Eg5 (KIF11) (NM_004523) Human Untagged Clone

SKU SC110939

KIF11 (untagged)-Human kinesin family member 11 (KIF11)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Eg5
• Synonyms: EG5; HKSP; KNSL1; MCLMR; TRIP5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110939 sequence for NM_004523 edited (data generated by NextGen Sequencing); ATGGCGTCGCAGCCAAATTCGTCTGCGAAGAAGAAAGAGGAGAAGGGGAAGAACATCCAG; GTGGTGGTGAGATGCAGACCATTTAATTTGGCAGAGCGGAAAGCTAGCGCCCATTCAATA; GTAGAATGTGATCCTGTACGAAAAGAAGTTAGTGTACGAACTGGAGGATTGGCTGACAAG; AGCTCAAGGAAAACATACACTTTTGATATGGTGTTTGGAGCATCTACTAAACAGATTGAT; GTTTACCGAAGTGTTGTTTGTCCAATTCTGGATGAAGTTATTATGGGCTATAATTGCACT; ATCTTTGCGTATGGCCAAACTGGCACTGGAAAAACTTTTACAATGGAAGGTGAAAGGTCA; CCTAATGAAGAGTATACCTGGGAAGAGGATCCCTTGGCTGGTATAATTCCACGTACCCTT; CATCAAATTTTTGAGAAACTTACTGATAATGGTACTGAATTTTCAGTCAAAGTGTCTCTG; TTGGAGATCTATAATGAAGAGCTTTTTGATCTTCTTAATCCATCATCTGATGTTTCTGAG; AGACTACAGATGTTTGATGATCCCCGTAACAAGAGAGGAGTGATAATTAAAGGTTTAGAA; GAAATTACAGTACACAACAAGGATGAAGTCTATCAAATTTTAGAAAAGGGGGCAGCAAAA; AGGACAACTGCAGCTACTCTGATGAATGCATACTCTAGTCGTTCCCACTCAGTTTTCTCT; GTTACAATACATATGAAAGAAACTACGATTGATGGAGAAGAGCTTGTTAAAATCGGAAAG; TTGAACTTGGTTGATCTTGCAGGAAGTGAAAACATTGGCCGTTCTGGAGCTGTTGATAAG; AGAGCTCGGGAAGCTGGAAATATAAATCAATCCCTGTTGACTTTGGGAAGGGTCATTACT; GCCCTTGTAGAAAGAACACCTCATGTTCCTTATCGAGAATCTAAACTAACTAGAATCCTC; CAGGATTCTCTTGGAGGGCGTACAAGAACATCTATAATTGCAACAATTTCTCCTGCATCT; CTCAATCTTGAGGAAACTCTGAGTACATTGGAATATGCTCATAGAGCAAAGAACATATTG; AATAAGCCTGAAGTGAATCAGAAACTCACCAAAAAAGCTCTTATTAAGGAGTATACGGAG; GAGATAGAACGTTTAAAACGAGATCTTGCTGCAGCCCGTGAGAAAAATGGAGTGTATATT; TCTGAAGAAAATTTTAGAGTCATGAGTGGAAAATTAACTGTTCAAGAAGAGCAGATTGTA; GAATTGATTGAAAAAATTGGTGCTGTTGAGGAGGAGCTGAATAGGGTTACAGAGTTGTTT; ATGGATAATAAAAATGAACTTGACCAGTGTAAATCTGACCTGCAAAATAAAACACAAGAA; CTTGAAACCACTCAAAAACATTTGCAAGAAACTAAATTACAACTTGTTAAAGAAGAATAT; ATCACATCAGCTTTGGAAAGTACTGAGGAGAAACTTCATGATGCTGCCAGCAAGCTGCTT; AACACAGTTGAAGAAACTACAAAAGATGTATCTGGTCTCCATTCCAAACTGGATCGTAAG; AAGGCAGTTGACCAACACAATGCAGAAGCTCAGGATATTTTTGGCAAAAACCTGAATAGT; CTGTTTAATAATATGGAAGAATTAATTAAGGATGGCAGCTCAAAGCAAAAGGCCATGCTA; GAAGTACATAAGACCTTATTTGGTAATCTGCTGTCTTCCAGTGTCTCTGCATTAGATACC; ATTACTACAGTAGCACTTGGATCTCTCACATCTATTCCAGAAAATGTGTCTACTCATGTT; TCTCAGATTTTTAATATGATACTAAAAGAACAATCATTAGCAGCAGAAAGTAAAACTGTA; CTACAGGAATTGATTAATGTACTCAAGACTGATCTTCTAAGTTCACTGGAAATGATTTTA; TCCCCAACTGTGGTGTCTATACTGAAAATCAATAGTCAACTAAAGCATATTTTCAAGACT; TCATTGACAGTGGCCGATAAGATAGAAGATCAAAAAAAGGAACTAGATGGCTTTCTCAGT; ATACTGTGTAACAATCTACATGAACTACAAGAAAATACCATTTGTTCCTTGGTTGAGTCA; CAAAAGCAATGTGGAAACCTAACTGAAGACCTGAAGACAATAAAGCAGACCCATTCCCAG; GAACTTTGCAAGTTAATGAATCTTTGGACAGAGAGATTCTGTGCTTTGGAGGAAAAGTGT; GAAAATATACAGAAACCACTTAGTAGTGTCCAGGAAAATATACAGCAGAAATCTAAGGAT; ATAGTCAACAAAATGACTTTTCACAGTCAAAAATTTTGTGCTGATTCTGATGGCTTCTCA; CAGGAACTCAGAAATTTTAACCAAGAAGGTACAAAATTGGTTGAAGAATCTGTGAAACAC; TCTGATAAACTCAATGGCAACCTGGAAAAAATATCTCAAGAGACTGAACAGAGATGTGAA; TCTCTGAACACAAGAACAGTTTATTTTTCTGAACAGTGGGTATCTTCCTTAAATGAAAGG; GAACAGGAACTTCACAACTTATTGGAGGTTGTAAGCCAATGTTGTGAGGCTTCAAGTTCA; GACATCACTGAGAAATCAGATGGACGTAAGGCAGCTCATGAGAAACAGCATAACATTTTT; CTTGATCAGATGACTATTGATGAAGATAAATTGATAGCACAAAATCTAGAACTTAATGAA; ACCATAAAAATTGGTTTGACTAAGCTTAATTGCTTTCTGGAACAGGATCTGAAACTGGAT; ATCCCAACAGGTACGACACCACAGAGGAAAAGTTATTTATACCCATCAACACTGGTAAGA; ACTGAACCACGTGAACATCTCCTTGATCAGCTGAAAAGGAAACAGCCTGAGCTGTTAATG; ATGCTAAACTGTTCAGAAAACAACAAAGAAGAGACAATTCCGGATGTGGATGTAGAAGAG; GCAGTTCTGGGGCAGTATACTGAAGAACCTCTAAGTCAAGAGCCATCTGTAGATGCTGGT; GTGGATTGTTCATCAATTGGCGGGGTTCCATTTTTCCAGCATAAAAAATCACATGGAAAA; GACAAAGAAAACAGAGGCATTAACACACTGGAGAGGTCTAAAGTGGAAGAAACTACAGAG; CACTTGGTTACAAAGAGCAGATTACCTCTGCGAGCCCAGATCAACCTTTAA; ; Clone variation with respect to NM_004523.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004523 unedited; ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGATTCGGGCG; GAGACGAGATTAGTGATTTGGCGGCTCCGACTGGCGCGGGACAAACGCCACGGCCAGAGT; ACCGGGTAGAGAGCGGGGACGCCGACCTGCGTGCGTCGGTCCTCCAGGCCACGCCAGCGC; CCGAGAGGGACCAGGGAGACTCCGGCCCCTGTCGGCCGCCAAGCCCCTCCGCCCCTCACA; GCGCCCAGGTCCGCGGCCGGGCCTTGATTTTTTGGCGGGGACCGTCATGGCGTCGCAGCC; AAATTCGTCTGCGAAGAAGAAAGAGGAGAAGGGGAAGAACATCCAGGTGGTGGTGAGATG; CAGACCATTTAATTTGGCAGAGCGGAAAGCTAGCGCCCATTCAATAGTAGAATGTGATCC; TGTACGAAAAGAAGTTAGTGTACGAACTGGAGGATTGGCTGACAAGAGCTCAAGGAAAAC; ATACACTTTTGATATGGTGTTTGGAGCATCTACTAAACAGATTGATGTTTACCGAAGTGT; TGTTTGTCCAATTCTGGATGAAGTTATTATGGGCTATAATTGCACTATCTTTGCGTATGG; CCAAACTGGCACTGGAAAAACTTTTACAATGGAAGGTGAAAGGTCACCTAATGAAGAGTA; TACCTGGGAAGAGGATCCCTTGGCTGGTATAATTCCACGTACCCTTCATCAAATTTTTGA; GAAACTTACTGANTATGGTACTGGATTTTCAGTCAAAGTGTCTCTGTGGGAGATCTANTA; TGAAGAGCTTTTTGATCTTTCTAATCCATCATCTGATGGTTCTGAGAGACTACAGATGTT; TGATGATCCCCCGTACAGAGAGAGTGANTATTAAAGGTTAGAAGAATACCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004523
• Insert Size: 3870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004523.2, NP_004514.2
• RefSeq Size: 4908 bp
• RefSeq ORF: 3171 bp
• Locus ID: 3832
• UniProt ID: P52732
• Cytogenetics: 10q23.33
• Domains: kinesin
• Protein Families: Druggable Genome
• Summary: This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]