Arginase 1 (ARG1) (NM_000045) Human Untagged Clone

SKU SC114331

ARG1 (untagged)-Human arginase, liver (ARG1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Arginase 1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114331 sequence for NM_000045 edited (data generated by NextGen Sequencing); ATGAGCGCCAAGTCCAGAACCATAGGGATTATTGGAGCTCCTTTCTCAAAGGGACAGCCA; CGAGGAGGGGTGGAAGAAGGCCCTACAGTATTGAGAAAGGCTGGTCTGCTTGAGAAACTT; AAAGAACAAGAGTGTGATGTGAAGGATTATGGGGACCTGCCCTTTGCTGACATCCCTAAT; GACAGTCCCTTTCAAATTGTGAAGAATCCAAGGTCTGTGGGAAAAGCAAGCGAGCAGCTG; GCTGGCAAGGTGGCAGAAGTCAAGAAGAACGGAAGAATCAGCCTGGTGCTGGGCGGAGAC; CACAGTTTGGCAATTGGAAGCATCTCTGGCCATGCCAGGGTCCACCCTGATCTTGGAGTC; ATCTGGGTGGATGCTCACACTGATATCAACACTCCACTGACAACCACAAGTGGAAACTTG; CATGGACAACCTGTATCTTTCCTCCTGAAGGAACTAAAAGGAAAGATTCCCGATGTGCCA; GGATTCTCCTGGGTGACTCCCTGTATATCTGCCAAGGATATTGTGTATATTGGCTTGAGA; GACGTGGACCCTGGGGAACACTACATTTTGAAAACTCTAGGCATTAAATACTTTTCAATG; ACTGAAGTGGACAGACTAGGAATTGGCAAGGTGATGGAAGAAACACTCAGCTATCTACTA; GGAAGAAAGAAAAGGCCAATTCATCTAAGTTTTGATGTTGACGGACTGGACCCATCTTTC; ACACCAGCTACTGGCACACCAGTCGTGGGAGGTCTGACATACAGAGAAGGTCTCTACATC; ACAGAAGAAATCTACAAAACAGGGCTACTCTCAGGATTAGATATAATGGAAGTGAACCCA; TCCCTGGGGAAGACACCAGAAGAAGTAACTCGAACAGTGAACACAGCAGTTGCAATAACC; TTGGCTTGTTTCGGACTTGCTCGGGAGGGTAATCACAAGCCTATTGACTACCTTAACCCA; CCTAAGTAA; ; Clone variation with respect to NM_000045.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000045 unedited; TTCACATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGCTCAAGTG; CAGCAAAGAGAAGTGTCAGAGCATGAGCGCCAAGTCCAGAACCATAGGGATTATTGGAGC; TCCTTTCTCAAAGGGACAGCCACGAGGAGGGGTGGAAGAAGGCCCTACAGTATTGAGAAA; GGCTGGTCTGCTTGAGAAACTTAAAGAACAAGAGTGTGATGTGAAGGATTATGGGGACCT; GCCCTTTGCTGACATCCCTAATGACAGTCCCTTTCAAATTGTGAAGAATCCAAGGTCTGT; GGGAAAAGCAAGCGAGCAGCTGGCTGGCAAGGTGGCAGAAGTCAAGAAGAACGGAAGAAT; CAGCCTGGTGCTGGGCGGAGACCACAGTTTGGCAATTGGAAGCATCTCTGGCCATGCCAG; GGTCCACCCTGATCTTGGAGTCATCTGGGTGGATGCTCACACTGATATCAACACTCCACT; GACAACCACAAGTGGAAACTTGCATGGACAACCTGTATCTTTCCTCCTGAAGGAACTAAA; AGGAAAGATTCCCGATGTGCCAGGATTCTCCTGGGTGACTCCCTGTATATCTGCCAAGGA; TATTGTGTATATTGGCTTGAGAGACGTGGACCCTGNGGAACACTACATTTTGAAAACTCT; AGGCATTAAATACTTTTCAATGACTGAAGTGGACAGACTANGAATTGGCAAGGTGATGGA; AGAAACACTCAGCTATCTACTAGGAAGAAAGAAAGGCCATTCATCTAGTTTTGATGTTGA; CGGACTGGACCCATCTTTCACACCAGCTACTGGCACACCAGTCGTGGGAAGGTCTGACAT; ACAGNAAANGGTCTCTACATCACAGAAGAAATCTACAAACAGGGCTACTCTNCAGATTAG; ATTAATGGAAGTGAACCATNCCTGGGNNAGACACAGAGAAGTACTCGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000045 unedited; NNCCCGGGANNNNNCNNAAANAAAAAAAAAACTGACTTGNACCGCGCCGCATACTANGAT; CGGTTTTTTTTTTTTTTTTTTTCTTGTTATAAAAATTTATTATAAAAAACACATTTTTTG; AATGACATGGACACATAGTACCTTTCCACATGTGGTAGCTTTGTCTCCTGATTCCAAGAG; TCCCACTCTATGTATGGGGGCTTATGTAAGTGTGCTTATTTTTAATGCCAGCAAATTTTT; AATTGGAAATCAATGTACACTTCTCTTTCTAAGGATAAGTCTTTTGCCAAGTTAGAAAAT; ATAAGCTTTTAAATTTCAAAAAAGTTAGAATTTCCACATCTTGAATTTTACACCAAGAGG; GAATTTGTAGAGTTTATACTAATTGGAAAAACATTTTTCTGAAAGAACAAGTCTTTAGAA; GGATAACTCTATGCTTAAGAAAATGATTAGCTTTCTAATTATGCCATTAACTATGAGATT; TATATCGGATGTTTCCACATTTACTTAGGTGGGTTAAGGTAGTCAATAGGCTTGTGATTA; CCCTCCCGAGCAAGTCCCGAAACAAGCCAAGGTTATTGCAACTGCTGTGTTCACTGTTCG; AGTTACTTCTTCTGGTGTCTTCCCCAGGAATGGGTTCACTTCCATTATATCTAATCCTGA; GAGTAGCCCTGTTTTGTAGATTTCTTCTGTGATGAAGAGACCCTCCTCTGTATGTCAGAC; CTCCACGACTGGTGTGCCAGAAGCTGGTGTGAAAGATGGTCCACGCCCGCCACATCAAAA; CTTAATGAATTGCCCTTTTCTTTTTTCCTAAGAGAAGCTGAATGTTTCTTCCATCACCTT; GCCAATTCTAAACTGGCCCCTTAGCAATTGAAAAGATTTAATGCCCTAAGTTTCAAAAAT; GTAGTTTCCCAGGGTCCCCTTTTTTAAGCCAATTAACCAATTTTCTTGCCCAAATTCCGG; GAGCACCCCAGGAAAACCGGGCAATCGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000045
• Insert Size: 1570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000045.2, NP_000036.2
• RefSeq Size: 1447 bp
• RefSeq ORF: 969 bp
• Locus ID: 383
• UniProt ID: P05089
• Cytogenetics: 6q23.2
• Domains: arginase
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Summary: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]; Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.