KLHL3 (NM_017415) Human Untagged Clone

CAT#: SC114103

KLHL3 (untagged)-Human kelch-like 3 (Drosophila) (KLHL3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KLHL3
• Synonyms: PHA2D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114103 sequence for NM_017415 edited (data generated by NextGen Sequencing)
  ATGGAGGGTGAAAGTGTCAAGCTGAGCTCCCAGACTCTGATACAGGCTGGGGATGATGAG
  AAGAACCAGAGGACGATCACTGTCAACCCTGCCCACATGGGGAAAGCATTCAAGGTTATG
  AATGAACTGCGGAGTAAACAGCTGTTGTGTGACGTGATGATTGTGGCAGAAGATGTCGAG
  ATAGAAGCCCACCGTGTGGTCCTGGCAGCCTGCAGCCCCTACTTCTGTGCGATGTTCACA
  GGTGACATGTCTGAGAGTAAAGCCAAAAAGATAGAAATCAAGGACGTGGATGGGCAGACG
  CTGAGTAAGCTGATTGACTACATCTATACTGCTGAAATCGAGGTGACTGAAGAGAATGTC
  CAGGTGCTGCTCCCGGCAGCCAGCTTGCTGCAGCTCATGGATGTTCGGCAGAACTGCTGT
  GACTTCCTGCAGTCTCAGTTGCATCCCACCAATTGCCTGGGCATCCGTGCATTTGCAGAT
  GTACACACCTGCACTGACCTTCTGCAGCAGGCCAATGCCTACGCAGAGCAGCACTTTCCA
  GAGGTGATGCTAGGAGAAGAATTTCTTAGCCTGAGTCTGGACCAGGTGTGCAGCTTGATA
  TCCAGCGACAAGCTGACCGTTTCTTCAGAAGAGAAGGTGTTTGAAGCTGTGATCTCATGG
  ATCAATTATGAGAAAGAAACCCGTTTAGAGCACATGGCAAAGCTGATGGAACATGTCCGA
  CTTCCTCTCTTACCTAGGGACTACCTAGTCCAAACGGTTGAAGAAGAAGCTTTGATAAAG
  AATAACAACACCTGTAAAGACTTCCTCATTGAGGCCATGAAATACCATCTCCTCCCTCTG
  GATCAGAGACTATTGATTAAGAACCCAAGGACCAAGCCCAGGACTCCAGTCAGCCTTCCC
  AAGGTCATGATTGTGGTTGGCGGCCAGGCACCCAAGGCAATCCGCAGTGTGGAGTGCTAT
  GATTTCGAGGAGGACCGGTGGGATCAGATTGCTGAGCTTCCTTCCAGAAGATGCAGAGCA
  GGTGTGGTGTTCATGGCTGGCCACGTGTATGCCGTGGGAGGGTTTAATGGCTCACTGCGG
  GTGCGGACAGTGGATGTGTATGACGGCGTGAAGGACCAGTGGACGTCCATTGCCAGCATG
  CAGGAGCGCCGGAGCACACTGGGCGCAGCGGTGCTCAATGACTTGCTCTACGCAGTGGGA
  GGCTTTGATGGCAGTACTGGCCTAGCATCGGTGGAAGCCTACAGCTACAAGACCAACGAG
  TGGTTCTTTGTGGCCCCGATGAACACGCGGCGGAGCAGTGTGGGTGTGGGCGTTGTGGAG
  GGGAAGCTATATGCTGTTGGGGGTTATGATGGAGCTTCCCGCCAGTGTCTGAGCACTGTG
  GAGCAGTACAACCCAGCGACCAATGAATGGATATACGTGGCGGACATGAGCACCCGCCGC
  AGTGGCGCAGGGGTTGGAGTGCTTAGCGGACAGCTGTACGCCACAGGTGGGCATGATGGG
  CCTTTGGTGAGGAAGAGCGTTGAGGTTTACGATCCTGGAACAAATACCTGGAAGCAAGTG
  GCAGACATGAACATGTGCCGGCGCAACGCAGGGGTCTGTGCAGTAAATGGGCTCCTGTAT
  GTGGTTGGAGGGGATGATGGATCCTGCAACTTGGCTTCGGTGGAGTACTACAATCCTGTC
  ACTGACAAATGGACGCTGCTTCCAACGAACATGAGCACGGGGCGGAGCTATGCAGGTGTT
  GCCGTGATTCACAAGTCCTTGTGA
  
