Arg 3.1 (ARC) (NM_015193) Human Untagged Clone

CAT#: SC114711

ARC (untagged)-Human activity-regulated cytoskeleton-associated protein (ARC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Arg 3.1
• Synonyms: Arg3.1; hArc
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114711 sequence for NM_015193 edited (data generated by NextGen Sequencing)
  ATGGAGCTGGACCACCGGACCAGCGGCGGGCTCCACGCCTACCCCGGGCCGCGGGGCGGG
  CAGGTGGCCAAGCCCAACGTGATCCTGCAGATCGGGAAGTGCCGGGCCGAGATGCTGGAG
  CACGTGCGGCGGACGCACCGGCACCTGCTGGCCGAGGTGTCCAAGCAGGTGGAGCGCGAG
  CTGAAGGGGCTGCACCGGTCGGTCGGGAAGCTGGAGAGCAACCTGGACGGCTACGTGCCC
  ACGAGCGACTCGCAGCGCTGGAAGAAGTCCATCAAGGCCTGCCTGTGCCGCTGCCAGGAG
  ACCATCGCCAACCTGGAGCGCTGGGTCAAGCGCGAGATGCACGTGTGGCGCGAGGTGTTC
  TACCGCCTGGAGCGCTGGGCCGACCGCCTGGAGTCCACGGGCGGCAAGTACCCGGTGGGC
  AGCGAGTCAGCCCGCCACACCGTTTCCGTGGGCGTGGGGGGTCCCGAGAGCTACTGCCAC
  GAGGCAGATGGCTACGACTACACCGTCAGCCCCTACGCCATCACCCCGCCCCCAGCCGCT
  GGCGAGCTGCCCGGGCAGGAGCCCGCCGAGGCCCAGCAGTACCAGCCGTGGGTCCCCGGC
  GAGGACGGGCAGCCCAGCCCCGGCGTGGACACGCAGATCTTCGAGGACCCTCGAGAGTTC
  CTGAGCCACCTAGAGGAGTACTTGCGGCAGGTGGGCGGCTCTGAGGAGTACTGGCTGTCC
  CAGATCCAGAATCACATGAACGGGCCGGCCAAGAAGTGGTGGGAGTTCAAGCAGGGCTCC
  GTGAAGAACTGGGTGGAGTTCAAGAAGGAGTTCCTGCAGTACAGCGAGGGCACGCTGTCC
  CGAGAGGCCATCCAGCGCGAGCTGGACCTGCCGCAGAAGCAGGGCGAGCCGCTGGACCAG
  TTCCTGTGGCGCAAGCGGGACCTGTACCAGACGCTCTACGTGGACGCGGACGAGGAGGAG
  ATCATCCAGTACGTGGTGGGCACCCTGCAGCCCAAGCTCAAGCGTTTCCTGCGCCACCCC
  CTGCCCAAGACCCTGGAGCAGCTCATCCAGAGGGGCATGGAGGTGCAGGATGACCTGGAG
  CAGGCGGCCGAGCCGGCCGGCCCCCACCTCCCGGTGGAGGATGAGGCGGAGACCCTCACG
  CCCGCCCCCAACAGCGAGTCCGTGGCCAGTGACCGGACCCAGCCCGAGTAG
  
