NALP2 (NLRP2) (NM_017852) Human Untagged Clone

CAT#: SC113899

NLRP2 (untagged)-Human NLR family, pyrin domain containing 2 (NLRP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NALP2
• Synonyms: CLR19.9; NALP2; NBS1; PAN1; PYPAF2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017852 edited
  GAATTCGGCACGAGGCTGCAGCCCTCATCTCCGCCGGCGAGTAGGGCCAGGTGTTGGGAG
  CTCCCACGTGGGACAAGATGGTGTCTTCGGCGCAGATGGGCTTCAACCTGCAGGCTCTCC
  TGGAGCAGCTCAGCCAGGATGAGTTGAGCAAGTTCAAGTATCTGATCACGACCTTCTCCC
  TGGCACACGAGCTCCAGAAGATCCCCCACAAGGAGGTAGACAAGGCTGATGGGAAGCAAC
  TGGTAGAAATCCTCACCACCCATTGTGACAGCTACTGGGTGGAGATGGCGAGCCTCCAGG
  TCTTTGAAAAGATGCACCGAATGGATCTGTCTGAGAGAGCAAAGGATGAAGTCAGAGAAG
  CAGCTTTGAAATCCTTTAATAAAAGGAAACCTCTATCATTAGGGATAACACGGAAAGAAC
  GACCACCTCTAGACGTGGACGAAATGCTGGAGCGCTTCAAAACAGAAGCACAAGCGTTTA
  CAGAAACGAAAGGAAATGTCATCTGCCTGGGTAAAGAAGTCTTTAAAGGAAAAAAGCCAG
  ACAAAGACAATAGGTGCAGGTATATATTGAAGACGAAGTTCCGGGAGATGTGGAAGAGCT
  GGCCTGGAGATAGCAAAGAGGTCCAGGTTATGGCTGAGAGATACAAGATGCTGATCCCAT
  TCAGCAACCCCAGGGTGCTTCCCGGGCCCTTCTCATACACGGTGGTGCTGTATGGTCCTG
  CAGGCCTTGGGAAAACCACGCTGGCCCAGAAACTAATGCTAGACTGGGCAGAGGACAACC
  TCATCCACAAATTCAAATATGCGTTCTACCTCAGCTGCAGGGAGCTCAGCCGCCTGGGCC
  CGTGCAGTTTTGCAGAGCTGGTCTTCAGGGACTGGCCTGAATTGCAGGATGACATTCCAC
  ACATCCTAGCCCAAGCACGGAAAATCTTGTTCGTGATTGACGGCTTTGATGAGCTGGGAG
  CCGCACCTGGGGCGCTGATCGAGGACATCTGCGGGGACTGGGAGAAGAAGAAGCCGGTGC
  CCGTCCTCCTGGGGAGTTTGCTGAACAGGGTGATGTTACCCAAGGCCGCCCTGCTGGTCA
  CCACGCGGCCCAGGGCCCTGAGGGACCTCCGGATCCTGGCGGAGGAGCCGATCTACATAA
  GGGTGGAGGGCTTCCTGGAGGAGGACAGGAGGGCCTATTTCCTGAGACACTTTGGAGACG
  AGGACCAAGCCATGCGTGCCTTTGAGCTAATGAGGAGCAACGCGGCCCTGTTCCAGCTGG
  GCTCGGCCCCCGCGGTGTGCTGGATCGTGTGCACGACTCTGAAGCTGCAGATGGAGAAGG
  GGGAGGACCCGGTCCCCACCTGCCTCACCCGCACGGGGCTGTTCCTGCGTTTCCTCTGCA
  GCCGGTTCCCGCAGGGCGCACAGCTGCGGGGCGCGCTGCGGACGCTGAGCCTCCTGGCCG
  CGCAGGGCCTGTGGGCGCAGACGTCCGTGCTTCACCGAGAGGATCTGGAAAGGCTCGGGG
  TGCAGGAGTCCGACCTCCGTCTGTTCCTGGACGGAGACATCCTCCGCCAGGACAGAGTCT
  CCAAAGGCTGCTACTCCTTCATCCACCTCAGCTTCCAGCAGTTTCTCACTGCCCTGTTCT
  ACACCCTGGAGAAGGAGGAGGAAGAGGATAGGGACGGCCACACCTGGGACATTGGGGACG
  TACAGAAGCTGCTTTCCGGAGTAGAAAGACTCAGGAACCCCGACCTGATCCAAGCAGGCT
  ACTACTCCTTTGGCCTCGCTAACGAGAAGAGAGCCAAGGAGTTGGAGGCCACTTTTGGCT
  GCCGGATGTCACCGGACATCAAACAGGAATTGCTGCGATGCGACATAAGTTGTAAGGGTG
  GACATTCAACGGTGACAGACCTGCAGGAGCTCCTCGGCTGTCTGTACGAGTCTCAGGAGG
