CysLT2 (CYSLTR2) (NM_020377) Human Untagged Clone

SKU SC113111

CYSLTR2 (untagged)-Human cysteinyl leukotriene receptor 2 (CYSLTR2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CysLT2
• Synonyms: CYSLT2; CYSLT2R; GPCR21; HG57; hGPCR21; HPN321; KPG_011; PSEC0146
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC113111 representing NM_020377.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGGAGAGAAAATTTATGTCCTTGCAACCATCCATCTCCGTATCAGAAATGGAACCAAATGGCACCTTC; AGCAATAACAACAGCAGGAACTGCACAATTGAAAACTTCAAGAGAGAATTTTTCCCAATTGTATATCTG; ATAATATTTTTCTGGGGAGTCTTGGGAAATGGGTTGTCCATATATGTTTTCCTGCAGCCTTATAAGAAG; TCCACATCTGTGAACGTTTTCATGCTAAATCTGGCCATTTCAGATCTCCTGTTCATAAGCACGCTTCCC; TTCAGGGCTGACTATTATCTTAGAGGCTCCAATTGGATATTTGGAGACCTGGCCTGCAGGATTATGTCT; TATTCCTTGTATGTCAACATGTACAGCAGTATTTATTTCCTGACCGTGCTGAGTGTTGTGCGTTTCCTG; GCAATGGTTCACCCCTTTCGGCTTCTGCATGTCACCAGCATCAGGAGTGCCTGGATCCTCTGTGGGATC; ATATGGATCCTTATCATGGCTTCCTCAATAATGCTCCTGGACAGTGGCTCTGAGCAGAACGGCAGTGTC; ACATCATGCTTAGAGCTGAATCTCTATAAAATTGCTAAGCTGCAGACCATGAACTATATTGCCTTGGTG; GTGGGCTGCCTGCTGCCATTTTTCACACTCAGCATCTGTTATCTGCTGATCATTCGGGTTCTGTTAAAA; GTGGAGGTCCCAGAATCGGGGCTGCGGGTTTCTCACAGGAAGGCACTGACCACCATCATCATCACCTTG; ATCATCTTCTTCTTGTGTTTCCTGCCCTATCACACACTGAGGACCGTCCACTTGACGACATGGAAAGTG; GGTTTATGCAAAGACAGACTGCATAAAGCTTTGGTTATCACACTGGCCTTGGCAGCAGCCAATGCCTGC; TTCAATCCTCTGCTCTATTACTTTGCTGGGGAGAATTTTAAGGACAGACTAAAGTCTGCACTCAGAAAA; GGCCATCCACAGAAGGCAAAGACAAAGTGTGTTTTCCCTGTTAGTGTGTGGTTGAGAAAGGAAACAAGA; GTATAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020377 unedited; TCATATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGANAAAGCCTTC; TGTCATCTTCCTGGGGATCACCTCTTCAGGTGTACAGAGACAGCATAGNGGAAAACTAGG; TTCCAAGATGGCTGAATAGGAAGAGCTCCAATCTGCAGATCCCAGTGTGAGCAACGTGGA; AGACGGGTGATTTCTGCATTTCCAACTGAGCATGGAGAGAAAATTTATGTCCTTGCAACC; ATCCATCTCCGTATCAGAAATGGAACCAAATGGCACCTTCAGCAATAACAACAGCAGGAA; CTGCACAATTGAAAACTTCAAGAGAGAATTTTTCCCAATTGTATATCTGATAATATTTTT; CTGGGGAGTCTTGGGAAATGGGTTGTCCATATATGTTTTCCTGCAGCCTTATAAGAAGTC; CACATCTGTGAACGTTTTCATGCTAAATCTGGCCATTTCAGATCTCCTGTTCATAAGCAC; GCTTCCCTTCAGGGCTGACTATTATCTTAGAGGCTCCAATTGGATATTTGGAGACCTGGC; CTGCAGGATTATGTCTTATTCCTTGTATGTCAACATGTACAGCAGTATTTATTTCCTGAC; CGTGCTGAGTGTTGTGCGTTTCCTGGCAATGGTTCACCCCTTTCGGCTTCTGCATGTCAC; CAGCATCAGGAGTGCCTGGATCCTCTGTGGGATCATATGGATCCTTATCATGGCTTCCTC; AATAATGCTCCTGGACAGTGGCTCTGAGCAGAACGGCAGTGTCACATCATGCTTAGAGCT; GAATCTCTATAAAATTGCTAAGCTGCAGACCATGAACTATATTGCCTTGGTGGTGGGCTG; CCTGCTGCCATTTTTCACACTCAGCATCTGTTATCTGCTGNATCATTCGGNTCTGTTAAA; AGTGGAGTCCCCAGAATCGNGCTGCGGGNTTCTCACAGAAGGCACTGACCACCTCTCATC; ACCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_020377
• Insert Size: 1041 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020377.2
• RefSeq Size: 2548 bp
• RefSeq ORF: 1041 bp
• Locus ID: 57105
• UniProt ID: Q9NS75
• Cytogenetics: 13q14.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• MW: 39.6 kDa
• Summary: The cysteinyl leukotrienes LTC4, LTD4, and LTE4 are important mediators of human bronchial asthma. Pharmacologic studies have determined that cysteinyl leukotrienes activate at least 2 receptors, the protein encoded by this gene and CYSLTR1. This encoded receptor is a member of the superfamily of G protein-coupled receptors. It seems to play a major role in endocrine and cardiovascular systems. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (VI) differs in the 5' UTR compared to variant I. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.