  Clone variation with respect to NM_015193.3
  489 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015193 unedited
  TATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCGAATTCGGCACG
  AGGTCCCTCCGCAGCAGCCGAGCCGGACCTGCCTCCCCGGGCGTGCTCCGCCGGCCCCGC
  CGCCGGCCCGCAGCGACAGACAGGCGCTCCCCGCAGCTCCGCACGGGACCCAGGCCGCCG
  GACCCCAGCGCCGGACCACCGTCCGTCCGCCCCGAGGAGTTTGCCGCCTGCCGGAGCACC
  TGCGCACAGATGGAGCTGGACCACCGGACCAGCGGCGGGCTCCACGCCTACCCCGGGCCG
  CGGGGCGGGCAGGTGGCCAAGCCCAACGTGATCCTGCAGATCGGGAAGTGCCGGGCCGAG
  ATGCTGGAGCACGTGCGGCGGACGCACCGGCACCTGCTGGCCGAGGTGTCCAAGCAGGTG
  GAGCGCGAGCTGAAGGGGCTGCACCGGTCGGTCGGGAAGCTGGAGAGCAACCTGGACGGC
  TACGTGCCCACGAGCGACTCGCAGCGCTGGAAGAAGTCCATCAAGGCCTGCCTGTGCCGC
  TGCCAGGAGACCATCGCCAACCTGGAGCGCTGGGTCAAGCGCGAGATGCACGTGTGGCGC
  GAGGTGTTCTACCGCCTGGAGCGCTGGGCCGACCGCCTGGAGTCCACGGGCGGCAAGTAC
  CCGGTGGGCAGCGAGTCAGCCCGNCACACCGTNTNCGTGGGCGTGGGGGGGTCCCGAGAG
  CTACTGCCACGAGGCAGATGGCTACGACTACACCGTNAGCCCCTACGCCATCACCCCGNC
  CCCAGCCGCTGGCGAGCTGCCCGGGCAGGAGCCCGNCGAGGCCAGCAGTACCAGCCGTGG
  GGTCCCGGCGAGGGACGGCAGNCCAGNCCCCGGGCGTGGACACGCAGATCTTNCNAGGAC
  CCTNCGAGAGNTNNCTGAGCCACCTANAGGAGTACTTGCGNGCAGGTGGGCGGCTTTGTA
  GGAGTACTGCTGNTCCANATCCAAATACATGACGGGCGCCANAAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015193 unedited
  TTTGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAGTTTCAGTGT
  TTTATTAACAAAATCTTACAAAAAGAGTCTGTCTCTGGGGTAAGGTGCACAGGGCCTGGA
  CCGACATTTAAGGCCTGGTCTGTGTGGGGCAAGGGGCAGGGGTTGGCCACAGCCTCATGA
  GGTTTCCAGGGTCTTCACAGGCTCCAGGGCTTCCCCAAAGCCTGTGCCAGCCTTGAGGAT
  TGGTTATGGTTTATGTGCAAAAATAAAATCTGTGGGGCTGGCAGGGTGTGGGCTGAAGAC
  TTGTCAGAGTGGTCTGCAGGGCTGGTCCCCCAGTGGACAAGGCAGAACGATGAACCCTGA
  GCCAGCACCTGCTTAGGCTGCAGACGCTAGTGGCCCACCAGCACCAGGGTTCTGGTGTCG
  GGGCAGGGCAGAATGCCTGGAAGCGCCCCAGGAGCCATGGCTTCTGGGATGTCCTTCCTG
  CAGCTGAGCTCAGGGCTGCTGGGCAGGGGTAGGAGCTGTGGCCCCAGCCTTGGGGCTCAT
  GAAAGCCTCTGGCACAGAGGATGGCCTGGCANCGGGCGGGGTTTCCCTGCTGGGAANGAN
  GCAGGGCCCGACTCCTGCTGCAGTGGCTCTGGGGGACGGCCAGTTGGCGGCCAGCGGGGC
  CGGGCTTAGTGGCNGGGCCCTGGTCCTCGCGCCTGCGCTCAGGCTAANCCGGGGGTTCCT
  GCCCTCTGGTGGACCCGACTCCCCTCTGTACCCCCCTTTGGGGTGGCCTTGGGACTTCCC
  CTTCCACCCAGGAACCGCAGGGGGACGGGGGGGTTGGGCCCCAACACAATCTACACAAAC
  TCTCGGCGACCCCACGAACCCAGGTAAGCTTCCTGAACCCTGCGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015193
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015193.3, NP_056008.1
• RefSeq Size: 2985 bp
• RefSeq ORF: 1191 bp
• Locus ID: 23237
• UniProt ID: Q7LC44
• Cytogenetics: 8q24.3
• Gene Summary: Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (By similarity). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (By similarity). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (By similarity).[UniProtKB/Swiss-Prot Function]