DDX39 (DDX39A) (NM_005804) Human Untagged Clone

SKU SC111745

DDX39A (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A (DDX39A), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DDX39
• Synonyms: BAT1; BAT1L; DDX39; DDXL; URH49
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005804 edited; GAATTCGGCACGAGGCCGAAGTTGGAAGTGTCTCTTAGCAGCGCGCGGAGAAGAACGGGG; AGCCAGCATCATGGCAGAACAGGATGTGGAAAACGATCTTTTGGATTACGATGAAGAGGA; AGAGCCCCAGGCTCCTCAAGAGAGCACACCAGCTCCCCCTAAGAAAGACATCAAGGGATC; CTACGTTTCCATCCACAGCTCTGGCTTCCGGGACTTTCTGCTGAAGCCGGAGCTCCTGCG; GGCCATCGTGGACTGTGGCTTTGAGCATCCTTCTGAGGTCCAGCATGAGTGCATTCCCCA; GGCCATCCTGGGCATGGACGTCCTGTGCCAGGCCAAGTCCGGGATGGGCAAGACAGCGGT; CTTCGTGCTGGCCACCCTACAGCAGATTGAGCCTGTCAACGGACAGGTGACGGTCCTGGT; CATGTGCCACACGAGGGAGCTGGCCTTCCAGATCAGCAAGGAATATGAGCGCTTTTCCAA; GTACATGCCCAGCGTCAAGGTGTCTGTGTTCTTCGGTGGTCTCTCCATCAAGAAGGATGA; AGAAGTGTTGAAGAAGAACTGTCCCCATGTCGTGGTGGGGACCCCGGGCCGCATCCTGGC; GCTCGTGCGGAATAGGAGCTTCAGCCTAAAGAATGTGAAGCACTTTGTGCTGGACGAGTG; TGACAAGATGCTGGAGCAGCTGGACATGCGGCGGGATGTGCAGGAGATCTTCCGCCTGAC; ACCACACGAGAAGCAGTGCATGATGTTCAGCGCCACCCTGAGCAAGGACATCCGGCCTGT; GTGCAGGAAGTTCATGCAGGATCCCATGGAGGTGTTTGTGGACGACGAGACCAAGCTCAC; GCTGCACGGCCTGCAGCAGTACTACGTCAAACTCAAAGACAGTGAGAAGAACCGCAAGCT; CTTTGATCTCTTGGATGTGCTGGAGTTTAACCAGGTGATAATCTTCGTCAAGTCAGTGCA; GCGCTGCATGGCCCTGGCCCAGCTCCTCGTGGAGCAGAACTTCCCGGCCATCGCCATCCA; CCGGGGCATGGCCCAGGAGGAGCGCCTGTCACGCTATCAGCAGTTCAAGGATTTCCAGCG; GCGGATCCTGGTGGCCACCAATCTGTTTGGCCGGGGGATGGACATCGAGCGAGTCAACAT; CGTCTTTAACTACGACATGCCTGAGGACTCGGACACCTACCTGCACCGGGTGGCCCGGGC; GGGTCGCTTTGGCACCAAAGGCCTAGCCATCACTTTTGTGTCTGACGAGAATGATGCCAA; AATCCTCAATGACGTCCAGGACCGGTTTGAAGTTAATGTGGCAGAACTTCCAGAGGAAAT; CGACATCTCCACATACATCGAGCAGAGCCGGTAACCACCACGTGCCAGAGCCGCCCACCC; GGAGCCGCCCGCATGCAGCTTCACCTCCCCTTTCCAGGCGCCACTGTTGAGAAGCTAGAG; ATTGTATGAGAATAAACTTGTTATTATGGAAGCCTGAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005804 