Caspase 4 (CASP4) (NM_001225) Human Untagged Clone

CAT#: SC111600

CASP4 (untagged)-Human caspase 4, apoptosis-related cysteine peptidase (CASP4), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Caspase 4
• Synonyms: ICE(rel)II; ICEREL-II; ICH-2; Mih1; Mih1/TX; TX
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111600 sequence for NM_001225 edited (data generated by NextGen Sequencing)
  ATGGCAGAAGGCAACCACAGAAAAAAGCCACTTAAGGTGTTGGAATCCCTGGGCAAAGAT
  TTCCTCACTGGTGTTTTGGATAACTTGGTGGAACAAAATGTACTGAACTGGAAGGAAGAG
  GAAAAAAAGAAATATTACGATGCTAAAACTGAAGACAAAGTTCGGGTCATGGCAGACTCT
  ATGCAAGAGAAGCAACGTATGGCAGGACAAATGCTTCTTCAAACCTTTTTTAACATAGAC
  CAAATATCCCCCAATAAAAAAGCTCATCCGAATATGGAGGCTGGACCACCTGAGTCAGGA
  GAATCTACAGATGCCCTCAAGCTTTGTCCTCATGAAGAATTCCTGAGACTATGTAAAGAA
  AGAGCTGAAGAGATCTATCCAATAAAGGAGAGAAACAACCGCACACGCCTGGCTCTCATC
  ATATGCAATACAGAGTTTGACCATCTGCCTCCGAGGAATGGAGCTGACTTTGACATCACA
  GGGATGAAGGAGCTACTTGAGGGTCTGGACTATAGTGTAGATGTAGAAGAGAATCTGACA
  GCCAGGGATATGGAGTCAGCGCTGAGGGCATTTGCTACCAGACCAGAGCACAAGTCCTCT
  GACAGCACATTCTTGGTACTCATGTCTCATGGCATCCTGGAGGGAATCTGCGGAACTGTG
  CATGATGAGAAAAAACCAGATGTGCTGCTTTATGACACCATCTTCCAGATATTCAACAAC
  CGCAACTGCCTCAGTCTGAAGGACAAACCCAAGGTCATCATTGTCCAGGCCTGCAGAGGT
  GCAAACCGTGGGGAACTGTGGGTCAGAGACTCTCCAGCATCCTTGGAAGTGGCCTCTTCA
  CAGTCATCTGAGAACCTAGAGGAAGATGCTGTTTACAAGACCCACGTGGAGAAGGACTTC
  ATTGCTTTCTGCTCTTCAACGCCACACAACGTGTCCTGGAGAGACAGCACAATGGGCTCT
  ATCTTCATCACACAACTCATCACATGCTTCCAGAAATATTCTTGGTGCTGCCACCTAGAG
  GAAGTATTTCGGAAGGTACAGCAATCATTTGAAACTCCAAGGGCCAAAGCTCAAATGCCC
  ACCATAGAACGACTGTCCATGACAAGATATTTCTACCTCTTTCCTGGCAATTGA
  
  Clone variation with respect to NM_001225.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001225 unedited
  NGGGTGCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAACGCTG
  TAAAAAAGACAGAGGCTGTTCCCTATGGCAGAAGGCAACCACAGNAAAAAAGCCACTTAA
  GGTGTTGGAATCCCTGGGCAAAGATTTCCTCACTGGTGTTTTGGATAACTTGGTGGAACA
  AAATGTACTGAACTGGAAGGAAGAGGAAAAAAAGAAATATTACGATGCTAAAACTGAAGA
  CAAAGTTCGGGTCATGGCAGACTCTATGCAAGAGAAGCAACGTATGGCAGGACAAATGCT
  TCTTCAAACCTTTTTTAACATAGACCAAATATCCCCCAATAAAAAAGCTCATCCGAATAT
  GGAGGCTGGACCACCTGAGTCAGGAGAATCTACAGATGCCCTCAAGCTTTGTCCTCATGA
  AGAATTCCTGAGACTATGTAAAGAAAGAGCTGAAGAGATCTATCCAATAAAGGAGAGAAA
  CAACCGCACACGCCTGGCTCTCATCATATGCAATACAGAGTTTGACCATCTGCCTCCGAG
  GAATGGAGCTGACTTTGACATCACAGGGATGAAGGAGCTACTTGAGGGTCTGGACTATAG
  TGTAGATGTAGAAGAGAATCTGACAGCCAGGGATATGGAGTCAGCGCTGAGGGCATTTGC
  TACCAGACCAGAGCACAAGTCCTCTGACAGCACATTCTTGGTACTCATGTCTCATGGCAT
  CCTGGAGGGAATCTGCGGAACTGTGCATGATGAGAAAAAACCAGATGTGCTGCTTTATGA
  CACCATCTTCCAGATATTCAACAAACCGCACTGCCTCAGTCTGAAGGACAAACCCAAGTC
  ATCATTGTCCAGGCCTGCAGAAGTGCANACCGTGGGGAACNNTGTGGTCAGAGACTCTTC
  AGCATCCTTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001225 unedited
  NCCTCTGTGNACCGCGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTGTCTTCACT
  TTTATTGAAATACAAAATGTTAAATATGCAAGCTGTACTAATGAAGGTGCTCCTTGAAGT
  TGATTAAGGAGGGCTGGGCTGCTTGTGGCTTCCATTTTCAATTGCCAGGAAAGAGGTAGA
  AATATCTTGTCATGGACAGTCGTTCTATGGTGGGCATTTGAGCTTTGGCCCTTGGAGTTT
  CAAATGATTGCTGTACCTTCCGAAATACTTCCTCTAGGTGGCAGCACCAAGAATATTTCT
  GGAAGCATGTGATGAGTTGTGTGATGAAGATAGAGCCCATTGTGCTGTCTCTCCAGGACA
  CGTTGTGTGGCGTTGAAGAGCAGAAAGCAATGAAGTCCTTCTCCACGTGGGTCTTGTAAA
  CAGCATCTTCCTCCAGGTTCTCAGATGACTGTGAAGAGGCCACTTCCAAGGATGCTGGAG
  AGTCTCTGACCCACAGTTCCCCACGGTTTGCACCTCTGCAGGCCTGGACAATGATGACCT
  TGGGTTTGTCCTTCAGACTGAGGCAGTTGCGGTTGTTGAATATCTGGAAGATGGTGTCAT
  AAAGCAGCACATCTGGTTTTTTCTCATCATGCACAGTTCCGCAGATTCCCTCCAGGATGC
  CATGAGACATGAGTACCAAGAATGTGCTGTCAGAAGACTTGTGCTCTGGTCTGGTAGCAN
  ATGCCCTCAGCGCTGACTCCATATCCCTGGCTGTCAGATTCTCTTCTACATCTACACTAT
  AGTCCAGACCCCTCAGTAGCTCCTTCATCCCTGTGATGTCAAAGTCAGCTCCATTCCTCG
  NAGGCAGATGGTCAAACTCTGTATTGCATATGATGAGAAGCCAGNCGTGTGCCGGGTGGT
  TCTCTCCTTTATGGATAAGACTCTTCAGCTCTTTCTTTACATANNCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001225
• Insert Size: 1290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001225.3, NP_001216.1
• RefSeq Size: 1319 bp
• RefSeq ORF: 1134 bp
• Locus ID: 837
• UniProt ID: P49662
• Cytogenetics: 11q22.3
• Domains: CARD, CASc, ICE_p10, ICE_p20
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (alpha) encodes the longest isoform (alpha).