ALIX (PDCD6IP) (NM_013374) Human Untagged Clone

SKU SC115270

PDCD6IP (untagged)-Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ALIX
• Synonyms: AIP1; ALIX; DRIP4; HP95
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115270 sequence for NM_013374 edited (data generated by NextGen Sequencing); ATGGCGACATTCATCTCGGTGCAGCTGAAAAAGACCTCAGAGGTGGACCTGGCCAAGCCG; CTGGTGAAGTTCATCCAGCAGACTTACCCAAGCGGCGGGGAAGAGCAGGCCCAGTACTGC; CGCGCGGCGGAGGAGCTCAGCAAGCTGCGCCGCGCCGCAGTCGGTCGTCCGCTGGACAAG; CACGAGGGCGCGCTCGAGACGCTCCTGAGATATTATGATCAGATTTGTTCTATTGAACCC; AAATTCCCATTTTCTGAAAATCAGATCTGCTTGACATTTACCTGGAAGGATGCTTTCGAT; AAAGGTTCACTTTTTGGAGGCTCTGTAAAACTGGCTCTTGCAAGCTTAGGATATGAAAAG; AGCTGTGTGTTGTTCAATTGTGCAGCCTTAGCTAGCCAAATTGCAGCAGAACAGAACCTG; GATAATGATGAAGGATTGAAAATCGCTGCTAAACATTACCAGTTTGCTAGTGGTGCCTTT; TTACATATTAAAGAGACGGTTTTATCTGCCTTAAGTCGAGAGCCGACCGTGGACATATCT; CCAGATACTGTTGGGACCCTCAGTCTTATTATGCTGGCACAGGCTCAAGAAGTATTTTTT; TTAAAAGCCACAAGAGATAAAATGAAAGATGCCATCATAGCTAAATTGGCTAATCAGGCT; GCAGATTATTTTGGTGATGCTTTCAAACAGTGTCAATACAAAGATACTCTCCCCAAGGAG; GTGTTCCCTGTCTTGGCTGCAAAGCACTGTATCATGCAGGCCAATGCTGAGTACCATCAG; TCTATCCTGGCAAAACAGCAGAAGAAATTTGGAGAAGAAATTGCAAGGTTACAGCATGCA; GCAGAACTGATTAAAACAGTGGCATCTCGCTATGATGAATATGTTAATGTGAAGGATTTT; TCTGACAAAATCAATCGTGCCCTTGCTGCAGCAAAGAAGGATAATGACTTCATTTATCAT; GATCGAGTTCCAGACCTTAAAGATCTAGATCCTATTGGCAAAGCCACACTTGTGAAATCT; ACCCCGGTCAATGTACCCATCAGTCAGAAATTTACTGATCTGTTTGAGAAGATGGTTCCC; GTGTCAGTACAGCAGTCTTTGGCTGCCTATAATCAGAGGAAAGCCGATTTGATTAACAGA; TCAATTGCTCAGATGAGAGAAGCCACCACTTTGGCAAATGGGGTGCTAGCTTCCCTTAAT; CTTCCAGCAGCAATTGAAGATGTGTCTGGAGACACTGTACCTCAGTCTATATTGACTAAA; TCCAGATCTGTGATTGAACAGGGAGGCATCCAGACTGTTGATCAGTTGATTAAAGAACTG; CCTGAATTACTGCAACGAAATAGAGAAATCCTAGATGAGTCATTAAGGTTGTTGGATGAA; GAAGAAGCAACCGATAATGATTTAAGAGCAAAATTTAAGGAACGTTGGCAAAGGACACCA; TCCAATGAACTGTATAAGCCTTTAAGAGCAGAGGGAACCAACTTCAGAACAGTTTTAGAT; AAAGCTGTGCAGGCAGATGGACAAGTGAAAGAATGTTACCAGTCTCATCGTGACACCATC; GTGCTTTTGTGTAAGCCAGAGCCTGAGCTGAATGCTGCCATCCCTTCTGCTAATCCAGCA; AAGACCATGCAGGGCAGTGAGGTTGTAAGTGTCTTAAAATCCTTATTGTCAAATCTTGAT; GAAGTAAAGAAGGAAAGAGAGGGTCTGGAGAATGACTTGAAATCTGTGAATTTTGACATG; ACAAGCAAGTTTTTGACAGCCCTGGCTCAAGATGGTGTGATAAATGAAGAAGCTCTTTCT; GTTACTGAACTAGATCGAGTCTATGGAGGTCTTACAACTAAAGTCCAAGAATCTCTAAAG; AAACAGGAGGGACTTCTTAAAAATATTCAGGTCTCACATCAGGAATTTTCAAAAATGAAA; CAATCTAATAATGAAGCTAACTTAAGAGAAGAAGTTTTGAAGAATTTAGCTACTGCATAT; GACAACTTTGTTGAACTTGTAGCTAATTTGAAGGAAGGCACAAAGTTTTACAATGAGTTG; ACTGAAATCCTGGTCAGGTTCCAGAACAAATGCAGTGATATAGTTTTTGCACGGAAGACA; GAAAGAGATGAACTCTTAAAGGACTTGCAACAAAGCATTGCCAGAGAACCTAGTGCTCCT; TCAATTCCTACACCTGCGTATCAGTCCTCACCAGCAGGAGGACATGCACCAACTCCTCCA; ACTCCAGCGCCAAGAACCATGCCGCCTACTAAGCCCCAGCCCCCAGCCAGGCCTCCACCA; CCTGTGCTTCCAGCAAATCGAGCTCCTTCTGCTACTGCTCCATCTCCAGTGGGGGCTGGG; ACTGCTGCGCCAGCTCCATCACAAACGCCTGGCTCAGCTCCTCCTCCACAGGCGCAGGGA; CCACCCTATCCCACCTATCCAGGATATCCTGGGTATTGCCAAATGCCCATGCCCATGGGC; TATAATCCTTATGCGTATGGCCAGTATAATATGCCATATCCACCAGTGTATCACCAGAGT; CCTGGACAGGCTCCGTACCCGGGACCCCAGCAGCCTTCATACCCCTTCCCTCAGCCCCCA; CAGCAGTCTTACTATCCACAGCAGTAA; ; Clone