UCK2 (NM_012474) Human Untagged Clone

CAT#: SC115372

UCK2 (untagged)-Human uridine-cytidine kinase 2 (UCK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UCK2
• Synonyms: TSA903; UK; UMPK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115372 sequence for NM_012474 edited (data generated by NextGen Sequencing)
  ATGGCCGGGGACAGCGAGCAGACCCTGCAGAACCACCAGCAGCCCAACGGCGGCGAGCCC
  TTCCTTATAGGCGTCAGCGGGGGAACAGCTAGCGGCAAGTCTTCCGTGTGTGCTAAGATC
  GTGCAGCTCCTGGGGCAGAATGAGGTGGACTATCGCCAGAAGCAGGTGGTCATCCTGAGC
  CAGGATAGCTTCTACCGTGTCCTTACCTCGGAGCAGAAGGCCAAAGCCCTGAAGGGCCAG
  TTCAACTTTGACCACCCGGATGCCTTTGACAATGAACTCATTCTCAAAACACTCAAAGAA
  ATCACTGAAGGGAAAACAGTCCAGATCCCCGTGTATGACTTTGTCTCCCATTCCCGGAAG
  GAGGAGACAGTTACTGTCTATCCCGCAGACGTGGTGCTCTTTGAAGGGATCCTGGCCTTC
  TACTCCCAGGAGGTACGAGACCTGTTCCAGATGAAGCTTTTTGTGGATACAGATGCGGAC
  ACCCGGCTCTCACGCAGAGTATTAAGGGACATCAGCGAGAGAGGCAGGGATCTTGAGCAG
  ATTTTATCTCAGTACATTACGTTCGTCAAGCCTGCCTTTGAGGAATTCTGCTTGCCAACA
  AAGAAGTATGCTGATGTGATCATCCCTAGAGGTGCAGATAATCTGGTGGCCATCAACCTC
  ATCGTGCAGCACATCCAGGACATCCTGAATGGAGGGCCCTCCAAACGGCAGACCAATGGC
  TGTCTCAACGGCTACACCCCTTCACGCAAGAGGCAGGCATCGGAGTCCAGCAGCAGGCCG
  CATTGA
  
  Clone variation with respect to NM_012474.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012474 unedited
  CGGCCGGCGAATTCGCACGAGGGTCCGACGCGGGCGCGGGCGGGGAGCGTGCGTCCGTTC
  GCACAGGCAGCGGGAGGAGGGGCGGCGCGAACCATGGCCGGGGACAGCGAGCAGACCCTG
  CAGAACCACCAGCAGCCCAACGGCGGCGAGCCCTTCCTTATAGGCGTCAGCGGGGGAACA
  GCTAGCGGCAAGTCTTCCGTGTGTGCTAAGATCGTGCAGCTCCTGGGGCAGAATGAGGTG
  GACTATCGCCAGAAGCAGGTGGTCATCCTGAGCCAGGATAGCTTCTACCGTGTCCTTACC
  TCGGAGCAGAAGGCCAAAGCCCTGAAGGGCCAGTTCAACTTTGACCACCCGGATGCCTTT
  GACAATGAACTCATTCTCAAAACACTCAAAGAAATCACTGAAGGGAAAACAGTCCAGATC
  CCCGTGTATGACTTTGTCTCCCATTCCCGGAAGGAGGAGACAGTTACTGTCTATCCCGCA
  GACGTGGTGCTCTTTGAAGGGATCCTGGCCTTCTACTCCCAGGAGGTACGAGACCTGTTC
  CAGATGAAGCTTTTTGTGGATACAGATGCGGACACCCGGCTCTCACGCAGAGTATTAAGG
  GACATCAGCGAGAGAGGCAGGGGATCTTGAGCAGATTNTATCTCAGTACATTACGTTCGT
  CAAGCCTGCCTTTGAGGAATTCTGNCTGCCAACAAAGAGTATGCTGATGTGATCATCCCT
  AGAGTGCAGATAATCTGGTGGGCATCAACCTCATCGTGCAGCAAATCCAGGACATNCTGA
  ATGGGAGGGCCCTNCAACGGCAGACCATGGCTGTCTCACGGNNTACACCCCTTACGCAGA
  AGCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012474 unedited
  CCATAAGTGNACNCGCGGCCGCAATTTANGATCGAGTTTTTTTTTTTTTTTTTTGCCTTT
  TTGTCTGTCTTTATTTACAGCAAAGTACATAATATGTGGTCACTTTGTAACCACTAACAA
  TAAAGGTAGGGTGGTAAAAAGAAAAGGGAGAGGAGCAAGCATCTTAAAAGGGGGGAAAAA
  AAAGCCTAAGTCTGTATTTAGCTACTCACCCTGCTCCTGAGGCAGGACTGGGTAGGCACT
  CCCTGCCTCTAAGACCACAGCCAAGCTGACCTGGAATGCCAGTCCCCGGTATGAGGAAGG
  CAGCAGAACGCCCAGAGACAAAAGATTAAAGGGGACTGAGCTCTGGATGCATCCACTTAT
  GCTTTGCCAGACAGTTCCCTTCCTTGTTTTGTTTGAATGCTTCCCCCACCCCGCCCTGAG
  CAAAGTGTAAAGGGCAACGGTGGCACAGAAGTACAGGCAGTCCCTTCCAGAAGCAGTCCT
  GAACCGCATAACCCACCTACCTAGCTGCTCCTCAGACTGCCGAGTTCTTGGCCCTGGAAT
  CTTTAACAGGCACATAGGAGATTCCAGGGCAGGGGAAAATATTTGCCCTGCAAGAGGCTA
  CTTCCCCTCCCACCACCTGTGACGCCCACCCGCCCGCCAATGACTCCTCTCTCCACCGAA
  AGTGCCCTACTGCCTCACACAGGTTCTTATCATGTCTCATCCAACCTTCTTGCCTCTTCC
  TGCTGCCCCACTGCCCCCCAGGTGCTCCCCATTTCCCCCCCGCTTTCCCCAACCCCTCTC
  TTTGCTACTCGCTGCTATCCTACTCCTTGCGCGCCCGTCCCTCTTTCATCTCTACTCTCC
  TTCTCAATTTCCGGGCCCCTCCCTCCTATCTTCCTCCGTNTTCGCCATTGCTTCCTACAT
  CCATCCCATCCACCTCCCNTCTCCTATTCTTATCTCTGGTTCTGGTACACCCGCCCCCAA
  CCCTCCACACTACTCTATNTCCCCTGAACCCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012474
• Insert Size: 4860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012474.3, NP_036606.2
• RefSeq Size: 1284 bp
• RefSeq ORF: 786 bp
• Locus ID: 7371
• UniProt ID: Q9BZX2
• Cytogenetics: 1q24.1
• Domains: PRK
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
• Gene Summary: This gene encodes a pyrimidine ribonucleoside kinase. The encoded protein (EC 2.7.1.48) catalyzes phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively.[provided by RefSeq, Oct 2010]