PBR (TSPO) (NM_000714) Human Untagged Clone

CAT#: SC111597

TSPO (untagged)-Human translocator protein (18kDa) (TSPO), transcript variant PBR

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PBR
• Synonyms: BPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111597 sequence for NM_000714 edited (data generated by NextGen Sequencing)
  ATGGCCCCGCCCTGGGTGCCCGCCATGGGCTTCACGCTGGCGCCCAGCCTGGGGTGCTTC
  GTGGGCTCCCGCTTTGTCCACGGCGAGGGTCTCCGCTGGTACGCCGGCCTGCAGAAGCCC
  TCGTGGCACCCGCCCCACTGGGTGCTGGGCCCTGTCTGGGGCACGCTCTACTCAGCCATG
  GGGTACGGCTCCTACCTGGTCTGGAAAGAGCTGGGAGGCTTCACAGAGAAGGCTGTGGTT
  CCCCTGGGCCTCTACACTGGGCAGCTGGCCCTGAACTGGGCATGGCCCCCCATCTTCTTT
  GGTGCCCGACAAATGGGCTGGGCCTTGGTGGATCTCCTGCTGGTCAGTGGGGCGGCGGCA
  GCCACTACCGTGGCCTGGTACCAGGTGAGCCCGCTGGCCGCCCGCCTGCTCTACCCCTAC
  CTGGCCTGGCTGGCCTTCGCGACCACACTCAACTACTGCGTATGGCGGGACAACCATGGC
  TGGCGTGGGGGACGGCGGCTGCCAGAGTGA
  
  Clone variation with respect to NM_000714.4
  439 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000714 unedited
  CACGAGGGAGCGTGCCCTCGCCGCTGAGCTCCCCTNGACAGCAGCTGCAGCAGCCATGGC
  CCCGCCCTGGGTGCCCGCCATGGGCTTCACGCTGGCGCCCAGCCTGGGGTGCTTCGTGGG
  CTCCCGCTTTGTCCACGGCGAGGGTCTCCGCTGGTACGCCGGCCTGCAGAAGCCCTCGTG
  GCACCCGCCCCACTGGGTGCTGGGCCCTGTCTGGGGCACGCTCTACTCAGCCATGGGGTA
  CGGCTCCTACCTGGTCTGGAAAGAGCTGGGAGGCTTCACAGAGAAGGCTGTGGTTCCCCT
  GGGCCTCTACACTGGGCAGCTGGCCCTGAACTGGGCATGGCCCCCCATCTTCTTTGGTGC
  CCGACAAATGGGCTGGGCCTTGGTGGATCTCCTGCTGGTCAGTGGGGCGGCGGCAGCCAC
  TACCGTGGCCTGGTACCAGGTGAGCCCGCTGGCCGCCCGCCTGCTCTACCCCTACCTGGC
  CTGGCTGGCCTTCGCGACCACACTCAACTACTGCGTATGGCGGGACAACCATGGCTGGCG
  TGGGGGACGGCGGCTGCCAGAGTGAGTGCCCGGCCCACCAGGGACTGCAGCTGCACCAGC
  AGGTGCCATCACGCTTGNTGATGTGGTGGCCGTCACGCTTTCATGACCACTGGGCCTGCT
  AGTCTGTCAGGGCCTTGGCCCCAGGGTCAGCAGAGCTTCAGAGGTGGCCCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000714 unedited
  TGATCAAAACTTTCTTCATCTTATAAAATTCCAGTTCCAGTACATGCTCTAAGCATGCAT
  AAAGCACAGGACACTGCTCCCGGGTGGGGGCTCATGTGGGGCCACCTCTGAAGCTCTGCT
  GACCCCTGGGCCAAGGCCCTGACAGACTATCAGGCCCAGTGGTCATGAAAGCGTGACGGC
  CACCACATCACAAGCGTGATGGCACCTGCTGGTGCAGCTGCAGTCCCTGGTGGGCCGGGC
  ACTCACTCTGGCAGCCGCCGTCCCCCACGCCATCCATGGTTGTCCCGCCATACGCAGTAG
  TTGAGTGTGGTCGCGAAGGCCAGCCAGGCCAGGTAGGGGTAGAGCAGGCGGGCGGCCAGC
  GGGCTCACCTGGTACCAGGCCACGGTAGTGGCTGCCGCCGCCCCACTGACCAGCATGAGA
  TCCACCAAGGCCCAGCCCATTTGTCGGGCACCAAAGAATATGGGGGGCCATGCCCAGTTC
  AGGGCCAGCTGCCCAGTGTAGAGGCCCAGGGGAACCACAGGCTTCTCTGTGAAGCCTCCC
  AGCTCTTTCCAGACCATGTAGGAGCCGTACCCCATGGCTGAGTAGATCGTGCCCCATACA
  GGGCCCATCACCCATTGGGGCCGGGTGCCACGATGGCCTTTTGTAAGCCGGCGTTCCAGC
  GGATGACCCCTCGCCGTGGACAAAGCGGGAGTCCACGAAGCACCCCAAGCTGGGCGCCAG
  CGTGAATCCATGGCGGGCACCCATGGCGGGGCCATGGCTGCAGCACCTGCTGTCACGGGA
  ACTTATCGGGGAAGGCCCTCTCCCCTCTGTGCCAATTCGCTGGCCGCCTTATAGTGAGCC
  CTCTTACAAAATCTGGTGGCTACCTAAAGAGCTCTGCTTTTTTAAACCCCCACGCTCAAC
  CCTACCGCCCATTGCCGTTAAGGGGGGGGGTTTTACAATTTTTGGAAGTCCCCTGACTCG
  GAGCCAAAATACTTCTTGACGCACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000714
• Insert Size: 890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000714.4, NP_000705.2
• RefSeq Size: 876 bp
• RefSeq ORF: 510 bp
• Locus ID: 706
• UniProt ID: P30536
• Cytogenetics: 22q13.2
• Domains: TspO_MBR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (PBR) consists of four exons and encodes the protein PBR.