCTGCATTCTGGGTTTGCAGCATCCGGTTCCATGGGAGCAGA
  GGTNCAGCCCCAACGCCTCTGTCAAACCTTTGCTGCTGGAAAGGGGAGGAGGACCTCGCC
  AGGAATCCTGGGGCCTATATTTGACATAAGTTTGGATCTGGGCTTGTCTCTCAAAACGCC
  TGTTTAAGTTCCCTGGTAGTCACAGGGGGGGTGCAGCATGCTGTAAATGCATTCCTGACC
  CTTTGGCTCCCATGCCCAAATCCTGAAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012255
• Insert Size: 3570 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012255.3, NP_036387.2
• RefSeq Size: 3449 bp
• RefSeq ORF: 2853 bp
• Locus ID: 22803
• UniProt ID: Q9H0D6
• Cytogenetics: 20p11.22
• Domains: DUF251
• Protein Pathways: RNA degradation
• Gene Summary: This gene encodes a 5'-3' exonuclease that promotes transcription termination at cotranscriptional cleavage sites. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (2) contains an alternate 5' terminal exon, resulting in a distinct 5' UTR and the use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus , and is shorter, compared to isoform 1.