AMY2B (NM_020978) Human Untagged Clone

SKU SC113058

AMY2B (untagged)-Human amylase, alpha 2B (pancreatic) (AMY2B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: AMY2B
• Synonyms: AMY2; AMY3; HXA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113058 sequence for NM_020978 edited (data generated by NextGen Sequencing); ATGAAGTTCTTTCTGTTGCTTTTCACCATTGGGTTCTGCTGGGCTCAGTATTCCCCAAAT; ACACAACAAGGACGGACATCTATTGTTCATCTGTTTGAATGGCGATGGGTTGATATTGCT; CTTGAATGTGAGCGATATTTAGCTCCCAAGGGATTTGGAGGGGTTCAGGTCTCTCCACCA; AATGAAAATGTTGCAATTCACAACCCTTTCAGACCTTGGTGGGAAAGATACCAACCAGTT; AGCTATAAATTATGCACAAGATCTGGAAATGAAGATGAATTTAGAAACATGGTGACTAGA; TGTAACAATGTTGGGGTTCGTATTTATGTGGATGCTGTAATTAATCATATGTCTGGTAAT; GCTGTGAGTGCAGGAACAAGCAGTACCTGTGGAAGTTACTTCAACCCTGGAAGTAGGGAC; TTTCCAGCAGTCCCATATTCTGGATGGGATTTTAATGATGGTAAATGTAAAACTGGAAGT; GGAGATATCGAGAACTACAATGATGCTACTCAGGTCAGAGATTGTCGTCTGGTTGGTCTT; CTTGATCTTGCACTGGAGAAAGATTATGTGCGTTCCAAGATTGCCGAATATATGAATCAT; CTCATTGACATTGGTGTTGCAGGGTTCAGACTTGATGCTTCCAAGCACATGTGGCCTGGA; GACATAAAGGCAATTTTGGACAAACTGCATAATCTAAACAGTAACTGGTTCCCTGCAGGA; AGTAAACCTTTCATTTACCAGGAGGTAATTGATCTGGGTGGTGAGCCAATTAAAAGCAGT; GACTACTTTGGAAATGGCCGGGTGACAGAATTCAAGTATGGTGCAAAACTCGGCACAGTT; ATTCGCAAGTGGAATGGAGAGAAGATGTCTTACCTAAAGAACTGGGGAGAAGGTTGGGGT; TTCATGCCTTCTGACAGAGCACTTGTCTTTGTGGATAACCATGACAATCAACGAGGACAT; GGGGCTGGAGGAGCCTCTATTCTTACCTTCTGGGATGCTAGGCTGTATAAAATGGCAGTT; GGATTTATGCTTGCTCATCCTTATGGTTTTACACGAGTAATGTCAAGCTACCGTTGGCCA; AGACAGTTTCAAAATGGAAACGATGTTAATGATTGGGTTGGGCCACCAAATAATAATGGA; GTAATTAAAGAAGTTACTATTAATCCAGACACTACTTGTGGCAATGACTGGGTCTGTGAA; CATCGATGGCGCCAAATAAGGAACATGGTTAATTTCCGCAATGTAGTGGATGGCCAGCCT; TTTACAAACTGGTATGATAATGGGAGCAACCAAGTGGCTTTTGGGAGAGGAAACAGAGGA; TTCATTGTTTTCAACAATGATGACTGGACATTTTCTTTAACTTTGCAAACTGGTCTTCCT; GCTGGCACATACTGTGATGTCATTTCTGGAGATAAAATTAATGGCAATTGCACAGGCATT; AAAATCTACGTTTCTGACGATGGCAAAGCTCATTTTTCTATTAGTAACTCTGCTGAGGAT; CCATTTATTGCAATTCATGCTGAATCTAAATTATAA; ; Clone variation with respect to NM_020978.