EGFL7 (NM_016215) Human Untagged Clone

CAT#: SC114429

EGFL7 (untagged)-Human EGF-like-domain, multiple 7 (EGFL7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EGFL7
• Synonyms: NEU1; VE-STATIN; ZNEU1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114429 sequence for NM_016215 edited (data generated by NextGen Sequencing)
  ATGAGGGGCTCTCAGGAGGTGCTGCTGATGTGGCTTCTGGTGTTGGCAGTGGGCGGCACA
  GAGCACGCCTACCGGCCCGGCCGTAGGGTGTGTGCTGTCCGGGCTCACGGGGACCCTGTC
  TCCGAGTCGTTCGTGCAGCGTGTGTACCAGCCCTTCCTCACCACCTGCGACGGGCACCGG
  GCCTGCAGCACCTACCGAACCATCTATAGGACCGCCTACCGCCGCAGCCCTGGGCTGGCC
  CCTGCCAGGCCTCGCTACGCGTGCTGCCCCGGCTGGAAGAGGACCAGCGGGCTTCCTGGG
  GCCTGTGGAGCAGCAATATGCCAGCCGCCATGCCGGAACGGAGGGAGCTGTGTCCAGCCT
  GGCCGCTGCCGCTGCCCTGCAGGATGGCGGGGTGACACTTGCCAGTCAGATGTGGATGAA
  TGCAGTGCTAGGAGGGGCGGCTGTCCCCAGCGCTGCGTCAACACCGCCGGCAGTTACTGG
  TGCCAGTGTTGGGAGGGGCACAGCCTGTCTGCAGACGGTACACTCTGTGTGCCCAAGGGA
  GGGCCCCCCAGGGTGGCCCCCAACCCGACAGGAGTGGACAGTGCAATGAAGGAAGAAGTG
  CAGAGGCTGCAGTCCAGGGTGGACCTGCTGGAGGAGAAGCTGCAGCTGGTGCTGGCCCCA
  CTGCACAGCCTGGCCTCGCAGGCACTGGAGCATGGGCTCCCGGACCCCGGCAGCCTCCTG
  GTGCACTCCTTCCAGCAGCTCGGCCGCATCGACTCCCTGAGCGAGCAGATTTCCTTCCTG
  GAGGAGCAGCTGGGGTCCTGCTCCTGCAAGAAAGACTCGTGA
  
  Clone variation with respect to NM_016215.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016215 unedited
  TTAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCGGATCC
  GGCGGCCACCCAGAGGAGAAGGCCACCCCGCCTGGAGGCACAGGCCATGAGGGGCTCTCA
  GGAGGTGCTGCTGATGTGGCTTCTGGTGTTGGCAGTGGGCGGCACAGAGCACGCCTACCG
  GCCCGGCCGTAGGGTGTGTGCTGTCCGGGCTCACGGGGACCCTGTCTCCGAGTCGTTCGT
  GCAGCGTGTGTACCAGCCCTTCCTCACCACCTGCGACGGGCACCGGGCCTGCAGCACCTA
  CCGAACCATCTATAGGACCGCCTACCGCCGCAGCCCTGGGCTGGCCCCTGCCAGGCCTCG
  CTACGCGTGCTGCCCCGGCTGGAAGAGGACCAGCGGGCTTCCTGGGGCCTGTGGAGCAGC
  AATATGCCAGCCGCCATGCCGGAACGGAGGGAGCTGTGTCCAGCCTGGCCGCTGCCGCTG
  CCCTGCAGGATGGCGGGGTGACACTTGCCAGTCAGATGTGGATGAATGCAGTGCTAGGAG
  GGGCGGCTGTCCCCAGCGCTGCGTCAACACCGCCGGCAGTTACTGGTGCCAGTGTTGGGA
  GGGGCACAGCCTGTCTGCAGACGGTACACTCTGTGTGCCNCAAGGAGGGCCCCCCAGGGT
  GGCCCCCAACCCGACAGGAGTGGACAGTGCAATGAAGGAAGAAGTGCAGAGGCTGCAGTC
  CAGGGTGGACCTGCTGGAGGAGAGCTGCAGCTGGTGCTGGCCCCACTGCACAGCCTGGCC
  TCGCNAGCACTGGAGCATGGGCTCCCNGACCCGGCAGCCTNCTGGTGCACNTCTTNCAGC
  AGCTCGGCGCATCGACTCCCTGAGCGAGCAGATTTCCTTTCTGGAGAGCAGCTGGGGTCC
  TGCTCCTGCANGAAGACTCGTGACTGCCCAGCGCCCAGCTGGNACTGAGCCCTCACGCCG
  CCTGCAGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016215 unedited
  CCTCTCTTTTTTTGTGCTGGGGGTGCAGGNNCAGCAGCCCCCACTGNAGGCCCCACCCAG
  CCTCCGGGCTGCCTGGCCTGTGCCATGGGTCCCAGGCTCCAGCAGGGAGCTCGTACCTTC
  CCTCAGCTGAGGGCCCACCTGGCCTTGGGATGCCGTTGGGGTAGCCAGGGTGGGGGTAGC
  CAGGGGTGGATTCACAGAGAAGATCCCAGCCCATCCCATGCCAGGGTCTGGGGAGCCTCC
  CGAGGAAGGGGAGGAGGAAGAGGAGGAAGGCCCTGCCTGGCCTTCCGCTCAGTCACCCCG
  AGGTGGTTTCTGGACCCCCAGCATGTTGGGCAGGGGCATGGGGGCTGCAGGGCGGCGTGA
  GGGGCTCAGTCCAGCCTGGGGCGCTGGGCAGTCACGAGTCTTTCTTGCAGGAGCAGGACC
  CCAGCTGCTCCTCCAGGAAGGAAATCTGCTCGCTCAGGGAGTCGATGCGGCCGAGCTGCT
  GGAAGGAGTGCACCACGAGGCTGCCGGGGTCCGGGAGCCCATGCTTCAGTGCCTGCGAGG
  CCAGGCTGCGCATTGGGGCCAGCACCAGCTGCTGCTTCTCCTCCAGCAGGTTCACCCTGG
  ACTGCAGCCTCTGCACTTTTTACTTCATTGCACTGTCCACTCCTGTCCGGTTTGGGGCCA
  CCCTGGGGGGGCCCTCCTTTGGGCACACATATTGTACCGCTTGCAGACAGGCTGTGCCCC
  TCCCACTCTGGTACCATAACCTGCCGCGGGGTTGAACCACGCTGGGGACAACCGCCCCTT
  CTAGCACTGGATTCATCTACATCTGACTGTAAGTGGCACCCCGCCATCCTGCAGGGACTG
  GCACGGGTCAGCTTGGACAAGATTCCTTCTCTCCCGAGGGCGCTTGGATTTTGCTGGTCC
  CAGGCCCAAGAAACTCTCGGTCTCTTCATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016215
• Insert Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016215.3, NP_057299.1
• RefSeq Size: 1569 bp
• RefSeq ORF: 822 bp
• Locus ID: 51162
• UniProt ID: Q9UHF1
• Cytogenetics: 9q34.3
• Domains: EGF_CA, EGF, EGF
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a secreted endothelial cell protein that contains two epidermal growth factor-like domains. The encoded protein may play a role in regulating vasculogenesis. This protein may be involved in the growth and proliferation of tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (1) represents the longest transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.