p60 CAF1 (CHAF1B) (NM_005441) Human Untagged Clone

CAT#: SC111066

CHAF1B (untagged)-Human chromatin assembly factor 1, subunit B (p60) (CHAF1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p60 CAF1
• Synonyms: CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60; MPHOSPH7; MPP7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005441, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAAGTCATCACTTGTGAAATAGCCTGGCACAACAAGGAGCCCGTGTACAGCCTGGACTTCCAGCATG
  GGACGGCTGGGAGGATCCACAGACTGGCGTCTGCCGGCGTGGACACCAATGTCAGGATCTGGAAGGTAGA
  AAAGGGACCAGATGGAAAAGCCATCGTGGAATTTTTGTCCAATCTTGCTCGTCATACCAAAGCCGTCAAT
  GTTGTGCGTTTTTCTCCAACTGGGGAAATTTTAGCATCGGGAGGAGATGATGCTGTCATCCTATTGTGGA
  AGGTGAATGATAACAAGGAGCCGGAGCAGATCGCTTTTCAGGATGAGGACGAGGCCCAGCTGAACAAGGA
  GAACTGGACGGTTGTGAAGACTCTGCGGGGCCACTTAGAAGATGTGTATGATATTTGCTGGGCAACTGAT
  GGGAATTTAATGGCTTCTGCCTCTGTGGATAACACAGCCATCATATGGGATGTCAGCAAAGGACAAAAGA
  TATCAATTTTTAATGAACATAAAAGTTATGTCCAAGGAGTAACCTGGGACCCTTTGGGTCAATATGTTGC
  TACTCTGAGCTGTGACAGGGTGCTGCGAGTATACAGTATACAGAAGAAGCGTGTGGCTTTCAATGTTTCG
  AAGATGCTGTCTGGAATAGGGGCTGAAGGAGAGGCAAGAAGCTACCGGATGTTTCACGACGACAGCATGA
  AGTCTTTCTTCCGTAGACTGAGTTTCACTCCCGACGGATCTTTGCTTCTCACGCCAGCTGGATGTGTGGA
  ATCTGGTGAAAATGTAATGAATACCACTTATGTTTTCTCCAGGAAGAATCTTAAAAGGCCCATCGCTCAT
  CTTCCATGTCCTGGAAAAGCCACTCTTGCTGTTCGCTGCTGTCCGGTCTACTTTGAACTGAGGCCAGTGG
  TGGAAACAGGTGTGGAGCTGATGAGTCTGCCCTACCGCCTGGTGTTTGCTGTGGCCTCGGAGGATTCCGT
  GCTTCTGTATGACACCCAGCAGTCCTTCCCTTTTGGTTACGTGTCTAATATACATTACCACACCCTCAGT
  GACATTTCATGGTCCAGCGATGGTGCCTTCCTGGCCATTTCTTCCACGGACGGTTACTGCTCATTTGTGA
  CATTTGAGAAAGATGAACTTGGAATTCCTTTGAAAGAGAAGCCAGTTTTGAACATGAGAACTCCTGATAC
  AGCAAAGAAAACCAAGAGTCAGACACATCGAGGGTCTTCGCCAGGACCCAGACCGGTAGAGGGAACCCCT
  GCCAGCAGAACCCAAGACCCCAGCAGCCCCGGCACGACTCCCCCTCAGGCCAGACAGGCCCCAGCCCCAA
  CAGTCATCAGGGACCCTCCCTCCATCACTCCTGCTGTCAAAAGCCCCTTGCCGGGGCCTTCGGAGGAGAA
  GACCCTGCAGCCCAGTAGTCAAAACACAAAAGCCCACCCATCCCGGAGGGTCACTCTGAACACACTGCAA
  GCCTGGAGCAAGACAACACCCCGGAGAATAAACTTAACACCCTTAAAGACGGACACTCCACCAAGTTCTG
  TACCAACCAGTGTGATTTCCACCCCTTCTACAGAAGAAATTCAGTCAGAGACGCCTGGAGACGCTCAGGG
  CAGTCCCCCAGAGCTAAAGCGGCCCAGACTCGATGAAAACAAAGGAGGCACGGAAAGTCTGGACCCTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005441 unedited
  GTAACGTACAAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGAAGC
