G0 Protein alpha (GNAO1) (NM_020988) Human Untagged Clone

CAT#: SC113061

GNAO1 (untagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: G0 Protein alpha
• Synonyms: DEE17; EIEE17; G-ALPHA-o; GNAO; HLA-DQB1; NEDIM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113061 sequence for NM_020988 edited (data generated by NextGen Sequencing)
  ATGGGATGTACTCTGAGCGCAGAGGAGAGAGCCGCCCTCGAGCGGAGCAAGGCGATTGAG
  AAAAACCTCAAAGAGGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTCGGG
  GCTGGAGAATCAGGAAAAAGCACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGC
  TTCTCCGGAGAAGACGTGAAACAGTACAAGCCTGTTGTCTACAGCAACACTATCCAGTCC
  CTGGCAGCCATCGTCCGGGCCATGGACACTTTGGGCATCGAATATGGTGATAAGGAGAGA
  AAGGCTGACGCCAAGATGGTGTGTGATGTGGTGAGTCGGATGGAAGACACCGAGCCCTTC
  TCTGCAGAGCTGCTTTCTGCCATGATGCGGCTCTGGGGCGACTCAGGAATCCAAGAGTGC
  TTCAACCGGTCCCGGGAGTATCAGCTCAACGACTCTGCCAAATACTACCTGGACAGCCTG
  GATCGGATTGGGGCCGCCGACTACCAGCCCACTGAGCAGGACATCCTCCGAACCAGGGTC
  AAAACCACTGGCATCGTAGAAACCCACTTCACATTCAAGAACCTCCACTTCAGGCTGTTT
  GACGTCGGAGGCCAGCGATCTGAACGCAAGAAGTGGATCCATTGCTTCGAGGACGTCACG
  GCCATCATTTTCTGTGTCGCGCTCAGCGGCTATGACCAGGTGCTCCACGAAGACGAAACC
  ACGAACCGCATGCACGAGTCTCTCATGCTCTTCGACTCCATCTGTAACAACAAGTTCTTC
  ATCGATACCTCCATCATTCTCTTCCTCAACAAGAAAGATCTCTTTGGCGAGAAGATCAAG
  AAGTCACCTTTGACCATCTGCTTTCCTGAATACACAGGCCCCAATACCTATGAAGACGCA
  GCCGCCTACATCCAAGCACAATTTGAAAGCAAAAACCGCTCACCCAACAAAGAAATATAT
  TGTCACATGACTTGTGCCACAGACACGAATAACATCCAGGTGGTGTTCGACGCCGTCACC
  GACATCATCATTGCCAACAACCTCCGGGGCTGCGGCTTGTACTGA
  
  Clone variation with respect to NM_020988.2
  513 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020988 unedited
  TCANGATATTTGTATACGACTTACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGGCGG
  GCTGTGGCAGGGAAGGGGCCACCATGGGATGTACTCTGAGCGCAGAGGAGAGAGCCGCCC
  TCGAGCGGAGCAAGGCGATTGAGAAAAACCTCAAAGAGGATGGCATCAGCGCCGCCAAAG
  ACGTGAAATTACTCCTGCTCGGGGCTGGAGAATCAGGAAAAAGCACCATTGTGAAGCAGA
  TGAAGATCATCCATGAAGATGGCTTCTCCGGAGAAGACGTGAAACAGTACAAGCCTGTTG
  TCTACAGCAACACTATCCAGTCCCTGGCAGCCATCGTCCGGGCCATGGACACTTTGGGCA
  TCGAATATGGTGATAAGGAGAGAAAGGCTGACGCCAAGATGGTGTGTGATGTGGCGAGTC
  GGATGGAAGACACCGAGCCCTTCTCTGCAGAGCTGCTTTCTGCCATGATGCGGCTCTGGG
  GCGACTCAGGAATCCAAGAGTGCTTCAACCGGTCCCGGGAGTATCAGCTCAACGACTCTG
  CCAAATACTACCTGNACAGCCTGGATCGGATTGGGGCCGCCGACTACCAGCCCACTGAGC
  AGGACATCCTCCGAACCAGGGTCAAAACCACTGGCATCGTAGAAACCCACTTCACATTCA
  AGAACCTTCACTTCAGGCTGTTTGACGTCGGAGGCCAGCGATCTGAACGCAAGAAGGGAT
  CCATTGCTTCGAGGACGTCACGGCCATCATTTCTGTGTCGCGCTCACCGGCTATGACCCA
  GTGCTCCACGAAGACCAAACCCCAACCCGCATGCCGAGTCTCTCATGCTCTTCGACTCCT
  CTGAACAACAAGTCCTCATCGATACCTCATCATTCTCTTTCTCAACAGAAAGATCCCTCT
  GCCGAGAGAACCAGAAGTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020988 unedited
  ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTGTGTTTTGTCTTTTT
  GCCTCCTCTTTATTTGAAATGGCTGTGACTGGTCTTCTTCAGCAAAGATTAAGTCTTAAA
  AGTGAAACCGTGAAGAGGTTTAGGGTTTGGGTATTAGTCAAAGTAGAAATGACATAGGAT
  TGGTCATGAAAACGGCGGCTTTAATGTAAACACTACATTCCATCTTTTTAAATTTTTTTG
  TTTTTTTTTTCCATCTTGTGGTATTTAAAAATTTTTTTTTGTTCTTTTTGTCTGCAGCAC
  AAACATCCCCACATGAGAGAGCGGACACAGGGCACCGAAACAAAGGCAGGGGTGGGGAGG
  GGCGCACACACGAAGGGAGGTGTCAGCCGGGACCGGAAATCCAACACGGCAAAGGAAAAA
  AAACACAACCCGTTTCCCAAAGACTAGGAAAACTGATCTGTGGGGTGGGGGGCTGGTAAT
  ACATAGTACTCGGCGGGACAAAAGTAAAAACAAAAACTGAAAAAAAAAAAAAAGGAACCA
  AAAAACCCCTACAAAGGCTATCCTTANAAAGGGGGTCGGTGGGCANATTGTTTTCTATGG
  GGCTGGATTTCCTTATACGATGGGTGTTCTTTTTGGCAGCTACTGGCCAGTGAGAGCTGT
  GCTTTTTCTATACCAACCTGGAGTGANCGTGTGGCGGGATGGCTTANGGCCCCCACAGTG
  CCTCTGTGGTGTCTGGGCCGCCCGCAGGCACCAGGGGTTGCAGTGGGCCTGGGGCCTTCT
  GGAAAAGCCCGCCCGAGCCCACTCCTTCGACCTTGCACCCGACCGGAACCCCAGCGGCAG
  ATGGGCCCTGCCCCCACAGAACTTCAGGGCTTCCCCCAAAGGGGAACGGGCCAGCCCCGG
  CCAATAGGTTTTTTTTTTCAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020988
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020988.1, NP_066268.1
• RefSeq Size: 2215 bp
• RefSeq ORF: 1065 bp
• Locus ID: 2775
• UniProt ID: P09471
• Cytogenetics: 16q13
• Domains: G-alpha
• Protein Families: Druggable Genome
• Protein Pathways: Long-term depression, Melanogenesis
• Gene Summary: The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes isoform a.