RNF181 (NM_016494) Human Untagged Clone

CAT#: SC114249

RNF181 (untagged)-Human ring finger protein 181 (RNF181)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF181
• Synonyms: HSPC238
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016494 edited
  ATGGCGTCCTATTTCGATGAACACGACTGCGAGCCGTCGGACCCTGAGCAGGAGACGCGA
  ACCAACATGCTGCTGGAGCTCGCAAGGTCACTTTTCAATAGGATGGACTTTGAAGACTTG
  GGGTTGGTAGTAGATTGGGACCACCACCTGCCTCCACCAGCTGCCAAGACTGTGGTTGAG
  AACCTCCCCAGGACAGTCATCAGAGGCTCTCAGGCTGAGCTCAAGTGCCCCGTGTGTCTT
  TTGGAATTTGAGGAGGAGGAGACTGCCATTGAGATGCCTTGCCATCACCTTTTCCATTCC
  AGCTGCATTCTGCCCTGGCTAAGCAAGACAAATTCCTGTCCCTTGTGCCGCTATGAGCTG
  CCCACTGATGACGACACTTATGAGGAGCACAGACGAGATAAGGCTCGAAAACAGCAGCAG
  CAACACCGACTGGAGAACCTCCATGGAGCCATGTACACGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016494 unedited
  CACGAGGGGCTGTGTCAGAAGGCTGGGCAGCCATGGCGTCCTATTTCGATGAACACGACT
  GCGAGCCGTCGGACCCTGAGCAGGAGACGCGAACCAACATGCTGCTGGAGCTCGCAAGGT
  CACTTTTCAATAGGATGGACTTTGAAGACTTGGGGTTGGTAGTAGATTGGGACCACCACC
  TGCCTCCACCAGCTGCCAAGACTGTGGTTGAGAACCTCCCCAGGACAGTCATCAGAGGCT
  CTCAGGCTGAGCTCAAGTGCCCCGTGTGTCTTTTGGAATTTGAGGAGGAGGAGACTGCCA
  TTGAGATGCCTTGCCATCACCTTTTCCATTCCAGCTGCATTCTGCCCTGGCTAAGCAAGA
  CAAATTCCTGTCCCTTGTGCCGCTATGAGCTGCCCACTGATGACGACACTTATGAGGAGC
  ACAGACGAGATAAGGCTCGAAAACAGCAGCAGCAACACCGACTGGAGAACCTCCATGGAG
  CCATGTACACGTGAGGAGGTTGGGGCTGAGTGCTGGCCCTCTGCGTCTTCCTTATTAACC
  TTGAATCCTCATTAAAGGTTTCTTTACCCACAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  TCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTG
  ACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG
  TCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATA
  
• Restriction Sites: Please inquire
• ACCN: NM_016494
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016494.2, NP_057578.1
• RefSeq Size: 601 bp
• RefSeq ORF: 462 bp
• Locus ID: 51255
• UniProt ID: Q9P0P0
• Cytogenetics: 2p11.2
• Protein Families: Druggable Genome
• Gene Summary: RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).[supplied by OMIM, Dec 2008]