NMT1 (NM_021079) Human Untagged Clone

SKU SC113026

NMT1 (untagged)-Human N-myristoyltransferase 1 (NMT1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NMT1
• Synonyms: NMT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113026 sequence for NM_021079 edited (data generated by NextGen Sequencing); ATGGCGGACGAGAGTGAGACAGCAGTGAAGCCGCCGGCACCTCCGCTGCCGCAGATGATG; GAAGGGAACGGGAACGGCCATGAGCACTGCAGCGATTGCGAGAATGAGGAGGACAACAGC; TACAACCGGGGTGGTTTGAGTCCAGCCAATGACACTGGAGCCAAAAAGAAGAAAAAGAAA; CAAAAAAAGAAGAAAGAAAAAGGCAGTGAGACAGATTCAGCCCAGGATCAGCCTGTGAAG; ATGAACTCTTTGCCAGCAGAGAGGATCCAGGAAATACAGAAGGCCATTGAGCTGTTCTCA; GTGGGTCAGGGACCTGCCAAAACCATGGAGGAGGCTAGCAAGCGAAGCTACCAGTTCTGG; GATACGCAGCCCGTCCCCAAGCTGGGCGAAGTGGTGAACACCCATGGCCCCGTGGAGCCT; GACAAGGACAATATCCGCCAGGAGCCCTACACCCTGCCCCAGGGCTTCACCTGGGATGCT; TTGGACCTGGGCGATCGTGGTGTGCTAAAAGAACTGTACACCCTCCTGAATGAGAACTAT; GTGGAAGATGATGACAACATGTTCCGATTTGATTATTCCCCGGAGTTTCTTTTGTGGGCT; CTCCGGCCACCCGGCTGGCTCCCCCAGTGGCACTGTGGGGTTCGAGTGGTCTCAAGTCGG; AAATTGGTTGGGTTCATTAGCGCCATCCCAGCAAACATCCATATCTATGACACAGAGAAG; AAGATGGTAGAGATCAACTTCCTGTGTGTCCACAAGAAGCTGCGTTCCAAGAGGGTTGCT; CCAGTTCTGATCCGAGAGATCACCAGGCGGGTTCACCTGGAGGGCATCTTCCAAGCAGTT; TACACTGCCGGGGTGGTACTACCAAAGCCCGTTGGCACCTGCAGGTATTGGCATCGGTCC; CTAAACCCACGGAAGCTGATTGAAGTGAAGTTCTCCCACCTGAGCAGAAATATGACCATG; CAGCGCACCATGAAGCTCTACCGACTGCCAGAGACTCCCAAGACAGCTGGGCTGCGACCA; ATGGAAACAAAGGACATTCCAGTAGTGCACCAGCTCCTCACCAGGTACTTGAAGCAATTT; CACCTTACGCCCGTCATGAGCCAGGAGGAGGTGGAGCACTGGTTCTACCCCCAGGAGAAT; ATCATCGACACTTTCGTGGTGGAGAACGCAAACGGAGAGGTGACAGATTTCCTGAGCTTT; TATACGCTGCCCTCCACCATCATGAACCATCCAACCCACAAGAGTCTCAAAGCTGCTTAT; TCTTTCTACAACGTTCACACCCAGACCCCTCTTCTAGACCTCATGAGCGACGCCCTTGTC; CTCGCCAAAATGAAAGGGTTTGATGTGTTCAATGCACTGGATCTCATGGAGAACAAAACC; TTCCTGGAGAAGCTCAAGTTTGGCATAGGGGACGGCAACCTGCAGTATTACCTTTACAAT; TGGAAATGCCCCAGCATGGGGGCAGAGAAGGTTGGACTGGTGCTACAATAA; ; Clone variation with respect to NM_021079.3; 487 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021079 