variation with respect to NM_013374.4; 1132 g=>a;1649 a=>g;2535 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013374 unedited; GTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGTCAGCCA; GTCAGTCCGCCAGTCCGCCAGCCCAGTACCTCTCTCTCCTCGGCCCTCGTAAGCTGTCCG; CGGTCTGTTTGGCCCGAACGGCGGCGGAGGCGCTGATCATGGCGACATTCATCTCGGTGC; AGNCTGAAAAAGACCTCAGAGGTGGACCTGGCCAAGCCGCTGGTGAAGTTCATCCAGCAG; ACTTACCCAAGCGGCGGGGAAGAGCAGGCCCAGTACTGCCGCGCGGCGGAGGAGCTCAGC; AAGCTGCGCCGCGCCGCAGTCGGTCGTCCGCTGGACAAGCACGAGGGCGCGCTCGAGACG; CTCCTGAGATATTATGATCAGATTTGTTCTATTGAACCCAAATTCCCATTTTCTGAAAAT; CAGATCTGCTTGACATTTACCTGGAAGGATGCTTTCGATAAAGGTTCACTTTTTGGAGGC; TCTGTAAAACTGGCTCTTGCAAGCTTAGGATATGAAAAGAGCTGTGTGTTGTTCAATTGT; GCAGCCTTAGCTAGCCAAATTGCAGCAGAACAGAACCTGNATAATGATGAAGGATTGAAA; ATCGCTGCTAAACATTACCAGTTTGCTAGTGGTGCCTTTTTACATATTAAAGAGACGGTT; NTATCTGCCTTAAGTCGAGAGCCGACCGTGGACATATCTCCAGATACTGTTGGGACCCTC; AGTCTTATTATGCTGGCACAGGCTCAAGAAGTATTTTTTTTTAAAGCCACAAGAGATAAA; TGAAAGATGCCATCATAGCTAAATGGNCTAATCANGCTGCAGATATTTTGGTGATGCCTT; CAAACAGTGTCAATACAAAGATACTCTCCCCAAGGNNAGTGTCCCTGTCTTGGCTGCAAG; CACTGTATCATGCAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013374 unedited; TGGACGCGGCCGCAATCTAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTAACGGGTCTTAAGAAATTTATTTTAAAATTATAAAATTTACAGGGCCTTGATT; ATGCAAAATAGCATAATGGAAATTAAACCAAATCAATAAACCAAAGAGAAAGAAAACTTA; ATTTTCTTTAGTATCCATACTTAAACCATTTTTGTAAGTATCTGATGTCCCAACCATGTT; TTATGTAAAAAGTATAATCGTTTCAAAGGTTTCACTTGCAGGTTAAATTTCTCATTTTCA; ATTTTTATGAACTGTAATGCAATTTCAAATCCTATTATACCTAGGGTTTATACTGCAACA; GCAGCAAATCTCACATGGGTAATCAAATGGGGAACTGGGGCACAGCTTCTAGCTGTAAAC; AGAAATTATACACTGCATTCAGTCCAGGAGAGTACATTACATTAACCAGAGCGTAGAGTT; TAGTACACTTATTGCAGGGCGGGTATTTCTTTCCCTCTGATCTGAATCAGCTGAGCTGGT; GAGCAGACATATTACTGCTGGGGATAGTAAAACTGCTGTGGGGGCTGAGGGAAGGGGTAT; GAAGGCTGCTGGGGTCCCGGGTATGGAGCCCTGTCCAGCACTCTGGAGATACACTGGGGG; ATATGGAATATTATACTGCCCATACGCATAAGGATTATAGCCCCATGGGCCATGGCGATT; TGGAAATACCCAGGATATTCTGAAAAGGTGGGAAAAGGGTGGCCCCTGCCCCTGTGGAGG; AGGAGCTGACCACGCCGTTGAGAGGAACTTGCGCCACATTCCCAGCCCCCATGTAGATGG; AACATAACAAAAGGACCTCGATTTGCTGGAAACACAGGGGTGGAGCCTGGCTGGGGCTGG; GCTTAAAAGCGGATGGCCTTGTGCCCGAATTGGAGGAATTGCGCATGTCCTCTGCGGAGA; GACTGAACCCAGTGTAGGATAGAAG;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_013374
• Insert Size: 3330 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013374.3, NP_037506.2
• RefSeq Size: 6448 bp
• RefSeq ORF: 2607 bp
• Locus ID: 10015
• UniProt ID: Q8WUM4
• Cytogenetics: 3p22.3
• Domains: BRO1
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Summary: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]; Transcript Variant: This variant (1) uses an alternate in-frame splice site in the central coding region, compared to variant 2, resulting in an isoform (1) that is shorter than isoform 2.