RAMP1 (NM_005855) Human Untagged Clone

CAT#: SC111143

RAMP1 (untagged)-Human receptor (G protein-coupled) activity modifying protein 1 (RAMP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAMP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005855 edited
  ATGGCCCGGGCCCTGTGCCGCCTCCCGCGGCGCGGCCTCTGGCTGCTCCTGGCCCATCAC
  CTCTTCATGACCACTGCCTGCCAGGAGGCTAACTACGGTGCCCTCCTCCGGGAGCTCTGC
  CTCACCCAGTTCCAGGTAGACATGGAGGCCGTCGGGGAGACGCTGTGGTGTGACTGGGGC
  AGGACCATCAGGAGCTACAGGGAGCTGGCCGACTGCACCTGGCACATGGCGGAGAAGCTG
  GGCTGCTTCTGGCCCAATGCAGAGGTGGACAGGTTCTTCCTGGCAGTGCATGGCCGCTAC
  TTCAGGAGCTGCCCCATCTCAGGCAGGGCCGTGCGGGACCCGCCCGGCAGCATCCTCTAC
  CCCTTCATCGTGGTCCCCATCACGGTGACCCTGCTGGTGACGGCACTGGTGGTCTGGCAG
  AGCAAGCGCACTGAGGGCATTGTGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005855 unedited
  NGGGTCGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCCACGAGGGCGCGTG
  GCGAGCGGACTCGACTCGGCACCGCTGTGCACCATGGCCCGGGCCCTGTGCCGCCTCCCG
  CGGCGCGGCCTCTGGCTGCTCCTGGCCCATCACCTCTTCATGACCACTGCCTGCCAGGAG
  GCTAACTACGGTGCCCTCCTCCGGGAGCTCTGCCTCACCCAGTTCCAGGTAGACATGGAG
  GCCGTCGGGGAGACGCTGTGGTGTGACTGGGGCAGGACCATCAGGAGCTACAGGGAGCTG
  GCCGACTGCACCTGGCACATGGCGGAGAAGCTGGGCTGCTTCTGGCCCAATGCAGAGGTG
  GACAGGTTCTTCCTGGCAGTGCATGGCCGCTACTTCAGGAGCTGCCCCATCTCAGGCAGG
  GCCGTGCGGGACCCGCCCGGCAGCATCCTCTACCCCTTCATCGTGGTCCCCATCACGGTG
  ACCCTGCTGGTGACGGCACTGGTGGTCTGGCAGAGCAAGCGCACTGAGGGCATTGTGTAG
  GCGGGGCCCANGCTGCCCGCGGGTGCACCCAGGCTGCAGGGTGAGGCCAGGCAGGCCTGG
  GTAGGGGCAGCTTCTGGAGCCTTGNGACAGAGCANGCCCACAATGCCCCCCTTCTTCCAG
  CCAAGAAGAGCTCACAGGAGTCCAGAGTAGCCGAGGCTCTGGTATTAACCTGGAAGCCCC
  CCTGGCTGGAGGCCACCGCCACCCTAGGAAGGNGGCAGGGGACGTGACCTTGACTTACCT
  CTGGNAAGGGTCCCAGCCTAGACTGCTTACCCCATAGCCACATTTGTGGATGAGTGGTTT
  GTGATTAAAAGGNATGNNTCTGAAAAAAAAAAAAAAAAAAACTCGACTCTAGTTGCGGCC
  GNCGTCATAGCTGTTTCCTGACAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005855 unedited
  TGTACGCGGCCGCAATCTATATCGAGTTTTTTTTTTTTTTTTTTTTTTCAAAAACATCCC
  TTTTAATCACAAACCACTCATCCACAAATGTGGCTATGGGGTAAGCAGTCTAGGCTGGGA
  CCCTTTCCAAAGGTAAGTCAAGGTCACGTCCCTGCCCCCTTCCTAGGGTGGCGGTGGCCT
  CCAGCCAGGGGGGCTTCCAGGTTAATACCAAAGCCTCGGCTACTCTGGACTCCTGTGAGC
  TCTTCTTGGCTGGAAAAAGGGGGGCATTGTGGGCCTGCTCTGTCCCAAGGCTCCAAAAGC
  TGCCCCTACCCAGGCCTGCCTGGCCTCACCCTGCAGCCTGGGTGCACCCGCGGGCAGCCT
  GGGCCCCGCCTACACAATGCCCTCATTGCGCTTGTTCTGCCAAACCACCATTGCCGTCAC
  CTGCAGGGTCACCGTGATGGGGACCACCACGAACGGCCTAAGGATGCTGCCGGTCCGTCT
  CCCCACGCCCCTCCTTGAAAAAGGGGAATTTGTTGAATCATCCCCCATCCCCGCCCTGCA
  CTACCCGCGTCCACCTCGAATGGCCCTCATACTCCCTTAAATTCTCTCTCATATCACCCC
  TCCCCCCTCATCTGTCCCCCACTCGCCCTCCCTTCTCCCCCTTTCCCTCTCTCCCGTCAC
  ACATGACTGTCCCCTATTACTCCACCCCTGCACCCCACTTCCCTCTCTTACTATCCTCTC
  TCCTTCTCGCCCCCCTCATTTTCGTCTTTATGTATTGCTTTCTTTCGTTCCCCCCTGCAC
  CTCTCCTCCCCCCCCCCACCTCTCGCTCCTCGTTTGTCTCACATCTATACTTGGATCTAT
  CTCCTTTTTTCCTATCCTCACCTTCTCTCTTCTCCCCCCCCCTTATATCTTCCATGCATC
  TTATTCCCACCATCTACTTTCACTATCTTATCCCTCCACCTCCATTACTCCACACCCACA
  TCAACTAACTACTCCATACTCTATTCACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005855
• Insert Size: 880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005855.2, NP_005846.1
• RefSeq Size: 922 bp
• RefSeq ORF: 447 bp
• Locus ID: 10267
• UniProt ID: O60894
• Cytogenetics: 2q37.3
• Domains: RAMP
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
  Transcript Variant: This variant (1) encodes the longer isoform (1).