AHI1 (NM_017651) Human Untagged Clone

CAT#: SC114030

AHI1 (untagged)-Human Abelson helper integration site 1 (AHI1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AHI1
• Synonyms: AHI-1; dJ71N10.1; JBTS3; ORF1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017651, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTACAGCTGAGAGTGAAGCAAAAGTAAAAACCAAAGTTCGCTTTGAAGAATTGCTTAAGACCCACA
  GTGATCTAATGCGTGAAAAGAAAAAACTGAAGAAAAAACTTGTCAGGTCTGAAGAAAACATCTCACCTGA
  CACTATTAGAAGCAATCTTCACTATATGAAAGAAACTACAAGTGATGATCCCGACACTATTAGAAGCAAT
  CTTCCCCATATTAAAGAAACTACAAGTGATGATGTAAGTGCTGCTAACACTAACAACCTGAAGAAGAGCA
  CGAGAGTCACTAAAAACAAATTGAGGAACACACAGTTAGCAACTGAAAATCCTAATGGTGATGCTAGTGT
  AGAGGAAGACAAACAAGGAAAGCCAAATAAAAAGGTGATAAAGACGGTGCCCCAGTTGACTACACAAGAC
  CTGAAACCGGAAACTCCTGAGAATAAGGTTGATTCTACACACCAGAAAACACATACAAAGCCACAGCCAG
  GCGTTGATCATCAGAAAAGTGAGAAGGCAAATGAGGGAAGAGAAGAGACTGATTTAGAAGAGGATGAAGA
  ATTGATGCAAGCATATCAGTGCCATGTAACTGAAGAAATGGCAAAGGAGATTAAGAGGAAAATAAGAAAG
  AAACTGAAAGAACAGTTGACTTACTTTCCCTCAGATACTTTATTCCATGATGACAAACTAAGCAGTGAAA
  AAAGGAAAAAGAAAAAGGAAGTTCCAGTCTTCTCTAAAGCTGAAACAAGTACATTGACCATCTCTGGTGA
  CACAGTTGAAGGTGAACAAAAGAAAGAATCTTCAGTTAGATCAGTTTCTTCAGATTCTCATCAAGATGAT
  GAAATAAGCTCAATGGAACAAAGCACAGAAGACAGCATGCAAGATGATACAAAACCTAAACCAAAAAAAA
  CAAAAAAGAAGACTAAAGCAGTTGCAGATAATAATGAAGATGTTGATGGTGATGGTGTTCATGAAATAAC
  AAGCCGAGATAGCCCGGTTTATCCCAAATGTTTGCTTGATGATGACCTTGTCTTGGGAGTTTACATTCAC
  CGAACTGATAGACTTAAGTCAGATTTTATGATTTCTCACCCAATGGTAAAAATTCATGTGGTTGATGAGC
  ATACTGGTCAATATGTCAAGAAAGATGATAGTGGACGGCCTGTTTCATCTTACTATGAAAAAGAGAATGT
  GGATTATATTCTTCCTATTATGACCCAGCCATATGATTTTAAACAGTTAAAATCAAGACTTCCAGAGTGG
  GAAGAACAAATTGTATTTAATGAAAATTTTCCCTATTTGCTTCGAGGCTCTGATGAGAGTCCTAAAGTCA
  TCCTGTTCTTTGAGATTCTTGATTTCTTAAGCGTGGATGAAATTAAGAATAATTCTGAGGTTCAAAACCA
  AGAATGTGGCTTTCGGAAAATTGCCTGGGCATTTCTTAAGCTTCTGGGAGCCAATGGAAATGCAAACATC
  AACTCAAAACTTCGCTTGCAGCTATATTACCCACCTACTAAGCCTCGATCCCCATTAAGTGTTGTTGAGG
  CATTTGAATGGTGGTCAAAATGTCCAAGAAATCATTACCCATCAACACTGTACGTAACTGTAAGAGGACT
  GAAAGTTCCAGACTGTATAAAGCCATCTTACCGCTCTATGATGGCTCTTCAGGAGGAAAAAGGTAAACCA
  GTGCATTGTGAACGTCACCATGAGTCAAGCTCAGTAGACACAGAACCTGGATTAGAAGAGTCAAAGGAAG
  TAATAAAGTGGAAACGACTCCCTGGGCAGGCTTGCCGTATCCCAAACAAACACCTCTTCTCACTAAATGC
  AGGAGAACGAGGATGTTTTTGTCTTGATTTCTCCCACAATGGAAGAATATTAGCAGCAGCTTGTGCCAGC
