SOCS1 (NM_003745) Human Untagged Clone

CAT#: SC111081

SOCS1 (untagged)-Human suppressor of cytokine signaling 1 (SOCS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SOCS1
• Synonyms: AISIMD; CIS1; CISH1; JAB; SOCS-1; SSI-1; SSI1; TIP-3; TIP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003745 edited
  ATCGGCACCAGGGCAGCTGCACGGCTCCTGGCCCCGGAGCATGCGCGAGAGCCGCCCCGG
  AGCGCCCCGGAGCCCCCCGCCGTCCCGCCCGCGGCGTCCCGCGCCCCGCCGCCAGCGCAC
  CCCCGGACGCTATGGCCCACCCCTCCGGCTGGCCCCTTCTGTAGGATGGTAGCACACAAC
  CAGGTGGCAGCCGACAATGCAGTCTCCACAGCAGCAGAGCCCCGACGGCGGCCAGAACCT
  TCCTCCTCTTCCTCCTCCTCGCCCGCGGCCCCCGCGCGCCCGCGGCCGTGCCCCGCGGTC
  CCGGCCCCGGCCCCCGGCGACACGCACTTCCGCACATTCCGTTCGCACGCCGATTACCGG
  CGCATCACGCGCGCCAGCGCGCTCCTGGACGCCTGCGGATTCTACTGGGGGCCCCTGAGC
  GTGCACGGGGCGCACGAGCGGCTGCGCGCCGAGCCCGTGGGCACCTTCCTGGTGCGCGAC
  AGCCGCCAGCGGAACTGCTTTTTCGCCCTTAGCGTGAAGATGGCCTCGGGACCCACGAGC
  ATCCGCGTGCACTTTCAGGCCGGCCGCTTTCACCTGGATGGCAGCCGCGAGAGCTTCGAC
  TGCCTCTTCGAGCTGCTGGAGCACTACGTGGCGGCGCCGCGCCGCATGCTGGGGGCCCCG
  CTGCGCCAGCGCCGCGTGCGGCCGCTGCAGGAGCTGTGCCGCCAGCGCATCGTGGCCACC
  GTGGGCCGCGAGAACCTGGCTCGCATCCCCCTCAACCCCGTCCTCCGCGACTACCTGAGC
  TCCTTCCCCTTCCAGATTTGACCGGCAGCGCCCGCCGTGCACGCAGCATTAACTGGGATG
  CCGTGTTATTTTGTTATTACTTGCCTGGAACCATGTGGGTACCCTCCCCGGCCTGGGTTG
  GAGGGAGCGGATGGGTGTAGGGGCGAGGCGCCTCCCGCCCTCGGCTGGAGACGAGGCCGC
  AGACCCCTTCTCACCTCTTGAGGGGGTCCTCCCCCTCCTGGTGCTCCCTCTGGGTCCCCC
  TGGTTGTTGTAGCAGCTTAACTGTATCTGGAGCCAGGACCTGAACTCGCACCTCCTACCT
  CTTCATGTTTACATATACCCAGTATCTTTGCACAAACCAGGGGTTGGGGGAGGGTCTCTG
  GCTTTATTTTTCTGCTGTGCAGAATCCTATTTTATATTTTTTAAAGTCAGTTTAGGTAAT
  AAACTTTATTATGAAAGTTTTTTTTTTAAAAAAAAAAAAAAAAAAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003745 unedited
  GTTCAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATCGGCACCAGGGCAGCT
  GCACGGCTCCTGGCCCCGGNACATGCGCGAGAGCCGCCCCGGAGCGCCCCGGAGCCCCCC
  GCCGTCCCGCCCGCGGCGTCCCGCGCCCCGCCGCCAGCGCACCCCCGGACGCTATGGCCC
  ACCCCTCCGGCTGGCCCCTTCTGTAGGATGGTAGCACACAACCAGGTGGCAGCCGACAAT
  GCAGTCTCCACAGCAGCAGAGCCCCGACGGCGGCCAGAACCTTCCTCCTCTTCCTCCTCC
  TCGCCCGCGGCCCCCGCGCGCCCGCGGCCGTGCCCCGCGGTCCCGGCCCCGGCCCCCGGC
  GACACGCACTTCCGCACATTCCGTTCGCACGCCGATTACCGGCGCATCACGCGCGCCAGC
  GCGCTCCTGGACGCCTGCGGATTCTACTGGGGGCCCCTGAGCGTGCACGGGGCGCACGAG
  CGGCTGCGCGCCGAGCCCGTGGGCACCTTCCTGGTGCGCGACAGCCGCCAGCGGAACTGC
  TTTTTCGCCCTTAGCGTGAAGATGGCCTCGGGACCCACGAGCATCCGCGTGCACTNTCAG
  GCCGGCCGCTTTCACCTGGATGGCAGCCGCGAGAGCTTCGACTGCCTCTTCGAGCTGCTG
  GAGCACTACGTGGCGGCGCCGCGCCGCATGCTGNGGGCCCCCGCTGCGCCAGCGCCGCGT
  GCGGCCGCTGCNAGAGCTGTGCCGCCAGCGCATCGTGGCCCACCGTGGGCCGCGAGAACC
  TGGCTCGCATCCNCCTCAAACCCGTNCTNCGNGACTACCTGAGCTNCTTNCCCTTNNCAG
  ATTGACCGNCAGCGCCCGCCGTGCACGCAGCATAACTGGNATGGCGGGTNTNATTTGTAT
  TACTTGCCTGGAACCCATGTGGGTACCCTCCCCGCCCTGGNNTGAGGNACGAATGNNTGT
  A
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003745
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003745.1, NP_003736.1
• RefSeq Size: 1216 bp
• RefSeq ORF: 636 bp
• Locus ID: 8651
• UniProt ID: O15524
• Cytogenetics: 16p13.13
• Domains: SH2, SOCS
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway
• Protein Pathways: Insulin signaling pathway, Jak-STAT signaling pathway, Type II diabetes mellitus, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008]