MUS81 (NM_025128) Human Untagged Clone

CAT#: SC110891

MUS81 (untagged)-Human MUS81 endonuclease homolog (S. cerevisiae) (MUS81)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MUS81
• Synonyms: SLX3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110891 sequence for NM_025128 edited (data generated by NextGen Sequencing)
  ATGGCGGCCCCGGTCCGCCTGGGCCGGAAGCGCCCGCTGCCTGCCTGTCCCAACCCGCTC
  TTCGTTCGCTGGCTGACCGAGTGGCGGGACGAGGCGACCCGCAGCAGGCACCGCACGCGC
  TTCGTATTTCAGAAGGCGCTGCGTTCCCTCCGACGGTACCCACTGCCGCTGCGCAGCGGG
  AAGGAAGCTAAGATCCTACAGCACTTCGGAGACGGGCTCTGCCGGATGCTGGACGAGCGG
  CTGCAGCGGCACCGAACATCGGGCGGTGACCATGCCCCGGACTCACCATCTGGAGAGAAC
  AGTCCAGCCCCGCAGGGGCGACTTGCGGAAGTCCAGGACTCTTCCATGCCAGTTCCTGCC
  CAGCCCAAAGCGGGAGGCTCTGGCAGCTACTGGCCAGCTCGGCACTCAGGAGCCCGAGTG
  ATACTGCTGGTGCTCTACCGGGAGCACCTGAATCCTAATGGTCACCACTTCTTAACCAAG
  GAGGAGCTGCTGCAGAGGTGTGCTCAGAAGTCCCCCAGGGTAGCCCCTGGGAGTGCCCCA
  CCCTGGCCAGCCCTCCGCTCCCTCCTTCACAGGAACCTGGTCCTCAGGACACACCAGCCA
  GCCAGGTACTCATTGACCCCAGAGGGCCTGGAGCTGGCCCAGAAGTTGGCCGAGTCAGAA
  GGCCTGAGCTTGCTGAATGTGGGCATCGGGCCCAAGGAGCCCCCTGGGGAGGAGACAGCA
  GTGCCAGGAGCAGCTTCAGCAGAGCTTGCCAGTGAAGCAGGGGTCCAGCAGCAGCCACTG
  GAGCTGAGGCCTGGAGAGTACAGGGTGCTGTTGTGTGTGGACATTGGCGAGACCCGGGGG
  GGCGGGCACAGGCCGGAGCTGCTCCGAGAGCTACAGCGGCTGCACGTGACCCACACGGTG
  CGCAAGCTGCACGTTGGAGATTTTGTGTGGGTGGCTCAGGAGACCAATCCTAGAGACCCA
  GCAAACCCTGGGGAGTTGGTACTGGATCACATTGTGGAGCGCAAGCGACTGGATGACCTT
  TGCAGCAGCATCATCGACGGCCGCTTCCGGGAGCAGAAGTTCCGACTGAAGCGCTGTGGT
  CTGGAGCGCCGGGTATACCTGGTGGAAGAGCATGGTTCCGTCCACAACCTCAGCCTTCCT
  GAGAGCACACTGCTGCAGGCTGTCACCAACACTCAGGTCATTGATGGCTTTTTTGTGAAG
  CGCACAGCAGACATTAAGGAGTCAGCCGCCTACCTGGCCCTCTTGACTCGGGGCCTGCAG
  AGACTCTACCAGGGCCACACCCTACGCAGCCGCCCCTGGGGAACCCCTGGGAACCCTGAA
  TCAGGGGCCATGACCTCTCCAAACCCTCTCTGCTCACTCCTCACCTTCAGTGACTTCAAC
  GCAGGAGCCATCAAGAATAAGGCCCAGTCGGTGCGAGAAGTGTTTGCCCGGCAGCTGATG
  CAGGTGCGCGGAGTGAGTGGGGAGAAGGCAGCAGCCCTGGTGGATCGATACAGCACCCCT
  GCCAGCCTCCTGGCCGCCTATGATGCCTGTGCCACCCCCAAGGAACAAGAGACACTGCTG
  AGCACCATTAAGTGTGGGCGTCTACAGAGGAATCTGGGGCCTGCTCTGAGCAGGACCTTA
  TCCCAGCTCTACTGCAGCTACGGCCCCTTGACCTGA
  
