GGA2 (NM_015044) Human Untagged Clone

SKU SC114703

GGA2 (untagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 2 (GGA2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GGA2
• Synonyms: VEAR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114703 sequence for NM_015044 edited (data generated by NextGen Sequencing); ATGGCGGCGACCGCGGTGGCGGCGGCTGTGGCGGGAACCGAGTCGGCCCAGGGTCCCCCG; GGCCCGGCAGCGTCGCTGGAGCTGTGGCTCAACAAAGCCACAGACCCAAGCATGTCGGAA; CAGGATTGGTCAGCTATCCAGAATTTCTGTGAGCAGGTGAACACTGACCCCAATGGCCCC; ACACATGCGCCCTGGCTACTGGCCCACAAGATCCAGTCTCCGCAAGAGAAGGAAGCTCTT; TATGCCTTAACGGTGCTGGAGATGTGCATGAACCACTGTGGGGAGAAGTTCCACAGCGAG; GTGGCCAAATTTCGTTTCCTGAACGAACTGATCAAAGTGTTGTCCCCAAAGTACCTGGGG; TCCTGGGCCACAGGAAAAGTTAAAGGAAGAGTCATTGAAATACTCTTCAGTTGGACAGTC; TGGTTTCCGGAAGACATCAAGATTCGAGACGCTTATCAGATGCTGAAGAAACAAGGAATT; ATAAAACAAGACCCTAAACTACCAGTGGATAAAATCTTACCCCCACCATCTCCCTGGCCC; AAGAGCTCCATCTTTGATGCTGATGAAGAAAAGTCCAAGCTTCTGACAAGGCTTCTAAAG; AGCAACCACCCCGAGGACCTTCAGGCTGCAAACCGGTTAATCAAGAATTTGGTCAAGGAG; GAACAAGAAAAATCGGAGAAGGTGTCCAAGAGGGTCAGTGCGGTGGAGGAAGTGCGAAGC; CATGTGAAGGTGCTGCAGGAGATGCTGAGCATGTACCGCAGGCCAGGGCAGGCCCCGCCC; GACCAGGAGGCCCTGCAGGTCGTGTATGAGAGGTGTGAAAAGCTGCGGCCCACGCTGTTC; CGGTTGGCGAGTGACACCACTGATGACGATGATGCACTCGCGGAAATTCTCCAGGCAAAT; GACCTCCTCACCCAAGGAGTTCTGCTGTACAAACAGGTGATGGAGGGCCGGGTCACCTTT; GGAAACAGAGTGACCAGCTCATTGGGAGACATCCCTGTCTCCAGAGTCTTTCAGAATCCA; GCAGGCTGCATGAAGACCTGCCCCCTGATTGACTTGGAGGTGGACAATGGACCTGCGCAG; ATGGGGACTGTGGTGCCATCTTTGCTTCATCAGGACCTGGCAGCCTTGGGAATCAGTGAT; GCTCCTGTTACAGGCATGGTTTCTGGTCAGAATTGCTGTGAGGAAAAGAGGAATCCCTCC; TCCAGCACGCTGCCAGGCGGTGGTGTTCAGAACCCTTCTGCAGACAGGAATTTGCTGGAC; CTCCTCTCAGCACAGCCAGCTCCGTGCCCTCTGAATTATGTTTCGCAGAAAAGTGTCCCC; AAGGAAGTGCCACCAGGTACTAAGTCCTCTCCAGGTTGGTCCTGGGAGGCTGGCCCGTTG; GCTCCTTCCCCATCTTCACAGAATACACCTCTGGCTCAAGTGTTTGTCCCTTTGGAGTCT; GTTAAGCCCAGCAGCCTGCCGCCTCTCATTGTGTATGACCGGAATGGATTCAGAATTCTG; CTCCACTTCTCCCAGACGGGAGCCCCTGGGCACCCAGAGGTACAGGTGCTGCTCTTGACC; ATGATGAGCACGGCTCCCCAGCCTGTCTGGGATATCATGTTTCAAGTGGCTGTGCCAAAG; TCAATGAGAGTGAAGCTGCAGCCGGCATCCAGCTCCAAGCTTCCTGCATTCAGTCCTTTG; ATGCCTCCAGCTGTGATATCTCAGATGCTGCTGCTTGACAATCCACACAAAGAACCTATC; CGCTTACGGTACAAGCTGACATTCAACCAAGGTGGACAGCCTTTCAGCGAAGTAGGAGAA; GTGAAAGACTTCCCAGACCTGGCTGTCTTGGGCGCAGCCTAA; ; Clone variation with respect to NM_015044.