RAMP1 (NM_005855) Human Untagged Clone

SKU
SC111143
RAMP1 (untagged)-Human receptor (G protein-coupled) activity modifying protein 1 (RAMP1)
$150.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RAMP1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_005855 edited
ATGGCCCGGGCCCTGTGCCGCCTCCCGCGGCGCGGCCTCTGGCTGCTCCTGGCCCATCAC
CTCTTCATGACCACTGCCTGCCAGGAGGCTAACTACGGTGCCCTCCTCCGGGAGCTCTGC
CTCACCCAGTTCCAGGTAGACATGGAGGCCGTCGGGGAGACGCTGTGGTGTGACTGGGGC
AGGACCATCAGGAGCTACAGGGAGCTGGCCGACTGCACCTGGCACATGGCGGAGAAGCTG
GGCTGCTTCTGGCCCAATGCAGAGGTGGACAGGTTCTTCCTGGCAGTGCATGGCCGCTAC
TTCAGGAGCTGCCCCATCTCAGGCAGGGCCGTGCGGGACCCGCCCGGCAGCATCCTCTAC
CCCTTCATCGTGGTCCCCATCACGGTGACCCTGCTGGTGACGGCACTGGTGGTCTGGCAG
AGCAAGCGCACTGAGGGCATTGTGTAG
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005855 unedited
NGGGTCGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCCACGAGGGCGCGTG
GCGAGCGGACTCGACTCGGCACCGCTGTGCACCATGGCCCGGGCCCTGTGCCGCCTCCCG
CGGCGCGGCCTCTGGCTGCTCCTGGCCCATCACCTCTTCATGACCACTGCCTGCCAGGAG
GCTAACTACGGTGCCCTCCTCCGGGAGCTCTGCCTCACCCAGTTCCAGGTAGACATGGAG
GCCGTCGGGGAGACGCTGTGGTGTGACTGGGGCAGGACCATCAGGAGCTACAGGGAGCTG
GCCGACTGCACCTGGCACATGGCGGAGAAGCTGGGCTGCTTCTGGCCCAATGCAGAGGTG
GACAGGTTCTTCCTGGCAGTGCATGGCCGCTACTTCAGGAGCTGCCCCATCTCAGGCAGG
GCCGTGCGGGACCCGCCCGGCAGCATCCTCTACCCCTTCATCGTGGTCCCCATCACGGTG
ACCCTGCTGGTGACGGCACTGGTGGTCTGGCAGAGCAAGCGCACTGAGGGCATTGTGTAG
GCGGGGCCCANGCTGCCCGCGGGTGCACCCAGGCTGCAGGGTGAGGCCAGGCAGGCCTGG
GTAGGGGCAGCTTCTGGAGCCTTGNGACAGAGCANGCCCACAATGCCCCCCTTCTTCCAG
CCAAGAAGAGCTCACAGGAGTCCAGAGTAGCCGAGGCTCTGGTATTAACCTGGAAGCCCC
CCTGGCTGGAGGCCACCGCCACCCTAGGAAGGNGGCAGGGGACGTGACCTTGACTTACCT
CTGGNAAGGGTCCCAGCCTAGACTGCTTACCCCATAGCCACATTTGTGGATGAGTGGTTT
GTGATTAAAAGGNATGNNTCTGAAAAAAAAAAAAAAAAAAACTCGACTCTAGTTGCGGCC
GNCGTCATAGCTGTTTCCTGACAAT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005855 unedited
TGTACGCGGCCGCAATCTATATCGAGTTTTTTTTTTTTTTTTTTTTTTCAAAAACATCCC
TTTTAATCACAAACCACTCATCCACAAATGTGGCTATGGGGTAAGCAGTCTAGGCTGGGA
CCCTTTCCAAAGGTAAGTCAAGGTCACGTCCCTGCCCCCTTCCTAGGGTGGCGGTGGCCT
CCAGCCAGGGGGGCTTCCAGGTTAATACCAAAGCCTCGGCTACTCTGGACTCCTGTGAGC
TCTTCTTGGCTGGAAAAAGGGGGGCATTGTGGGCCTGCTCTGTCCCAAGGCTCCAAAAGC
TGCCCCTACCCAGGCCTGCCTGGCCTCACCCTGCAGCCTGGGTGCACCCGCGGGCAGCCT
GGGCCCCGCCTACACAATGCCCTCATTGCGCTTGTTCTGCCAAACCACCATTGCCGTCAC
CTGCAGGGTCACCGTGATGGGGACCACCACGAACGGCCTAAGGATGCTGCCGGTCCGTCT
CCCCACGCCCCTCCTTGAAAAAGGGGAATTTGTTGAATCATCCCCCATCCCCGCCCTGCA
CTACCCGCGTCCACCTCGAATGGCCCTCATACTCCCTTAAATTCTCTCTCATATCACCCC
TCCCCCCTCATCTGTCCCCCACTCGCCCTCCCTTCTCCCCCTTTCCCTCTCTCCCGTCAC
ACATGACTGTCCCCTATTACTCCACCCCTGCACCCCACTTCCCTCTCTTACTATCCTCTC
TCCTTCTCGCCCCCCTCATTTTCGTCTTTATGTATTGCTTTCTTTCGTTCCCCCCTGCAC
CTCTCCTCCCCCCCCCCACCTCTCGCTCCTCGTTTGTCTCACATCTATACTTGGATCTAT
CTCCTTTTTTCCTATCCTCACCTTCTCTCTTCTCCCCCCCCCTTATATCTTCCATGCATC
TTATTCCCACCATCTACTTTCACTATCTTATCCCTCCACCTCCATTACTCCACACCCACA
TCAACTAACTACTCCATACTCTATTCACG
Restriction Sites NotI-NotI
ACCN NM_005855
Insert Size 447 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005855.2, NP_005846.1
RefSeq Size 922 bp
RefSeq ORF 447 bp
Locus ID 10267
UniProt ID O60894
Cytogenetics 2q37.3
Domains RAMP
Protein Families Druggable Genome, Transmembrane
Protein Pathways Vascular smooth muscle contraction
Summary The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Transcript Variant: This variant (1) encodes the longer isoform (1).
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.