EFEMP2 (NM_016938) Human Untagged Clone

SKU SC114145

EFEMP2 (untagged)-Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: EFEMP2
• Synonyms: ARCL1B; FBLN4; MBP1; UPH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114145 sequence for NM_016938 edited (data generated by NextGen Sequencing); ATGCTCCCCTGCGCCTCCTGCCTACCCGGGTCTCTACTGCTCTGGGCGCTGCTACTGTTG; CTCTTGGGATCAGCTTCTCCTCAGGATTCTGAAGAGCCCGACAGCTACACGGAATGCACA; GATGGCTATGAGTGGGACCCAGACAGCCAGCACTGCCGGGATGTCAACGAGTGTCTGACC; ATCCCTGAGGCCTGCAAGGGGGAAATGAAGTGCATCAACCACTACGGGGGCTACTTGTGC; CTGCCCCGCTCCGCTGCCGTCATCAACGACCTACACGGCGAGGGACCCCCGCCACCAGTG; CCTCCCGCTCAACACCCCAACCCCTGCCCACCAGGCTATGAGCCCGACGATCAGGACAGC; TGTGTGGATGTGGACGAGTGTGCCCAGGCCCTGCACGACTGTCGCCCCAGCCAGGACTGC; CATAACTTGCCTGGCTCCTATCAGTGCACCTGCCCTGATGGTTACCGCAAGATCGGGCCC; GAGTGTGTGGACATAGACGAGTGCCGCTACCGCTACTGCCAGCACCGCTGCGTGAACCTG; CCTGGCTCCTTCCGCTGCCAGTGCGAGCCGGGCTTCCAGCTGGGGCCTAACAACCGCTCC; TGTGTTGATGTGAACGAGTGTGACATGGGGGCCCCATGCGAGCAGCGCTGCTTCAACTCC; TATGGGACCTTCCTGTGTCGCTGCCACCAGGGCTATGAGCTGCATCGGGATGGCTTCTCC; TGCAGTGATATTGATGAGTGTAGCTACTCCAGCTACCTCTGTCAGTACCGCTGCGTCAAC; GAGCCAGGCCGTTTCTCCTGCCACTGCCCACAGGGTTACCAGCTGCTGGCCACACGCCTC; TGCCAAGACATTGATGAGTGTGAGTCTGGTGCGCACCAGTGCTCCGAGGCCCAAACCTGT; GTCAACTTCCATGGGGGCTACCGCTGCGTGGACACCAACCGCTGCGTGGAGCCCTACATC; CAGGTCTCTGAGAACCGCTGTCTCTGCCCGGCCTCCAACCCTCTATGTCGAGAGCAGCCT; TCATCCATTGTGCACCGCTACATGACCATCACCTCGGAGCGGAGCGTGCCCGCTGACGTG; TTCCAGATCCAGGCGACCTCCGTCTACCCCGGTGCCTACAATGCCTTTCAGATCCGTGCT; GGAAACTCGCAGGGGGACTTTTACATTAGGCAAATCAACAACGTCAGCGCCATGCTGGTC; CTCGCCCGGCCGGTGACGGGCCCCCGGGAGTACGTGCTGGACCTGGAGATGGTCACCATG; AATTCCCTCATGAGCTACCGGGCCAGCTCTGTACTGAGGCTCACCGTCTTTGTAGGGGCC; TACACCTTCTGA; ; Clone variation with respect to NM_016938.4; 775 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016938 unedited; GGGGGNNGGGGGGNNNNNNNNTTTTNNNNNNNCGGTTCAGATTTGTATACAACTCACTAT; AGGCGGCCGCGCTGTGCCGTCCGCCCGTCCGTCTGCCCGCAGCATTGCCCGAGCCAGCCG; AGCCGCCAGAGCCGCGGGCCGCGGGGGTGTCGCGGGCCCAACCCCAGGAGCTCCCCTGCG; CCTCCTGCCTACCCGGGTCTCTACTGCTCTGGGCGCTGCTACTGTTGCTCTTGGGATCAG; CTTCTCCTCAGGATTCTGAAGAGCCCGACAGCTACACGGAATGCACAGATGGCTATGAGT; GGGACCCAGACAGCCAGCACTGCCGGGATGTCAACGAGTGTCTGACCATCCCTGAGGCCT; GCAAGGGGGAAATGAAGTGCATCAACCACTACGGGGGCTACTTGTGCCTGCCCCGCTCCG; CTGCCGTCATCAACGACCTACACGGCGAGGGACCCCCGCCACCAGTGCCTCCCGCTCAAC; ACCCCAACCCCTGCCCACCAGGCTATGAGCCCGACGATCAGGACAGCTGTGTGGATGTGG; ACGAGTGTGCCCAGGCCCTGCACGACTGTCGCCCCAGCCAGGACTGCCATAACTTGCCTG; GCTCCTATCAGTGCACCTGCCCTGATGGTTACCGCAAGATCGGGCCCGAGTGTGTGGACA; TAGACGAGTGCCGCTACCGCTACTGCCAGCACCGCTGCGTGAACCTGCCTGGCTCCTTCC; GCTGCCAGTGCGAGCCGGGCTTCCAGCTGGGGCCTAACACCGCTCCTGTGTTGATGTGAA; CGAGTGTGACATGGGGGCCCCATGCGAGCAGCGCTGCTTCAACTCCTATGGGACCTTCCT; GTGTCGCTGCCACCANGGCTATGAGCTGCATCGGGATGGCTTCTNCTGCANTGATATTGA; TGAGTGTAGCTACTCCAGCTACCTTTGTCAGTACCGCTGCGTTCACGAGCCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016938 unedited; CGTTGAGCCATTGGCGATGGTCACTTGCCAGGNCCAGNATAGCACTGGGGNAGGGTCACA; GGGCATGCCACCCGGGTATCTGTTGCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGA; GTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAGTCCATTTGCCTCGTTTTATA; AAAAAACAGGCCCAGGGAACTACTAGGGCTTATCCAAATGTACAGTTTGAGGCAGATTTA; GGAATGGAACCCAGGGCCTCCTGGCGCTGTCCAGAGTGGAGTTTCTTAAGACCCCTGCAA; GCCAGCCAAGTCCAAATCTCTGGGCCGGGGCCTGGAGTCCGCCCTCCTCGTTACCTCCTC; TCCTCTCGGGGTGACTGAAGCTCGTGGTGCAAAGCTCCCATCTCCTGTCAATGGGGGCCC; CTGGGCCTGAACATATAGAGACCCCCATTTAGGTGAACTTGGCCTGCCCCCCCAAGTGCC; ACCCTGCCCCAGCCTGAGGCTTCCTGCAGTGTGACCCGCCCACCTCAGCCACCAGGACTT; TCTTTCTCCCTTTATTGCCTTTCTCATTCTGCCCCTCACAACAGGCTCCTCATCTAGGGT; AACTGCAGGGAGGGTGGCTCCCTCCTGCTCCTCAGAAGGTGTAGGCCCCTACAAAGACGG; TGAGCCTCATTACAGATCTGGCCCGGTAGCTCATGAAGGAATTCATGGTGACCATCTCCA; GGTCCAGCACGTACTCCCGGAGGCCCGTCACCGGCCAGGCGAGGACCANCATGGCGCTGA; CGTTGTTGATTTGCCTAATGTAAAAGTCCCCCTGCGAGTTTCCATCCCGGATCTGAAAGC; ATTGTACGCCCCGGGGTAGACGGGAGTCGCCTGGATCTGGACACGTCAGCGGGCAGCTCC; GCTCCGAGGTGATGTCATGTTACCGTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_016938
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016938.1, NP_058634.1
• RefSeq Size: 1757 bp
• RefSeq ORF: 1332 bp
• Locus ID: 30008
• UniProt ID: O95967
• Cytogenetics: 11q13.1
• Domains: EGF, EGF_CA
• Protein Families: Druggable Genome, Secreted Protein
• Summary: A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein encoded by this gene contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jan 2011]; Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.