XLF (NHEJ1) (NM_024782) Human Untagged Clone

CAT#: SC112059

NHEJ1 (untagged)-Human nonhomologous end-joining factor 1 (NHEJ1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NHEJ1
• Synonyms: XLF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024782, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGAACTGGAGCAAGGCCTGTTGATGCAGCCATGGGCGTGGCTACAGCTTGCAGAGAACTCCCTCT
  TGGCCAAGGTTTTTATCACCAAGCAGGGCTATGCCTTGTTGGTTTCAGATCTTCAACAGGTGTGGCATGA
  ACAGGTGGACACTAGTGTGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGGCTCACTGCTCCTCCTGCA
  GCTTTCCTCTGTCATTTGGATAATCTCCTTCGCCCATTGTTGAAGGACGCTGCTCACCCTAGCGAAGCTA
  CCTTCTCCTGTGATTGTGTGGCAGATGCACTGATTCTACGGGTGCGAAGTGAGCTCTCTGGCCTCCCCTT
  CTATTGGAATTTCCACTGCATGCTAGCTAGTCCTTCCCTGGTCTCCCAACATTTGATTCGTCCTCTGATG
  GGCATGAGTCTGGCATTACAGTGCCAAGTGAGGGAGCTAGCAACGTTACTTCATATGAAAGACCTAGAGA
  TCCAAGACTACCAGGAGAGTGGGGCTACGCTGATTCGAGATCGATTGAAGACAGAACCATTTGAAGAAAA
  TTCCTTCTTGGAACAATTTATGATAGAGAAACTGCCAGAGGCATGCAGCATTGGTGATGGAAAGCCCTTT
  GTCATGAATCTGCAGGATCTGTATATGGCAGTCACCACACAAGAGGTCCAAGTGGGACAGAAGCATCAAG
  GCGCTGGAGATCCTCATACCTCAAACAGTGCTTCCCTGCAAGGAATCGATAGCCAATGTGTAAACCAGCC
  AGAACAACTGGTCTCCTCAGCCCCAACCCTCTCAGCACCTGAGAAAGAGTCCACGGGTACTTCAGGCCCT
  CTGCAGAGACCTCAGCTGTCAAAGGTCAAGAGGAAGAAGCCAAGGGGTCTCTTCAGTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024782 unedited
  NGGTGCGGAATAGTATACGATTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGATGAT
  GGAAGAACTGGAGCAAGGCCTGTTGATGCAGCCATGGGCGTGGCTACAGCTTGCAGAGAA
  CTCCCTCTTGGCCAAGGTTTTTATCACCAAGCAGGGCTATGCCTTGTTGGTTTCAGATCT
  TCAACAGGTGTGGCATGAACAGGTGGACACTAGTGTGGTCAGCCAGCGAGCCAAGGAGCT
  GAACAAGCGGCTCACTGCTCCTCCTGCAGCTTTCCTCTGTCATTTGGATAATCTCCTTCG
  CCCATTGCTGAAGGACGCTGCTCACCCTAGCGAAGCTACCTTCTCCTGTGATTGTGTGGC
  AGATGCACTGATTCTACGGGTGCGAAGTGAGCTCTCTGGCCTCCCCTTCTATTGGAATTT
  CCACTGCATGCTAGCTAGTCCTTCCCTGGTCTCCCAACATTTGATTCGTCCTCTGATGGG
  CATGAGTCTGGCATTACAGTGCCAAGTGAGGGAGCTAGCAACGTTACCTTATATGAAAGA
  CCTAGAGATCCAAGACTACCAGGAGAGTGGCGCTACGCTGATTCGCGATCGATTGCAGAC
  AGAACCCTTTGAAGAAAATTCCTTCTTGGAACCAATTATGATACAAGAACCTGCCCGAGG
  CCATGCAGCATTTGTTGATGGAAAGCCCCTTTGTCATGAATCTGCAGGATCCTGCCTACG
  GCAGTCACCCCCACAAGAGGCCCAAGTGGGACAGAAGCCTCAAGGCGGCTGGAGACCCCA
  TACCTCAACCAGGCTTTCCCTGCCGGATCCCTCGCCACTGGGAAACCAGCCGAAACACTG
  TCTCCTTATTCCACCCTTTAATACCTGATAAAGAGCCCCGGTACTTCAGCCTTCTGCCAA
  ACCCCACTGCCAAGGCCAGAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024782
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024782.1, NP_079058.1
• RefSeq Size: 2037 bp
• RefSeq ORF: 900 bp
• Locus ID: 79840
• UniProt ID: Q9H9Q4
• Cytogenetics: 2q35
• Protein Pathways: Non-homologous end-joining
• Gene Summary: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]