Hsp60 (HSPD1) (NM_002156) Human Untagged Clone

CAT#: SC111640

HSPD1 (untagged)-Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Hsp60
• Synonyms: CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111640 sequence for NM_002156 edited (data generated by NextGen Sequencing)
  ATGCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTACTGGCTCCT
  CATCTCACTCGGGCTTATGCCAAAGATGTAAAATTTGGTGCAGATGCCCGAGCCTTAATG
  CTTCAAGGTGTAGACCTTTTAGCCGATGCTGTGGCCGTTACAATGGGGCCAAAGGGAAGA
  ACAGTGATTATTGAGCAGAGTTGGGGAAGTCCCAAAGTAACAAAAGATGGTGTGACTGTT
  GCAAAGTCAATTGACTTAAAAGATAAATACAAAAACATTGGAGCTAAACTTGTTCAAGAT
  GTTGCCAATAACACAAATGAAGAAGCTGGGGATGGCACTACCACTGCTACTGTACTGGCA
  CGCTCTATAGCCAAGGAAGGCTTCGAGAAGATTAGCAAAGGTGCTAATCCAGTGGAAATC
  AGGAGAGGTGTGATGTTAGCTGTTGATGCTGTAATTGCTGAACTTAAAAAGCAGTCTAAA
  CCTGTGACCACCCCTGAAGAAATTGCACAGGTTGCTACGATTTCTGCAAACGGAGACAAA
  GAAATTGGCAATATCATCTCTGATGCAATGAAAAAAGTTGGAAGAAAGGGTGTCATCACA
  GTAAAGGATGGAAAAACACTGAATGATGAATTAGAAATTATTGAAGGCATGAAGTTTGAT
  CGAGGCTATATTTCTCCATACTTTATTAATACATCAAAAGGTCAGAAATGTGAATTCCAG
  GATGCCTATGTTCTGTTGAGTGAAAAGAAAATTTCTAGTATCCAGTCCATTGTACCTGCT
  CTTGAAATTGCCAATGCTCACCGTAAGCCTTTGGTCATAATCGCTGAAGATGTTGATGGA
  GAAGCTCTAAGTACACTCGTCTTGAATAGGCTAAAGGTTGGTCTTCAGGTTGTGGCAGTC
  AAGGCTCCAGGGTTTGGTGACAATAGAAAGAACCAGCTTAAAGATATGGCTATTGCTACT
  GGTGGTGCAGTGTTTGGAGAAGAGGGATTGACCCTGAATCTTGAAGACGTTCAGCCTCAT
  GACTTAGGAAAAGTTGGAGAGGTCATTGTGACCAAAGACGATGCCATGCTCTTAAAAGGA
  AAAGGTGACAAGGCTCAAATTGAAAAACGTATTCAAGAAATCATTGAGCAGTTAGATGTC
  ACAACTAGTGAATATGAAAAGGAAAAACTGAATGAACGGCTTGCAAAACTTTCAGATGGA
  GTGGCTGTGCTGAAGGTTGGTGGGACAAGTGATGTTGAAGTGAATGAAAAGAAAGACAGA
  GTTACAGATGCCCTTAATGCTACAAGAGCTGCTGTTGAAGAAGGCATTGTTTTGGGAGGG
  GGTTGTGCCCTCCTTCGATGCATTCCAGCCTTGGACTCATTGACTCCAGCTAATGAAGAT
  CAAAAAATTGGTATAGAAATTATTAAAAGAACACTCAAAATTCCAGCAATGACCATTGCT
  AAGAATGCAGGTGTTGAAGGATCTTTGATAGTTGAGAAAATTATGCAAAGTTCCTCAGAA
  GTTGGTTATGATGCTATGGCTGGAGATTTTGTGAATATGGTGGAAAAAGGAATCATTGAC
  CCAACAAAGGTTGTGAGAACTGCTTTATTGGATGCTGCTGGTGTGGCCTCTCTGTTAACT
  ACAGCAGAAGTTGTAGTCACAGAAATTCCTAAAGAAGAGAAGGACCCTGGAATGGGTGCA
  ATGGGTGGAATGGGAGGTGGTATGGGAGGTGGCATGTTCTAA
  
