ATG4B (NM_013325) Human Untagged Clone

CAT#: SC115281

ATG4B (untagged)-Human ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATG4B
• Synonyms: APG4B; AUTL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115281 sequence for NM_013325 edited (data generated by NextGen Sequencing)
  ATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCT
  GAGACCTCAGAGCCCGTTTGGATACTGGGTAGAAAATACAGCATTTTCACAGAAAAGGAC
  GAGATCTTGTCTGATGTGGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCCAGCC
  ATTGGGGGGACAGGCCCCACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAG
  ATGATCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACACAA
  AGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGAC
  AGTTACTACTCCATTCACCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTCCATAGGC
  CAGTGGTACGGGCCCAACACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACG
  TGGAGCTCCTTGGCGGTCCACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGA
  AGGTTGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCGAC
  CGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGA
  CCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGCTCACGGACATCAACGAGGCCTACGTG
  GAGACGCTGAAGCACTGCTTCATGATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCC
  AACAGCGCCCACTACTTCATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACCCCCAC
  ACCACGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGCTTCCAC
  TGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGAGCTTGACCCGTCCATCGCTGTGGGG
  TTTTTCTGTAAGACTGAAGATGACTTCAATGATTGGTGCCAGCAAGTCAAAAAGCTGTCT
  CTGCTTGGAGGTGCCCTGCCCATGTTTGAGCTGGTGGAGCAGCAGCCTTCACATCTGGCC
  TGCCCCGACGTCCTGAACCTGTCCCTAGATTCTTCTGATGTAGAGCGACTGGAAAGATTC
  TTCGACTCAGAAGATGAAGACTTTGAAATCCTGTCCCTTTGA
  
  Clone variation with respect to NM_013325.4
  1061 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013325 unedited
  CTATAGGGGCGGCCGCGAATTCGCACGAGGCCGGACTGGGAAGATGGACGCAGCTACTCT
  GACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCTGAGACCTCAGAGCCCGT
  TTGGATACTGGGTAGAAAATACAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGT
  GGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCCAGCCATTGGGGGGACAGGCCC
  CACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAGATGATCTTTGCCCAAGC
  CCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACACAAAGGAAGAGGCAGCCAGA
  CAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGACAGTTACTACTCCATTCA
  CCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCCAA
  CACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGCGGT
  CCACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGAAGGTTGTGCAGGACCAG
  CGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCGACCGGCACTGCAACGGATT
  CCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGACCCCTGGTACTTCTCAT
  TCCCCTGCGCCTGNNGCTCACGGACATCAACGAGGCCTACGTGGAGACGCTGAAGCACTG
  CTTCATGATGCCCNCAGTCCTGGGGCGTCATCGGGAGGGAAGCCCNACAGCGCCACTACT
  TCATCGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013325 unedited
  CCCTGAAGGGTNATTTATCTCCTTACAACCACANAACCGGCGCCTGGGAGCAGTGGGACT
  GAGGCCCAGGTGCTAACACGGGGCTGGCAGTGTCGAGAGAACGCTCTGGAAGCTCCTAAC
  AGACGGCTCCGCGTGCGGATGCACAGGCCCTGACGGGCACTCTGAGCTGGGCAGTCTGAC
  ACCAAGCAGTAAGGCCTCCCGGGCAGCGCAGCCTCAGTCCACGAGCACAGCGGGTGGCCC
  TCTGGGGGGAGGCAGCACGGGGCGCAGCCCTCGGCAGGCGAGCGGGCGGGATGGATGAAA
  CGCAGCGGCACCAGGAGCCCCAGGCTCTCACAGGTGCCACCCCCGACCCCAGGATTTTCA
  AAGGGACAGGATTTCAAAGTCTTCATCTTCTGAGTCGAAGAATCTTTCCAGTCGCTCTAC
  ATCAGAAGAATCTAGGGACAGGTTCAGGACGTCGGGGCAGGCCAGATGTGAAGGCTGCTG
  CTCCACCAGCTCAAACATGGGCAGGGCACCTCCAAGCAGAGACAGCTTTTTGACTTGCTG
  GCACCAATCATTGAAGTCATCTTCAGTCTTACAGAAAAACCCCACAGCGATGGACGGGTC
  AAGCTCCGCGATGCTCATGCGGCACGGCGGGTGCTGGCAGTGGAAGCTCTCGTCCGGGAT
  GAAGCAGCCATCAGTGGGCTCCACGGCTGGCTGCGTGGTGTGGGGGTCCAGGTAGATGAG
  CTCCTCACCAACGTAGCCGATGAAGTAGTGGGCGCTGTTGGGCTTCCCTCCGATGACGCC
  CAGGGACTGGGGCATCATGAAGCAGTGCTTCAGCGNTCTCACGTAGGCCTCGTTGATGTC
  CGTGAGCCCCAGGCGCATGGGAATGAGAAGTACCAGGGGTCTCCATGGCGACAGCCTGTT
  GGTGACCTCAGCTCCGGCAAGGAATCCGTTGCAATGCCGGTCGGATCTGCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013325
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013325.4, NP_037457.3
• RefSeq Size: 2892 bp
• RefSeq ORF: 1182 bp
• Locus ID: 23192
• UniProt ID: Q9Y4P1
• Cytogenetics: 2q37.3
• Domains: Peptidase_C54
• Protein Families: Protease
• Protein Pathways: Regulation of autophagy
• Gene Summary: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) is the predominant transcript and it encodes the longer isoform (a).