NIPP1 (PPP1R8) (NM_014110) Human Untagged Clone

CAT#: SC115156

PPP1R8 (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NIPP1
• Synonyms: ARD-1; ARD1; NIPP-1; NIPP1; PRO2047
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014110, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGACTGCCCAACCTGGGCAGGTAAGCCCC
  CTCCCGGTTTACATCTGGATGTAGTCAAAGGAGACAAACTAATTGAGAAACTGATTATTGATGAGAAGAA
  GTATTACTTATTTGGGAGAAACCCTGATTTGTGTGACTTTACCATTGACCACCAGTCTTGCTCTCGGGTC
  CATGCTGCACTTGTCTACCACAAGCATCTGAAGAGAGTTTTCCTGATAGATCTCAACAGTACACACGGCA
  CTTTCTTGGGTCACATTCGGTTGGAACCTCACAAGCCTCAGCAAATTCCCATCGATTCCACGGTCTCATT
  TGGCGCATCCACAAGGGCATACACTCTGCGCGAGAAGCCTCAGACATTGCCATCGGCTGTGAAAGGAGAT
  GAGAAGATGGGTGGAGAGGATGATGAACTCAAGGGCTTACTGGGGCTTCCAGAGGAGGAAACTGAGCTTG
  ATAACCTGACAGAGTTCAACACTGCCCACAACAAGCGGATTTCTACCCTTACCATTGAGGAGGGAAATCT
  GGACATTCAAAGACCAAAGAGGAAGAGGAAGAACTCACGGGTGACATTCAGTGAGGATGATGAGATCATC
  AACCCAGAGGATGTGGATCCCTCAGTTGGTCGATTCAGGAACATGGTGCAAACTGCAGTGGTCCCAGTCA
  AGAAGAAGCGTGTGGAGGGCCCTGGCTCCCTGGGCCTGGAGGAATCAGGGAGCAGGCGCATGCAGAACTT
  TGCCTTCAGCGGAGGACTCTACGGGGGCCTGCCCCCCACACACAGTGAAGCAGGCTCCCAGCCACATGGC
  ATCCATGGGACAGCACTCATCGGTGGCTTGCCCATGCCATACCCAAACCTTGCCCCTGATGTGGACTTGA
  CTCCTGTTGTGCCGTCAGCAGTGAACATGAACCCTGCACCAAACCCTGCAGTCTATAACCCTGAAGCTGT
  AAATGAACCCAAGAAGAAGAAATATGCAAAAGAGGCTTGGCCAGGCAAGAAGCCCACACCTTCCTTGCTG
  ATTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014110 unedited
  AACCTGGTGAGTGGCGGGGCGGCCAGGGCTAGAGTGGCCCGGCCGGAGCTAGCCTGGGCT
  GGAAGGGCGGCTCTTTTTTTACTTTTCTGCTGCGAGCCGAACGGCTCAGAAACCCCGGAA
  TGGTTGAGGAAAAACTGTTTGCTGCACCGGGCCGGGCGACGTGTTGAAGAACCGAGAGCC
  TGGAGCCCAGGCCCAGGAACTGAAGAAACCCGGGGTTGGGGGCTCAAAGGCGCTCACTTA
  GGCAGCCCCTTTGAGCGATTAGCCAGTCGCCGGAGCGCTTCGAGGCCTTGGCCCGAACTT
  ACGCCCAACTCTTGACTGAGTGCCTGGTGCTCTCGTGGAGCATCGCATCTGGCCCCTTCC
  TGTACGTCCCGAGCGCGCTCGAGCCAGCCCCGGCCCCAACCCTACCTCCAAGCCCCGCAT
  CCCTCTGTGGTTGCTGCATCCCTCGTGCGGCACTTGTCTGTCTGCCACAGAGAACACGAG
  GGGCAGGTAAGCCCCCTCCCGGTTTACATCTGGATGTAGTCAAAGGAGACAAACTAATTG
  AGAAACTGATTATTGATGAGAAGAAGTATTACTTATTTGGGAGAAACCCTGATTTGTGTG
  ACTTTACCATTGACCACCAGTCTTGCTCTCGGGTCCATGCTGCACTTGTCTACCACAAGC
  ATCTGAAGAGAGTTTTCCTGATAGATCTCACAGTAAACCTGACAGAGTTCACACTGCCCA
  CACAAGCGGATNTCTACCCTTACATTNGAGAGGNAATCTGGACATTCAAGACCAAGAGGA
  GAGGAGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014110 unedited
  TTAATCTACAATGTAATAAAGGTTACAGNGTAAAAAGCTACACATAAAGCATGAAAAGGC
  CTATTACACAAATGTACAAATCTCTGTACAAAGCTTGTATCAATGTTTCCTAACTTTGTC
  TCCTATGTTTGTTAGCCAGGTCTAGTCTTGGAGCTCTGAATAACAAGAGCTCTCAATTGA
  GGGGGGAAAGAGCCTCCAAACTGACATCTAAAAATTCCTCTTAGAGACACCAGTTACTTC
  CAAACAAATCTTTTCCTTTTTAATTTGAATTACCCCAACTGGTCATATCCTTTGAAAACT
  GCCTTCATAATACACTTAAAAGTAATGCCTTAGAGTAGAGGACAGTACTCTGGAATTAAA
  TAAGAAACTTCAGCAGGAGGAGTGCTCAGGTTTGACAAGAAAAACAGGTAAATTTCAAAC
  AATACTGTAAGTTAAAACTATAAACCTGTGTCTATGGCACCAGTCACAGATGAAGGAAAA
  TCGAAATGTTTTCATTCCAACAGGAAGGACAAAAGAGAAAAGCCTAGCTACTGGACCTCA
  AAACCACTGACCCAAGGAAACATTNTTTAGTTTAAACCTCAGCTTAACTGCAGAATTCTA
  AGCCTTCTTTTTGATCAAACTGAGATCTATGGCAACCCTTCTGCTACCCGGGACTTGGAA
  GGATAGTGAATATAAATTANGCTCTGGACCCCCAAGCTGGTGATGCTACTGTAACCTCTA
  CAGCTCTGAATTCACCTGAGGCCTGGGATATGTGACTTTACTTAAATTGATCCTAGAAAG
  TGGAGAAAGAATGAAGTATGGGACGCCTCAGAAGAGCCAGGGCCTCTTTTGAAAGGATTT
  AGAGCCTTTCTTTACATGCCAGGGTTGTGGAGACCGTCTTTATGGCTTTTTCACCCCACC
  CCAATGGCATTTACTTGGTTGGAGGCCCGAAACTTTTGAGAACTACCGGCCACTGGAAGT
  GTTCTTCCTATTAATAGATCCATAACCTTCCACCATTTCACCAATCCCCCCTTTCTGACA
  AAAATAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014110
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014110.3, NP_054829.2
• RefSeq Size: 2336 bp
• RefSeq ORF: 1056 bp
• Locus ID: 5511
• UniProt ID: Q12972
• Cytogenetics: 1p35.3
• Domains: FHA
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) is the shortest transcript but encodes the predominant and longest isoform (alpha). Isoform alpha contains protein phosphatase inhibitory actvity.