  Clone variation with respect to NM_002156.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002156 unedited
  TTTAGGTTTTGTAATACGACTTACTATAGGGCGGCCGACGAATTCGCACGAGGGTCTCGC
  CGAGCGCACGCCTTGCCGCCGCCCCGCAGAATGCTTCGGTTACCCACAGTCTTTCGCCAG
  ATGAGACCGGTGTCCAGGGTACTGGCTCCTCATCTCACTCGGGCTTATGCCAAAGATGTA
  AAATTTGGTGCAGATGCCCGAGCCTTAATGCTTCAAGGTGTAGACCTTTTAGCCGATGCT
  GTGGCCGTTACAATGGGGCCAAAGGGAAGAACAGTGATTATTGAGCAGAGTTGGGGAAGT
  CCCAAAGTAACAAAAGATGGTGTGACTGTTGCAAAGTCAATTGACTTAAAAGATAAATAC
  AAAAACATTGGAGCTAAACTTGTTCAAGATGTTGCCAATAACACAAATGAAGAAGCTGGG
  GATGGCACTACCACTGCTACTGTACTGGCACGCTCTATAGCCAAGGAAGGCTTCGAGAAG
  ATTAGCAAAGGTGCTAATCCAGTGGAAATCAGGAGAGGTGTGATGTTAGCTGTTGATGCT
  GTAATTGCTGAACTTAAAAAGCAGTCTAAACCTGTGACCACCCCTGAAGAAATTGCACAG
  GTTGCTACGATTTCTGCAAACGGAGACCAAGAAATTGGCAATATCATCTCTGATGCAATG
  AANAAAGTTGGAAGAAAGGGTGTCATCACAGTAAAGGATGGAAAAACACTGAATGATGAA
  TTAGAAATTATTGAAGGCATNGAGTTTGATCGAGGCTATNATTCCCATACTTTATTAATA
  CATCANAAGGTCAGAAATGTGAATTNCCAGATGCCTATGTTCTGNTGAGTGAAAAGAAAT
  TTCTAGTATCCAGTCCATTGTACCTGCTNCTGAAATTGCAATGCTNACCGNTAGCCTTGG
  NTCATNATCGCTGAAGAATGTGATGGAGAAAGCTCTAGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002156 unedited
  GCACTTCCAGGGCCGGAATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGT
  TCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTA
  ACTTTAAACAAATTTTTATTACACAAAGGTTGTCACATAATTGGATACTTCTCTACTTTG
  TACACAATTATTCTCACTCTCCACAGAAAGGCTGCTTAACTTCTCATCTGGTGGTGGCAA
  GCACTAAAATCCTGATTTTAACAGAATAGTAGTAAAAATGCCTCAGTGATTTAAGTTGAA
  AGCAGTACACTGGTACATGGCTCTTGTACCCAGTATCAGGAATGTACAAATGTTTTTTAT
  TCAAAAATACAAAATAAATTATCTGTAGGCATGGACAATGACAGCAGTAAACCATTATAT
  ATTTTGTCAACTGAAACCAGTAACTGATGGTTATAGTGATTTTCAGCCAGCCTTTTTCTT
  CATTTTCTCCAACTGACTTCTCTGAAGTTATTGGTGAGGAACACTGCCTTGGGCTTCCTG
  TCACAGTTCATTAATAAAGGTAAAGCACTAGTCTAGGAGTTAGAACATGCCACCTCCCAT
  ACCACCTCCCATTCCACCCATTGCACCCATTCCAGGGTCCTTCTCTTCTTTAGGAATTTC
  TGTGACTACAACTTCTGCTGTAGTTAACAGAGAGGCCACACCAGCAGCATCCAATAAAGC
  AGTTCTCACAACCTTTGNTGGGTCAATGATTCCTTTTTCCACCATATTCACAAAATCTNC
  AGCCATAGCATCATAACCCACTTCTGAGGAACTTTGCATAANTTTCTCACTATCAAAGAT
  CCTTTCACACCTGCATTCTTAGCATGGNCATTGCTGGAAATTTGGAGTGTCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002156
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002156.4, NP_002147.2
• RefSeq Size: 2339 bp
• RefSeq ORF: 1722 bp
• Locus ID: 3329
• UniProt ID: P10809
• Cytogenetics: 2q33.1
• Domains: cpn60_TCP1
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: RNA degradation, Type I diabetes mellitus
• Gene Summary: This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.