  Clone variation with respect to NM_025128.4
  110 g=>a;537 t=>c;539 g=>c;936 c=>t;1065 g=>a;1248 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025128 unedited
  GTATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGGTGGGAGGGCGGCCGCAG
  GCTCTCCTCTCGTTAGTGCCCCCTGTGTTTGGGGCCCCGTGATCTCAACGGTCCTGCCCT
  CGGTCTCCCTCTTCCCCCGCCCCGCCCTGGGCCAGGTGTTCGAATCCCGACTCCAGAACT
  GGCGGCGTCCCAGTCCCGCGGGCGTGGAGCGCCGGAGGACCCGCCCTCGGGCTCATGGCG
  GCCCCGGTCCGCCTGGGCCGGAAGCGCCCGCTGCCTGCCTGTCCCAACCCGCTCTTCGTT
  CGCTGGCTGACCGAGTGGCGGGACGAGGCGACCCGCAGCAGGCACCGCACGCGCTTCGTA
  TTTCAGAAGGCGCTGCGTTCCCTCCGACGGTACCCACTGCCGCTGCGCAGCGGGAAGGAA
  GCTAAGATCCTACAGCACTTCGGAGACGGGCTCTGCCGGATGCTGGACGAGCGGCTGCAG
  CGGCACCGAACATCGGGCGGTGACCATGCCCCGGACTCACCATCTGGAGAGAACAGTCCA
  GCCCCGCAGGGGCGACTTGCGGAAGTCCAGGACTCTTCCATGCCAGTTCCTGCCCAGCCC
  AAAGCGGGAGGCTCTGGCAGCTACTGGCCAGCTCGGCACTCAGGAGCCCGAGTGATACTG
  CTGGTGCTCTANCCGGGAGCACCTGATCCTAATGGTCACCACTTCTTACCCAAGGAGGAG
  CTGCTGCAGAGGTGTGCTCANAAAGTCCCCCAGGTAGCCCCTGGGAGTGCCCCACCCTGG
  NCAGGCCTTCGNTCCCTTCTTNACAGGAACCCTGTTNCTCAGACACACAGCCAGCCAGTA
  CTCATTGACCCCAGAGGCCTGNAGCTTGCCCANAAGTTGCCGAGTCAAAGGCCTGAGCTG
  CTGATGTGGGCATCGGGCCCAGGAGCCCCTGGGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025128 unedited
  ATGGCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTCTAATGGCCCCCCAAAAAATGTTGTTAAAAGGCTGC
  CTACCCTGGGTTGGGGAACAAACGGGGGCTGGGGGCTTTTTCACGGCATAACCTCAGGCC
  AAGGGGCCGAACCTGCAAAAAACCTGGAATAAGGCCCTGCTCAAAGCAGGCCCCAAATTC
  CTTTGTAAACCCCCACCCTTAAGGGGGCTCACCAGGGCTTTTTGTTCCTTGGGGGGGGCA
  CAGGCATTATAGGCGGCCAAAAGGCTGGCAGGGGTGCTTTATCAATCCACCAGGGCTGTT
  GCTTTTTCCCCACTCACTCCGGGCACCTGAATAAGTTGCCGGGCAAACACTTTTCGCACC
  GACTGGGCCTTATTCTTGAGGGCTCCTGCGTTAAAGTCACTGAAGGGGAGGAGTGACCAA
  AAAGGGTTTGGAAAGGTCATGGCCCCTGATTCAGGGTTCCCAGGGGTTCCCCAGGGGCGG
  CTGCGTAGGGTGTGGCCCTGGTAAAGTCTCTGCAGGCCCCGATTCAAAAGGGCCAGGTAG
  GCGGCTGACTCCTTAATGGCTGCTGCGCGCTTAACAAAAAACCCATCAATGACCTTGAGT
  GTTTGGAGAACACCCTGCAACCAGTGGCCCCTTCAAAGAAGGCCTGAGGTTTTGTGAACG
  AGAACCATGTCTCTTCCACCAGTGTTAACCCGGCGGCTCCAAACCACAAGGCTTAAATCG
  GAAATTTTTGCTCCCGAAACCGCCCGCCAATAAGCTTGTTAAAAGCCATCCAACCGTCGC
  CCCCCCCAAGTGAACCACCCCACCTCCCCAGGGTTGGCGGCCCCTAGGATGGTCCTCGAC
  CCCCCCCCAATTTCCACGGGCACTGCCCCCGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025128
• Insert Size: 2280 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025128.3, NP_079404.2
• RefSeq Size: 2343 bp
• RefSeq ORF: 1656 bp
• Locus ID: 80198
• UniProt ID: Q96NY9
• Cytogenetics: 11q13.1
• Domains: ERCC4
• Protein Pathways: Homologous recombination
• Gene Summary: This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks. [provided by RefSeq, Jun 2017]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.