PBR (TSPO) (NM_000714) Human Untagged Clone

SKU SC111597

TSPO (untagged)-Human translocator protein (18kDa) (TSPO), transcript variant PBR

Product Data

• Type: Human Untagged Clone
• Target Symbol: PBR
• Synonyms: BPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111597 sequence for NM_000714 edited (data generated by NextGen Sequencing); ATGGCCCCGCCCTGGGTGCCCGCCATGGGCTTCACGCTGGCGCCCAGCCTGGGGTGCTTC; GTGGGCTCCCGCTTTGTCCACGGCGAGGGTCTCCGCTGGTACGCCGGCCTGCAGAAGCCC; TCGTGGCACCCGCCCCACTGGGTGCTGGGCCCTGTCTGGGGCACGCTCTACTCAGCCATG; GGGTACGGCTCCTACCTGGTCTGGAAAGAGCTGGGAGGCTTCACAGAGAAGGCTGTGGTT; CCCCTGGGCCTCTACACTGGGCAGCTGGCCCTGAACTGGGCATGGCCCCCCATCTTCTTT; GGTGCCCGACAAATGGGCTGGGCCTTGGTGGATCTCCTGCTGGTCAGTGGGGCGGCGGCA; GCCACTACCGTGGCCTGGTACCAGGTGAGCCCGCTGGCCGCCCGCCTGCTCTACCCCTAC; CTGGCCTGGCTGGCCTTCGCGACCACACTCAACTACTGCGTATGGCGGGACAACCATGGC; TGGCGTGGGGGACGGCGGCTGCCAGAGTGA; ; Clone variation with respect to NM_000714.4; 439 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000714 unedited; CACGAGGGAGCGTGCCCTCGCCGCTGAGCTCCCCTNGACAGCAGCTGCAGCAGCCATGGC; CCCGCCCTGGGTGCCCGCCATGGGCTTCACGCTGGCGCCCAGCCTGGGGTGCTTCGTGGG; CTCCCGCTTTGTCCACGGCGAGGGTCTCCGCTGGTACGCCGGCCTGCAGAAGCCCTCGTG; GCACCCGCCCCACTGGGTGCTGGGCCCTGTCTGGGGCACGCTCTACTCAGCCATGGGGTA; CGGCTCCTACCTGGTCTGGAAAGAGCTGGGAGGCTTCACAGAGAAGGCTGTGGTTCCCCT; GGGCCTCTACACTGGGCAGCTGGCCCTGAACTGGGCATGGCCCCCCATCTTCTTTGGTGC; CCGACAAATGGGCTGGGCCTTGGTGGATCTCCTGCTGGTCAGTGGGGCGGCGGCAGCCAC; TACCGTGGCCTGGTACCAGGTGAGCCCGCTGGCCGCCCGCCTGCTCTACCCCTACCTGGC; CTGGCTGGCCTTCGCGACCACACTCAACTACTGCGTATGGCGGGACAACCATGGCTGGCG; TGGGGGACGGCGGCTGCCAGAGTGAGTGCCCGGCCCACCAGGGACTGCAGCTGCACCAGC; AGGTGCCATCACGCTTGNTGATGTGGTGGCCGTCACGCTTTCATGACCACTGGGCCTGCT; AGTCTGTCAGGGCCTTGGCCCCAGGGTCAGCAGAGCTTCAGAGGTGGCCCCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000714 unedited; TGATCAAAACTTTCTTCATCTTATAAAATTCCAGTTCCAGTACATGCTCTAAGCATGCAT; AAAGCACAGGACACTGCTCCCGGGTGGGGGCTCATGTGGGGCCACCTCTGAAGCTCTGCT; GACCCCTGGGCCAAGGCCCTGACAGACTATCAGGCCCAGTGGTCATGAAAGCGTGACGGC; CACCACATCACAAGCGTGATGGCACCTGCTGGTGCAGCTGCAGTCCCTGGTGGGCCGGGC; ACTCACTCTGGCAGCCGCCGTCCCCCACGCCATCCATGGTTGTCCCGCCATACGCAGTAG; TTGAGTGTGGTCGCGAAGGCCAGCCAGGCCAGGTAGGGGTAGAGCAGGCGGGCGGCCAGC; GGGCTCACCTGGTACCAGGCCACGGTAGTGGCTGCCGCCGCCCCACTGACCAGCATGAGA; TCCACCAAGGCCCAGCCCATTTGTCGGGCACCAAAGAATATGGGGGGCCATGCCCAGTTC; AGGGCCAGCTGCCCAGTGTAGAGGCCCAGGGGAACCACAGGCTTCTCTGTGAAGCCTCCC; AGCTCTTTCCAGACCATGTAGGAGCCGTACCCCATGGCTGAGTAGATCGTGCCCCATACA; GGGCCCATCACCCATTGGGGCCGGGTGCCACGATGGCCTTTTGTAAGCCGGCGTTCCAGC; GGATGACCCCTCGCCGTGGACAAAGCGGGAGTCCACGAAGCACCCCAAGCTGGGCGCCAG; CGTGAATCCATGGCGGGCACCCATGGCGGGGCCATGGCTGCAGCACCTGCTGTCACGGGA; ACTTATCGGGGAAGGCCCTCTCCCCTCTGTGCCAATTCGCTGGCCGCCTTATAGTGAGCC; CTCTTACAAAATCTGGTGGCTACCTAAAGAGCTCTGCTTTTTTAAACCCCCACGCTCAAC; CCTACCGCCCATTGCCGTTAAGGGGGGGGGTTTTACAATTTTTGGAAGTCCCCTGACTCG; GAGCCAAAATACTTCTTGACGCACG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000714
• Insert Size: 890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000714.4, NP_000705.2
• RefSeq Size: 876 bp
• RefSeq ORF: 510 bp
• Locus ID: 706
• UniProt ID: P30536
• Cytogenetics: 22q13.2
• Domains: TspO_MBR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Summary: Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. [provided by RefSeq, Feb 2012]; Transcript Variant: This variant (PBR) consists of four exons and encodes the protein PBR.