CDK4 (NM_000075) Human Untagged Clone

CAT#: SC112998

CDK4 (untagged)-Human cyclin-dependent kinase 4 (CDK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK4
• Synonyms: CMM3; PSK-J3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112998 sequence for NM_000075 edited (data generated by NextGen Sequencing)
  ATGGCTACCTCTCGATATGAGCCAGTGGCTGAAATTGGTGTCGGTGCCTATGGGACAGTG
  TACAAGGCCCGTGATCCCCACAGTGGCCACTTTGTGGCCCTCAAGAGTGTGAGAGTCCCC
  AATGGAGGAGGAGGTGGAGGAGGCCTTCCCATCAGCACAGTTCGTGAGGTGGCTTTACTG
  AGGCGACTGGAGGCTTTTGAGCATCCCAATGTTGTCCGGCTGATGGACGTCTGTGCCACA
  TCCCGAACTGACCGGGAGATCAAGGTAACCCTGGTGTTTGAGCATGTAGACCAGGACCTA
  AGGACATATCTGGACAAGGCACCCCCACCAGGCTTGCCAGCCGAAACGATCAAGGATCTG
  ATGCGCCAGTTTCTAAGAGGCCTAGATTTCCTTCATGCCAATTGCATCGTTCACCGAGAT
  CTGAAGCCAGAGAACATTCTGGTGACAAGTGGTGGAACAGTCAAGCTGGCTGACTTTGGC
  CTGGCCAGAATCTACAGCTACCAGATGGCACTTACACCCGTGGTTGTTACACTCTGGTAC
  CGAGCTCCCGAAGTTCTTCTGCAGTCCACATATGCAACACCTGTGGACATGTGGAGTGTT
  GGCTGTATCTTTGCAGAGATGTTTCGTCGAAAGCCTCTCTTCTGTGGAAACTCTGAAGCC
  GACCAGTTGGGCAAAATCTTTGACCTGATTGGGCTGCCTCCAGAGGATGACTGGCCTCGA
  GATGTATCCCTGCCCCGTGGAGCCTTTCCCCCCAGAGGGCCCCGCCCAGTGCAGTCGGTG
  GTACCTGAGATGGAGGAGTCGGGAGCACAGCTGCTGCTGGAAATGCTGACTTTTAACCCA
  CACAAGCGAATCTCTGCCTTTCGAGCTCTGCAGCACTCTTATCTACATAAGGATGAAGGT
  AATCCGGAGTGA
  
  Clone variation with respect to NM_000075.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000075 unedited
  ACCATTAGTATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGTCTATGGTCG
  GGCCCTCTGCGTCCAGCTGCTCCGGACCGAGCTCGGGTGTATGGGGCCGTAGGAACCGGC
  TCCGGGGCCCCGATAACGGGCCGCCCCCACAGCACCCCGGGCTGGCGTGAGGGTCTCCCT
  TGATCTGAGAATGGCTACCTCTCGATATGAGCCAGTGGCTGAAATTGGTGTCGGTGCCTA
  TGGGACAGTGTACAAGGCCCGTGATCCCCACAGTGGCCACTTTGTGGCCCTCAAGAGTGT
  GAGAGTCCCCAATGGAGGAGGAGGTGGAGGAGGCCTTCCCATCAGCACAGTTCGTGAGGT
  GGCTTTACTGAGGCGACTGGAGGCTTTTGAGCATCCCAATGTTGTCCGGCTGATGGACGT
  CTGTGCCACATCCCGAACTGACCGGGAGATCAAGGTAACCCTGGTGTTTGAGCATGTAGA
  CCAGGACCTAAGGACATATCTGGACAAGGCACCCCCACCAGGCTTGCCAGCCGAAACGAT
  CAAGGATCTGATGCGCCAGTTTCTAAGAGGCCTAGATTTCCTTCATGCCAATTGCATCGT
  TCACCGAGATCTGAAGCCAGAGAACATTCTGGTGACAAGTGGTGGAACAGTCAAGCTGGC
  TGACTTTGGCCTGGCCAGAATCTACAGCTACCAGATGGCACTCACACCCGTGGTTGTTAC
  ACTCTGGTACCGAGCTCCCGAAGTTCTTCTGCAGTCCACATATGCCACACCTGTGGACAT
  GTGTAGTGTTGGCTGTATCTTTGCAGAGATGTTCCGTCGGAAGCCTCTCTCCTGTGGAAA
  CTCTGAAACCGACCAGGTGGGGCAAAACTTTGCCCTGANTGGCTGCCTCCACAGTAGACT
  GTCCTCGAGAGTATCCCTGCCCCGTGAAGCCCTTTCCCCCAAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000075 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAATCTATATTCTTT
  TTTTTTTTTTTGACACAGAGTCTTGCTCTGTTGCCCAAGCTGGAGTGCAATGGCATGATC
  TCGGCTCACCGCAACCTCTGCCTCCCGGGTTCAAGCGATCCTCCTGCCTCAGTCTCCCAA
  GTAGCTGGGATTACAGGTGTGCACCACCACGCCTGACTAATTTTGTATTTTTAGTAAAGA
  CAGGGTTTCACCATGTTAGTCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCG
  CGTTGGCCTCCCAACGTGCTGGGATTATAGGCGTGAGCCACCACGCCCCGCCTAAAATCC
  ATATTCAAAGAAGCAATTTCAGTTCCTTTCTAAGCTTTGTCAGTCAAGGGGCTCCACTGA
  CTTCCTAGGCCCTGTAATTTAACCAGTCTTTAAGGTTTTGCAGGAAAGTCCCTTCTTCCA
  AGTGGTTTTTCCAAATCGCACAATGGCAAAGCCAAACCGAGGAAGAAACATTAAAAAAAA
  AAAAAAGACCATTATTTCTTTGTTTCGCTTTTCCTGTATAAAAAAGGACCCCCAATATAA
  AGGTAGGGAAAGGGACAAGAGGGAACATACCCCTTACTGTATAGAAATGGGAAGGAGAAG
  GAAAACCCTCCAAAGGAGAGGTGGGAGGGGACTGTCATTTAAGGCGCAAAAGTATTCTCT
  GTACACAGATGGAGGAAGACCTTCCATATCCTCAGAGATAAAGGCCAAAATTGCCCTTTC
  ACTCGTCACAAGGAAAAGGGAGCTTTTCTTCTTTCCTGGCAGCCACTCCTTTGCTCCTTC
  CGGATACCTTCTCCTTATGTAAATAAGAGGCTGCAAACCCTGAAAAGCATATATTGCTTG
  TGGGGCTTAAAGTCAAAATTTCCCCACAGCTGGGCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000075
• Insert Size: 1960 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000075.2, NP_000066.1
• RefSeq Size: 1474 bp
• RefSeq ORF: 912 bp
• Locus ID: 1019
• UniProt ID: P11802
• Cytogenetics: 12q14.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer, T cell receptor signaling pathway, Tight junction
• Gene Summary: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the full length protein.