NALP2 (NLRP2) (NM_017852) Human Untagged Clone

SKU SC113899

NLRP2 (untagged)-Human NLR family, pyrin domain containing 2 (NLRP2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NALP2
• Synonyms: CLR19.9; NALP2; NBS1; PAN1; PYPAF2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017852 edited; GAATTCGGCACGAGGCTGCAGCCCTCATCTCCGCCGGCGAGTAGGGCCAGGTGTTGGGAG; CTCCCACGTGGGACAAGATGGTGTCTTCGGCGCAGATGGGCTTCAACCTGCAGGCTCTCC; TGGAGCAGCTCAGCCAGGATGAGTTGAGCAAGTTCAAGTATCTGATCACGACCTTCTCCC; TGGCACACGAGCTCCAGAAGATCCCCCACAAGGAGGTAGACAAGGCTGATGGGAAGCAAC; TGGTAGAAATCCTCACCACCCATTGTGACAGCTACTGGGTGGAGATGGCGAGCCTCCAGG; TCTTTGAAAAGATGCACCGAATGGATCTGTCTGAGAGAGCAAAGGATGAAGTCAGAGAAG; CAGCTTTGAAATCCTTTAATAAAAGGAAACCTCTATCATTAGGGATAACACGGAAAGAAC; GACCACCTCTAGACGTGGACGAAATGCTGGAGCGCTTCAAAACAGAAGCACAAGCGTTTA; CAGAAACGAAAGGAAATGTCATCTGCCTGGGTAAAGAAGTCTTTAAAGGAAAAAAGCCAG; ACAAAGACAATAGGTGCAGGTATATATTGAAGACGAAGTTCCGGGAGATGTGGAAGAGCT; GGCCTGGAGATAGCAAAGAGGTCCAGGTTATGGCTGAGAGATACAAGATGCTGATCCCAT; TCAGCAACCCCAGGGTGCTTCCCGGGCCCTTCTCATACACGGTGGTGCTGTATGGTCCTG; CAGGCCTTGGGAAAACCACGCTGGCCCAGAAACTAATGCTAGACTGGGCAGAGGACAACC; TCATCCACAAATTCAAATATGCGTTCTACCTCAGCTGCAGGGAGCTCAGCCGCCTGGGCC; CGTGCAGTTTTGCAGAGCTGGTCTTCAGGGACTGGCCTGAATTGCAGGATGACATTCCAC; ACATCCTAGCCCAAGCACGGAAAATCTTGTTCGTGATTGACGGCTTTGATGAGCTGGGAG; CCGCACCTGGGGCGCTGATCGAGGACATCTGCGGGGACTGGGAGAAGAAGAAGCCGGTGC; CCGTCCTCCTGGGGAGTTTGCTGAACAGGGTGATGTTACCCAAGGCCGCCCTGCTGGTCA; CCACGCGGCCCAGGGCCCTGAGGGACCTCCGGATCCTGGCGGAGGAGCCGATCTACATAA; GGGTGGAGGGCTTCCTGGAGGAGGACAGGAGGGCCTATTTCCTGAGACACTTTGGAGACG; AGGACCAAGCCATGCGTGCCTTTGAGCTAATGAGGAGCAACGCGGCCCTGTTCCAGCTGG; GCTCGGCCCCCGCGGTGTGCTGGATCGTGTGCACGACTCTGAAGCTGCAGATGGAGAAGG; GGGAGGACCCGGTCCCCACCTGCCTCACCCGCACGGGGCTGTTCCTGCGTTTCCTCTGCA; GCCGGTTCCCGCAGGGCGCACAGCTGCGGGGCGCGCTGCGGACGCTGAGCCTCCTGGCCG; CGCAGGGCCTGTGGGCGCAGACGTCCGTGCTTCACCGAGAGGATCTGGAAAGGCTCGGGG; TGCAGGAGTCCGACCTCCGTCTGTTCCTGGACGGAGACATCCTCCGCCAGGACAGAGTCT; CCAAAGGCTGCTACTCCTTCATCCACCTCAGCTTCCAGCAGTTTCTCACTGCCCTGTTCT; ACACCCTGGAGAAGGAGGAGGAAGAGGATAGGGACGGCCACACCTGGGACATTGGGGACG; TACAGAAGCTGCTTTCCGGAGTAGAAAGACTCAGGAACCCCGACCTGATCCAAGCAGGCT; ACTACTCCTTTGGCCTCGCTAACGAGAAGAGAGCCAAGGAGTTGGAGGCCACTTTTGGCT; GCCGGATGTCACCGGACATCAAACAGGAATTGCTGCGATGCGACATAAGTTGTAAGGGTG; GACATTCAACGGTGACAGACCTGCAGGAGCTCCTCGGCTGTCTGTACGAGTCTCAGGAGG; AGGAGCTGGTGAAGGAGGTGATGGCTCAGTTCAAAGAAATATCCCTGCACTTAAATGCAG; TAGACGTTGTGCCATCTTCATTCTGCGTCAAGCACTGTCGAAACCTGCAGAAAATGTCAC; TGCAGGTAATAAAGGAGAATCTCCCGGAGAATGTCACTGCGTCTGAATCAGACGCCGAGG; TTGAGAGATCCCAGGATGATCAGCACATGCTTCCTTTCTGGACGGACCTTTGTTCCATAT; TTGGATCAAATAAGGATCTGATGGGTCTAGCAATCAATGATAGCTTTCTCAGTGCCTCCC; TAGTAAGGATCCTGTGTGAACAAATAGCCTCTGACACCTGTCATCTCCAGAGAGTGGTGT; TCAAAAACATTTCCCCAGCTGATGCTCATCGGAACCTCTGCCTAGCTCTTCGAGGTCACA; AGACTGTAACGTATCTGACCCTTCAAGGCAATGACCAGGATGATATGTTTCCCGCATTGT; GTGAGGTCTTGAGACATCCAGAATGTAACCTGCGATATCTCGGGTTGGTGTCTTGTTCCG; CTACCACTCAGCAGTGGGCTGATCTCTCCTTGGCCCTTGAAGTCAACCAGTCCCTGACGT; GCGTAAACCTCTCCGACAATGAGCTTCTGGATGAGGGTGCTAAGTTGCTGTACACAACTT; TGAGACACCCCAAGTGCTTTCTGCAGAGGTTGTCGTTGGAAAACTGTCACCTTACAGAAG; CCAATTGCAAGGACCTTGCTGCTGTGTTGGTTGTCAGCCGGGAGCTGACACACCTGTGCT; TGGCCAAGAACCCCATTGGGAATACAGGGGTGAAGTTTCTGTGTGAGGGCTTGAGGTACC; CCGAGTGTAAACTGCAGACCTTGGTGCTTTGGAACTGCGACATAACTAGCGATGGCTGCT; GCGATCTCACAAAGCTTCTCCAAGAAAAATCAAGCCTGTTGTGTTTGGATCTGGGGCTGA; ATCACATAGGAGTTAAGGGAATGAAGTTCCTGTGTGAGGCTTTGAGGAAACCACTGTGCA; ACTTGAGATGTCTGTGGTTGTGGGGATGTTCCATCCCTCCGTTCAGTTGTGAAGACCTCT; GCTCTGCCCTCAGCTGCAACCAGAGCCTCGTCACTCTGGACCTGGGTCAGAATCCCTTGG; GGTCTAGTGGAGTGAAGATGCTGTTTGAAACCTTGACATGTTCCAGTGGCACCCTCCGGA; CACTCAGGTTGAAAATAGATGACTTTAATGATGAACTCAATAAGCTGCTGGAAGAAATAG; AAGAAAAAAACCCACAACTGATTATTGATACTGAGAAACATCATCCCTGGGCAGAAAGGC; CTTCTTCTCATGACTTCATGATCTGAATCCCCCCGAGTCATTCATTCTCCATGAAGTCAT; CGATTTTCCAGGTGTTGGTGAACTGCCTGTGACTCCTCTCCTCCCCGGCCCCTACCCCTC; AGGGATAATGAGTTCATTGCTGGGCTAGATGTTTTAGCCATGATTCTGCCTCTGTTTTAT; ACCTGCACACATCCTTATCTTTGTTACATATGAAATATCTGTATCACGGGTATATTGAGA; GAAATAAAGGTGAGAGCATTCACAAATGAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017852 unedited; TCGGTTTTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGCTGCAGCCCTCA; TCTCCGCCGGCGAGTAGGGCCAGGTGTTGGGAGCTCCCACGTGGGACAAGATGGTGTCTT; CGGCGCAGATGGGCTTCAACCTGCAGGCTCTCCTGGAGCAGCTCAGCCAGGATGAGTTGA; GCAAGTTCAAGTATCTGATCACGACCTTCTCCCTGGCACACGAGCTCCAGAAGATCCCCC; ACAAGGAGGTAGACAAGGCTGATGGGAAGCAACTGGTAGAAATCCTCACCACCCATTGTG; ACAGCTACTGGGTGGAGATGGCGAGCCTCCAGGTCTTTGAAAAGATGCACCGAATGGATC; TGTCTGAGAGAGCAAAGGATGAAGTCAGAGAAGCAGCTTTGAAATCCTTTAATAAAAGGA; AACCTCTATCATTAGGGATAACACGGAAAGAACGACCACCTCTAGACGTGGACGAAATGC; TGGAGCGCTTCAAAACAGAAGCACAAGCGTTTACAGAAACGAAAGGAAATGTCATCTGCC; TGGGTAAAGAAGTCTTTAAAGGAAAAAAGCCAGACAAAGACAATAGGTGCAGGTATATAT; TGAAGACGAAGTTCCGGGAGATGTGGAAGAGCTGGCCTGGAGATAGCAAAGAGGTCCAGG; TTATGGCTGAGAGATACAAGATGCTGATCCCATTCACACCCCCCAGGGTGCTTCCCGGGC; CCTTCTATACACGGTGGTGCTGTATGGTCCTGCNAGCCTTGGNGANACCACGCTGGCCCA; GAACTATGCTAGACTGGGCAGAGGGACACCTCATCCACAATTCAAATATGCGTTCTACCT; CAGCTGCAGGGAGCTCAGCCGCCTGGGCCCCGTGCAGTTTTGCCAGAGCTGGTCTTTCAG; GNACTGCNNCTGAATTGCAGNATGACATTCACC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017852 unedited; TTGCTTTGGACCGCGGGCCGCAATCTAGGGTCGAGTTTTTTTTTTTTTTTTTTCATTTGT; GAATGCTCTCACCTTTATTTCTCTCAATATACCCGTGATACAGATATTTCATATGTAACA; AAGATAAGGATGTGTGCAGGTATAAAACAGAGGCAGAATCATGGCTAAAACATCTAGCCC; AGCAATGAACTCATTATCCCTGAGGGGTAGGGGCCGGGGAGGAGAGGAGTCACAGGCAGT; TCACCAACACCTGGAAAATCGATGACTTCATGGAGAATGAATGACTCGGGGGGATTCAGA; TCATGAAGTCATGAGAAGAAGGCCTTTCTGCCCAGGGATGATGTTTCTCAGTATCAATAA; TCAGTTGGGGGTTTTTTTCTTCTATTTCTTCCAGCAGCTTATTGAGTTCATCATTAAAGT; CATCTATTTTCAACCTGAGTGTCCGGAGGGTGCCACTGGAACATGTCAAGGTTTCAAACA; GCATCTTCACTCCACTAGACCCCAAGGGATTCTGACCCAGGTCCAGAGTGACGAGGCTCT; GGTTGCAGCTGAGGGCAGAGCAGAGGTCTTCACAACTGAACGGAGGGATGGAACATCCCC; ACAACCACAGACATCTCAAGTTGCACAGTGGTTTCCTCAAAGCCTCACACAGGAACTTCA; TTCCCTTAACTCCTATGTGATTCAGCCCCAGATCCAAACACAACAGGCTTGATTTTTCTT; GGAGAAGCTTTGTGAGATCGCAGCAGCCATCGCTAGTTATGTCGCAGTTCCAAAGCACCA; AGGTCTGCAGTTTACACTCGGGTACCTCAAGCCCTCCACAGAAACTTCACCCCTGTATTN; CAATGGGGNTNCTGGNCCAAAGCACAGTGTGNTCAGCTCCCGGCTGACAACCAACACAGC; AGCAGGTCCTTGCAATGGNCTTCTGTANGGTGACAGNNTTTCAACGACAACCTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_017852
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017852.1, NP_060322.1
• RefSeq Size: 3531 bp
• RefSeq ORF: 3189 bp
• Locus ID: 55655
• UniProt ID: Q9NX02
• Cytogenetics: 19q13.42
• Domains: LRR, LRR_RI
• Summary: This gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). Members of this gene family are thought to be important regulators of immune responses. This gene product interacts with components of the IkB kinase (IKK) complex, and can regulate both caspase-1 and NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity. The pyrin domain is necessary and sufficient for suppression of NF-kB activity. An allelic variant (rs147585490) has been found that is incapable of blocking the transcriptional activity of NF-kB. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]; Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1).