Apelin (APLN) (NM_017413) Human Untagged Clone

CAT#: SC114101

APLN (untagged)-Human apelin (APLN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Apelin
• Synonyms: APEL; XNPEP2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017413, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCTGCGGCTCTGCGTGCAGGCGCTCCTGCTGCTCTGGCTCTCCTTGACCGCGGTGTGTGGAGGGT
  CCCTGATGCCGCTTCCCGATGGGAATGGGCTGGAAGACGGCAATGTCCGCCACCTGGTGCAGCCCAGAGG
  GTCAAGGAATGGGCCAGGGCCCTGGCAGGGAGGTCGGAGGAAATTCCGCCGCCAGCGGCCCCGCCTCTCC
  CATAAGGGACCCATGCCTTTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017413 unedited
  CCCCCCATACCCCCGCCCGANGCCGCTTTGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT
  ATAAGCAGATCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
  GCCGCGAATTCGGCACGAGGGGCAGTTGCAGCCGCGGCCGAGCAGCCAGCCGCTAAGAAA
  GAGCTCGCCGCTGCCGCTCCCGGAGCCGCCGAGGCCAGCTTCGCGGCGCTGCCCCGCGGC
  GGGAGAGGAGGCTGCAGAAGAGCGGAGGCGGCCAGCGGGAGCGGCGGGGCTCAGCGCGCA
  CACTCAGCGGCCGGGGAGCCTCCCGAGCTCCGCGCCCGCACGCGCCAGCCGCGGCTCGCG
  CCTTTCTTGGCCTCCGGGCGCCCGACCTCTCCTCCCCCGCGCCGGCTCGCCGGGGCCGCG
  GCGGCCCAAGGAGCAGCATGAATCTGCGGCTCTGCGTGCAGGCGCTCCTGCTGCTCTGGC
  TCTCCTTGACCGCGGTGTGTGGAGGGTCCCTGATGCCGCTTCCCGATGGGAATGGGCTGG
  AAGACGGCAATGTCCGCCACCTGGTGCAGCCCAGAGGGTCAAGGAATGGGCCAGGGCCCT
  GGCAGGGAGGTCGGAGGAAATTCCGCCGCCAGCGGCCCCGCCTCTCCCATAAGGGACCCA
  TGCCTTTCTGAAGCAGGACTGAAGGGGCCCCCAAGTGCCCACCCCCTGCGGTTATGTCTC
  CTCCATAGAGTGGTCTGCTTCTCTGGAGGCCTCACGTCCATTCAGCTCTCACCTCGCACC
  TGCTGTAGCCACCAGTGGGCCCAGCTCTTCTCACCTGCCTGCTTCCCCAGTGGCGTGCTC
  CTGGCATGTAGTTTGGNATGAATCCCCGTCTCTCACAGAAATCCGTCCAGTCCATCATTC
  TGGCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017413 unedited
  TTTTTTAGATACTCTATGNNACCGCGGCCGCAATCTANNATCGAGTTTTTTTTTTTTTTT
  TTTATTATCAAATGTATTTATTGCTGAAAACATAACATTTTTCAAGAAAGGCAAACTGGC
  TAAAAAGTCCTGAAAGTGTTCAAAAGTAGATAAAATAGCAGAAGACACCCACCAAGGATA
  CAAAACAATTTTTAGTAGCGATCCTGCATTTAATCAGTATGTTCTTAAATAAACTGCTTT
  AAAAAAATTCTTCAAATGACACTGCCAAAAAAATTAGGACACCCAAACAGATGCCAGAAA
  ACCTGTAAGTGGGCTGGATTTTATGTGACCTGGTCATTAAGCATAGGGATTCATTTTGTG
  AGCTCATGGCAAATCAGTTTGAGGCCAGTTGACCTAGAACCGATTTGGGATGCAGGCACT
  TACCTCCCTGCACGTGCAATATGTGGGCATGGGGACACCCATGACCCCCAGGGGAAAGGA
  ATGCCCCTCCTGAACATGACCTCCAAGAGTAAGGGCGAACTGTCAGCTTTTAACTGTTTA
  TTATTAAGACATATTTACACAGAACAATCTTTACAAACATTGAACACAGGGGAAGGGGAC
  AATTTCTTAATGAACAGGGCCTATATATCTTTGTATAAAATAGTATAAGAATCATAAACC
  ACCCCTTTAAATAGGGCACCCCCCTTAGCCCACCCACTACACTTTTTTGTTCCCTAATCT
  CCCAATTTTCTTAAACATCCCCCCACTTTTCTCCCCTTTCCCAACGGGGCTTGGGCTTTG
  GCTTGCACGTTAATGGCAAGGTCCGTTGAGGCCGGGAGCACAACGGAAGGGGAGGGGGAA
  AAGAAAGGCCCAATCCCCTGGAGGGGGGCCGCATGGGGCGAAAGAGAATTTTTCGGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017413
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017413.3, NP_059109.3
• RefSeq Size: 3125 bp
• RefSeq ORF: 234 bp
• Locus ID: 8862
• UniProt ID: Q9ULZ1
• Cytogenetics: Xq26.1
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. [provided by RefSeq, Feb 2016]