Glutaredoxin 5 (GLRX5) (NM_016417) Human Untagged Clone

CAT#: SC114293

GLRX5 (untagged)-Human glutaredoxin 5 (GLRX5), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glutaredoxin 5
• Synonyms: C14orf87; FLB4739; GRX5; PR01238; PRO1238; PRSA; SIDBA3; SPAHGC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016417, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCGGGTCCCTCGGCCGAGCTGCGGCGGCTCTGCTCCGCTGGGGGCGCGGCGCGGGCGGCGGTGGCC
  TTTGGGGTCCGGGCGTGCGGGCGGCGGGCTCGGGCGCGGGCGGCGGCGGCTCGGCGGAGCAGTTGGACGC
  GCTGGTGAAGAAGGACAAGGTGGTGGTCTTCCTCAAGGGGACGCCGGAGCAGCCCCAGTGCGGCTTCAGC
  AACGCCGTGGTGCAGATCCTGCGGCTGCACGGCGTCCGCGATTACGCGGCCTACAACGTGCTGGACGACC
  CGGAGCTCCGACAAGGCATTAAAGACTATTCCAACTGGCCCACCATCCCGCAAGTGTACCTCAATGGCGA
  GTTTGTAGGGGGCTGTGACATTCTTCTGCAGATGCACCAGAATGGGGACTTGGTGGAAGAACTGAAAAAG
  CTGGGGATCCACTCCGCCCTTTTAGATGAAAAGAAAGACCAAGACTCCAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016417 unedited
  TGTAATACGACTCATCTATAGGGCGGCCGCGAATTCGCACGAGGGCCGTCGTGGGCTCCG
  GCTTGCGTGCGGAGATGAGCGGGTCCCTCGGCCGAGCTGCGGCGGCTCTGCTCCGCTGGG
  GGCGCGGCGCGGGCGGCGGTGGCCTTTGGGGTCCGGGCGTGCGGGCGGCGGGCTCGGGCG
  CGGGCGGCGGCGGCTCGGCGGAGCAGTTGGACGCGCTGGTGAAGAAGGACAAGGTGGTGG
  TCTTCCTCAAGGGGACGCCGGAGCAGCCCCAGTGCGGCTTCAGCAACGCCGTGGTGCAGA
  TCCTGCGGCTGCACGGCGTCCGCGATTACGCGGCCTACAACGTGCTGGACGACCCGGAGC
  TCCGACAAGGCATTAAAGACTATTCCAACTGGCCCACCATCCCGCAAGTGTACCTCAATG
  GCGAGTTTGTAGGGGGCTGTGACATTCTTCTGCAGATGCACCAGAATGGGGACTTGGTGG
  AAGAACTGAAAAAGCTGGGGATCCACTCCGCCCTTTTAGATGAAAAGAAAGACCAAGACT
  CCAAGTGAGGGCGGCCAAGTCCTCGCTGAGCAGAGAGGGAGCCGTTCATGTCAGAGACTC
  ACTGCCAGAAAAGCCTTACCCATTTTGGTTTTCACTATTGAGACCGCAACTGCCTGCACT
  GATCATTTTGGTTCGTGACCAGNTGGTGATTTTAGTTGGTCTGGTGTTCGGGCTACCATA
  TTNTATTGTGGACCTATTACAACCACTGCACTGTAAAGATTCATTGCTGGATAATGATAT
  TGGTGTAAACAAAATTCATTTTAATTTGGCACCTAATCCTTGCCCTGATCAAAAAGTTAA
  AAGGGACCTTTGGAATCATAATTCATGAACCCCTTTGGCAATGGGCATTTTCCAAAAAAA
  GTTCTTCCCCCAAAAANAAAAAAAAAATTCACTTTAAATTGCCGCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016417 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGGGGGAGATACTCTCT
  TTGGAGACTGACACATTTGCAGAGGGGTCATGAATAATGATTCCAAAGCTCCTATTTAAC
  TTCTGAATCAGGCAAAGAATAAGTGACAATATAAGAATGAATTTTGTTTACAGCAATATC
  ATAATACAGCATTGAATCATTACAGTGCAGTGGTTGTAATTAAGTCCACAATAAAATATT
  CTTAGCCCGAACACCAGACCAACTAAAATCACCAACTGCTCACGAACCAAAATGATCAGT
  GCAAGCAGTTGCGGTCTCAATAGTGAAAACCAAAATGGGTAAGGCTTTTCTGGCAGTGAG
  TCTCTGACATGAACGGCTCCCTCTCTGCTCAGCGAGGACTTGGCCGCCCTCACTTGGAGT
  CTTGGTCTTTCTTTTCATCTAAAAGGGCGGAGTGGATCCCCAGCTTTTTCAGTTCTTCCA
  CCAAGTCCCCATTCTGGTGCATCTGCAGAAGAATGTCACAGCCCCCTACAAACTCGCCAT
  TGAGGTACACTTGCGGGATGGTGGGCCACTTGGAATAGCCTTTAATGCCTTGTCGGAGCT
  CCGGGGCCGCCCACCACCTTGGATGGCCCCCTAAACCCGGGACCCCGTGCACCCGCAGGA
  TCTGCACCCCGGGGTTTCTGGAAGCCGGACTGGGGGTTGCCTCCGGGGCCCCCTTGGAGG
  AAAACACCACCCTTGGTCTTTTTCCCCCCCCCTTCCCATGGGTCCCCCCGATCCGCCGCG
  CCTGTGGCCAAAACCCCCGTCCCACCTCCGGACCCCACAGCCCCGGCCGCCCTGCCTGTT
  CCCCCACGGCAAAAACCCCCCACATCCCCCAAGGAACCCTCTCCTCCATCCAACCCGGAT
  CCGCACGGCCTTTTTCCCAATTTTCCCCTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016417
• Insert Size: 930 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016417.2, NP_057501.2
• RefSeq Size: 1182 bp
• RefSeq ORF: 474 bp
• Locus ID: 51218
• UniProt ID: Q86SX6
• Cytogenetics: 14q32.13
• Gene Summary: This gene encodes a mitochondrial protein, which is evolutionarily conserved. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Mutations in this gene are associated with autosomal recessive pyridoxine-refractory sideroblastic anemia. [provided by RefSeq, May 2010]