NMT1 (NM_021079) Human Untagged Clone

CAT#: SC113026

NMT1 (untagged)-Human N-myristoyltransferase 1 (NMT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NMT1
• Synonyms: NMT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113026 sequence for NM_021079 edited (data generated by NextGen Sequencing)
  ATGGCGGACGAGAGTGAGACAGCAGTGAAGCCGCCGGCACCTCCGCTGCCGCAGATGATG
  GAAGGGAACGGGAACGGCCATGAGCACTGCAGCGATTGCGAGAATGAGGAGGACAACAGC
  TACAACCGGGGTGGTTTGAGTCCAGCCAATGACACTGGAGCCAAAAAGAAGAAAAAGAAA
  CAAAAAAAGAAGAAAGAAAAAGGCAGTGAGACAGATTCAGCCCAGGATCAGCCTGTGAAG
  ATGAACTCTTTGCCAGCAGAGAGGATCCAGGAAATACAGAAGGCCATTGAGCTGTTCTCA
  GTGGGTCAGGGACCTGCCAAAACCATGGAGGAGGCTAGCAAGCGAAGCTACCAGTTCTGG
  GATACGCAGCCCGTCCCCAAGCTGGGCGAAGTGGTGAACACCCATGGCCCCGTGGAGCCT
  GACAAGGACAATATCCGCCAGGAGCCCTACACCCTGCCCCAGGGCTTCACCTGGGATGCT
  TTGGACCTGGGCGATCGTGGTGTGCTAAAAGAACTGTACACCCTCCTGAATGAGAACTAT
  GTGGAAGATGATGACAACATGTTCCGATTTGATTATTCCCCGGAGTTTCTTTTGTGGGCT
  CTCCGGCCACCCGGCTGGCTCCCCCAGTGGCACTGTGGGGTTCGAGTGGTCTCAAGTCGG
  AAATTGGTTGGGTTCATTAGCGCCATCCCAGCAAACATCCATATCTATGACACAGAGAAG
  AAGATGGTAGAGATCAACTTCCTGTGTGTCCACAAGAAGCTGCGTTCCAAGAGGGTTGCT
  CCAGTTCTGATCCGAGAGATCACCAGGCGGGTTCACCTGGAGGGCATCTTCCAAGCAGTT
  TACACTGCCGGGGTGGTACTACCAAAGCCCGTTGGCACCTGCAGGTATTGGCATCGGTCC
  CTAAACCCACGGAAGCTGATTGAAGTGAAGTTCTCCCACCTGAGCAGAAATATGACCATG
  CAGCGCACCATGAAGCTCTACCGACTGCCAGAGACTCCCAAGACAGCTGGGCTGCGACCA
  ATGGAAACAAAGGACATTCCAGTAGTGCACCAGCTCCTCACCAGGTACTTGAAGCAATTT
  CACCTTACGCCCGTCATGAGCCAGGAGGAGGTGGAGCACTGGTTCTACCCCCAGGAGAAT
  ATCATCGACACTTTCGTGGTGGAGAACGCAAACGGAGAGGTGACAGATTTCCTGAGCTTT
  TATACGCTGCCCTCCACCATCATGAACCATCCAACCCACAAGAGTCTCAAAGCTGCTTAT
  TCTTTCTACAACGTTCACACCCAGACCCCTCTTCTAGACCTCATGAGCGACGCCCTTGTC
  CTCGCCAAAATGAAAGGGTTTGATGTGTTCAATGCACTGGATCTCATGGAGAACAAAACC
  TTCCTGGAGAAGCTCAAGTTTGGCATAGGGGACGGCAACCTGCAGTATTACCTTTACAAT
  TGGAAATGCCCCAGCATGGGGGCAGAGAAGGTTGGACTGGTGCTACAATAA
  
