GGA2 (NM_015044) Human Untagged Clone

CAT#: SC114703

GGA2 (untagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 2 (GGA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GGA2
• Synonyms: VEAR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114703 sequence for NM_015044 edited (data generated by NextGen Sequencing)
  ATGGCGGCGACCGCGGTGGCGGCGGCTGTGGCGGGAACCGAGTCGGCCCAGGGTCCCCCG
  GGCCCGGCAGCGTCGCTGGAGCTGTGGCTCAACAAAGCCACAGACCCAAGCATGTCGGAA
  CAGGATTGGTCAGCTATCCAGAATTTCTGTGAGCAGGTGAACACTGACCCCAATGGCCCC
  ACACATGCGCCCTGGCTACTGGCCCACAAGATCCAGTCTCCGCAAGAGAAGGAAGCTCTT
  TATGCCTTAACGGTGCTGGAGATGTGCATGAACCACTGTGGGGAGAAGTTCCACAGCGAG
  GTGGCCAAATTTCGTTTCCTGAACGAACTGATCAAAGTGTTGTCCCCAAAGTACCTGGGG
  TCCTGGGCCACAGGAAAAGTTAAAGGAAGAGTCATTGAAATACTCTTCAGTTGGACAGTC
  TGGTTTCCGGAAGACATCAAGATTCGAGACGCTTATCAGATGCTGAAGAAACAAGGAATT
  ATAAAACAAGACCCTAAACTACCAGTGGATAAAATCTTACCCCCACCATCTCCCTGGCCC
  AAGAGCTCCATCTTTGATGCTGATGAAGAAAAGTCCAAGCTTCTGACAAGGCTTCTAAAG
  AGCAACCACCCCGAGGACCTTCAGGCTGCAAACCGGTTAATCAAGAATTTGGTCAAGGAG
  GAACAAGAAAAATCGGAGAAGGTGTCCAAGAGGGTCAGTGCGGTGGAGGAAGTGCGAAGC
  CATGTGAAGGTGCTGCAGGAGATGCTGAGCATGTACCGCAGGCCAGGGCAGGCCCCGCCC
  GACCAGGAGGCCCTGCAGGTCGTGTATGAGAGGTGTGAAAAGCTGCGGCCCACGCTGTTC
  CGGTTGGCGAGTGACACCACTGATGACGATGATGCACTCGCGGAAATTCTCCAGGCAAAT
  GACCTCCTCACCCAAGGAGTTCTGCTGTACAAACAGGTGATGGAGGGCCGGGTCACCTTT
  GGAAACAGAGTGACCAGCTCATTGGGAGACATCCCTGTCTCCAGAGTCTTTCAGAATCCA
  GCAGGCTGCATGAAGACCTGCCCCCTGATTGACTTGGAGGTGGACAATGGACCTGCGCAG
  ATGGGGACTGTGGTGCCATCTTTGCTTCATCAGGACCTGGCAGCCTTGGGAATCAGTGAT
  GCTCCTGTTACAGGCATGGTTTCTGGTCAGAATTGCTGTGAGGAAAAGAGGAATCCCTCC
  TCCAGCACGCTGCCAGGCGGTGGTGTTCAGAACCCTTCTGCAGACAGGAATTTGCTGGAC
  CTCCTCTCAGCACAGCCAGCTCCGTGCCCTCTGAATTATGTTTCGCAGAAAAGTGTCCCC
  AAGGAAGTGCCACCAGGTACTAAGTCCTCTCCAGGTTGGTCCTGGGAGGCTGGCCCGTTG
  GCTCCTTCCCCATCTTCACAGAATACACCTCTGGCTCAAGTGTTTGTCCCTTTGGAGTCT
  GTTAAGCCCAGCAGCCTGCCGCCTCTCATTGTGTATGACCGGAATGGATTCAGAATTCTG
  CTCCACTTCTCCCAGACGGGAGCCCCTGGGCACCCAGAGGTACAGGTGCTGCTCTTGACC
  ATGATGAGCACGGCTCCCCAGCCTGTCTGGGATATCATGTTTCAAGTGGCTGTGCCAAAG
  TCAATGAGAGTGAAGCTGCAGCCGGCATCCAGCTCCAAGCTTCCTGCATTCAGTCCTTTG
  ATGCCTCCAGCTGTGATATCTCAGATGCTGCTGCTTGACAATCCACACAAAGAACCTATC
  CGCTTACGGTACAAGCTGACATTCAACCAAGGTGGACAGCCTTTCAGCGAAGTAGGAGAA
  GTGAAAGACTTCCCAGACCTGGCTGTCTTGGGCGCAGCCTAA
  
