MAG (NM_002361) Human Untagged Clone

CAT#: SC110943

MAG (untagged)-Human myelin associated glycoprotein (MAG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAG
• Synonyms: GMA; S-MAG; SIGLEC-4A; SIGLEC4A; SPG75
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110943 sequence for NM_002361 edited (data generated by NextGen Sequencing)
  ATGATATTCCTCACGGCACTGCCTCTGTTCTGGATTATGATTTCAGCCTCCCGAGGGGGT
  CACTGGGGTGCCTGGATGCCCTCGTCCATCTCGGCCTTCGAAGGCACGTGCGTCTCCATC
  CCCTGCCGCTTTGACTTCCCGGATGAGCTGCGGCCCGCTGTGGTGCATGGTGTCTGGTAC
  TTCAATAGCCCCTACCCCAAGAACTACCCCCCGGTGGTCTTCAAGTCGCGCACCCAAGTA
  GTCCACGAGAGCTTCCAGGGCCGCAGCCGCCTCCTGGGGGACCTGGGCCTGCGAAACTGC
  ACCCTCCTGCTCAGCAACGTCAGCCCCGAGCTGGGCGGGAAGTACTACTTCCGTGGGGAC
  CTGGGCGGCTACAACCAGTACACCTTCTCAGAGCACAGTGTCCTGGATATCGTCAACACC
  CCCAACATCGTGGTGCCCCCAGAGGTGGTGGCAGGCACGGAGGTGGAGGTCAGCTGCATG
  GTGCCGGACAACTGCCCAGAGCTGCGCCCTGAGCTGAGCTGGCTGGGCCACGAGGGGCTG
  GGGGAGCCCGCTGTGCTGGGCCGGCTGCGGGAGGACGAGGGCACCTGGGTGCAGGTGTCA
  CTGCTGCACTTCGTGCCCACGAGGGAGGCCAACGGCCACAGGCTGGGCTGCCAGGCCTCC
  TTCCCCAACACCACCCTGCAGTTCGAGGGCTACGCCAGCATGGACGTCAAGTACCCCCCG
  GTGATTGTGGAGATGAACTCCTCGGTGGAGGCCATCGAGGGCTCCCACGTGAGCCTGCTC
  TGTGGGGCTGACAGCAACCCCCCGCCGCTGCTGACCTGGATGCGGGACGGGACAGTCCTC
  CGGGAGGCGGTGGCCGAGAGCCTGCTCCTGGAGCTGGAGGAGGTGACCCCCGCCGAAGAC
  GGCGTCTATGCCTGCCTGGCCGAGAATGCCTATGGCCAGGACAACCGCACCGTGGGGCTC
  AGTGTCATGTATGCACCCTGGAAGCCAACAGTGAACGGGACAATGGTGGCCGTAGAGGGG
  GAGACGGTCTCTATCTTGTGCTCCACACAGAGCAACCCGGACCCTATTCTCACCATCTTC
  AAGGAGAAGCAGATCCTGTCCACGGTCATCTACGAGAGCGAGCTGCAGCTGGAGCTGCCG
  GCCGTGTCACCCGAGGATGATGGAGAGTACTGGTGTGTGGCTGAGAACCAGTATGGCCAG
  AGGGCCACCGCCTTCAACCTGTCTGTGGAGTTCGCCCCTGTGCTCCTCCTGGAGTCCCAC
  TGCGCGGCAGCCCGAGACACGGTGCAGTGCCTGTGCGTGGTGAAGTCCAACCCGGAGCCG
  TCCGTGGCCTTTGAGCTGCCATCGCGCAATGTGACCGTGAACGAGAGCGAGCGGGAGTTC
  GTGTACTCGGAGCGCAGCGGCCTCGTGCTCACCAGCATCCTCACGCTGCGGGGGCAGGCC
  CAGGCCCCGCCCCGCGTCATCTGCACCGCGAGGAACCTCTATGGCGCCAAGAGCCTGGAG
  CTGCCCTTCCAGGGAGCCCATCGACTGATGTGGGCCAAGATCGGGCCTGTGGGCGCCGTG
  GTCGCCTTTGCCATCCTGATTGCCATCGTCTGCTACATTACCCAGACACGCAGGAAAAAG
  AACGTGACAGAGAGCCCCAGCTTCTCGGCAGGGGACAACCCTCCCGTCCTGTTCAGCAGC
  GACTTCCGCATCTCTGGGGCACCAGAGAAGTACGAGAGCGAGAGGCGCCTGGGATCTGAG
  AGGAGGCTGCTGGGCCTTCGGGGTGAGCCCCCAGAGCTGGACCTGAGCTATTCTCACTCG
  GACCTGGGGAAACGGCCCACCAAGGACAGCTACACGCTGACGGAGGAGCTAGCTGAGTAT
  GCTGAAATCCGGGTCAAGTGA
  
