ASF1B (NM_018154) Human Untagged Clone

CAT#: SC113719

ASF1B (untagged)-Human ASF1 anti-silencing function 1 homolog B (S. cerevisiae) (ASF1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASF1B
• Synonyms: CIA-II
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113719 sequence for NM_018154 edited (data generated by NextGen Sequencing)
  ATGGCCAAGGTGTCGGTGCTGAACGTGGCGGTCCTGGAGAACCCGAGCCCTTTCCACAGC
  CCCTTCCGGTTCGAGATCAGCTTCGAGTGCAGTGAAGCCCTGGCGGACGACCTGGAGTGG
  AAGATCATTTATGTTGGCTCGGCTGAGAGTGAGGAATTTGATCAGATCCTAGACTCGGTG
  CTGGTGGGCCCTGTGCCAGCAGGGAGACACATGTTTGTCTTTCAGGCCGACGCCCCCAAC
  CCATCCCTCATCCCAGAGACTGATGCCGTGGGTGTGACTGTGGTCCTCATCACCTGCACC
  TACCATGGACAGGAGTTCATCCGAGTGGGCTACTACGTCAACAACGAGTACCTCAACCCT
  GAGCTGCGTGAGAACCCGCCCATGAAGCCAGATTTCTCCCAGCTCCAGCGGAACATCTTG
  GCCTCGAACCCCCGGGTGACCCGCTTCCATATCAACTGGGACAACAACATGGACAGGCTG
  GAGGCCATAGAGACCCAGGACCCCTCCCTGGGCTGCGGCCTCCCACTCAACTGCACTCCT
  ATCAAGGGCTTGGGGCTCCCTGGCTGCATCCCTGGCCTCCTCCCTGAGAACTCCATGGAC
  TGCATCTAA
  
  Clone variation with respect to NM_018154.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018154 unedited
  GCGTTCAGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGGTCAG
  CCCTACCCCACTGACCCCACCGGACCCAGGCGCGGCCTCCGCCACAGCCACAGCCCCTGC
  CCCTGCTGCGGCGCGGCGAGGCGAGGCGATGGCCAAGGTGTCGGTGCTGAACGTGGCGGT
  CCTGGAGAACCCGAGCCCTTTCCACAGCCCCTTCCGGTTCGAGATCAGCTTCGAGTGCAG
  TGAAGCCCTGGCGGACGACCTGGAGTGGAAGATCATTTATGTTGGCTCGGCTGAGAGTGA
  GGAATTTGATCAGATCCTAGACTCGGTGCTGGTGGGCCCTGTGCCAGCAGGGAGACACAT
  GTTTGTCTTTCAGGCCGACGCCCCCAACCCATCCCTCATCCCAGAGACTGATGCCGTGGG
  TGTGACTGTGGTCCTCATCACCTGCACCTACCATGGACAGGAGTTCATCCGAGTGGGCTA
  CTACGTCAACAACGAGTACCTCAACCCTGAGCTGCGTGAGAACCCGCCCATGAAGCCAGA
  TTTCTCCCAGCTCCAGCGGAACATCTTGGCCTCGAACCCCCGGGTGACCCGCTTCCATAT
  CAACTGGGACAACAACATGGACAGGCTGGAGGCCATAGAGACCCAGGACCCCTCCCTGGG
  CTGCGGCCTCCCACTCAACTGCACTCCTATCAAGGGCTTGGGGCTCCCTGGCTGCATCCC
  TGGCCTCCTCCCTGAGAACTCCATGGACTGCATCTAACTGCANGAACCCAGAGTGTCCCA
  GCACGCCGGGAGGGGCAACCCAGCCTNCCAGCGAGTCCTGCAGGGCCCATCTAGAGGACT
  TTGGGGGCCATCAGCTGCAATCCAGGTCTGTCAAACTCAGCCCCCCTAGAAAGACAGGCC
  TTTGGGTCTNCCCTAGTCCTGGCCAGAAGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018154 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTAAATGAAGTGCTTTTAATTTT
  CAGACCAAACATTTTTAATATAAAAACATTTTGATAATATACAAACAGCAATCACAACAG
  CATCCACATGGCAGCAAGGGGACCAGGGCACAGAGAGGGGGAGCGGGCTGGGGAGGGACA
  GTTTTCAGGGTCCCAGTTGCTTCCCTGGCTTGAAATCACCCTGGTCCTAGCAGAGGACAG
  GTTAAGGCTGCCAGAGGCAGAGGGTCCCTGACCCTGGCCCGGAGACAGACTGCCCAGGCA
  GGCCCTCTGATACCATCTTCCAACCATGGCAGCCTCCAGGAAAAGCCAGATCCATTTAGG
  AGATAACAGGAAGGTGGCTGTGATTGACAGGAAAGGCAACATGGTTCCTCAGCATCCTGC
  TGATCACACCTCTGGGAGGGGCTGCTGGATTGAAGAGGACCTAAGAATCTTCCTGGGAGC
  AGGACAGAAATGGGATCTAAGTCTACCTACTAACTAACATTCCCGCCTGTGACACCAGAA
  AGCTGGAGTGGGTCTGCTCAGATGTACAGGAAGAGAAAGAATGACAAAGCCTTTGCTCAG
  CAGGACTGGAGCCTTGACAGGCACTGACCANNGAAAGCTCNNTAGTGGCCAGGGAGGTCT
  GTGGAANNAGCCTTGATCACTGGTACTCAAGGGTGCACAGTGGCACAGTGGCCCTAGNTA
  GATTNATGAAGACGAAAAGACCAAGTCAGAATTAGAAACTGAGTACCTGCTTCTTATAGG
  CCTGTAGAGGAAAGCGAGATCTTCTCTTGCCAGACTAGGGAGAACCAGGGCTGTTCTTCC
  TAGGGCTGAATTGAAGAACTGTATTGACTGATGCCCCCAAGTCTTTAATGGCCTGCAGAC
  TGCTGGNAGCCTGTTGCCCTCCGGGGGCTGGAACTTTGGTCTGGGTTAAAGCACCCTGGA
  TTTCAGGAGAGGCAGGTGCACCGGGACCCCAACTTGATGATGCNTGATGGAAGCCACCAC
  GAGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018154
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018154.2, NP_060624.1
• RefSeq Size: 1746 bp
• RefSeq ORF: 609 bp
• Locus ID: 55723
• UniProt ID: Q9NVP2
• Cytogenetics: 19p13.12
• Domains: Anti-silence
• Gene Summary: This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008]