DNMT3B (NM_006892) Human Untagged Clone

CAT#: SC111622

DNMT3B (untagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNMT3B
• Synonyms: ICF; ICF1; M.HsaIIIB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006892 edited
  ATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCG
  ATCCTCGTCAACGGGGCCTGCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCT
  ATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTG
  TCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGAT
  GGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGC
  CCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCC
  CCACGTTCCACCCGAGGCCGGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTC
  CCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCC
  CCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACG
  CCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATG
  GAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGG
  AAGGAGTTTGGAATAGGGGACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCC
  GCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTGG
  GTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTG
  GGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAATAAGCTCGTCTCCTATCGAAAA
  GCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGC
  CCTGGAGACTCATTGGAGGACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTC
  AAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCGA
  AGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAG
  ACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCGAGAACAAATG
  GCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGG
  AAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGAT
  CGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTG
  TGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGT
  GTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAG
  CCCTGGAGCTGCTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAG
  GACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACACGGGGCTTGAATACGAAGCC
  CCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTG
  TTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAG
  TACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGG
  AATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGC
  CCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCC
  AGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAAT
  TACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTA
  GCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATG
  ATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCC
  GGGATGAACAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGT
  GGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTC
  GCCCCTCTGAAGGACTACTTTGCATGTGAATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006892 unedited
  GCATATGTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCCGACCCGCGGC
  TCCGCCGCCCAGCCGCGCCCCAGCCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCA
  GGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGG
  CCTGCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGA
  TCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTGTCCAGTCTGCTAAGCT
  ACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAG
  TCATGCCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTC
  GAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAG
  GCCGGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCC
  TGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCCCCTCCCAGCTCTTACC
  TTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTA
  CCCCCTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGG
  AGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAG
  GGGACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCATGGTGGTGTCTTG
  GAAGGCCACCTNCAGCGACAGGCTATGTCTGCATGNCGTGGTTCAGTGGTTTGGCGATGG
  GCAGTCTNCGAGGTCTCTGAGACAACTGGTGACTTGGGGCTGTTCACCAGCACTTTATTN
  GGCCACTTTCATAGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006892 unedited
  ACGACTNTGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTAGACAA
  ATACTGATTTTAATTAAACATAAGGTAAACTCTAGGCATCCGTCATCTTTCAGCCTAAAA
  ATTAGCAAAAACTGTTGAAACAAGGCACAGTTTTTTCCCCATATTTGTTACGTCGTGGCT
  CCAGTTACAAAAAAATTTTAATGAAAACGTTAAACATAAAAATAGAAGTTTGAGATTTTA
  AAAAGTGTATAAAAAGCCCCACAAAACTTGTCAACGTTGTTCCTTATTCTACAAAATAGC
  ACCAGTAAGAAGAGTAAAAGGTGTTAAAAACCATTATGACAGCATTTCTGAAATGCAGCT
  TGTCTGAATTCCCGTTCTCCCTAAAAACGACTTCTTATGGAATAAAAAAGGATTAAAAAA
  TCTCCAAAGGGAGCACCGAGCTTTGCAGTTTTCCCTGTCATCTCTCAGATGTGGGGAAGG
  TATGAGAAATGTATGTCTGTCCCTGACTGCTGTCACTGCCTCTGAGTTTAGTAAAAAGAT
  GAGAAATGAGGGTAGCAGACTTCTCATCTGGGGACCTGTGCCTGTGGAGGGTAGGTCTCC
  TGGAGAGGGAATGGGTTTGAGGGGGTGTCTTATCTAAGCTCCTTGCTTCACACTCCTCTC
  CTAGGGAAGAGGGAGATACGGAGGAGCCATACTTGTCTACAAAATATATGGCAGCTTATA
  TTATAGAACTCAGCACACCCCTTCCTGAGCTCCCCAGAGGGTTCTATTGCAGTCACGGGG
  AGGGATTTAGCTAACAGTGGCCCCTGCCTTAGGGTTTGTGGGGGAAAAAATAGCCCTGAT
  GTGATGATGACGTCATCCCTGCTGAAATATCATCATTGTGGAAATACTCTATAAGTCCCG
  TGCAGACTCCAGCCCTGNATTTATGCANGNGCAATGTGCCTGGTAAAGACAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006892
• Insert Size: 3800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006892.3, NP_008823.1
• RefSeq Size: 4353 bp
• RefSeq ORF: 2562 bp
• Locus ID: 1789
• UniProt ID: Q9UBC3
• Cytogenetics: 20q11.21
• Domains: PWWP, DNA_methylase
• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).