  AGGAGCTGGTGAAGGAGGTGATGGCTCAGTTCAAAGAAATATCCCTGCACTTAAATGCAG
  TAGACGTTGTGCCATCTTCATTCTGCGTCAAGCACTGTCGAAACCTGCAGAAAATGTCAC
  TGCAGGTAATAAAGGAGAATCTCCCGGAGAATGTCACTGCGTCTGAATCAGACGCCGAGG
  TTGAGAGATCCCAGGATGATCAGCACATGCTTCCTTTCTGGACGGACCTTTGTTCCATAT
  TTGGATCAAATAAGGATCTGATGGGTCTAGCAATCAATGATAGCTTTCTCAGTGCCTCCC
  TAGTAAGGATCCTGTGTGAACAAATAGCCTCTGACACCTGTCATCTCCAGAGAGTGGTGT
  TCAAAAACATTTCCCCAGCTGATGCTCATCGGAACCTCTGCCTAGCTCTTCGAGGTCACA
  AGACTGTAACGTATCTGACCCTTCAAGGCAATGACCAGGATGATATGTTTCCCGCATTGT
  GTGAGGTCTTGAGACATCCAGAATGTAACCTGCGATATCTCGGGTTGGTGTCTTGTTCCG
  CTACCACTCAGCAGTGGGCTGATCTCTCCTTGGCCCTTGAAGTCAACCAGTCCCTGACGT
  GCGTAAACCTCTCCGACAATGAGCTTCTGGATGAGGGTGCTAAGTTGCTGTACACAACTT
  TGAGACACCCCAAGTGCTTTCTGCAGAGGTTGTCGTTGGAAAACTGTCACCTTACAGAAG
  CCAATTGCAAGGACCTTGCTGCTGTGTTGGTTGTCAGCCGGGAGCTGACACACCTGTGCT
  TGGCCAAGAACCCCATTGGGAATACAGGGGTGAAGTTTCTGTGTGAGGGCTTGAGGTACC
  CCGAGTGTAAACTGCAGACCTTGGTGCTTTGGAACTGCGACATAACTAGCGATGGCTGCT
  GCGATCTCACAAAGCTTCTCCAAGAAAAATCAAGCCTGTTGTGTTTGGATCTGGGGCTGA
  ATCACATAGGAGTTAAGGGAATGAAGTTCCTGTGTGAGGCTTTGAGGAAACCACTGTGCA
  ACTTGAGATGTCTGTGGTTGTGGGGATGTTCCATCCCTCCGTTCAGTTGTGAAGACCTCT
  GCTCTGCCCTCAGCTGCAACCAGAGCCTCGTCACTCTGGACCTGGGTCAGAATCCCTTGG
  GGTCTAGTGGAGTGAAGATGCTGTTTGAAACCTTGACATGTTCCAGTGGCACCCTCCGGA
  CACTCAGGTTGAAAATAGATGACTTTAATGATGAACTCAATAAGCTGCTGGAAGAAATAG
  AAGAAAAAAACCCACAACTGATTATTGATACTGAGAAACATCATCCCTGGGCAGAAAGGC
  CTTCTTCTCATGACTTCATGATCTGAATCCCCCCGAGTCATTCATTCTCCATGAAGTCAT
  CGATTTTCCAGGTGTTGGTGAACTGCCTGTGACTCCTCTCCTCCCCGGCCCCTACCCCTC
  AGGGATAATGAGTTCATTGCTGGGCTAGATGTTTTAGCCATGATTCTGCCTCTGTTTTAT
  ACCTGCACACATCCTTATCTTTGTTACATATGAAATATCTGTATCACGGGTATATTGAGA
  GAAATAAAGGTGAGAGCATTCACAAATGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017852 unedited
  TCGGTTTTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGCTGCAGCCCTCA
  TCTCCGCCGGCGAGTAGGGCCAGGTGTTGGGAGCTCCCACGTGGGACAAGATGGTGTCTT
  CGGCGCAGATGGGCTTCAACCTGCAGGCTCTCCTGGAGCAGCTCAGCCAGGATGAGTTGA
  GCAAGTTCAAGTATCTGATCACGACCTTCTCCCTGGCACACGAGCTCCAGAAGATCCCCC
  ACAAGGAGGTAGACAAGGCTGATGGGAAGCAACTGGTAGAAATCCTCACCACCCATTGTG
  ACAGCTACTGGGTGGAGATGGCGAGCCTCCAGGTCTTTGAAAAGATGCACCGAATGGATC
  TGTCTGAGAGAGCAAAGGATGAAGTCAGAGAAGCAGCTTTGAAATCCTTTAATAAAAGGA
  AACCTCTATCATTAGGGATAACACGGAAAGAACGACCACCTCTAGACGTGGACGAAATGC
  TGGAGCGCTTCAAAACAGAAGCACAAGCGTTTACAGAAACGAAAGGAAATGTCATCTGCC
