VPS33B (NM_018668) Human Untagged Clone

SKU SC113391

VPS33B (untagged)-Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: VPS33B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113391 sequence for NM_018668 edited (data generated by NextGen Sequencing); ATGGCTTTTCCCCATCGGCCGGACGCCCCTGAGCTGCCTGACTTCTCCATGCTGAAGAGG; CTGGCTCGAGACCAGCTCATCTATCTGCTGGAGCAGCTTCCTGGAAAAAAGGATTTATTC; ATTGAGGCAGATCTCATGAGCCCTTTGGATCGAATTGCCAATGTCTCCATCCTGAAGCAA; CACGAAGTAGACAAGCTATACAAGGTGGAGAACAAGCCAGCCCTCAGCTCCAATGAACAA; TTGTGCTTCTTGGTCAGACCCCGCATCAAGAATATGCGATACATTGCCAGTCTTGTCAAT; GCTGACAAATTGGCTGGCCGAACTCGCAAATACAAAGTGATCTTCAGCCCTCAAAAGTTC; TATGCGTGTGAGATGGTGCTTGAGGAAGAGGGAATCTATGGAGATGTGAGCTGTGATGAA; TGGGCCTTCTCTTTGCTGCCTCTTGATGTGGATCTGCTGAGCATGGAACTACCAGAATTT; TTCAGGGATTACTTTCTGGAAGGAGATCAGCGTTGGATCAACACTGTAGCTCAGGCCTTA; CACCTTCTCAGCACTCTCTATGGACCCTTTCCAAACTGCTATGGAATTGGCAGGTGCGCC; AAGATGGCATATGAATTGTGGAGGAACCTGGAGGAGGAGGAGGATGGCGAAACCAAGGGC; CGAAGGCCAGAGATTGGACATATCTTTCTCTTGGACAGAGATGTGGACTTTGTGACAGCA; CTTTGCTCCCAAGTGGTTTATGAGGGCCTAGTAGATGACACCTTCCGCATCAAGTGTGGG; AGTGTCGACTTTGGCCCAGAAGTCACATCCTCTGACAAGAGCCTGAAGGTGCTACTCAAT; GCCGAGGACAAGGTGTTTAATGAGATTCGGAACGAGCACTTCTCCAATGTCTTTGGCTTC; TTGAGCCAGAAGGCCCGGAACTTGCAGGCCCAGTATGATCGCCGGAGAGGCATGGACATT; AAGCAGATGAAGAATTTCGTGTCCCAGGAGCTCAAGGGCCTGAAACAGGAGCACCGCCTG; CTGAGTCTCCATATTGGGGCCTGTGAATCCATCATGAAGAAGAAAACCAAGCAGGATTTC; CAGGAGCTAATCAAGACTGAGCATGCACTGCTAGAGGGGTTCAACATCCGGGAGAGCACC; AGCTACATTGAGGAACACATAGACCGGCAGGTGTCGCCTATAGAAAGCCTGCGCCTCATG; TGCCTTTTGTCCATCACTGAGAATGGTTTGATCCCCAAGGATTACCGATCTCTGAAAACA; CAGTATCTGCAGAGCTATGGCCCTGAGCACCTGCTAACCTTCTCCAATCTGCGAAGAGCT; GGGCTCCTAACGGAGCAGGCCCCCGGGGACACCCTCACAGCCGTGGAGAGTAAAGTGAGC; AAGCTGGTGACCGACAAGGCTGCAGGAAAGATTACTGATGCCTTCAGTTCTCTGGCCAAG; AGGAGCAATTTTCGTGCCATCAGCAAAAAGCTGAATTTGATCCCACGTGTGGACGGCGAG; TATGATCTGAAAGTGCCCCGAGACATGGCTTACGTCTTCGGTGGTGCTTATGTGCCCCTG; AGCTGCCGAATCATTGAGCAGGTGCTAGAGCGGCGAAGCTGGCAGGGCCTTGATGAGGTG; GTACGGCTGCTCAACTGCAGTGACTTTGCATTCACAGATATGACTAAGGAAGACAAGGCT; TCCAGTGAGTCCCTGCGCCTCATCTTGGTGGTGTTCTTGGGTGGTTGTACATTCTCTGAG; ATCTCAGCCCTCCGGTTCCTGGGCAGAGAGAAAGGCTACAGGTTCATTTTCCTGACGACA; GCAGTCACAAACAGCGCTCGCCTTATGGAGGCCATGAGTGAGGTGAAAGCCTGA; ; Clone variation with respect to NM_018668.