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020978 unedited; GTTCAGATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACTCAACTA; CTATTAGCTGGCGAGTCAACTCACGTTGTTGGAATGACTAGGGACAACTAGACTTCAAGT; GATCTTTCATCCCAGGAATATAAATAGTTTCTGGAAAGGACACTGACAACTTCAAAGCAA; AATGAAGTTCTTTCTGTTGCTTTTCACCATTGGGTTCTGCTGGGCTCAGTATTCCCCAAA; TACACAACAAGGACGGACATCTATTGTTCATCTGTTTGAATGGCGATGGGTTGATATTGC; TCTTGAATGTGAGCGATATTTAGCTCCCAAGGGATTTGGAGGGGTTCAGGTCTCTCCACC; AAATGAAAATGTTGCAATTCACAACCCTTTCAGACCTTGGTGGGAAAGATACCAACCAGT; TAGCTATAAATTATGCACAAGATCTGGAAATGAAGATGAATTTAGAAACATGGTGACTAG; ATGTAACAATGTTGGGGTTCGTATTTATGTGGATGCTGTAATTAATCATATGTCTGGTAA; TGCTGTGAGTGCAGGAACAAGCAGTACCTGTGGAAGTTACTTCAACCCTGGAAGTAGGGA; CTTTCCAGCAGTCCCATATTCTGGATGGGATTNTAATGATGGTAAATGTAAAACTGGAAG; TGGAGATATCGAGAACTACAATGATGCTACTCANGTCAGAGATTGTCGTCTGGTTGGTCT; TCTTGATCTTGCACTGGAGAAAGATATGTGCCGTCCAGATTGCCGATATATGAATCATCT; CATTGACATTGNTGTTGCAGGGNTCAGACTTGATGCTTCCAACACATGTGGCCTGNAGAC; ACTAAGGCCATTTGGNACAAACTGCATAATCTAACAGTNACTGTNTCCNTGCCAGAAGTA; ACCCTTCATTTACCAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020978 unedited; ATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTTTTGAGGATA; TGCATTTAATTTTAAATTTTATAATTTAGATTCAGCATGAATTGCAATAAATGGATCCTC; AGCAGAGTTACTAATAGAAAAATGAGCTTTGCCATCGTCAGAAACGTAGATTTTAATGCC; TGTGCAATTGCCATTAATTTTATCTCCAGAAATGACATCACAGTATGTGCCAGCAGGAAG; ACCAGTTTGCAAAGTTAAAGAAAATGTCCAGTCATCATTGTTGAAAACAATGAATCCTCT; GTTTCCTCTCCCAAAAGCCACTTGGTTGCTCCCATTATCATACCAGTTTGTAAAAGGCTG; GCCATCCACTACATTGCGGAAATTAACCATGTTCCTTATTTGGCGCCATCGATGTTCACA; GACCCAGTCATTGCCACAAGTAGTGTCTGGATTAATAGTAACTTCTTTAATTACTCCATT; ATTATTTGGTGGCCCAACCCAATCATTAACATCGTTTCCATTTTGAAACTGTCTTGGCCA; ACGGTAGCTTGACATTACTCGTGTAAAACCATAAGGATGAGCAAGCATAAATCCAACTGC; CATTTTATACAGCCTAGCATCCCAGAAGGTAAGAATAGAGGCTCCTCCAGCCCCATGTCC; TCGTTGATTGTCATGGTTATCCACAAAGACAAGTGCTCTGTCAGAAGGCATGAAACCCCA; ACCTTCTCCCCAGTTCTTTAGGTAAGACATCTCTCTCCATTCCACTTGCGAATAACTGTG; CCGAGTTNTGACCATACTTGAATCTGTCACCGGNCATTCCAAGTAGTACTGCTTTAATTG; CTCACACCCAGATCATACCTCTGTAATGAAGGTTACTTCTGCAGGNACAGTCTGTTAGAT; ATGCAGTGTCCAAATGCCTTTGTCTCAGCCCAGTGCTGGAGCTCAGCTGAACTGCACACA; TGTATN;
• Restriction Sites: NotI-NotI
• ACCN: NM_020978
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020978.3, NP_066188.1
• RefSeq Size: 2215 bp
• RefSeq ORF: 1536 bp
• Locus ID: 280
• UniProt ID: P19961
• Cytogenetics: 1p21.1
• Domains: Aamy, Aamy_C, alpha-amylase
• Protein Families: Secreted Protein
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism
• Summary: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jun 2013]