Ku70 (XRCC6) (NM_001469) Human Untagged Clone

SKU SC110901

XRCC6 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Ku70
• Synonyms: CTC75; CTCBF; G22P1; KU70; ML8; TLAA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110901 sequence for NM_001469 edited (data generated by NextGen Sequencing); ATGTCAGGGTGGGAGTCATATTACAAAACCGAGGGCGATGAAGAAGCAGAGGAAGAACAA; GAAGAGAACCTTGAAGCAAGTGGAGACTATAAATATTCAGGAAGAGATAGTTTGATTTTT; TTGGTTGATGCCTCCAAGGCTATGTTTGAATCTCAGAGTGAAGATGAGTTGACACCTTTT; GACATGAGCATCCAGTGTATCCAAAGTGTGTACATCAGTAAGATCATAAGCAGTGATCGA; GATCTCTTGGCTGTGGTGTTCTATGGTACCGAGAAAGACAAAAATTCAGTGAATTTTAAA; AATATTTACGTCTTACAGGAGCTGGATAATCCAGGTGCAAAACGAATTCTAGAGCTTGAC; CAGTTTAAGGGGCAGCAGGGACAAAAACGTTTCCAAGACATGATGGGCCACGGATCTGAC; TACTCACTCAGTGAAGTGCTGTGGGTCTGTGCCAACCTCTTTAGTGATGTCCAATTCAAG; ATGAGTCATAAGAGGATCATGCTGTTCACCAATGAAGACAACCCCCATGGCAATGACAGT; GCCAAAGCCAGCCGGGCCAGGACCAAAGCCGGTGATCTCCGAGATACAGGCATCTTCCTT; GACTTGATGCACCTGAAGAAACCTGGGGGCTTTGACATATCCTTGTTCTACAGAGATATC; ATCAGCATAGCAGAGGATGAGGACCTCAGGGTTCACTTTGAGGAATCCAGCAAGCTAGAA; GACCTGTTGCGGAAGGTTCGCGCCAAGGAGACCAGGAAGCGAGCACTCAGCAGGTTAAAG; CTGAAGCTCAACAAAGATATAGTGATCTCTGTGGGCATTTATAATCTGGTCCAGAAGGCT; CTCAAGCCTCCTCCAATAAAGCTCTATCGGGAAACAAATGAACCAGTGAAAACCAAGACC; CGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGCGATACCAAGAGGTCTCAG; ATCTATGGGAGTCGTCAGATTATACTGGAGAAAGAGGAAACAGAAGAGCTAAAACGGTTT; GATGATCCAGGTTTGATGCTCATGGGTTTCAAGCCGTTGGTACTGCTGAAGAAACACCAT; TACCTGAGGCCCTCCCTGTTCGTGTACCCAGAGGAGTCGCTGGTGATTGGGAGCTCAACC; CTGTTCAGTGCTCTGCTCATCAAGTGTCTGGAGAAGGAGGTTGCAGCATTGTGCAGATAC; ACACCCCGCAGGAACATCCCTCCTTATTTTGTGGCTTTGGTGCCACAGGAAGAAGAGTTG; GATGACCAGAAAATTCAGGTGACTCCTCCAGGCTTCCAGCTGGTCTTTTTACCCTTTGCT; GATGATAAAAGGAAGATGCCCTTTACTGAAAAAATCATGGCAACTCCAGAGCAGGTGGGC; AAGATGAAGGCTATCGTTGAGAAGCTTCGCTTCACATACAGAAGTGACAGCTTTGAGAAC; CCCGTGCTGCAGCAGCACTTCAGGAACCTGGAGGCCTTGGCCTTGGATTTGATGGAGCCG; GAACAAGCAGTGGACCTGACATTGCCCAAGGTTGAAGCAATGAATAAAAGACTGGGCTCC; TTGGTGGATGAGTTTAAGGAGCTTGTTTACCCACCAGATTACAATCCTGAAGGGAAAGTT; ACCAAGAGAAAACACGATAATGAAGGTTCTGGAAGCAAAAGGCCCAAGGTGGAGTATTCA; GAAGAGGAGCTGAAGACCCACATCAGCAAGGGTACGCTGGGCAAGTTCACTGTGCCCATG; CTGAAAGAGGCCTGCCGGGCTTACGGGCTGAAGAGTGGGCTGAAGAAGCAGGAGCTGCTG; GAAGCCCTCACCAAGCACTTCCAGGACTGA; ; Clone variation with respect to NM_001469.