MED28 (NM_025205) Human Untagged Clone

CAT#: SC110380

MED28 (untagged)-Human mediator complex subunit 28 (MED28)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MED28
• Synonyms: 1500003D12Rik; EG1; magicin
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_025205, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCTCCACTAGGGGGTATGTTTTCTGGGCAGCCACCCGGTCCCCCTCAGGCCCCGCCGGGCCTTC
  CGGGCCAAGCTTCGCTTCTTCAGGCAGCTCCAGGCGCTCCTAGACCTTCCAGCAGTACTTTGGTGGACGA
  GTTGGAGTCATCTTTCGAGGCTTGCTTTGCATCTCTGGTGAGTCAGGACTATGTCAATGGCACCGATCAG
  GAAGAAATTCGAACCGGTGTTGATCAGTGTATCCAGAAGTTTCTGGATATTGCAAGACAGACAGAATGTT
  TTTTCTTACAAAAAAGATTGCAGTTATCTGTCCAGAAACCAGAGCAAGTTATCAAAGAGGATGTGTCAGA
  ACTAAGGAATGAATTACAGCGGAAAGATGCACTAGTCCAGAAGCACTTGACAAAGCTGAGGCATTGGCAG
  CAGGTGCTGGAGGACATCAACGTGCAGCACAAAAAGCCCGCCGACATCCCTCAGGGCTCCTTGGCCTACC
  TGGAGCAGGCATCTGCCAACATCCCTGCACCTCTGAAGCCAACGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025205 unedited
  CATTCAGAATTGTAAACGACTCATATAGGCGGCCGCGAATCGGCACGAGCAAACATGGCG
  GCTCCACTAGGGGGTATGTTTTCTGGGCAGCCACCCGGTCCCCCTCAGGCCCCGCCGGGC
  CTTCCGGGCCAAGCTTCGCTTCTTCAGGCAGCTCCAGGCGCTCCTAGACCTTCCAGCAGT
  ACTTTGGTGGACGAGTTGGAGTCATCTTTCGAGGCTTGCTTTGCATCTCTGGTGAGTCAG
  GACTATGTCAATGGCACCGATCAGGAAGAAATTCGAACCGGTGTTGATCAGTGTATCCAG
  AAGTTTCTGGATATTGCAAGACAGACAGAATGTTTTTTCTTACAAAAAAGAGTGCAGTTA
  TCTGTCCAGAAACCAGAGCAAGTTATCAAAGAGGATGTGTCAGAACTAAGGAATGAATTA
  CAGCGGAAAGATGCACTAGTCCAGAAGCACTTGACACAGCTGAGGCATTGGCAGCAGGTG
  CTGGAGGACATCAACGTGCAGCACAAAAAAGCCGCCGACATCCCTCAGGGCTCCTTGGCC
  TACCTGGAGCAGGCATCTGCCAACATCCCTGCACCTCTGAAGCCAACGTGAGCAAAGGGC
  ACAAGCAGTTGGCCTATGACTGGGCTGATGCCTGAGGTTTGCCCCACATTCCTTTCTGGG
  GACATGCCTTTTGGCAGGACTCTTTGCCAGAAAATGGAGTCATTTTAGGTTTAGGCCCCC
  ATGGGAAAATTTCCCCCTTTTTTAAAAGCGGGTAATTTTTTGGGGCCCTTTAACCAAGTC
  TTGACCTTGGAAAACCCACAAAAGATTTTTTTTTTGGGTTTTACCGCAATTTAAACTGGG
  AAATTAAAACCCCCAAACTTTTTTAGGGGGCCTTGCTTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025205 unedited
  NTTTTTGCTCTGGAACCGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTGGC
  AAAAAAACTTCTGAATTTAATAAAGAAAGGCAACAACTGGTACGTACAGTAATTCAAAAT
  CTTTTCACTAAGCACCAAATAGGATAGTTCTCAATCTTCTCAAGAACCTCTTTGAAAGAA
  CGCCTAAAACCTAATCAGTTTATTTAGGTTTTTAGTCAAGAAATACAAAGCTGCCAATCA
  GAGTTTCTAATTACCATGAGTGACCAGGTTAGCAACCTTGAGGTCATACCACATGAGCTT
  TTAAAAGCTTTGTTTAACCTATACGTTGTAACTGAGGGGATTTGTTGATGTCAGGCTGGT
  AATCAGAGATCCATGTGCCAGATACCATATACACACACCAAAAAGATACAGATTATATGG
  TCCTTATATATTTAAGGAGAATAATGTCTAATTCTCATGGGTGTAGAATTAGTGAGGGGC
  AGAGAGACAGATACAAAGCACTAACCCACCAGGGAGAACCTAGCGAGGGCAACAGAAGAG
  ATCAAAGTCCTGTGCCCTGGGGATGCAACTGAATGGGTGCTGGGAGGCTTCAGGGATCAG
  GCACATGAGTGGATGTCAGTCCATGTGGGAAGGGGCCTTCAAGGCTGAAGACAACAAAAT
  GAGTCAAGGCACAAAGCCACAGGAGTACTGCCAATCAGAAGACAGTGAAGCATTTGTTCT
  GGGTAAAGGGATGTGATCTAATAAGATATAAAACTCAGAAAACCTTACCNCATGAGAGCC
  AGAAATCTCCATTTCTTAACAGCAACTCAACAGGGAAATCTGAGCTGCCCACAAAACTAA
  CGAGAGCAAAATACCCCTNCCAAGGCAGGGCACCACATNGCTCTTTGTGGAGGCTCAGTA
  GCCAATGATCACCCTGCAGCCCCTNCCCNACCCCACACGAAACTCTAGTCCCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025205
• Insert Size: 2350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025205.3, NP_079481.2
• RefSeq Size: 1363 bp
• RefSeq ORF: 537 bp
• Locus ID: 80306
• UniProt ID: Q9H204
• Cytogenetics: 4p15.32
• Protein Families: Transcription Factors
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.[UniProtKB/Swiss-Prot Function]