ATP6IP2 (ATP6AP2) (NM_005765) Human Untagged Clone

CAT#: SC111138

ATP6AP2 (untagged)-Human ATPase, H+ transporting, lysosomal accessory protein 2 (ATP6AP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6IP2
• Synonyms: APT6M8-9; ATP6IP2; ATP6M8-9; CDG2R; ELDF10; HT028; M8-9; MRXE; MRXSH; MSTP009; PRR; RENR; XMRE; XPDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111138 sequence for NM_005765 edited (data generated by NextGen Sequencing)
  ATGGCTGTGTTTGTCGTGCTCCTGGCGTTGGTGGCGGGTGTTTTGGGGAACGAGTTTAGT
  ATATTAAAATCACCAGGGTCTGTTGTTTTCCGAAATGGAAATTGGCCTATACCAGGAGAG
  CGGATCCCAGACGTGGCTGCATTGTCCATGGGCTTCTCTGTGAAAGAAGACCTTTCTTGG
  CCAGGACTCGCAGTGGGTAACCTGTTTCATCGTCCTCGGGCTACCGTCATGGTGATGGTG
  AAGGGAGTGAACAAACTGGCTCTACCCCCAGGCAGTGTCATTTCGTACCCTTTGGAGAAT
  GCAGTTCCTTTTAGTCTTGACAGTGTTGCAAATTCCATTCACTCCTTATTTTCTGAGGAA
  ACTCCTGTTGTTTTGCAGTTGGCTCCCAGTGAGGAAAGAGTGTATATGGTAGGGAAGGCA
  AACTCAGTGTTTGAAGACCTTTCAGTCACCTTGCGCCAGCTCCGTAATCGCCTGTTTCAA
  GAAAACTCTGTTCTCAGTTCACTCCCCCTCAATTCTCTGAGTAGGAACAATGAAGTTGAC
  CTGCTCTTTCTTTCTGAACTGCAAGTGCTACATGATATTTCAAGCTTGCTGTCTCGTCAT
  AAGCATCTAGCCAAGGATCATTCTCCTGATTTATATTCACTGGAGCTGGCAGGTTTGGAT
  GAAATTGGGAAGCGTTATGGGGAAGACTCTGAACAATTCAGAGATGCTTCTAAGATCCTT
  GTTGACGCTCTGCAAAAGTTTGCAGATGACATGTACAGTCTTTATGGTGGGAATGCAGTG
  GTAGAGTTAGTCACTGTCAAGTCATTTGACACCTCCCTCATTAGGAAGACAAGGACTATC
  CTTGAGGCAAAACAAGCGAAGAACCCAGCAAGTCCCTATAACCTTGCATATAAGTATAAT
  TTTGAATATTCCGTGGTTTTCAACATGGTACTTTGGATAATGATCGCCTTGGCCTTGGCT
  GTGATTATCACCTCTTACAATATTTGGAACATGGATCCTGGATATGATAGCATCATTTAT
  AGGATGACAAACCAGAAGATTCGAATGGATTGA
  
  Clone variation with respect to NM_005765.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005765 unedited
  GTATATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGCCCGTT
  CCGTGTCGCCCCGCAGTGCTGCGGCCGCCGCGGCACCATGGCTGTGTTTGTCGTGCTCCT
  GGCGTTGGTGGCGGGTGTTTTGGGGAACGAGTTTAGTATATTAAAATCACCAGGGTCTGT
  TGTTTTCCGAAATGGAAATTGGCCTATACCAGGAGAGCGGATCCCAGACGTGGCTGCATT
  GTCCATGGGCTTCTCTGTGAAAGAAGACCTTTCTTGGCCAGGACTCGCAGTGGGTAACCT
  GTTTCATCGTCCTCGGGCTACCGTCATGGTGATGGTGAAGGGAGTGAACAAACTGGCTCT
  ACCCCCAGGCAGTGTCATTTCGTACCCTTTGGAGAATGCAGTTCCTTTTAGTCTTGACAG
  TGTTGCAAATTCCATTCACTCCTTATTTTCTGAGGAAACTCCTGTTGTTTTGCAGTTGGC
  TCCCAGTGAGGAAAGAGTGTATATGGTAGGGAAGGCAAACTCAGTGTTTGAAGACCTTTC
  AGTCACCTTGCGCCAGCTCCGTAATCGCCTGTTTCAAGAAAACTCTGTTCTCAGTTCACT
  CCCCCTCAATTCTCTGAGTAGGAACAATGAAGTTGACCTGCTCTTTCTTTCTGAACTGCA
  AGTGCTACATGATATTTCAAGCTTGCTGTCTCGTCATAAGCATCTNAGCCAAGATCATTC
  TCCTGATTTATATTCACTGGAGCTGGCANGTTTGGATGAAATTGGGAAGCGTTATGGGGA
  AGACTCTGAACCATTCAGAGATGCTTCTAAGATCCTTGTTGACGCTCTGCAAAGTTTGCA
  GATGACATGTACAGTCCTTTATGGTGGGAATGCCATGGTAGAGTTATCACTGTCCAGTCA
  TTTGACACTCCCTCATTAGGAGACAGGACATNCTTTGTAGCAACAACGAGGACCCGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005765 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGGAACATCAT
  TAACTTTATTTGTCACTCTTGATAGACATTGGTCCACTCCAACATAAAAAGTAGAATTCA
  CCCACTTCCACTTAATATTCTATAGAATGAAGTTGTACCACAAACCATAGTAACTTACAC
  AGGGGGGAAAAGTTACTGAAATAAAAATGTTTTATATTCATGCCTCAAAATGTCCCTCAG
  GGATTTTTGTGTGTATAATGAAAAGCATCTATATACTCCACAAGGCTAGTTTCTTCTTAG
  ATCAATGTAGTATTTTTAGTATCCATATTAGCCCATGTAGGTCAACAAACTTGCAGAAGA
  GGGTTAAAAAACAGCTGTCCTCTGTTACTCAGATACAGTTCCAAAACTAAGCGATTATAT
  AAGCACATCCATATTTTAGGGCTACTCTAAGTTAAAAACCTTTTCTCTTGTTTCAGAGTT
  ATTTACATCAAATTAAGACATTTACAAATTGTTCATAGTATACAATAGCCCAAATATGAT
  TTTCACCTATGCTGTGTAAAGAAGTTAAGCATTCGTAAGTTTGTCTAATAAATTCAGTGC
  ACTTTTTTCCATAACACGAGCTATTCTAAATGTTTTACATTTCTTTCAGTGCATATTTCC
  AAATTCATTAAACAGAATGAAATCAATGTTATTAAATGGCTATATCATAATATTCAAGCA
  TATTATGGAATCTATACCACAGTGGGATTCACGTCAATACTATAATTCACTCTAGAAAAC
  ATCACAGGCACCACAAAATAAAGAACCAAATTTGATTTTTTTTTATNNAATGTAAGTATA
  CTATCTACTTTTAAGCACACTANAAGATATATTTTAACANACAGCCAATTTCCAACCCCT
  TTTCTAATNCTGCACAGGTACATTCATTCATTCGAATCTCTGGNTTGCATCCTATAATGA
  TGCNTNATATCCAGNATCCATGTCCAAATATGTAGAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005765
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005765.2, NP_005756.2
• RefSeq Size: 2044 bp
• RefSeq ORF: 1053 bp
• Locus ID: 10159
• UniProt ID: O75787
• Cytogenetics: Xp11.4
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq, Jul 2008]