HIGD1A (NM_014056) Human Untagged Clone

CAT#: SC115198

HIGD1A (untagged)-Human HIG1 hypoxia inducible domain family, member 1A (HIGD1A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIGD1A
• Synonyms: HIG1; RCF1a
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115198 sequence for NM_014056 edited (data generated by NextGen Sequencing)
  ATGTCAACAGACACAGGTGTTTCCCTTCCTTCATATGAGGAAGATCAGGGATCAAAACTC
  ATTCGAAAAGCTAAAGAGGCACCATTCGTACCCGTTGGAATAGCGGGTTTTGCAGCAATT
  GTTGCATATGGATTATATAAACTGAAGAGCAGGGGAAATACTAAAATGTCCATTCATCTG
  ATCCACATGCGTGTGGCAGCCCAAGGCTTTGTTGTAGGAGCAATGACTGTTGGTATGGGC
  TATTCCATGTATCGGGAATTCTGGGCAAAACCTAAGCCTTAG
  
  Clone variation with respect to NM_014056.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014056 unedited
  TAGAGTCGACTATTGTAAACGACTCACAAAGGCGGCCGCGACATCGGCACGAGNAGAGGT
  TTTCTCGCTCTAGGGAGATTCTTCAAGCAATCACTATGTCAACAGACACAGGTGTTTCCC
  TTCCTTCATATGAGGAAGATCAGGGATCAAAACTCATTCGAAAAGCTAAAGAGGCACCAT
  TCGTACCCGTTGGAATAGCGGGTTTTGCAGCAATTGTTGCATATGGATTATATAAACTGA
  AGAGCAGGGGAAATACTAAAATGTCCATTCATCTGATCCACATGCGTGTGGCAGCCCAAG
  GCTTTGTTGTAGGAGCAATGACTGTTGGTATGGGCTATTCCATGTATCGGGAATTCTGGG
  CAAAACCTAAGCCTTAGAAGAAGAGATGCTGTCTTGGTCTTGTTGGAGGAGCTTGCTTTA
  GTTAGATGTCTTATTATTAAAGTTACCTATTATTGTTGGAAATAAACTAATTTGTATGGG
  TTTAGATGGTAACATGGCATTTTGAATATTGGCTTCCTTTCTTGCAGGCTTGATTTGCTT
  GGTGACCGAATTACTAGTGACTAGTTTACTAACTAGGTCATTCAAGGAAGTCAAGTTAAC
  TTAAACATGTCACCTAAATGCACTTGATGGTGTTGAAATGTCCACCTTCTTAAATTTTTA
  AGATGAACTTAGTTCTAAAGAAGATAACAGGCCAATCCTGAAGGTACTCCCTGTTTGCTG
  CAGAATGTCAGATATTTTTGGATGTTGCATAAGAGTCCTATTTGCCCCAGTTAATTCAAC
  TTTTGTCTGCCTGTTTTGTGGACTGGCTGGCTCTGTTAGAACTCTGTCCAAAAGTGCATG
  GAATATAAACTTGTAAGCTTCCCACATTGACATATATATGCATGTGTTTAAACCAATCCA
  NGAAGCTTAAACATAGAGCTGCATAATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014056 unedited
  AATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGGTCACTTACTTTAATAACAATACATA
  TACCATGTTAACACCATGGAATGCAAATTCAGATTAGACAAGAGAATTTCACAAGTGTGA
  TAGCCTTCTGTATATTATATAAAAGTTTGGGTATACTGTCTGGCCAAACCAGCTTGCTCA
  TAAGTCATTAACCAAATCCATTATAGGTAATTTGTTCAGTTCAATGTTTACAATTCTTAT
  GGAAAAAATTAGCAACACACACATTTAAAACGTGTGCATTTACCTTTGCGTGAGTGCTTA
  AAATACATATTTCTATTTCAAGATGACATTTAAAAATTATTCTAATATATCAGCAGCAAA
  AATATAATTTGCAATTACAAAAAACTAAACTAGAATCCTTAAGTTATTCTCATGTTTACA
  GTTGTGATTCTTTAATAAATACTATTATGCAGCTCTATTGTTTAAGCTTTCTGGATTTGG
  TTTAAACACATGCATATATATTGTCAATTGTGGGAAGCTTTACAAGTTATATTCCATGCA
  CTTTTTGGACAGAGTTCTAACAGAGCCAGCCAGTCCACANAACAGGCAGACAAAAAGTGA
  ATTAACTNNGGGCAATANGACTCTTTATGCACATCCAAAATATCTGACATTCTGCAGCAA
  ACAGGGAGTACCTTCANNGATGGCCTGTTATCTCCTTTAGACTAAGNTCATCTTAAAATT
  TAAGAAGGTGACACTTCACACCATCAGTGCATTANGTGACATGTTTAAGTAACTTGACTT
  CCTTGATGACTAGTTAGAAACTAGTCCTAGTAATTCGCCACAGCAATCAACCTGCAGAAA
  GGAGCCAATTCAAATGCCTGTTACATCTAACCATCCAATATNTATTCCACATATAGTACT
  TATAATAGATTTACTAAGCAGTCTCCACAGACAGAAGATTTTTTTTTAGCTAGTTGCCCA
  ATCCAACTGAATCCTCCACGTATGTCTAACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014056
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014056.1, NP_054775.1
• RefSeq Size: 1362 bp
• RefSeq ORF: 282 bp
• Locus ID: 25994
• UniProt ID: Q9Y241
• Cytogenetics: 3p22.1
• Domains: HIG_1_N
• Protein Families: Transmembrane
• Gene Summary: Proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains a distinct 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and a protein (isoform b) with a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.