DLL4 (NM_019074) Human Untagged Clone

CAT#: SC113239

DLL4 (untagged)-Human delta-like 4 (Drosophila) (DLL4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DLL4
• Synonyms: AOS6; delta4; hdelta2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113239 sequence for NM_019074 edited (data generated by NextGen Sequencing)
  ATGGCGGCAGCGTCCCGGAGCGCCTCTGGCTGGGCGCTACTGCTGCTGGTGGCACTTTGG
  CAGCAGCGCGCGGCCGGCTCCGGCGTCTTCCAGCTGCAGCTGCAGGAGTTCATCAACGAG
  CGCGGCGTACTGGCCAGTGGGCGGCCTTGCGAGCCCGGCTGCCGGACTTTCTTCCGCGTC
  TGCCTTNAGCACTTCCAGGCGGTCGTCTCGCCCGGACCCTGCACCTTCGGGACCGTCTCC
  ACGCCGGTATTGGGCACCAACTCCTTCGCTGTCCGGGACGACAGTAGCGGCGGGGGGCGC
  AACCCTCTCCAACTGCCCTTCAATTTCACCTGGCCGGGTACCTTCTCGCTCATCATCGAA
  GCTTGGCACGCGCCAGGAGACGACCTGCGGCCAGAGGCCTTGCCACCAGATGCACTCATC
  AGCAAGATCGCCATCCAGGGCTCCCTAGCTGTGGGTCAGAACTGGTTATTGGATGAGCAA
  ACCAGCACCCTCACAAGGCTGCGCTACTCTTACCGGGTCATCTGCAGTGACAACTACTAT
  GGAGACAACTGCTCCCGCCTGTGCAAGAAGCGCAATGACCACTTCGGCCACTATGTGTGC
  CAGCCAGATGGCAACTTGTCCTGCCTGCCCGGTTGGACTGGGGAATATTGCCAACAGCCT
  ATCTGTCTTTCGGGCTGTCATGAACAGAATGGCTACTGCAGCAAGCCAGCAGAGTGCCTC
  TGCCGCCCAGGCTGGCAGGGCCGGCTGTGTAACGAATGCATCCCCCACAATGGCTGTCGC
  CACGGCACCTGCAGCACTCCCTGGCAATGTACTTGTGATGAGGGCTGGGGAGGCCTGTTT
  TGTGACCAAGATCTCAACTACTGCACCCACCACTCCCCATGCAAGAATGGGGCAACGTGC
  TCCAACAGTGGGCAGCGAAGCTACACCTGCACCTGTCGCCCAGGCTACACTGGTGTGGAC
  TGTGAGCTGGAGCTCAGCGAGTGTGACAGCAACCCCTGTCGCAATGGAGGCAGCTGTAAG
  GACCAGGAGGATGGCTACCACTGCCTGTGTCCTCCGGGCTACTATGGCCTGCATTGTGAA
  CACAGCACCTTGAGCTGCGCCGACTCCCCCTGCTTCAATGGGGGCTCCTGCCGGGAGCGC
  AACCAGGGGGCCAACTATGCTTGTGAATGTCCCCCCAACTTCACCGGCTCCAACTGCGAG
  AAGAAAGTGGACAGGTGCACCAGCAACCCCTGTGCCAACGGGGGACAGTGCCTGAACCGA
  GGTCCAAGCCGCATGTGCCGCTGCCGTCCTGGATTCACGGGCACCTACTGTGAACTCCAC
  GTCAGCGACTGTGCCCGTAACCCTTGCGCCCACGGTGGCACTTGCCATGACCTGGAGAAT
  GGGCTCATGTGCACCTGCCCTGCCGGCTTCTCTGGCCGACGCTGTGAGGTGCGGACATCC
  ATCGATGCCTGTGCCTCGAGTCCCTGCTTCAACAGGGCCACCTGCTACACCGACCTCTCC
  ACAGACACCTTTGTGTGCAACTGCCCTTATGGCTTTGTGGGCAGCCGCTGCGAGTTCCCC
  GTGGGCTTGCCGCCCAGCTTCCCCTGGGTGGCCGTCTCGCTGGGTGTGGGGCTGGCAGTG
  CTGCTGGTACTGCTGGGCATGGTGGCAGTGGCTGTGCGGCAGCTGCGGCTTCGACGGCCG
  GACGACGGCAGCAGGGAAGCCATGAACAACTTGTCGGACTTCCAGAAGGACAACCTGATT
  CCTGCCGCCCAGCTTAAAAACACAAACCAGAAGAAGGAGCTGGAAGTGGACTGTGGCCTG
  GACAAGTCCAACTGTGGCAAACAGCAAAACCACACATTGGACTATAATCTGGCCCCAGGG
  CCCCTGGGGCGGGGGACCATGCCAGGAAAGTTTCCCCACAGTGACAAGAGCTTAGGAGAG
  AAGGCGCCACTGCGGTTACACAGTGAAAAGCCAGAGTGTCGGATATCAGCGATATGCTCC
  CCCAGGGACTCCATGTACCAGTCTGTGTGTTTGATATCAGAGGAGAGGAATGAATGTGTC
  ATTGCCACGGAGGTATAA
  
