N WASP (WASL) (NM_003941) Human Untagged Clone

SKU SC111092

WASL (untagged)-Human Wiskott-Aldrich syndrome-like (WASL)

Product Data

• Type: Human Untagged Clone
• Target Symbol: N WASP
• Synonyms: N-WASP; NWASP; WASPB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111092 sequence for NM_003941 edited (data generated by NextGen Sequencing); ATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTGGGGTCTCTG; TTGCTCACCCCGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAATGTGTGACT; ATGTCTTCAGCAGTGGTGCAGTTATATGCAGCAGATCGGAACTGTATGTGGTCAAAGAAG; TGCAGTGGTGTTGCTTGTCTTGTTAAGGACAATCCACAGAGATCTTATTTTTTAAGAATA; TTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGCTATACAATAACTTTGTATAT; AATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTTGCTCTTAAT; TTTGCCAATGAAGAAGAAGCAAAAAAATTTCGAAAAGCAGTTACAGACCTTTTGGGCCGT; CGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTACCCATGGCT; ACAGTTGATATAAAAAATCCAGAAATCACAACAAATAGATTTTATGGTCCACAAGTCAAC; AACATCTCCCATACCAAAGAAAAGAAGAAGGGAAAAGCTAAAAAGAAGAGATTAACCAAG; GCAGATATAGGAACACCAAGCAATTTCCAGCACATTGGACATGTTGGTTGGGATCCAAAT; ACAGGCTTTGATCTGAATAATTTGGATCCAGAATTGAAGAATCTTTTCGATATGTGTGGA; ATCTCAGAGGCACAACTTAAAGACAGAGAAACATCAAAAGTTATATATGACTTTATTGAA; AAAACAGGAGGTGTTGAAGCTGTTAAAAATGAACTGCGGAGGCAAGCACCACCACCTCCA; CCACCATCAAGGGGAGGGCCACCTCCTCCTCCTCCCCCTCCACACAACTCAGGTCCTCCT; CCTCCTCCTGCTAGGGGAAGAGGCGCTCCTCCCCCACCACCTTCAAGAGCTCCCACAGCT; GCACCTCCACCACCGCCTCCTTCCAGGCCAAGTGTAGCAGTCCCTCCACCACCGCCAAAT; AGGATGTACCCTCCTCCACCTCCAGCCCTTCCCTCCTCAGCACCTTCAGGGCCTCCACCA; CCACCTCCATCTGTGTTGGGGGTAGGGCCAGTGGCACCACCCCCACCGCCTCCACCTCCA; CCTCCTCCTGGGCCACCGCCCCCGCCTGGCCTGCCTTCTGATGGGGACCATCAGGTTCCA; ACTACTGCAGGAAACAAAGCAGCTCTTTTAGATCAAATTAGAGAGGGTGCTCAGCTAAAA; AAAGTGGAGCAGAACAGTCGGCCAGTGTCCTGCTCTGGACGAGATGCACTGTTAGACCAG; ATACGACAGGGTATCCAACTAAAATCTGTGGCTGATGGCCAAGAGTCTACACCACCAACA; CCTGCACCCACTTCAGGAATTGTGGGTGCATTAATGGAAGTGATGCAGAAAAGGAGCAAA; GCCATTCATTCTTCAGATGAAGATGAAGATGAAGATGATGAAGAAGATTTTGAGGATGAT; GATGAGTGGGAAGACTGA; ; Clone variation with respect to NM_003941.2; 57 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003941 unedited; GGAAGCGTACAAATTTGTAAACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGCC; GCCGCTGCGGGAGGGTTGGGGGAGGAGTTGGGAGTTTAGCGCAGTCGCCGGAGTGCGAGG; ACAACGACCATCCGGCCCTAGCCTGGCCGGGCGGGTGCCGGGAGCTTCCCTTTCTCAGCG; CGGCGCGAAGGTGGCTCGCCGTCAGCGCCTGCTTCCCTCGACCTCGTCCTCCTCCCCGCT; CCGGAGGAGCTGCGAGATGTGGCGCCTCTGACTCCACTTCTCCCCGCCCCTGTCACCGAG; AGGGGGAACGAGCTCTCGCCCACTCGCCGGAGGAGACGGCCCTGGACTCCCAACCCCGCC; GGCGAAACCATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTG; GGGTCTCTGTTGCTCACCCCGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAA; TGTGTGACTATGTCTTCAGCAGTGGTGCAGTTATATGCAGCAGATCGGAACTGTATGTGG; TCAAAGAAGTGCAGTGGTGTTGCTTGTCTTGTTAAGGACAATCCACAGAGATCTTATTTT; TTAAGAATATTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGCTATACAATAAC; TTTGTATATAATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTT; GCTCTTAATTTTGCCAATGAAGAAGAAGCANAAAAATTTCGAAAAGCAGTTACAGACCTT; TTGGGCCGTCGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTA; CCATGGCTACAGTTGATATAAAAAATCCAGAAATCACACANATAGATTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003941 unedited; CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGGTCAAATTAATTTTATT; ATGCTCCATGATGAATTGCCACCAGTGCAACATCCTATTCACTATACATTTCAAAAAAAG; AATTCACATACTAAACAAAATTTCAGTTGTCTGAAAATGAAATGATTGAAAGTCTTTATG; AATCTCATACATACAATATGTGGCTAGCTGAAATTGTCTATCACGTAGCATTTAGATATA; AAAAGCCTCATGCTAGTTTGTTAAATGCAAAGGCTACCAGACGACCATTTAGCTGGAGAA; TATACGGAAGGCTTTCAGACAACGCACAGGTATAGTGCTGCTCACAGTGCAGGATGGTAG; AGGACTGAAACATGCAACCTTACACCTTACTTGGTAAAGCAGATTTAGTCTTCATGCCTG; GACTGAACTCCACAGCTGCTGTGTTTCAACCAACAGTAATTTAGACTTTTTGTAACAACA; ACCAATGTCTTTTTCTTCTTTAAGAAAAAAAGAATAAATTAATTACCTTTGGCCAATTAT; ATATCCCTTTTACATGTTTAAAATTGTGCCTTTGAACTCGCAAAATGGGTACTCTCTGCT; GAGAGAAGTTCCTGATAAATATATATTTATACCAAAGGCAGCTGGAAAATCTAGAATTTC; TTTGCCACAAGTTAAGAGAGATCTTAAACTGGCACTTACCCTGGAGAACTTTCCTAGGAA; ATCTAAAAAGGTTCTTCATCCCGGAAAAAACAAAAAAATTTTCCAATTTACCTTCACTCA; AAAAAACTATAATGGGCGCTTTTAATTTTTGCCCAACCTTCTGCATTTATTGGCTAACAC; CTGTCCAACTCCATTTGCCCAAATTCGCTGGGCCTCACCCCCCAAAAATGATGTCTTCCC; AAAAGGGCTTCTCCACCGAGGGCCCGTTTTCCCCACAAACAAAAGCCACAATTTTCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003941
• Insert Size: 4540 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003941.2, NP_003932.3
• RefSeq Size: 4430 bp
• RefSeq ORF: 1518 bp
• Locus ID: 8976
• UniProt ID: O00401
• Cytogenetics: 7q31.32
• Domains: PBD, WH1, WH2
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• Summary: This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]