  CGGGATGGATATCCAATTATTTTATATGAAATTCCTTCTGGACGTTTCATGAGAGAATTGTGTGGCCACC
  TCAATATCATTTATGATCTTTCCTGGTCAAAAGATGATCACTACATCCTTACTTCATCATCTGATGGCAC
  TGCCAGGATATGGAAAAATGAAATAAACAATACAAATACTTTCAGAGTTTTACCTCATCCTTCTTTTGTT
  TACACGGCTAAATTCCATCCAGCTGTAAGAGAGCTAGTAGTTACAGGATGCTATGATTCCATGATACGGA
  TATGGAAAGTTGAGATGAGAGAAGATTCTGCCATATTGGTCCGACAGTTTGATGTTCACAAAAGTTTTAT
  CAACTCACTTTGTTTTGATACTGAAGGTCATCATATGTATTCAGGAGATTGTACAGGGGTGATTGTTGTT
  TGGAATACCTATGTCAAGATTAATGATTTGGAACATTCAGTGCACCACTGGACTATAAATAAGGAAATTA
  AAGAAACTGAGTTTAAGGGAATTCCAATAAGTTATTTGGAGATTCATCCCAATGGAAAACGTTTGTTAAT
  CCATACCAAAGACAGTACTTTGAGAATTATGGATCTCCGGATATTAGTAGCAAGGAAGTTTGTAGGAGCA
  GCAAATTATCGGGAGAAGATTCATAGTACTTTGACTCCATGTGGGACTTTTCTGTTTGCTGGAAGTGAGG
  ATGGTATAGTGTATGTTTGGAACCCAGAAACAGGAGAACAAGTAGCCATGTATTCTGACTTGCCATTCAA
  GTCACCCATTCGAGACATTTCTTATCATCCATTTGAAAATATGGTTGCATTCTGTGCATTTGGGCAAAAT
  GAGCCAATTCTTCTGTATATTTACGATTTCCATGTTGCCCAGCAGGAGGCTGAAATGTTCAAACGCTACA
  ATGGAACATTTCCATTACCTGGAATACACCAAAGTCAAGATGCCCTATGTACCTGTCCAAAACTACCCCA
  TCAAGGCTCTTTTCAGATTGATGAATTTGTCCACACTGAAAGTTCTTCAACGAAGATGCAGCTAGTAAAA
  CAGAGGCTTGAAACTGTCACAGAGGTGATACGTTCCTGTGCTGCAAAAGTCAACAAAAATCTCTCATTTA
  CTTCACCACCAGCAGTTTCCTCACAACAGTCTAAGTTAAAGCAGTCAAACATGCTGACCGCTCAAGAGAT
  TCTACATCAGTTTGGTTTCACTCAGACCGGGATTATCAGCATAGAAAGAAAGCCTTGTAACCATCAGGTA
  GATACAGCACCAACGGTAGTGGCTCTTTATGACTACACAGCGAATCGATCAGATGAACTAACCATCCATC
  GCGGAGACATTATCCGAGTGTTTTTCAAAGATAATGAAGACTGGTGGTATGGCAGCATAGGAAAGGGACA
  GGAAGGTTATTTTCCAGCTAATCATGTGGCTAGTGAAACACTGTATCAAGAACTGCCTCCTGAGATAAAG
  GAGCGATCCCCTCCTTTAAGCCCTGAGGAAAAAACTAAAATAGAAAAATCTCCAGCTCCTCAAAAGCAAT
  CAATCAATAAGAACAAGTCCCAGGACTTCAGACTAGGCTCAGAATCTATGACACATTCTGAAATGAGAAA
  AGAACAGAGCCATGAGGACCAAGGACACATAATGGATACACGGATGAGGAAGAACAAGCAAGCAGGCAGA
  AAAGTCACTCTAATAGAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017651 unedited
  AAGCTCGNNGATTTGTNAATACGNACTTTCTGATAGGGNNCGGCCGCGCAATTCGGCACG
  AGCGGGACGGGCGGTTCTGCGTACCCCTCTCCGATCCTGATTGAGTCAATGCTTTGCATT
  CTCAGTCCTCTGCATAAAGCTGAGAGATGCCTACAGCTGAGAGTGAAGCAAAAGTAAAAA
  CCAAAGTTCGCTTTGAAGAATTGCTTAAGACCCACAGTGATCTATTGTTTGAAAAGAAAA
  AACTGAAGAAAAAACTTGTCAGGTCTGAAGAAAACATCTCACCTGACACTATTAGAAGCA
  ATCTTCACTATATGAAAGAAACTACAAGTGATGATCCCGACACTATTAGAAGCAATCTTC
  CCCATATTAAAGAAACTACAAGTGATGATGTAAGTGCTGCTAACACTAACAACCTGAAGA
  AGAGCACGAGAGTCACTAAAAACAAATTGAGGAACACACAGTTAGCAACTGAAAATCCTA