  Clone variation with respect to NM_019074.3
  187 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019074 unedited
  CCCCCGCCCTGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG
  AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
  TTCGGCACGAGGCTGCGCGCAGGCCGGGAACACGAGGCCAAGAGCCGCAGCCCCAGCCGC
  CTTGGTGCAGCGTACACCGGCACTAGCCCGCTTGCAGCCCCAGGATTAGACAGAAGACGC
  GTCCTCGGCGCGGTCGCCGCCCAGCCGTAGTCACCTGGATTACCTACAGCGGCAGCTGCA
  GCGGAGCCAGCGAGAAGGCCAAAGGGGAGCAGCGTCCCGAGAGGAGCGCCTCTTTTCAGG
  GACCCCGCCGGCTGGCGGACGCGCGGGAAAGCGGCGTCGCGAACAGAGCCAGATTGAGGG
  CCCGCGGGTGGAGAGAGCGACGCCCGAGGGGATGGCGGCAGCGTCCCGGAGCGCCTCTGG
  CTGGGCGCTACTGCTGCTGGTGGCACTTTGGCAGCAGCGCGCGGCCGGCTCCGGCGTCTT
  CCAGCTGCAGCTGCAGGAGTTCATCAACGAGCGCGGCGTACTGGCCAGTGGGCGGCCTTG
  CGAGCCCGGCTGCCGGACTTTCTTCCGCGTCTGCCTTAAGCACTTCCAGGCGGTCGTCTC
  GCCCGGACCCTGCACCTTCGGGACCGTCTCCACGCCGGTATTGGGCACCAACTCCTTCGC
  TGTCCGGGACGACAGTAGCGGCGGGGGGCGNCACCCTCTNCACTGCCCTTCATNTCACCT
  GGCCGGGTACCTTCTCGCTCATCATCGAAGCTGGCCACGCGCCAGGAGACGACCTGGCGG
  CAGAGGCCTTGNCACCAGATGCACTCATCAGCAAGATCGCCATCCAGGGCTCCCTAGCTG
  TGGGTCAGAACTGGGTATGGGNATGAGCAACAGCACCTNACAGGCTGCGCTACTCTTACC
  GGTCATCTGCATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019074 unedited
  GTTTATCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCTG
  TACACATCTGTTTCATATTATAAATACACATTATATACAAAATAATGTTATAAAATGTAG
  AAAGGTCAGTGCTTCTGATTCTTAAATTATTGAAATACTAGACTCCAAAATAAACTACAA
  AAAAAAAAAAAAAAACAAAATAAAAACTCATATTATTGATTTCTGAAAACCAGCTTTGAG
  GGTGGTCTGGGGCAGTAGTGGACCAAAGCCCAGGCTCGAGGTTGTGGAAATGGGCTTGAG
  GGAGGAAAGGGCCCAGGCGGCCGCAGCACCTGATTTCCCCCTCAGTGGAATTTCCAACAC
  ACTGCCCTGAGTGCCCCAGTTAGGCCTGGCACCCTTGGCTGGTGGGAGGGGCAGGCCCTC
  TGCCTGTCTGCTTACAGAAGCCCAGGGCCAAAGGGTGGTGGAAGCCGGGTAAACGGACAT
  TCTTGCCCAAAGGGCCTAGACCAAAGAGCCAGGCCTGCATACAGTAATTGGAGGCATGGG
  GCANAAAGCACTCCCCTCCCTNGAGCACTCACCTCACCTCCAGGGGAAACCCCAGCAAGA
  GACCCACCCCAGTGGTTTTTCAACCCACAAGAGGCCCCTGCCCTCATTGGCCCCCCTCTC
  TCCTTTCTGATTTGGGCCCCAACTGTCGCCGTGGAGACCCGGGCCAAAGCACCTTTTGGA
  ACACCGAAGCCCATTGGAGGCCACAAAAACTTCCGGGCCCCATGGGGCTACTGGCACCTC
  TCTTCGGGAAAAAGGGCCGGAACCCCGGAAGGAGCCCCCTGGAGGCCCCTTATGGGAGGG
  GAAGGGCCCAATGGCTTCCTCTCTCCCTTTTGGTCCCCCGGGGGGGGGGCACTTGCCATT
  TTGATGCCCAAAGAACCCCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_019074
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019074.2, NP_061947.1
• RefSeq Size: 3383 bp
• RefSeq ORF: 2058 bp
• Locus ID: 54567
• UniProt ID: Q9NR61
• Cytogenetics: 15q15.1
• Domains: DSL, EGF
• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: Notch signaling pathway
• Gene Summary: This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq, Jul 2008]