ALS2CR1 (NIF3L1) (NM_021824) Human Untagged Clone

CAT#: SC112871

NIF3L1 (untagged)-Human NIF3 NGG1 interacting factor 3-like 1 (S. pombe) (NIF3L1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALS2CR1
• Synonyms: ALS2CR1; CALS-7; MDS015
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112871 sequence for NM_021824 edited (data generated by NextGen Sequencing)
  ATGGATTTGAAGGCTCTCCTTTCTTCCTTGAATGACTTTGCATCCCTCTCGTTTGCTGAG
  AGTTGGGACAATGTTGGATTACTGGTGGAACCAAGCCCACCACATACTGTAAATACACTC
  TTCCTGACCAATGACCTGACTGAGGAAGTGATGGAGGAGGTGCTGCAAAAGAAGGCAGAC
  CTCATTCTCTCCTACCATCCGCCTATCTTCCGACCCATGAAGCGCATAACCTGGAACACA
  TGGAAGGAGCGCCTGGTGATCCGGGCTCTGGAGAACAGAGTCGGTATCTACTCTCCTCAT
  ACAGCCTATGATGCTGCGCCCCAGGGCGTCAACAACTGGTTGGCTAAAGGGCTTGGAGCT
  TGTACCTCCAGGCCCATACATCCTTCCAAAGCTCCCAACTACCCTACAGAGGGAAACCAC
  CGAGTAGAATTCAACGTTAACTACACCCAAGACCTGGACAAAGTCATGTCTGCAGTGAAA
  GGAATTGACGGTGTTTCTGTCACTTCTTTTTCTGCTAGGACTGGTAATGAGGAACAAACA
  CGGATTAATCTGAATTGTACTCAGAAGGCTTTGATGCAGGTGGTAGATTTTCTTTCCCGG
  AACAAACAACTTTATCAGAAGACGGAAATTCTGTCACTGGAGAAGCCTTTGCTTCTACAT
  ACTGGAATGGGACGGTTATGCACACTGGATGAATCTGTCTCCCTGGCAACCATGATTGAT
  CGAATAAAAAGACACCTAAAACTATCTCATATTCGCTTAGCCCTTGGGGTGGGGAGAACC
  TTAGAGTCTCAAGTCAAAGTCGTGGCCCTGTGTGCTGGTTCTGGGAGCAGCGTTCTGCAG
  GGTGTTGAGGCTGACCTTTACCTCACAGGTGAGATGTCCCATCATGATATTTTGGATGCT
  GCTTCCCAAGGAATAAATGTCATCCTCTGTGAACACAGCAACACTGAACGAGGCTTTCTT
  TCTGACCTTCGAGATATGCTGGATTCTCACTTGGAGAATAAGATAAATATTATCCTATCA
  GAGACTGACAGGGACCCTCTTCAGGTGGTATAA
  
  Clone variation with respect to NM_021824.3
  890 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021824 unedited
  NNCGTCGGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCGATTT
  AAAAACCCGCGAGTGTAGTTGTGACCTTCGCGGTAGGTGCCGGTTGGGGCCGGCTGTGAT
  TGTTATCTTGGTGCTGCAGAGGACAGCAGAAGAGGAGATTGGGTCAGAAAACTGCCCTGC
  CGCACCAGAGCACAGCGCACTAGTGGGACAGGGGTCCTGACTCAGACTTAACTGGCTGTG
  TCTCGTGGTTTTTCACTGTCCTGGAAAAGGCCTGAAGTGGCACTGAAATGAGGCATAGAT
  GAGGTAACGTCGAGGGAGCGGTTTGCAGGTTGCAAAGGGGCCCTACTGATGCTGCTTAAT
  AGTAAACAGTGCTTACTCTAGGACGGGAGTTGTTTCCAGCTTTACGTGTAAATCTCGCGA
  TATTGTGGCCTGGGCACTATTTCCATTAAGGAGGAAACTGCACAGGATAAGTCATTTACC
  TGCTGTTGCACAGCTCTGGCTCTAGGATTCAGGCTGGCTTCAGAGTCGGTGCTCTTAACC
  GCCATACTATGTTGCCTTCTGTTNNNCGTTGTTGACTGTGGCCATGTATNTTNNGGCAGT
  TTCCTCATCTGTAACAGTAAATAACTCAAAACCTGCATGATGGCACTAAAGGGTTTCACT
  TTCGAGCTTGCCAGATGGTAGAAATGCTGGAGAAATGGAGTCTTATGAGGACCTTTAGAA
  GATCTTTCAAGGCCGGNACGCAGTGGCTCACCCCTGTAATCCCGGTCTCTTTTGGAGGCC
  GAGGCAGGGTTGAATCACTTGGGGCCCGGAAGTTTGAGACCAGCCTGGNGCACGTGGCGA
  ATCCCCGTCTCTGCTAGAAACAAATAGCCGGGGGGGGGGGGGGGTTTAGCGCCTGGATCC
  CACTGTCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021824 unedited
  GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTTAAGTAAACAGATTTTATTC
  AATCTTTCCTAGAGTTTATAATGAACATTTGTTATTTAATATGGTAGTTATATTACAGTT
  TTACCTTACGAGCGATAGAACATTTGGTGAATAAGATTAACAAACCGGAAATGATGATAC
  CCTCGAAGACACTCTCTGGAAGCACACCAGTCCCACTGACTCATGTTACAAATTTAAGTT
  GGCCTCCAGCTGATTTGTAGAATGTGTTATCCTGATGTTTCTGCAATTATACCACCTGAA
  GAGGGTCCCTGTCAGTCTCTGATAGGATAATATTTATCTTATTCTCCAAGTGAGAATCCA
  GCATATCTCGAAGGTCAGAAAGAAAGCCTCGTTCAGTGTTGCTGGGTTCACCACAGGATG
  ACATTTATTCCTTGGGAAGCAGCATCCAAAATATTATGACGGGACATCCCCCCCCGCGAT
  GTAAAGGGCAGCCTTAACACCCTGGAGAACGCTGCTCCCAGAACCAGCACACAGGGCCCC
  GACTTTGACTTGCCACTTCCGGCTCTGCCCCCCCCAGGGCTACCGAATATGAGATCGGCG
  AGGCGCCGCTCTATTCGCCACCCCGGGTGCCGCCGGGNACGAACCCAGCCCGGGCGGTAC
  CGTGCCTTGCCACTGACGACCGAAGGCTCGGCCGGGACGATTAGCAGCGTGCGACGCGTG
  AGCGCCGCGGGAGAAGTATTAATTGTCGGCTGGCCGGTCCCCCCCAGGTGGTCCCACCGA
  GGAGCCGCGTCCGCCCATCGAATCGCGGGACGAGGGAAGGGCTGGCGCGCGAGTGCGACC
  GCGGCGGGCGGCAGAGTACTATTATAGTGTCCCCGCCCCCCGGGTTCCCGCCGCGCCCCC
  GCCCCCCCGTCAAGTGCGCCGGGGCCGCCCACGCTGTGTGTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021824
• Insert Size: 3970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021824.2, NP_068596.2
• RefSeq Size: 1606 bp
• RefSeq ORF: 1053 bp
• Locus ID: 60491
• UniProt ID: Q9GZT8
• Cytogenetics: 2q33.1
• Domains: NIF3, DUF34
• Gene Summary: May function as a transcriptional corepressor through its interaction with COPS2, negatively regulating the expression of genes involved in neuronal differentiation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream in-frame translation initiation codon, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform.