Adenylosuccinate Lyase (ADSL) (NM_000026) Human Untagged Clone

SKU SC110822

ADSL (untagged)-Human adenylosuccinate lyase (ADSL), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Adenylosuccinate Lyase
• Synonyms: AMPS; ASASE; ASL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000026, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCTGGAGGCGATCATGGTTCGCCCGACAGCTACCGCTCACCTCTTGCCTCCCGCTATGCCAGCC; CGGAGATGTGCTTCGTGTTTAGCGACAGGTATAAATTCCGGACATGGCGGCAGCTGTGGCTGTGGCTGGC; GGAGGCCGAGCAGACATTGGGTTTGCCTATCACAGATGAACAAATCCAGGAGATGAAATCAAACCTGGAG; AACATCGACTTCAAGATGGCAGCTGAGGAAGAGAAACGTTTACGACATGATGTGATGGCTCACGTGCACA; CATTTGGCCACTGCTGTCCAAAAGCTGCAGGCATTATTCACCTTGGTGCTACTTCTTGCTATGTTGGAGA; CAATACTGACTTGATTATTCTTAGAAATGCACTTGACCTGCTTTTGCCAAAGCTTGCCAGAGTGATCTCT; CGGCTTGCCGACTTTGCTAAGGAACGAGCCAGTCTACCCACATTAGGTTTCACACATTTCCAGCCTGCAC; AGCTGACCACAGTTGGGAAACGTTGCTGTCTTTGGATTCAGGATCTTTGCATGGATCTCCAGAACTTGAA; GCGTGTCCGAGATGACCTGCGCTTCCGGGGAGTAAAGGGTACCACTGGCACTCAGGCCAGTTTCCTGCAG; CTCTTTGAGGGAGATGACCATAAGGTAGAGCAGCTTGACAAGATGGTGACAGAAAAGGCAGGATTTAAGA; GAGCTTTCATCATCACAGGGCAGACATATACACGAAAAGTGGATATTGAAGTACTGTCTGTGCTGGCTAG; CTTGGGGGCATCAGTGCACAAGATTTGCACCGACATACGCCTCCTGGCAAACCTCAAGGAGATGGAGGAA; CCCTTTGAAAAACAGCAGATTGGCTCAAGTGCGATGCCATATAAGCGGAATCCCATGCGTTCAGAACGTT; GCTGCAGTCTTGCCCGCCACCTGATGACCCTTGTCATGGACCCGCTACAGACAGCATCTGTCCAGTGGTT; TGAACGCACACTGGATGATAGTGCCAACCGACGGATCTGTTTGGCCGAGGCATTTCTTACCGCAGATACT; ATATTGAATACGCTGCAGAACATTTCTGAAGGATTGGTCGTGTACCCCAAAGTAATTGAACGGCGCATTC; GGCAAGAGCTGCCTTTCATGGCCACAGAGAACATCATCATGGCCATGGTCAAAGCTGGAGGTAGCCGCCA; GGATTGCCATGAGAAAATCAGAGTGCTTTCTCAGCAGGCAGCTTCTGTGGTTAAGCAGGAAGGGGGTGAC; AATGACCTCATAGAGCGTATCCAGGTTGATGCCTACTTCAGTCCCATTCACTCCCAGTTGGATCATTTAC; TGGATCCTTCTTCTTTCACTGGTCGTGCCTCCCAGCAGGTGCAGAGATTCTTAGAAGAGGAGGTGTATCC; CCTGTTAAAACCATATGAAAGCGTGATGAAGGTGAAAGCAGAATTATGTCTGTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000026 unedited; CGTCAGATTTGAACTCGACTCATATAGGCGGCCGCTNAATCGGCACGAGGCCTCGTGCCG; AATTCGGCACGAGGCAGGGTTGGGATGGCGGCTGGAGGCGATCATGGTTCGCCCGACAGC; TACCGCTCACCTCTTGCCTCCCGCTATGCCAGCCCGGAGATGTGCTTCGTGTTTAGCGAC; AGGTATAAATTCCGGACATGGCGGCAGCTGTGGCTGTGGCTGGCGGAGGCCGAGCAGACA; TTGGGTTTGCCTATCACAGATGAACAAATCCAGGAGATGAAATCAAACCTGGAGAACATC; GACTTCAAGATGGCAGCTGAGGAAGAGAAACGTTTACGACATGATGTGATGGCTCACGTG; CACACATTTGGCCACTGCTGTCCAAAAGCTGCAGGCATTATTCACCTTGGTGCTACTTCT; TGCTATGTTGGAGACAATACTGACTTGATTATTCTTAGAAATGCACTTGACCTGCTTTTG; CCAAAGCTTGCCAGAGTGATCTCTCGGCTTGCCGACTTTGCTAAGGAACGAGCCAGTCTA; CCCACATTAGGTTTCACACATTTCCAGCCTGCACAGCTGACCACAGTTGGGAAACGTTGC; TGTCTTTGGATTCAGGATCTTTGCATGGATCTCCAGAACTTGAAGCGTGTCCGAGATGAC; CTGCGCTTCCGGGGAGTAAAGGGTACCACTGGCACTCAGGCCAGTTTCCTGCAGCTCTTT; GAGGGAGATGACCATAANGTAGAGCAGCTTGACTAGATGGTGACAGACAAGGCAGGATTT; TAGAGAGCTTTCATCATCACAGGGCAGACATATACACGAAAAGTGGATAATGCAGTACTG; TCTGTGCTGGCTAGCTTGNGGNCATCAGTGCACAGATTTGCACTGACTACCCCTCTGCCA; AACTCAGGAGATGTAGCACCCTTTGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000026 unedited; TCGACATATACAATGATTTTCGTTTAATTCTCTTCCAACTCTACATACATAATTCTGCTT; TCACCTTCATCACGCTTTCATATGGTTTTAACAGGGGATACACCTCCTCTTCTAAGAATC; TCTGCACCTGCTGGGAGGCACGACCAGTGAAAGAAGAAGGATCCAGTAAATGATCCAACT; GGGAGTGAATGGGACTGAAGTAGGCATCAACCTGGATACGCTCTATGAGGTCATTGTCAC; CCCCTTCCTGCTTAACCACAGAAGCTGCCTGCTGAGAAAGCACTCTGATTTTCTCATGGC; AATCCTGGCGGCTACCTCCAGCTTTGACCATGGCCATGATGATGTTCTCTGTGGCCATGA; AAGGCAGCTCTTGCCGAATGCGCCGTTCAATTACTTTGGGGTACACGACCAATCCTTCAG; AAATGTTCTGCAGCGTATTCAATATAGTATCTGCGGTAAGAAATGCCTCGGCCAAACAGA; TCCGTCGGTTGGCACTATCATCCAGTGTGCGTTCAAACCACTGGACAGATGCTGTCTGTA; GCGGGTCCATGACAAGGGTCATCATGTGGCGGGCAAGACTGCAGCAACGTTCTGAACGCA; TGGGATTCCGCTTATATGGCATCGCACTTGAGCCAATCTGCTGTTTTTCAAAGGGTTCCT; CCATCTNCTTGAGGTTGCCAGGAGGCGTATGTCGGTGCAAATCTTGTGCACTGATGCCCC; CAAGCTAGCCAGCACAGACAGTACTTAATATCCACTTTCCTGTATATGTCTGCCCTGTGA; TGATGATAGCTCTCTTTAATCCTGCCTTTTCTGTCACCATCTTGTCAAGCTGCTCTACCT; TATGGTCATTTCCCTAAAGGACTGCAAGAAACTGGCCCTGATGCCATTGTTTCCCTTTTC; TCCCAGGATCGCAGTTATCTCCGGCACGCTTAATGTCTGGAAATCCTCCAAATTCCTGAT; CCAAACACGTACGTTCCAACTGGGGCACN;
• Restriction Sites: NotI-NotI
• ACCN: NM_000026
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000026.1, NP_000017.1
• RefSeq Size: 1692 bp
• RefSeq ORF: 1455 bp
• Locus ID: 158
• UniProt ID: P30566
• Cytogenetics: 22q13.1
• Domains: lyase_1
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism
• Summary: The protein encoded by this gene belongs to the lyase 1 family. It is an essential enzyme involved in purine metabolism, and catalyzes two non-sequential reactions in the de novo purine biosynthetic pathway: the conversion of succinylaminoimidazole carboxamide ribotide (SAICAR) to aminoimidazole carboxamide ribotide (AICAR) and the conversion of adenylosuccinate (S-AMP) to adenosine monophosphate (AMP). Mutations in this gene are associated with adenylosuccinase deficiency (ADSLD), a disorder marked with psychomotor retardation, epilepsy or autistic features. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]; Transcript Variant: This variant (1) represents the predominant transcript and encodes the longest isoform (a).