  Clone variation with respect to NM_015044.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015044 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGAGGAGAGCGCCAGG
  ACGCTACAGCGGCTGAAGAGGCAGTGGCGCCCGCGGCCGCAGCGTCGGGGCTGGAGCGAT
  GGCGGCGACCGCGGTGGCGGCGGCTGTGGCGGGAACCGAGTCGGCCCAGGGTCCCCCGGG
  CCCGGCAGCGTCGCTGGAGCTGTGGCTCAACAAAGCCACAGACCCAAGCATGTCGGAACA
  GGATTGGTCAGCTATCCAGAATTTCTGTGAGCAGGTGAACACTGACCCCAATGGCCCCAC
  ACATGCGCCCTGGCTACTGGCCCACAAGATCCAGTCTCCGCAAGAGAAGGAAGCTCTTTA
  TGCCTTAACGGTGCTGGAGATGTGCATGAACCACTGTGGGGAGAAGTTCCACAGCGAGGT
  GGCCAAATTTCGTTTCCTGAACGAACTGATCAAAGTGTTGTCCCCAAAGTACCTGGGGTC
  CTGGGCCACAGGAAAAGTTAAAGGAAGAGTCATTGAAATACTCTTCAGTTGGACAGTCTG
  GTTTCCGGAAGACATCAAGATTCGAGACGCTTATCAGATGCTGAAGAAACAAGGAATTAT
  AAAACAAGACCCTAAACTACCAGTGGATAAAATCTTACCCCCACCATCTCCCTGGCCCAA
  GAGCTCCATCTTTGATGCTGATGAAGAAAAGTCCAAGCTTCTGANCAGGCTTCTAAAGAG
  CAACCCNCCCCGAGGACCTTCAGCTGCANACCGGGTTATCAAGAANTTNTGGTCAGGGAG
  GACAAGAANATCGCGANAAGGTGTCCCAGAAGGTCANTGCGCGTGGAAAGAAGTGCGAAC
  TTTTTTAAAGGTGCTGCAAGAAATGCTGAGCTTTTNACCGCAGCCCAAGGGCAGGCCCCG
  CGAACAGAGCGCCCTCCNAGGTCTGTTTTTAAAAGAAGGGTGATAGCTGCGCCACCCTTT
  TTCCGCGGTTGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015044 unedited
  ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTAAAAATATCAGATAT
  TGTAATTAAGAGCTCCAAACAAGTCTTGCAGTTTCAAAGGTAGAAACCCACCCAGCCAGT
  TCAGCTGCTTGGACTTCAAAGCCCTCCGCCTAGCCATCTCAGCCAGGCTCAGGTTCCTTC
  TCCCACCCATCAGGCCAAGCAGGACTTGTCAAACATACACATTCAAGTTCCTAGCACACA
  GTAGGTGCTAAGTGGGAATTGATTATAAACTTGAATTCTTCCATCAACAAATATCTACCT
  CTCCTGTCCAGCTTGCCTCAGATCTTCAGGTTCTCTCTTCTCTGAGGCAGCTAAGCTTCT
  ACATCCTTCATGAAGTTTCCTTTACTTCTCGACAGAAGACAGTTCCCTTTAGGCCTATCA
  TCTCTAAATGCAACTGGCTTTCCATTTTCCACTTACTCAACACTCCACAAAGTACACTTG
  CTACATGGTATTTATTCAATGACTGTATATTTAGTCACCACCTCAATCACTGCTAAGCTC
  TCTTCATGTCTATCTGTACCAGTTTCCCATCTTANACTGTGACGTCAGCTGGCAAGGGCT
  GGCTGTTGGCCGATTCATCATGACTGACATGGTGTGTCACAAAGAGCTCCAAGTAAATGC
  TGTGAAGGAAGAGGATGAGGATGGAGATGAGGATCCTGAGAGGGCCCAAACTGGCTGCCC
  AACCCATGGACCTGAAGCCCGAGTCAAGCCTCGGAGCTGTTTTCCCTCTGCCAAGGAACT
  NTTGTCAGGGGCCGATTTAAAGCCCATGGGGAGAGGACCTGATGGCCCCCCCATGGGGGC
  CCTCAAGCCTCATGCGACCCCTCGCCTGGGAACGGGGAACAAGTTTCCCTTTAAAGAATT
  TCCCACAACCCCAGAAACTTGCCCTAGGAAAAGGGACCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015044
• Insert Size: 3410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015044.2, NP_055859.1
• RefSeq Size: 5163 bp
• RefSeq ORF: 1842 bp
• Locus ID: 23062
• UniProt ID: Q9UJY4
• Cytogenetics: 16p12.2
• Domains: VHS, GAT, Alpha_adaptinC2
• Protein Pathways: Lysosome
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1), as compared to transcript variant 2.