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015044 unedited; TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGAGGAGAGCGCCAGG; ACGCTACAGCGGCTGAAGAGGCAGTGGCGCCCGCGGCCGCAGCGTCGGGGCTGGAGCGAT; GGCGGCGACCGCGGTGGCGGCGGCTGTGGCGGGAACCGAGTCGGCCCAGGGTCCCCCGGG; CCCGGCAGCGTCGCTGGAGCTGTGGCTCAACAAAGCCACAGACCCAAGCATGTCGGAACA; GGATTGGTCAGCTATCCAGAATTTCTGTGAGCAGGTGAACACTGACCCCAATGGCCCCAC; ACATGCGCCCTGGCTACTGGCCCACAAGATCCAGTCTCCGCAAGAGAAGGAAGCTCTTTA; TGCCTTAACGGTGCTGGAGATGTGCATGAACCACTGTGGGGAGAAGTTCCACAGCGAGGT; GGCCAAATTTCGTTTCCTGAACGAACTGATCAAAGTGTTGTCCCCAAAGTACCTGGGGTC; CTGGGCCACAGGAAAAGTTAAAGGAAGAGTCATTGAAATACTCTTCAGTTGGACAGTCTG; GTTTCCGGAAGACATCAAGATTCGAGACGCTTATCAGATGCTGAAGAAACAAGGAATTAT; AAAACAAGACCCTAAACTACCAGTGGATAAAATCTTACCCCCACCATCTCCCTGGCCCAA; GAGCTCCATCTTTGATGCTGATGAAGAAAAGTCCAAGCTTCTGANCAGGCTTCTAAAGAG; CAACCCNCCCCGAGGACCTTCAGCTGCANACCGGGTTATCAAGAANTTNTGGTCAGGGAG; GACAAGAANATCGCGANAAGGTGTCCCAGAAGGTCANTGCGCGTGGAAAGAAGTGCGAAC; TTTTTTAAAGGTGCTGCAAGAAATGCTGAGCTTTTNACCGCAGCCCAAGGGCAGGCCCCG; CGAACAGAGCGCCCTCCNAGGTCTGTTTTTAAAAGAAGGGTGATAGCTGCGCCACCCTTT; TTCCGCGGTTGGAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015044 unedited; ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTAAAAATATCAGATAT; TGTAATTAAGAGCTCCAAACAAGTCTTGCAGTTTCAAAGGTAGAAACCCACCCAGCCAGT; TCAGCTGCTTGGACTTCAAAGCCCTCCGCCTAGCCATCTCAGCCAGGCTCAGGTTCCTTC; TCCCACCCATCAGGCCAAGCAGGACTTGTCAAACATACACATTCAAGTTCCTAGCACACA; GTAGGTGCTAAGTGGGAATTGATTATAAACTTGAATTCTTCCATCAACAAATATCTACCT; CTCCTGTCCAGCTTGCCTCAGATCTTCAGGTTCTCTCTTCTCTGAGGCAGCTAAGCTTCT; ACATCCTTCATGAAGTTTCCTTTACTTCTCGACAGAAGACAGTTCCCTTTAGGCCTATCA; TCTCTAAATGCAACTGGCTTTCCATTTTCCACTTACTCAACACTCCACAAAGTACACTTG; CTACATGGTATTTATTCAATGACTGTATATTTAGTCACCACCTCAATCACTGCTAAGCTC; TCTTCATGTCTATCTGTACCAGTTTCCCATCTTANACTGTGACGTCAGCTGGCAAGGGCT; GGCTGTTGGCCGATTCATCATGACTGACATGGTGTGTCACAAAGAGCTCCAAGTAAATGC; TGTGAAGGAAGAGGATGAGGATGGAGATGAGGATCCTGAGAGGGCCCAAACTGGCTGCCC; AACCCATGGACCTGAAGCCCGAGTCAAGCCTCGGAGCTGTTTTCCCTCTGCCAAGGAACT; NTTGTCAGGGGCCGATTTAAAGCCCATGGGGAGAGGACCTGATGGCCCCCCCATGGGGGC; CCTCAAGCCTCATGCGACCCCTCGCCTGGGAACGGGGAACAAGTTTCCCTTTAAAGAATT; TCCCACAACCCCAGAAACTTGCCCTAGGAAAAGGGACCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_015044
• Insert Size: 3410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015044.2, NP_055859.1
• RefSeq Size: 5163 bp
• RefSeq ORF: 1842 bp
• Locus ID: 23062
• UniProt ID: Q9UJY4
• Cytogenetics: 16p12.2
• Domains: Alpha_adaptinC2, GAT, VHS
• Protein Pathways: Lysosome
• Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1), as compared to transcript variant 2.