unedited; GGTTCANATTTGTATACGACTCATATAGGCGGCCGCGCAATTCGCACGAGGGCCGAAGTT; GGAAGTGTCTCTTAGCAGCGCGCGGAGAAGAACGGGGAGCCAGCATCATGGCAGAACAGG; ATGTGGAAAACGATCTTTTGGATTACGATGAAGAGGAAGAGCCCCAGGCTCCTCAAGAGA; GCACACCAGCTCCCCCTAAGAAAGACATCAAGGGATCCTACGTTTCCATCCACAGCTCTG; GCTTCCGGGACTTTCTGCTGAAGCCGGAGCTCCTGCGGGCCATCGTGGACTGTGGCTTTG; AGCATCCTTCTGAGGTCCAGCATGAGTGCATTCCCCAGGCCATCCTGGGCATGGACGTCC; TGTGCCAGGCCAAGTCCGGGATGGGCAAGACAGCGGTCTTCGTGCTGGCCACCCTACAGC; AGATTGAGCCTGTCAACGGACAGGTGACGGTCCTGGTCATGTGCCACACGAGGGAGCTGG; CCTTCCAGATCAGCAAGGAATATGAGCGCTTTTCCAAGTACATGCCCAGCGTCAAGGTGT; CTGTGTTCTTCGGTGGTCTCTCCATCAAGAAGGATGAAGAAGTGTTGAAGAAGAACTGTC; CCCATGTCGTGGTGGGGACCCCGGGCCGCATCCTGGCGCTCGTGCGGAATAGGAGCTTCA; GCCTAAAGAATGTGAAGCACTTTGTGCTGGACGAGTGTGACAAGATGCTGGAGCAGCTGG; ACATGCGGCGGGATGTGCANGAGATCTTCCGCCTGACACCACACGAGAAGCAGTGCATGA; TGTTCAGCGCCACCCTGAGCAGGACATCCCGCCTGTGTGCAGGAAGTTCATGCACGATCC; CATGGAAGTTGTTTTGTGCACGACCAGCACCAGCTCACGCTGCACGGCCTGCAGCAGTAC; TACGTCAAACTTCAAGACG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005804 unedited; ACTATGAACCCGCGGCCGCAATCTANNGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGCTTCCATAAAAACAAGTTTT; TTTTAATACAATTTTTACTTTTTAAACAGGGGCCCCTGAAAAGGGGAGGTAAACCTGCTT; GCGGGCGGTTCCGGGGGGGCGGTTTTGGCCCGTGGGGGTTCCCGGCTTTGCTCAATGTTT; GGGGAAATTCCAATTTCCTTTGAAAGTTTTGCCACATTAACTTAAAACCGGCCCTGGACG; TTATTGAGGATTTTGGCTTTTTTTTCGAAAAACACAAAAGTGATGGCTAGGCCTTTGGGG; CCAAAACAACCCCCCCGGCCCACCCGGGGCAGGTAGGGGCCCAAACCCTCAGGCTTTTCG; TATTTAAAAACAATGTTACCCCCCTCGATGCCCATCCCCCGGCCAAACAAATTGGGGGCC; CCCAAAACCCCCCCCTGAAAATCCTGAACCTGCTAAAACGTAACAGGCCCTTCTTCTGGC; CCTTCCCCGGGGGGAGGCCAATGCCCGAAAATTTTGCTCCCCAAAGAACTGGGCAGGGCC; ATTCCCCCTTTTCTTTTTTTAACAAAAATATCCCCCGTTAACCTCCCCCCCCCAAAAATC; AAAAAGTTGGGGGGTTTTTCCATGTCTTTAAATTTACACCAACCCTCTCCACCCCCCCCC; ATAAAACTTTTTTTTTCTTTCCCAACAAACCTCCTTGGGGCCCCCCATAACACCCTCCCA; AAAGCCGCGATTTCTTCCTCCAGGGGCTTAAACAAAAATCCCTTCTTTTGGGGTGTCGTC; AAAAAAACTTTCCTCACCCCAGTTCTAATAACTCAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_005804
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005804.2, NP_005795.2
• RefSeq Size: 1534 bp
• RefSeq ORF: 1284 bp
• Locus ID: 10212
• UniProt ID: O00148
• Cytogenetics: 19p13.12
• Domains: DEAD, helicase_C
• Summary: This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is thought to play a role in the prognosis of patients with gastrointestinal stromal tumors. A pseudogene of this gene is present on chromosome 13. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]; Transcript Variant: This variant (1) represents the longer transcript.