CHCHD2 (NM_016139) Human Untagged Clone

CAT#: SC114478

CHCHD2 (untagged)-Human coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHCHD2
• Synonyms: C7orf17; MIX17B; MNRR1; NS2TP; PARK22
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114478 sequence for NM_016139 edited (data generated by NextGen Sequencing)
  ATGCCGCGTGGAAGCCGAAGCCGCACCTCCCGCATGGCCCCTCCGGCCAGCCGGGCCCCT
  CAGATGAGAGCTGCACCCAGGCCAGCACCAGTCGCTCAGCCACCAGCAGCGGCACCCCCA
  TCTGCAGTTGGCTCTTCTGCTGCTGCGCCCCGGCAGCCAGGTCTGATGGCCCAGATGGCA
  ACCACTGCAGCTGGCGTGGCTGTGGGCTCTGCTGTGGGGCACACATTGGGTCACGCCATT
  ACTGGGGGCTTCAGTGGAGGAAGTAATGCTGAGCCTGCGAGGCCTGACATCACTTACCAG
  GAGCCTCAGGGAACCCAGCCAGCACAGCAGCAGCAGCCTTGCCTCTATGAGATCAAACAG
  TTTCTGGAGTGTGCCCAGAACCAGGGTGACATCAAGCTCTGTGAGGGTTTCAATGAGGTG
  CTGAAACAGTGCCGACTTGCAAACGGATTGGCCTAA
  
  Clone variation with respect to NM_016139.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016139 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTCGGTGGTTGTCCC
  ACGTCCGGAGGCCTAGCCGTCGCTTACCTAGGATGCCGCGTGGAAGCCGAAGCCGCACCT
  CCCGCATGGCCCCTCCGGCCAGCCGGGCCCCTCAGATGAGAGCTGCACCCAGGCCAGCAC
  CAGTCGCTCAGCCACCAGCAGCGGCACCCCCATCTGCAGTTGGCTCTTCTGCTGCTGCGC
  CCCGGCAGCCAGGTCTGATGGCCCAGATGGCAACCACTGCAGCTGGCGTGGCTGTGGGCT
  CTGCTGTGGGGCACACATTGGGTCACGCCATTACTGGGGGCTTCAGTGGAGGAAGTAATG
  CTGAGCCTGCGAGGCCTGACATCACTTACCAGGAGCCTCAGGGAACCCAGCCAGCACAGC
  AGCAGCAGCCTTGCCTCTATGAGATCAAACAGTTTCTGGAGTGTGCCCAGAACCAGGGTG
  ACATCAAGCTCTGTGAGGGTTTCAATGAGGTGCTGAAACAGTGCCGACTTGCAAACGGAT
  TGGCCTAATGAAGAAGTTCAACCTGGAGAGATGGAAAATCAGCTCTCATAACTAAGTTAA
  TTTAGTATAAAAATAGAATTGATAGTGAGGGTATAAAGTGTAACCATCAGTTAAACCTCT
  CCTGTCATTCCTGGCTTCCTTTGCTCAGAATTGAAATGGAAGTGGGGGGTGCCCNTACTC
  TGTANAATCTGGGACTGGGCANATGGTTGTGTGGCCTCCNTAAACTAGCTGGTATGGTAT
  GATTNTATNCTTTTGGGAGTTAAATAGAAATAAGTCATTNTCTTNCCAGGTATGGGNTTA
  AAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAG
  ATCCCGGGTGGGATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016139 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAACCATACCTTGGAAGAAAAT
  GACTTTATTCTAATTAACTCACAAAGAATAAAATCATAACATAACAGCTAGTTTAAGGAG
  GCCACACAAACATTTGCCCAGTCCCAGATTCTACAGAGTAGGGACACCCCCACTTCCATT
  TCAATTCTGAAGCAAGGAAGCCAGGAATGACAGGAGAGGTTTAACTGATGGTTACACTTT
  ATACCCTCACTATCAATTCTATTTTTATACTAAATTAACTTAGTTATGAGAGCTGATTTT
  CCATCTCTCCAGGTTGAACTTCTTCATTAGGCCAATCCGTTTGCAAGTCGGCACTGTTTC
  AGCACCTCATTGAAACCCTCACAGAGCTTGATGTCACCCTGGTTCTGGGCACACTCCAGA
  AACTGTTTGATCTCATAGAGGCAAGGCTGCTGCTGCTGTGCTGGCTGGGTTCCCTGAGGC
  TCCTGGTAAGTGATGTCAGGCCTCGCAGGCTCAGCATTACTTCCTCCACTGAAGCCCCCA
  GTAATGGCGTGACCCAATGTGTGCCCCACAGCAGAGCCCACAGCCACGCCAGCTGCAGTG
  GTTGCCATCTGGGCCATCAGACCTGGCTGCCGGGGCGCAGCAGCAGAAGAGCCAACTGCA
  GATGGGGGTGCCGCTGCTGGTGGCTGANCGACTGGTGCTGGCCTGGGTGCAGCTCTCATC
  TGAGGGGCCCNNGCTGCCGNAGGGGCATGCNGGAGGTGCGGCTTCGGCTCCCGCGGCATC
  CTAGTAAGCGACGGCTAGGCCTCCGGACGTGGGACACCCACCGAAGCCTCGTGCCGATTC
  CGGGCGCCCTATATGAGTCGTATACAAATTCTGAGGGTCACTAACGAGCTTGCTATATAG
  ACCTCCACGTACAGCCTACCGGCATNGCGTAACGGGCCGGGTATTACAACTTTGGAAGGC
  CGCTGATTGGGGCCAAAAACTCCTGGAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016139
• Insert Size: 840 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016139.2, NP_057223.1
• RefSeq Size: 831 bp
• RefSeq ORF: 456 bp
• Locus ID: 51142
• UniProt ID: Q9Y6H1
• Cytogenetics: 7p11.2
• Domains: DUF657
• Gene Summary: The protein encoded by this gene belongs to a class of eukaryotic CX(9)C proteins characterized by four cysteine residues spaced ten amino acids apart from one another. These residues form disulfide linkages that define a CHCH fold. In response to stress, the protein translocates from the mitochondrial intermembrane space to the nucleus where it binds to a highly conserved 13 nucleotide oxygen responsive element in the promoter of cytochrome oxidase 4I2, a subunit of the terminal enzyme of the electron transport chain. In concert with recombination signal sequence-binding protein J, binding of this protein activates the oxygen responsive element at four percent oxygen. In addition, it has been shown that this protein is a negative regulator of mitochondria-mediated apoptosis. In response to apoptotic stimuli, mitochondrial levels of this protein decrease, allowing BCL2-associated X protein to oligomerize and activate the caspase cascade. Pseudogenes of this gene are found on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus compared to isoform 1.