unedited; NAAAGGTTCAAATTTAGTATACGACTCATTATAGGCGGCCGCGCCTCGTGCCGAATTCGG; CACGAGGGAGCCCTGCTCTCGCACTCAAGAGGCGGACGAGAGTGAGACAGCAGTGAAGCC; GCCGGCACCTCCGCTGCCGCAGATGATGGAAGGGAACGGGAACGGCCATGAGCACTGCAG; CGATTGCGAGAATGAGGAGGACAACAGCTACAACCGGGGTGGTTTGAGTCCAGCCAATGA; CACTGGAGCCAAAAAGAAGAAAAAGAAACAAAAAAAGAAGAAAGAAAAAGGCAGTGAGAC; AGATTCAGCCCAGGATCAGCCTGTGAAGATGAACTCTTTGCCAGCAGAGAGGATCCAGGA; AATACAGAAGGCCATTGAGCTGTTCTCAGTGGGTCAGGGACCTGCCAAAACCATGGAGGA; GGCTAGCAAGCGAAGCTACCAGTTCTGGGATACGCAGCCCGTCCCCAAGCTGGGCGAAGT; GGTGAACACCCATGGCCCCGTGGAGCCTGACAAGGACAATATCCGCCAGGAGCCCTACAC; CCTGCCCCAGGGCTTCACCTGNGATGCTTTGGACCTGNGCGATCGTGGTGTGCTAAAAGA; ACTGTACACCCTCCTGAATGAGAACTATGTGGAAGATGATGACAACATGTTCCGATTTGA; TTATTCCCCGGAGTTTCTTTTGTGGGCTCTCCGGCCACCCGGCTGGCTCCCCCAGGGACT; GTGTGGGGGTCGAGTGGTCTCAAGTCGGAAATTGTNTGGGNTCATTAGCGCCATCCCAGC; AAACATCCATATCTATGACACAGAGAAGAAGAGGTNGAGATCAACTTCTGTGTGTNCACA; AGAGCTGCNGTCCAGAAGGGTGCTCCAGTTCTGATCCGAGAGATCACCAGCGGGTCACCT; GGAAGGCATCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021079 unedited; GGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTAATATATATATATACTTTTAT; TATTCACCCGTTAACTCCATTATATGCCAATCACGAGCTAGTTTTCAGCAGTTACATTCC; CTTGATCACGGCTGCATGAGGATGTAATTAACTGGAAAACACGGAGACCAGAGACACGAC; CGGAGGTGACGTCACGCAGCCCACGCCATCGCAATACAATTGTCAAGTTCGCTGGCGGTT; TGGTAAAGCCGGTTCAGTTCTGCTCCCTTTGCCAGGGATTCTCACGTGTGTGAAAATTGG; ACCAACAGTGGTGGGGTTCCATTTCCTGGTTCGAATTTTCAGTGGCTTTATCCAGAATCG; CACTGGTGACTGGTTATTGTAGCACCAGTCCAACCTTCTCTGCCCCCATGCTGGGGCATT; TCCAATTGTAAAGGTAATACTGCAGGTTGCCGTCCCCTATGCCAAACTTGAGCTTCTCCA; GGAAGGTTTTGTTCTCCATGAGATCCAGTGCATTGAACACATCAAACCCTTTCATTTTGG; CGAGGACAAGGGCGTCGCTCATGAAGTCTATAAGAGGGGTCTGGGTGTGAACGTTGTANA; AAGAATAAGCAGCTTTGAGACTCTTGTGGGTTGGATGGTTCATGATGGTGGAGGGCAGCG; TATAAAAGCTCAGGATATCTGTCACCTCTCCGTTTGCGTTCTCCACCACGAAAGTGTCGA; TGATATTCTCCTGAGGGTAGAACCAGAGCTCCACCTCCTCCTGGCTCATGACGGNCGTAA; AGTGAAATTGCCTCAAGTACCTGGTGAGGAGCTGGTGCACTACTGGAATGTNCTTTGNTT; NCATTGGTCGCAGNCCANCTGTCTTGGGAGTCTCTGGGCAGTCGTAAACCTTATGGAGCG; CTGCATGGNCATATTCTGCTCAGNAGGGAGAACTCACTTCATCAGCTCCGGGGGGTTAGG; GACGATGCCATACCTGCAGTGCCNACGGNCTTN;
• Restriction Sites: NotI-NotI
• ACCN: NM_021079
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021079.3, NP_066565.1
• RefSeq Size: 4903 bp
• RefSeq ORF: 1491 bp
• Locus ID: 4836
• UniProt ID: P30419
• Cytogenetics: 17q21.31
• Domains: NMT
• Protein Families: Druggable Genome
• Summary: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008]