HLA-DRA (NM_019111) Human Untagged Clone

SKU SC113252

HLA (untagged)-Human major histocompatibility complex, class II, DR alpha (HLA-DRA)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HLA-DRA
• Synonyms: HLA-DRA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_019111, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCATAAGTGGAGTCCCTGTGCTAGGATTTTTCATCATAGCTGTGCTGATGAGCGCTCAGGAATCAT; GGGCTATCAAAGAAGAACATGTGATCATCCAGGCCGAGTTCTATCTGAATCCTGACCAATCAGGCGAGTT; TATGTTTGACTTTGATGGTGATGAGATTTTCCATGTGGATATGGCAAAGAAGGAGACGGTCTGGCGGCTT; GAAGAATTTGGACGATTTGCCAGCTTTGAGGCTCAAGGTGCATTGGCCAACATAGCTGTGGACAAAGCCA; ACCTGGAAATCATGACAAAGCGCTCCAACTATACTCCGATCACCAATGTACCTCCAGAGGTAACTGTGCT; CACAAACAGCCCTGTGGAACTGAGAGAGCCCAACGTCCTCATCTGTTTCATAGACAAGTTCACCCCACCA; GTGGTCAATGTCACGTGGCTTCGAAATGGAAAACCTGTCACCACAGGAGTGTCAGAGACAGTCTTCCTGC; CCAGGGAAGACCACCTTTTCCGCAAGTTCCACTATCTCCCCTTCCTGCCCTCAACTGAGGACGTTTACGA; CTGCAGGGTGGAGCACTGGGGCTTGGATGAGCCTCTTCTCAAGCACTGGGAGTTTGATGCTCCAAGCCCT; CTCCCAGAGACTACAGAGAACGTGGTGTGTGCCCTGGGCCTGACTGTGGGTCTGGTGGGCATCATTATTG; GGACCATCTTCATCATCAAGGGATTGCGCAAAAGCAATGCAGCAGAACGCAGGGGGCCTCTGTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019111 unedited; GTTAGATTTGTATACGACTTACTATAGGCGGCCGCGACTTCGGCACGAGGCCTCGTGCCG; AATTCGGCACGAGGCCTCACTCCCGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGA; AAATGGCCATAAGTGGAGTCCCTGTGCTAGGATTTTTCATCATAGCTGTGCTGATGAGCG; CTCAGGAATCATGGGCTATCAAAGAAGAACATGTGATCATCCAGGCCGAGTTCTATCTGA; ATCCTGACCAATCAGGCGAGTTTATGTTTGACTTTGATGGTGATGAGATTTTCCATGTGG; ATATGGCAAAGAAGGAGACGGTCTGGCGGCTTGAAGAATTTGGACGATTTGCCAGCTTTG; AGGCTCAAGGTGCATTGGCCAACATAGCTGTGGACAAAGCCAACCTGGAAATCATGACAA; AGCGCTCCAACTATACTCCGATCACCAATGTACCTCCAGAGGTAACTGTGCTCACGAACA; GCCCTGTGGAACTGAGAGAGCCCAACGTCCTCATCTGTTTCATAGACAAGTTCACCCCAC; CAGTGGTCAATGTCACGTGGCTTCGAAATGGAAAACCTGTCACCACAGGAGTGTCAGAGA; CAGTCTTCCTGCCCAGGGAAGACCACCTTTTCCGCAAGTTCCACTATCTCCCCTTCCTGC; CCTCAACTGAGGACGTTTACGACTGCAGGNTGGAGCACTGGGGCTTGGATGAGCCTCTTC; TAAGCACTGGGAGTTTGATGCTCCAAGCCCTCTCCCAGAGACTACAGAGAACGTGGNTGT; GTGCCCTGGGCCTGACTGGGGGTCTGCTGGGCATCATTATTGGGACCCATCTCATCATCA; GGGGATTGCGCAAAAGCATGCAGCAGACGCAGGGGCCTCTGTAGGACCATGAGTGGATAG; GGTTTCTTAAAAAGAGATACTGGAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019111 unedited; CGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTCCACCCAAAATCTTCAAA; ATAGTTTATTGTGTTCAGAAAAATCACAGTTTGAGGAAACCTTAAACAATAAGTACACAA; GAGATGCCCCATGGGGCATTCCATAGCAGAGACAGACTCCTGTATGTTTTATTCCAGAGG; CATTGCATGGTGATAATAAAATGATAGGAAATAGAGGAAAATAGATACAGGAAAAGGCAA; TAGACAGGGAAGCCAGCTAGATGTTAGAGTATGGAGCAATCGAGGAGGCATAACCACTCT; TGGGGTAGCTATAGGGCTGGAAAATGCTGAAGATGACTGCTTTCACTGAGGTCAAGGATT; GTAATATTGCCAGCTTTGTAAAGCCATTAAAGCAGAAGTTTCTTCAGTGATCTTCTCTCT; AAGAAACACCATCACCTCCATGTGCCTTACAGAGGCCCCCTGCGTTCTGCTGCATTGCTT; TTGCGCAATCCCTTGATGATGAAGATGGTCCCAATAATGATGCCCACCAGACCCACAGTC; AGGCCCAGGGCACACACCACGTTCTCTGTAGTCTCTGGGAGAGGGCTTGGAGCATCAAAC; TCCCAGTGCTTGAGAAGAGGCTCATCCAAGCCCCAGTGCTCCACCCTGCAGTCGTAAACG; TCCTCAGTTGAGGGCAGGAAGGGGAGATAGTGGAACTTGCGGAAAAGGTGGTCTTCCCTG; GGCAGGAAGACTGTCTCTGACACTCCTGTGGTGACAGGTTTTCCATTTCGAAGCCACGTG; ACATTGACCACTGGTGGGNGTGAACCTGTCTATGAAACAGATGAGGACGTTGGGCTCTCT; CAGTCCACAGGGNCTGTCGTGAGACAGNTACCTCTGGAGTACATTGGTGGATCGAGTATA; TTGGAGCGCTTGTCATGATTCAGTTGGCTTGTCCCACTTATGTGCCAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_019111
• Insert Size: 1340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019111.3, NP_061984.2
• RefSeq Size: 1267 bp
• RefSeq ORF: 765 bp
• Locus ID: 3122
• UniProt ID: P01903
• Cytogenetics: 6p21.32
• Domains: ig, IGc1, MHC_II_alpha
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Summary: HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq, Aug 2020]