  TGGGTAAAGAAGTCTTTAAAGGAAAAAAGCCAGACAAAGACAATAGGTGCAGGTATATAT
  TGAAGACGAAGTTCCGGGAGATGTGGAAGAGCTGGCCTGGAGATAGCAAAGAGGTCCAGG
  TTATGGCTGAGAGATACAAGATGCTGATCCCATTCACACCCCCCAGGGTGCTTCCCGGGC
  CCTTCTATACACGGTGGTGCTGTATGGTCCTGCNAGCCTTGGNGANACCACGCTGGCCCA
  GAACTATGCTAGACTGGGCAGAGGGACACCTCATCCACAATTCAAATATGCGTTCTACCT
  CAGCTGCAGGGAGCTCAGCCGCCTGGGCCCCGTGCAGTTTTGCCAGAGCTGGTCTTTCAG
  GNACTGCNNCTGAATTGCAGNATGACATTCACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017852 unedited
  TTGCTTTGGACCGCGGGCCGCAATCTAGGGTCGAGTTTTTTTTTTTTTTTTTTCATTTGT
  GAATGCTCTCACCTTTATTTCTCTCAATATACCCGTGATACAGATATTTCATATGTAACA
  AAGATAAGGATGTGTGCAGGTATAAAACAGAGGCAGAATCATGGCTAAAACATCTAGCCC
  AGCAATGAACTCATTATCCCTGAGGGGTAGGGGCCGGGGAGGAGAGGAGTCACAGGCAGT
  TCACCAACACCTGGAAAATCGATGACTTCATGGAGAATGAATGACTCGGGGGGATTCAGA
  TCATGAAGTCATGAGAAGAAGGCCTTTCTGCCCAGGGATGATGTTTCTCAGTATCAATAA
  TCAGTTGGGGGTTTTTTTCTTCTATTTCTTCCAGCAGCTTATTGAGTTCATCATTAAAGT
  CATCTATTTTCAACCTGAGTGTCCGGAGGGTGCCACTGGAACATGTCAAGGTTTCAAACA
  GCATCTTCACTCCACTAGACCCCAAGGGATTCTGACCCAGGTCCAGAGTGACGAGGCTCT
  GGTTGCAGCTGAGGGCAGAGCAGAGGTCTTCACAACTGAACGGAGGGATGGAACATCCCC
  ACAACCACAGACATCTCAAGTTGCACAGTGGTTTCCTCAAAGCCTCACACAGGAACTTCA
  TTCCCTTAACTCCTATGTGATTCAGCCCCAGATCCAAACACAACAGGCTTGATTTTTCTT
  GGAGAAGCTTTGTGAGATCGCAGCAGCCATCGCTAGTTATGTCGCAGTTCCAAAGCACCA
  AGGTCTGCAGTTTACACTCGGGTACCTCAAGCCCTCCACAGAAACTTCACCCCTGTATTN
  CAATGGGGNTNCTGGNCCAAAGCACAGTGTGNTCAGCTCCCGGCTGACAACCAACACAGC
  AGCAGGTCCTTGCAATGGNCTTCTGTANGGTGACAGNNTTTCAACGACAACCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017852
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017852.1, NP_060322.1
• RefSeq Size: 3531 bp
• RefSeq ORF: 3189 bp
• Locus ID: 55655
• UniProt ID: Q9NX02
• Cytogenetics: 19q13.42
• Domains: LRR, LRR_RI
• Gene Summary: This gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). Members of this gene family are thought to be important regulators of immune responses. This gene product interacts with components of the IkB kinase (IKK) complex, and can regulate both caspase-1 and NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity. The pyrin domain is necessary and sufficient for suppression of NF-kB activity. An allelic variant (rs147585490) has been found that is incapable of blocking the transcriptional activity of NF-kB. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1).