  ATGGTGATGCTAGTGTAGAGGAAGACAAACAAGGAAAGCCAAATAAAAAGGTGATAAAGA
  CGGTGCCCCAGTTGACTACACAAGACCTGAAACCGGAAACTCCTGAGAATAAGGTTGATT
  CTACACACCAGAAAACACATACAAAGCCACAGCCAGGCGTTGATCATCAGANAAGTGAGA
  AGGCAAATGAGGGAAGAGAAGAGACTGATTTAGAAGAGGATGAAGAATTGATGCAAGCAT
  ATCAGTGCCATGTAACTGAAGAAATGGCAAAGGAGATTAAGAGGAANNNATAGANAGAAC
  TGANAGAACAGTTGACTTACTTNTCCTCAGATACTTTATTCCATGATNNGACAACTAGCA
  GTGANAAAAAGGGAAAAGAANAAAGGAGTTNCCCAGTCTCTCTAAAGCTGAAACAGTACA
  TTGACCATCTCTGGTGACACAGNTGAAGGTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017651 unedited
  NNAAATTTACTGTGNNACCGCGGCCGCTTNCTANGATCGAGTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTGCAGAAAAACAGGAATTTTTATTTTTAAATTATCAAGTGCATTCACAAGA
  CAGAAAAAAACAAGCTTGTACAAGAGTGACTCAAGCTGGCTGGGCCCAGCTGTACCTGAC
  ACACAGACTTATACATCCAGAACAATGAAAACTCAGTGCCATCAGAACAGACTTTTGGGC
  GGATGGCTGTAGGCTTCACCAGTTATCAGTGCCACTCAACACTTTCAAAAATGCCTTTTT
  TTTAAAGTTCTACACAGCGGGACATGTTTACCAATTTTTATGCCTCTACATGTGCAGGGA
  TAAGAGCCAAATATATTTTTATTAGAAGATATTTCACACTGGGTATACATCTGGGCAAAA
  TTTACACCTCAATGCCCAACATTGTTTTTCTCACTTCTTATTTTAATGGAATTGACTGTC
  TAATTTGGATAGGTCTTTCTAATTTTATATTAGTTTTCACTTTCTTGGGGTTAAATATTT
  TATCAGCAGACCTTATCACACACTTGAGAAAGGAGTCAAGAAAAGAACAAAAAATGAATA
  TCCCATTATAATATCAAACTTCAAAACATATAGTCTATAATAATGACACTATTATTCTGG
  AACTTAGAAAAAATAAATAATGCAATATAATAATGTAAAAATATTAAGATTAAAATAAAT
  ATTAAACAATGTAAGTCTACAAAATAGGTTTGTGCTTAAATATGAAAAGCCTTTTTTAAA
  AAGTAAAGACATTGGGAACATTGGTGCCAGTCCAACCCAAAATACTTTTTATTTTAGTCT
  ATTGAGATACTTTATGAATTGATCCTTAAAAACACAAATTTTAATTAGAAGTAAATTGGT
  TTCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017651
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017651.3, NP_060121.3
• RefSeq Size: 5564 bp
• RefSeq ORF: 3591 bp
• Locus ID: 54806
• UniProt ID: Q8N157
• Cytogenetics: 6q23.3
• Domains: SH3, WD40
• Gene Summary: This gene is apparently required for both cerebellar and cortical development in humans. This gene mutations cause specific forms of Joubert syndrome-related disorders. Joubert syndrome (JS) is a recessively inherited developmental brain disorder with several identified causative chromosomal loci. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
  Transcript Variant: This variant (2) differs in the 5', compared to variant 1. Variants 1-3 and 5 all encode the same isoform (a).