FE65 (APBB1) (NM_001164) Human Untagged Clone

CAT#: SC111588

APBB1 (untagged)-Human amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) (APBB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FE65
• Synonyms: FE65; MGC:9072; RIR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGTTCCATCATCACTGAGCCAGTCGGCCATTAATGCCAACAGCCACGGAGGCCCCGCACTGAGCC
  TACCCCTGCCTCTGCACGCTGCCCACAACCAGCTGCTCAACGCCAAGCTGCAGGCCACAGCTGTGGGACC
  CAAGGACCTGCGCAGCGCCATGGGGGAGGGTGGTGGGCCTGAGCCAGGCCCTGCCAATGCCAAGTGGCTA
  AAAGAGGGCCAGAACCAGCTCCGGCGGGCCGCCACGGCCCACCGTGACCAGAATCGCAATGTGACCTTGA
  CCTTGGCGGAGGAGGCCAGCCAGGAGCCTGAGATGGCACCCTTGGGCCCCAAAGGCCTGATACACCTGTA
  CTCTGAGCTGGAGCTCTCAGCTCACAACGCAGCCAACCGAGGCCTACGAGGACCTGGCCTGATCATCAGC
  ACTCAAGAGCAGGGGCCAGATGAGGGAGAGGAGAAGGCGGCCGGGGAGGCCGAGGAGGAGGAGGAGGATG
  ATGATGATGAAGAGGAGGAGGAGGACTTATCTTCTCCCCCAGGGCTGCCTGAGCCCCTGGAGAGTGTGGA
  GGCCCCTCCCAGGCCCCAAGCCCTTACAGATGGCCCCCGGGAACACAGCAAGAGTGCCAGCCTCCTGTTT
  GGCATGCGGAACAGTGCAGCCAGTGATGAGGACTCAAGCTGGGCTACCTTATCCCAGGGCAGCCCCTCCT
  ATGGCTCCCCAGAGGACACAGATTCCTTCTGGAACCCCAACGCCTTCGAGACGGATTCCGACCTGCCGGC
  TGGATGGATGAGGGTCCAGGACACCTCAGGGACCTATTACTGGCACATCCCAACAGGGACCACCCAGTGG
  GAACCCCCCGGCCGGGCCTCCCCCTCACAGGGGAGCAGCCCCCAAGAGGAGTCCCAGCTCACCTGGACAG
  GTTTTGCTCATGGAGAAGGCTTTGAGGATGGAGAATTTTGGAAGGATGAACCCAGTGATGAGGCCCCAAT
  GGAGCTGGGACTGAAGGAACCTGAGGAGGGGACGTTGACCTTCCCAGCTCAGAGCCTCAGCCCAGAGCCG
  TTGCCCCAAGAGGAGGAGAAGCTTCCCCCACGGAATACCAACCCAGGGATCAAGTGTTTCGCCGTGCGCT
  CCCTAGGCTGGGTAGAGATGACCGAGGAGGAGCTGGCCCCTGGACGCAGCAGTGTGGCAGTCAACAATTG
  CATCCGTCAGCTCTCTTACCACAAAAACAACCTGCATGACCCCATGTCTGGGGGCTGGGGGGAAGGAAAG
  GATCTGCTACTGCAGCTGGAGGATGAGACACTAAAGCTAGTGGAGCCACAGAGCCAGGCACTGCTGCACG
  CCCAACCCATCATCAGCATCCGCGTGTGGGGCGTCGGGCGGGACAGTGGAAGAGAGAGGGACTTTGCCTA
  CGTAGCTCGTGATAAGCTGACCCAGATGCTCAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGAAC
  ATCGCCACCAGCCTGCATGAGATCTGCTCTAAGATCATGGCCGAACGGCGTAATGCCCGCTGCTTGGTAA
  ATGGACTCTCCCTGGACCACTCTAAACTTGTGGATGTCCCTTTCCAAGTGGAATTCCCAGCGCCTAAGAA
  TGAGTTGGTCCAGAAGTTCCAAGTCTATTACCTGGGGAATGTACCTGTTGCTAAACCTGTTGGGGTAGAT
  GTGATTAATGGGGCCCTCGAGTCAGTCCTGTCCTCCAGCAGCCGTGAACAATGGACCCCAAGTCATGTCA
  GTGTGGCCCCTGCTACCCTCACCATCTTGCACCAGCAGACAGAGGCAGTGCTGGGAGAGTGTCGGGTGCG
  TTTCCTCTCCTTCCTGGCCGTGGGCAGAGATGTCCACACGTTTGCATTCATCATGGCTGCCGGCCCAGCC
  TCCTTCTGCTGCCACATGTTCTGGTGCGAGCCCAATGCTGCCAGCCTCTCAGAGGCTGTGCAGGCTGCGT
  GCATGCTTCGCTACCAGAAGTGTCTGGATGCCCGTTCCCAGGCCTCCACCTCCTGCCTCCCAGCACCCCC
  TGCTGAGTCTGTGGCACGGCGTGTAGGGTGGACTGTCCGCAGGGGTGTTCAGTCGCTGTGGGGCTCCCTG
  AAGCCCAAACGGCTGGGGGCCCATACCCCATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001164 unedited
  TTCAAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAGCCCG
  AACCCCGCAGCCTGAGCCACCTCCGTCATCTGGGCCCGGGGCCTCACCGCGCAGGAGCTG
  CCAAGGCCATGTCTGTTCCATCATCACTGAGCCAGTCGGCCATTAATGCCAACAGCCACG
  GAGGCCCCGCACTGAGCCTACCCCTGCCTCTGCACGCTGCCCACAACCAGCTGCTCAACG
  CCAAGCTGCAGGCCACAGCTGTGGGACCCAAGGACCTGCGCAGCGCCATGGGGGAGGGTG
  GTGGGCCTGAGCCAGGCCCTGCCAATGCCAAGTGGCTAAAAGAGGGCCAGAACCAGCTCC
  GGCGGGCCGCCACGGCCCACCGTGACCAGAATCGCAATGTGACCTTGACCTTGGCGGAGG
  AGGCCAGCCAGGAGCCTGAGATGGCACCCTTGGGCCCCAAAGGCCTGATACACCTGTACT
  CTGAGCTGGAGCTCTCAGCTCACAACGCAGCCAACCGAGGCCTACGANGACCTGGCCTGA
  TCATCAGCACTCAAGAGCAGGGGCCANATGANGGAGAAGAGAAAGCGGCCGGNGAAGCCC
  ANNGAGANGANGANGATGATGATGATGAAAAAGATNGAGGAGACTTATCTTTCTTCCCCA
  GGGCTGCCCTGAGCCCCTGGAGAGTGTGGAAGGCCCCTCCCAAGCCCCCAAGCCCTTTAC
  AGAGGCCCCCCGGGAACCACAAGCAGAAGGCCAGCCCTCCTGTTTGGCATTGCGGAAAGT
  GCACCCAGTGATGAAGACTTAACCTTGGCTACCTTATCCAAGGCAAGCCCTCTATGGTTC
  CCAGAAGAAACAAAATCCTTTGGAACCCCACGCCTTCAAAACGTATCCAACCTGCGCCTT
  TATGATAAAAGGTCCAACACACCTTAGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001164 unedited
  GTACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGCAGTGCAGACAG
  ACTTTATTAGTGATATCAATACAGCAGAGGTGCCCATGGAGCAGGGGGAAGGGACCCATG
  CCTGGACCAGTCCCTTCCTCCTTCTGCCCCTGCTGGGTCCAGGAGGATGAGGCCTGGCCT
  GGACCAGTTCCTCCTGTTCCACCTGAAACACATGGGGATGGAGAACCAGGGCTGGCCTTG
  CTTCCTGCTCCTCTTGTTACCACTCCAGTGTTATCACTTCCTTGAAGGGATTAGATCTCT
  CCCTCCCCAGCTCCTAGGCAGGGAACCGTAGCTACTGGGGAGGGGCATATTTGGGAGGCC
  TGAGGCCTAGGAATAGCCTCTAGACCCCTCCCTGACCCACACCCTTTAGTTCCCTGGGGC
  CCAACACAAGCAGGTGGAGGGAAGGTGGGGGCTTCTTCATGGGGTATGGGCCCCCAGCCG
  TTTGGGCTTCAGGGAGCCCCACAGCGACTGAACACCCCTGCGGACAGTCCACCCTACACG
  CCGTGCCACAGACTCAGCAGGGGGTGCTGGGAGGCAGGAGGTGGAGGCCTGGGAACGGGC
  ATCCAGACACTTCTGGTAGCGAAGCATGCACGCAGCCTGCACAGCCTCTGAGAGGCTGGC
  AGCATTGGGCTCGCACCAGAACATGTGGCAGCAGAAGGAGGCTGGGCCGGCAGCCATGAT
  GAATGCAAACGTGTGGACATCTCTGCCCACGCCAGNAAGGAGAGGAAACGCACCCGACAC
  TCTCCCAGCACTGGCTCTGTCTGCTGGTGCAAGATGGTGAGGGTAGCAGGGGCCACACTG
  ACATGACTTGGNGTCCATTGTTCACGGCTGCTGGAGACCAGACTGACTCNAGGGCCCCAT
  TATCACATCTACCCAACAGGTTTAGCACAGGTACATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001164
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164.2, NP_001155.1
• RefSeq Size: 2653 bp
• RefSeq ORF: 2133 bp
• Locus ID: 322
• UniProt ID: O00213
• Cytogenetics: 11p15.4
• Domains: WW, PID
• Protein Families: Transcription Factors
• Protein Pathways: Alzheimer's disease
• Gene Summary: The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a, also known as E9). This isoform is exclusively expressed in neurons.