  Clone variation with respect to NM_002361.3
  399 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002361 unedited
  GCAAACCCATTCGGCACGAGGCGGAGCAGATGTGCAGAAGCAACTGAGTCCAAGTTGTCT
  GGCGGCTTCAGGTGGACCCAGAAGACGTCCCCAACTCAGGGAGATTCAGCGATCACTCAC
  TCGCTGTACAGAATGATATTCCTCACGGCACTGCCTCTGTTCTGGATTATGATTTCAGCT
  CCCTCGTTGGGGTCACTGGGGTGCCTGGATGCCCTCGTCCATCTCGGCCTTCGAAGGCAC
  GTGCGTCTCCATCCCCTGCCGCTTTGACTTCCCGGATGAGCTGCGGCCCGCTGTGGTGCA
  TGGTGTCTGGTACTTCAATAGCCCCTACCCCAAGAACTACCCCCCGGTGGTCTTCAAGTC
  GCGCACCCAAGTAGTCCACGAGAGCTTCCAGGGCCGCAGCCGCCTCCTGGGGGACCTGGG
  CCTGCGAAACTGCACCCTCCTGCTCAGCAACGTCAGCCCCGAGCTGGGCGGGAAGTACTA
  CTTCCGTGGGGACCTGGGCGGCTACAACCAGTACACCTTCTCAGAGCACAGTGTCCTGGA
  TATCGTCAACACCCCCAACATCGTGGTGCCCCCAGAGGTGGTGGCAGGCACGGAGGTGGA
  GGTCAGCTGCATGGTGCCGGACAACTGCCCAGAGCTGCGCCCTGAGCTGAGCTGGCTGGG
  CCACGANGGGCTGGGGGAGCCCGCTGTGCTGGGCCCGCTGCGGGAGGACGAGGGCACCTG
  GGTGCANGTGTCACTGCTGCACTTCGTGCCCACGAGGGAGGCCAACCGCCACAGGCTGGG
  CTGCCAGGCCTCCTTTCCNCACACCACCCTGCAGTTCGAAGGCTACGCCAGCATGGACGT
  TCAGTACCCCCGGGTGATTGGGGAGATGAACTCCTCGTTGAGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002361 unedited
  ATGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGCTTTGGGGCA
  TTTATTTCATGTCCCTTTTGTACAGGCCCCAGGGCCAGGTGTGGGGGCAGGAGACCAGGC
  AGGAAGTAGCAATAAATAACACGGACAGACAGAGCTCCCCTCCAGACAGATGAGGGTAAA
  GGAGGTTAGAGAGGAGCCGTAATGAGGGCGTGGGGGTGCTGGCAGGGAGGGGCAGCTGGG
  AAGGAGGGGGAGGTCAGGCCCCCAGGACCTCACTGTCACCTGCCTCACTCCCCTCCTGCC
  CCAGCCCCCGATACTTTTGGGAGGAAGGGAGCCCACAGCCAGTGGGGGCCAGCGAGGGTC
  CTGAGGGGGGTATTTTTACCATTTCTCACCTTTTATTTCCCACCCCACCCCCTTCCCCCC
  CCCCCCTTCCTCCCTTTCCCCTCTCTTCGCCTCCCACCCCCCTCCCCCCCTTCCCCCCTT
  TTCCACCTATTTATTTTCTCAACCTCACTCACCTAGCCTCAGCCTCAGCTATTCACCCTG
  CGCAACCCTCCCTCCCCCCCCCCTCCCCCTATACCTCCTACTTTCTTTTATTTCCATCCC
  CCCCCCTCCCCCTCTCTCGCTTCCCCCCCCACAATAATTTATCAATTCACACTCGTCTGT
  GACCTTCCGCCGCACCCACCGCGAATAATATCCCCCCCCTCCCTCCCGCCTCCCGCCCCC
  ATCTTTATTATTATTCTCCCCCCCCTTTTCTCTGCATCACCCCACCCCTCCCATTTCTTC
  TCTTTATAAAGAAGTACCCTCTCCCCCCGCCGCCTCGCGATTACATTCTTTCTCCTCTCT
  CTCCCCCTCTGCACCTCTCTCATCATTAGCATCTCACTTCTTCTCTTCTCTTCGCTCGCG
  TACACCCCGAGATTTTATTCCGGCGTTCCATTTCGCCTCGCTCGCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002361
• Insert Size: 2440 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002361.2, NP_002352.1
• RefSeq Size: 2380 bp
• RefSeq ORF: 1881 bp
• Locus ID: 4099
• UniProt ID: P20916
• Cytogenetics: 19q13.12
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) lacks exon 11 and has the stop codon in exon 12, as compared to variant 2. Isoform a is 44 aa longer and contains a different carboxy terminus when compared to isoform b.