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018668 unedited; GTCGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGAGACAGGC; TGCTCCGAGGTAGTAGCTCTCTTGCCTGGAGGTGGCCATTCATTCCTGGAGTGCTGCTGA; GGAGCGAGGGCCCATCTGGGGTCTCTGGAAGTCGGTGCCCAGGCCTGAAGGATAGCCCCC; CTTGCGCTTCCCTGGGCTGCGGCCGGCCTTCTCAGAACGAAGGGCGTCCTTCCACCCCGC; GGCGCAGGTGACCGCTGCCATGGCTTTTCCCCATCGGCCGGACGCCCCTGAGCTGCCTGA; CTTCTCCATGCTGAAGAGGCTGGCTCGAGACCAGCTCATCTATCTGCTGGAGCAGCTTCC; TGGAAAAAAGGATTTATTCATTGAGGCAGATCTCATGAGCCCTTTGGATCGAATTGCCAA; TGTCTCCATCCTGAAGCAACACGAAGTAGACAAGCTATACAAGGTGGAGAACAAGCCAGC; CCTCAGCTCCAATGAACAATTGTGCTTCTTGGTCAGACCCCGCATCAAGAATATGCGATA; CATTGCCAGTCTTGTCAATGCTGACAAATTGGCTGGCCGAACTCGCAAATACAAAGTGAT; CTTCAGCCCTCAAAAGTTCTATGCGTGTGAGATGGTGCTTGAGGAAGAGGGAATCTATGG; AGATGTGAGCTGTGATGAATGGGCCTTCTCTTTGCTGCCTCTTGATGTGGATCTGCTGAG; CATGGAACTACCAGAATTTTTCAGGGATTACTTTCTGGAAGGAGATCAGCGTTGGATCAA; CACTGTAGCTCAAGCCTTACACCTTCTCAGCACTCTCTATGGACCCTNTCCAACTGCTAT; GGAATTGGCAGGTGCGCCAAGATGGCATATGAAANTGTGGAGGAACCTGNNNAGAGNAGG; AGGATGGCGAAACCCAGGGCCGAAGCCAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018668 unedited; ACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTCTGAAAGCAAAGGCTTTATTTACAATGACATTCAAACAGGATTTAA; CAAAGGATGCCTTTTCCTGCTCGCATTTTAACAGCATGGGTTGTACTTCATAAACAAAAA; AAAAAAATTTCCTGGGAGCAGGAAGGGAACTCTTTTCTCAAATAATGTTCTTTAAATCCC; AACACGTTCCATGCTCCCGGTTTTTAACAGGTAGTTGGGGGACACTTGGTTATAACAGCT; GGGTGCCAAATGCCTGCATCTCACTGAGGAATGGGTTCAGGGAAAATGTCAACACTGGCC; GGGAAAAACATCAGGCTTTCACCTCACTCATGGCCTCCATAAGGCGAGCCCTGTTTGTGA; CTGCTGTCGTCAGGAAAATGAACCTGTAGCCTTTTTTTTTGCCCAGGAACCGGAGGGCTG; AAATCTCAAAAAATGTCCAACCACCCAAAAACACCACCAAGATGAGGCGCAGGGACTCAC; TGGAAGCCTTGTCTTCCTTAGTCATATCTGGGAATGCAAAGTCACTGCAGTTGAGCAGCC; GTACCACCTCATTAAGGCCCTGCCAGCTTCGCCGCTCTAACACCTGCTTAATGATTCGGC; AGCTCAGGGGCACATAAGCCCCACCGAAGACGTAAGCCCTGTCTCGGGGCACTTTCAGAT; ATACTCGCCCGGCCACACGTGGGGATCAAATCCACTTTTTGCTGATGGACCAAAATTGCT; CCTTTTTGCCACAAAACTGGAAGCATCATTAATCTTTCCTGGCATCTTGGTCGTCACCAG; CTTGTTCACTTTACTTTCCAGCTTTGAAAGGTGTCCCCGGGGCCCTGTCCCTAGGAACCC; CTCTTTTCCAAATGGAAAAGTTACCAGTGCTCACGGCCT;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_018668
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018668.2, NP_061138.2
• RefSeq Size: 2509 bp
• RefSeq ORF: 1854 bp
• Locus ID: 26276
• UniProt ID: Q9H267
• Cytogenetics: 15q26.1
• Domains: Sec1
• Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).