  GGCGCGCGCTGCGCGGGAGGTGACGGTGCCTCTGACTGTCCGGGTCCCTCCAGCATTTTG
  CAGCTTTCTCCTGTCTTGAAGAAGTAGAACGGTGCCCGAGAAACGTTTTTCCCCTTCGAG
  ACTCAGGAGGATGAAAGTCATCACTTGTGAAATAGCCTGGCACAACAAGGAGCCCGTGTA
  CAGCCTGGACTTCCAGCATGGGACGGCTGGGAGGATCCACAGACTGGCGTCTGCCGGCGT
  GGACACCAATGTCAGGATCTGGAAGGTAGAAAAGGGACCAGATGGAAAAGCCATCGTGGA
  ATTTTTGTCCAATCTTGCTCGTCATACCAAAGCCGTCAATGTTGTGCGTTTTTCTCCAAC
  TGGGGAAATTTTAGCATCGGGAGGAGATGATGCTGTCATCCTATTGTGGAAGGTGAATGA
  TAACAAGGAGCCGGAGCAGATCGCTTTTCAGGATGAGGACGAGGCCCAGCTGAACAAGGA
  GAACTGGACGGTTGTGAAGACTCTGCGGGGCCACTTAGAAGATGTGTATGATATTTGCTG
  GGCAACTGATGGGAATTTAATGGCTTCTGCCTCTGTGGATAACACAGCCATCATATGGGA
  TGTCAGCAAAGGACAAAAGATATCAATTTTTAATGAACATAAAAGTTATGTCCAAGGAGT
  AACCTGGGACCCTTTGGGTCAATATGTTGCTACTCTGAGCTGTGACAGGGTGCTGCGAGT
  ATACAGTATACAGAAGAAGCGTGTGGCTTTCAATGTTTCGAAGATGCTGTCTGGAATAGG
  GGCTGAAGGAGAGGCAAGAAGCTACGGGATGTTTCACGACGACCGCATGAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005441 unedited
  GCACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTGCCTTCCAAATTTAATCAATCA
  ACTCCTTATAAGTGAAATCTAACAATCACTTCAAATACTCTAAAATTCGACAGAGTAAAA
  TCTCAAATTACTTTCTCATCTTCCTAATTCTTATTGAAAAGGAGTCCCTCCATGAAGTGT
  GTGCACTTTCACGAGGATGCCAGGGAGGACTCACTGATTTTCACACTGGATAACGTTGAA
  CCGCTCCAGTTTCAGAAACTCATCAAAAAGAGGATTTACTACGTTAAAACGTTCATGTCC
  CAAGTTAAAAATGGATACTGAATACAACAGCATCCATATTTCTGGAGAAAAATCAGTACA
  CATGTCACTTCTGTTATAGAAATCCATTTACAGTGTGTCCCGCTCCATGGAAGCCCTGGT
  CTCAAGCACCTCCAGCTTTACCGTCTCCCTGCACACACCGGGAGCCTGGTAGGCTTCGAG
  CAGAAGCCGAGGTCCCATCAAGGGTCCAGACTTTCCGTGCCTCCTTTGTTTTCATCGAGT
  CTGGGCCGCTTTAGCTCTGGGGGACTGACCTGAGCGTCTCCAGGCGTCTCTGACTGAATT
  TCTTCTGTAGAAGGAGTGGAAATCACACTGGTTGGTACAGAACTTGGTGGAGTGTCCGTC
  TTTAAAGGTGTTATAGTTATTCTNCGGGGTAGTGTCTTGCTCCAGGCTAGCAGAGTGTTC
  AAAGTGACCCTCCGGGATGGGTGGGCTTTTGTGNTTTGACTACTGGGCTGCAAGGTCTTC
  TCCTCCGAAGCCCCCGCAAGGGGCTTTGACAGCAGATGATGGAGGAAGGTCCTGATGACT
  GTGGGCTGGAGCTGTCTGCCTGAGGGATTCCGCCGGCTGCTGGTCTTTGTTCTGCTGGAA
  GGTTCCCTTACCGNCTTGGGCCGGCGAAACCCCAAGGGCTGACCTTGGTTTCTTGCGTAT
  AGGAATCCACGCAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005441
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005441.2, NP_005432.1
• RefSeq Size: 2297 bp
• RefSeq ORF: 1680 bp
• Locus ID: 8208
• UniProt ID: Q13112
• Cytogenetics: 21q22.12-q22.13
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]