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001469 unedited; TTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGGTCGCTTCCC; TGCGCCAAAGTGAGCAGTAGCCAACATGTCAGGGTGGGAGTCATATTACAAAACCGAGGG; CGATGAAGAAGCAGAGGAAGAACAAGAAGAGAACCTTGAAGCAAGTGGAGACTATAAATA; TTCAGGAAGAGATAGTTTGATTTTTTTGGTTGATGCCTCCAAGGCTATGTTTGAATCTCA; GAGTGAAGATGAGTTGACACCTTTTGACATGAGCATCCAGTGTATCCAAAGTGTGTACAT; CAGTAAGATCATAAGCAGTGATCGAGATCTCTTGGCTGTGGTGTTCTATGGTACCGAGAA; AGACAAAAATTCAGTGAATTTTAAAAATATTTACGTCTTACAGGAGCTGGATAATCCAGG; TGCAAAACGAATTCTAGAGCTTGACCAGTTTAAGGGGCAGCAGGGACAAAAACGTTTCCA; AGACATGATGGGCCACGGATCTGACTACTCACTCAGTGAAGTGCTGTGGGTCTGTGCCAA; CCTCTTTAGTGATGTCCAATTCAAGATGAGTCATAAGAGGATCATGCTGTTCACCAATGA; AGACAACCCCCATGGCAATGACAGTGCCAAAGCCAGCCGGGCCAGGACCAAAGCCGGTGA; TCTCCGAGATACAGGCATCTTCCTTGACTTGATGCACCTGAAGAAACCTGGGGGCTNTGA; CATATNNCTTGNTCTACAGAGATATCATCAGCATAGCAGAGGATGANGGACCTCAGGGNT; TCACTTTNGAGAAATCCAGCAGCTANAAGACCTGTTGCCGGAAGGGTCGCGCCAAGGAGA; CCCAGAAGCGAGCACTCAGCAGGTTAAAGCTGAAGCTCACAAAGATTTATGATCTCTTGG; GCATTTATATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001469 unedited; CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTATAGTACAAAC; TTAGGGCTCTTTATTCAGGCAGTAAAGTAAGGAACAGCAAAGTGGGAGGGCTACACCATC; ACCATGGCAACAGAAAGCCTCAAAAACATAAAGTCCCTCGACTTATGTCGGGTAGACTCT; TCCTAGCTCAGGAGAAACACATTTTAACTGGCTGAGGACAAGGCCAGGCAGCCTGGCCAC; ACTGCGGAAGGGCAGCTGGACGCGCGGCCTCTGGTCAGTCCTGGAAGTGCTTGGTGAGGG; CTTCCAGCAGCTCCTGCTTCTTCAGCCCACTCTTCAGCCCGTAAGCCCGGCAGGCCTCTT; TCAGCATGGGCACAGTGAACTTGCCCAGCGTACCCTTGCTGATGTGGGTCTTCAGCTCCT; CTTCTGAATACTCCACCTTGGGCCTTTTGCTTCCAGAACCTTCATTATCGTGTTTTCTCT; TGGTAACTTTCCCTTCAGGATTGTAATCTGGTGGGTAAACAAGCTCCTTAAACTCATCCA; CCAAGGAGCCCAGTCTTTTATTCATTGCTTCAACCTTGGGCAATGTCAGGTCCACTGCTT; GTTCCGGCTCCATCAAATCCAAGGCCAAGGCCTNCAGGTTCCTGAAGTGCTGCTGCAGCA; CGGNGTTCTCANAGCTGTCACTTCTGTATGTGAAGCGAAGCTTCTCAACGATAGCCTTCA; TCTTGCCCACCTGCGTCTGGAGTGCCATGATTNTTTCAGTAAAGGGCATCTTCCTTTTAT; CATCAGCAAAGGGTAAAAAGACCAGCTGGAAGCCTGGAGGAGTCACCTNNGAATTTCTGG; TCATCCAACTCTTNCTTCTGTGGGCACCAAAGCCACAATAAGGAGGGATGTNCCTGCGGG; TGTGTATCTGCACATGCTGCACCCTCTTTNCAGACACTGAGAGCANACACTGNACAGGTT; GAGCNCCCATACAGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001469
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001469.3, NP_001460.1
• RefSeq Size: 2156 bp
• RefSeq ORF: 1830 bp
• Locus ID: 2547
• UniProt ID: P12956
• Cytogenetics: 22q13.2
• Domains: ku, Ku_C, Ku_N, SAP
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Non-homologous end-joining
• Summary: The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Variants 1 and 2 encode the same protein.