  Clone variation with respect to NM_017415.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017415 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTC
  GGCACGAGGCGAGTGCATACACAGTTCAGCAGCTACCACCCTGGCTGGGCCTCACACAAT
  GGAGGGTGAAAGTGTCAAGCTGAGCTCCCAGACTCTGATACAGGCTGGGGATGATGAGAA
  GAACCAGAGGACGATCACTGTCAACCCTGCCCACATGGGGAAAGCATTCAAGGTTATGAA
  TGAACTGCGGAGTAAACAGCTGTTGTGTGACGTGATGATTGTGGCAGAAGATGTCGAGAT
  AGAAGCCCACCGTGTGGTCCTGGCAGCCTGCAGCCCCTACTTCTGTGCGATGTTCACAGG
  TGACATGTCTGAGAGTAAAGCCAAAAAGATAGAAATCAAGGACGTGGATGGGCAGACGCT
  GAGTAAGCTGATTGACTACATCTATACTGCTGAAATCGAGGTGACTGAAGAGAATGTCCA
  GGTGCTGCTCCCGGCAGCCAGCTTGCTGCAGCTCATGGATGTTCGGCAGAACTGCTGTGA
  CTTCCTGCAGTCTCAGTTGCATCCCACCAATTGCCTGNGCATCCGTGCATTTGCAGATGT
  ACACACCTGCACTGACCTTCTGCAGCAGGCCAATGCCTACGCAGAGCAGCACTTTCCAGA
  GGTGATGCTAGGAGAAGAATTTCTTACCCTGAGTCTGGACCAAGGGTGCAGCTTGATATC
  CACCGACAACCTGACCCGTTTCTCCAAAAGAAAAAGGTGCTTGAAGCTGTGATCTCATGG
  ATCAATATGAGAAAGAACCCGTTTAGAGCCATGGCAAAGCTGATGGACCATGTTCGACTT
  CCTCTTTTACCTAGGGACTACCTTATCCCAACGGTGGAGGAAGAACTTTGATAAAAAATA
  CCACCCCGGCAAGAGTTCCTATTGGAGCCATAAAATCCCTCTCCTCCTCTGAACACAGAC
  ATGGATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017415 unedited
  GTGGGCCAAGNNNNNNNNNAACTTTTTACTTGGACCCGGCCGCAATCTAGATCGAGTTTT
  TTTTTTTTTTTTTTGGTTTGGTTTGTTTTTAAGTGTTACCCGAGCTTCTCTGAAGACTAG
  TTTTCCCAGCTGAAGAGATATAACACCTCCCAGGCCTGGAATCTATGACATCAAAGACCA
  ATGGCCTAAACCTGAAGACTGGGCAGTTCTCATCTGCTTTCCTGTAGGGAGAACTCAAAG
  GAAAAACAGTGGAGCTTGAGAACCCCAGGGAACCCCTTTGGGCCCCTTTATCTAACCCCA
  CAAAAGGACCCGGGGCCACTTTTAAAAAATTTTTCCCCAATTTTTTTTGGAAACAAAGTC
  CCCCGGGTTTTTAAAAAGCCCCTAAAGGGGGGGGGGGGGCCCAAAAAACACCCCCCCTTC
  CCGGGGTTTTTTGGGGATCCCCAAAGGGCACGGGGCTCTTAAAAAAAAAAACCTTTTTTT
  TTTTTGGGGCCCCCCGGGGGAAACACCCAAAAAAAGGGGCCCCCCCCCTTTTTTTTTTCT
  CCCCCCGGGGGGGGGGGTTTTAAAAAAAAAATTGTGGGGGGTGGCCCCCCCCCCCCCCCC
  CCCCCTAAAAAAAAAAAAAAAAAAAAAAAGGGGTGTTTCTCCCCCCCCCCCCCCCCCCCC
  CCCCCCCCCCGGGTTTGTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017415
• Insert Size: 3340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017415.1, NP_059111.1
• RefSeq Size: 6485 bp
• RefSeq ORF: 1764 bp
• Locus ID: 26249
• UniProt ID: Q9UH77
• Cytogenetics: 5q31.2
• Domains: BTB, Kelch
• Gene Summary: This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (1; also known as KLHL3a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.