  Clone variation with respect to NM_021079.3
  487 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021079 unedited
  NAAAGGTTCAAATTTAGTATACGACTCATTATAGGCGGCCGCGCCTCGTGCCGAATTCGG
  CACGAGGGAGCCCTGCTCTCGCACTCAAGAGGCGGACGAGAGTGAGACAGCAGTGAAGCC
  GCCGGCACCTCCGCTGCCGCAGATGATGGAAGGGAACGGGAACGGCCATGAGCACTGCAG
  CGATTGCGAGAATGAGGAGGACAACAGCTACAACCGGGGTGGTTTGAGTCCAGCCAATGA
  CACTGGAGCCAAAAAGAAGAAAAAGAAACAAAAAAAGAAGAAAGAAAAAGGCAGTGAGAC
  AGATTCAGCCCAGGATCAGCCTGTGAAGATGAACTCTTTGCCAGCAGAGAGGATCCAGGA
  AATACAGAAGGCCATTGAGCTGTTCTCAGTGGGTCAGGGACCTGCCAAAACCATGGAGGA
  GGCTAGCAAGCGAAGCTACCAGTTCTGGGATACGCAGCCCGTCCCCAAGCTGGGCGAAGT
  GGTGAACACCCATGGCCCCGTGGAGCCTGACAAGGACAATATCCGCCAGGAGCCCTACAC
  CCTGCCCCAGGGCTTCACCTGNGATGCTTTGGACCTGNGCGATCGTGGTGTGCTAAAAGA
  ACTGTACACCCTCCTGAATGAGAACTATGTGGAAGATGATGACAACATGTTCCGATTTGA
  TTATTCCCCGGAGTTTCTTTTGTGGGCTCTCCGGCCACCCGGCTGGCTCCCCCAGGGACT
  GTGTGGGGGTCGAGTGGTCTCAAGTCGGAAATTGTNTGGGNTCATTAGCGCCATCCCAGC
  AAACATCCATATCTATGACACAGAGAAGAAGAGGTNGAGATCAACTTCTGTGTGTNCACA
  AGAGCTGCNGTCCAGAAGGGTGCTCCAGTTCTGATCCGAGAGATCACCAGCGGGTCACCT
  GGAAGGCATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021079 unedited
  GGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTAATATATATATATACTTTTAT
  TATTCACCCGTTAACTCCATTATATGCCAATCACGAGCTAGTTTTCAGCAGTTACATTCC
  CTTGATCACGGCTGCATGAGGATGTAATTAACTGGAAAACACGGAGACCAGAGACACGAC
  CGGAGGTGACGTCACGCAGCCCACGCCATCGCAATACAATTGTCAAGTTCGCTGGCGGTT
  TGGTAAAGCCGGTTCAGTTCTGCTCCCTTTGCCAGGGATTCTCACGTGTGTGAAAATTGG
  ACCAACAGTGGTGGGGTTCCATTTCCTGGTTCGAATTTTCAGTGGCTTTATCCAGAATCG
  CACTGGTGACTGGTTATTGTAGCACCAGTCCAACCTTCTCTGCCCCCATGCTGGGGCATT
  TCCAATTGTAAAGGTAATACTGCAGGTTGCCGTCCCCTATGCCAAACTTGAGCTTCTCCA
  GGAAGGTTTTGTTCTCCATGAGATCCAGTGCATTGAACACATCAAACCCTTTCATTTTGG
  CGAGGACAAGGGCGTCGCTCATGAAGTCTATAAGAGGGGTCTGGGTGTGAACGTTGTANA
  AAGAATAAGCAGCTTTGAGACTCTTGTGGGTTGGATGGTTCATGATGGTGGAGGGCAGCG
  TATAAAAGCTCAGGATATCTGTCACCTCTCCGTTTGCGTTCTCCACCACGAAAGTGTCGA
  TGATATTCTCCTGAGGGTAGAACCAGAGCTCCACCTCCTCCTGGCTCATGACGGNCGTAA
  AGTGAAATTGCCTCAAGTACCTGGTGAGGAGCTGGTGCACTACTGGAATGTNCTTTGNTT
  NCATTGGTCGCAGNCCANCTGTCTTGGGAGTCTCTGGGCAGTCGTAAACCTTATGGAGCG
  CTGCATGGNCATATTCTGCTCAGNAGGGAGAACTCACTTCATCAGCTCCGGGGGGTTAGG
  GACGATGCCATACCTGCAGTGCCNACGGNCTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021079
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021079.3, NP_066565.1
• RefSeq Size: 4903 bp
• RefSeq ORF: 1491 bp
• Locus ID: 4836
• UniProt ID: P30419
• Cytogenetics: 17q21.31
• Domains: NMT
• Protein Families: Druggable Genome
• Gene Summary: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008]